(Circular) (Six-base) MAPSORT of: pFN21AB8853.seq Check: 2777 from: 1 to: 4933 pFN21AB8853 4933 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2743 278 Size: 53 83 186 936 1207 2468 Fragments arranged by size: 2468 1207 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2276 1937 Size: 339 4594 Acc65I G'GTAC_C Cuts at: 2254 2254 Size: 4933 AclI AA'CG_TT Cuts at: 1563 3060 3433 1563 Size: 1497 373 3063 Fragments arranged by size: 3063 1497 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2028 2996 4380 4648 1632 Size: 396 968 1384 268 1917 Fragments arranged by size: 1917 1384 968 396 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4736 AflIII A'CryG_T Cuts at: 1203 3832 4809 1203 Size: 2629 977 1327 Fragments arranged by size: 2629 1327 977 AgeI A'CCGG_T Cuts at: 3764 3764 Size: 4933 AhdI GACnn_n'nnGTC Cuts at: 3662 3662 Size: 4933 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 4933 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 4933 AlwNI CAG_nnn'CTG Cuts at: 2676 4248 2676 Size: 1572 3361 ApaLI G'TGCA_C Cuts at: 2990 4146 2990 Size: 1156 3777 ApoI r'AATT_y Cuts at: 1493 2242 2468 2620 4729 1493 Size: 749 226 152 2109 1697 Fragments arranged by size: 2109 1697 749 226 152 AseI AT'TA_AT Cuts at: 160 1032 3487 160 Size: 872 2455 1606 Fragments arranged by size: 2455 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 4933 AvaI C'yCGr_G Cuts at: 1870 1943 2258 1870 Size: 73 315 4545 Fragments arranged by size: 4545 315 73 BamHI G'GATC_C Cuts at: 2263 2630 2263 Size: 367 4566 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2254 3709 618 Size: 325 217 627 467 1455 1842 Fragments arranged by size: 1842 1455 627 467 325 217 BanII G_rGCy'C Cuts at: 1391 2252 1391 Size: 861 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 3962 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3124 3158 1903 Size: 34 1187 34 3678 Fragments arranged by size: 3678 1187 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2825 4035 2825 Size: 1210 3723 BclI T'GATC_A Cuts at: 1548 1548 Size: 4933 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3544 136 Size: 107 122 71 631 605 1872 1525 Fragments arranged by size: 1872 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4928 4928 Size: 4933 BlpI GC'TnA_GC Cuts at: 2350 2350 Size: 4933 Bme1580I G_kGCm'C Cuts at: 1790 2994 4150 1790 Size: 1204 1156 2573 Fragments arranged by size: 2573 1204 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 4933 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2664 3622 442 Size: 416 53 83 399 24 1247 958 1753 Fragments arranged by size: 1753 1247 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 4933 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3593 1239 Size: 2354 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3058 3923 4221 3058 Size: 865 298 3770 Fragments arranged by size: 3770 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3596 882 Size: 617 2097 2219 Fragments arranged by size: 2219 2097 617 BsaAI yAC'GTr Cuts at: 493 1204 2724 493 Size: 711 1520 2702 Fragments arranged by size: 2702 1520 711 BsaBI GATnn'nnATC Cuts at: 4927 4927 Size: 4933 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2740 3122 3757 275 Size: 53 83 186 936 1207 382 635 1451 Fragments arranged by size: 1451 1207 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3366 3764 4038 4185 4596 3366 Size: 398 274 147 411 3703 Fragments arranged by size: 3703 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4708 Fragments arranged by size: 4708 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 4933 BseYI C'CCAG_C Cuts at: 4136 4632 4136 Size: 496 4437 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4360 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2383 3144 4172 1540 Size: 275 186 382 761 1028 2301 Fragments arranged by size: 2301 1028 761 382 275 186 BsiHKAI G_wGCw'C Cuts at: 2994 3079 4150 2994 Size: 85 1071 3777 Fragments arranged by size: 3777 1071 85 BsmI GAATG_Cn' Cuts at: 2444 2537 2444 Size: 93 4840 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2252 2994 3079 4150 1391 Size: 399 462 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 462 399 85 BspEI T'CCGG_A Cuts at: 4596 4596 Size: 4933 BspHI T'CATG_A Cuts at: 2822 4552 2822 Size: 1730 3203 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2123 2288 844 Size: 1279 165 3489 Fragments arranged by size: 3489 1279 165 BsrBI CCG'CTC Cuts at: 1394 1823 2820 1394 Size: 429 997 3507 Fragments arranged by size: 3507 997 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2116 3428 3602 65 Size: 1183 868 1312 174 1396 Fragments arranged by size: 1396 1312 1183 868 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3577 3764 1700 Size: 110 1767 187 2869 Fragments arranged by size: 2869 1767 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 4933 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 4933 BssSI C'ACGA_G Cuts at: 2993 4005 2993 Size: 1012 3921 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 4933 BstBI TT'CG_AA Cuts at: 3737 3737 Size: 4933 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 4933 BstYI r'GATC_y Cuts at: 1972 2263 2630 3015 3032 4484 4570 4582 4928 Size: 291 367 385 17 1452 86 12 346 Cuts at: 4928 1972 Size: 1977 Fragments arranged by size: 1977 1452 385 367 346 291 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4381 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4194 Fragments arranged by size: 4194 683 56 BtsI GCAGTG_nn' Cuts at: 2449 3243 3263 2449 Size: 794 20 4119 Fragments arranged by size: 4119 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 4933 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4898 DraI TTT'AAA Cuts at: 2237 2237 Size: 4933 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3940 809 Size: 507 2624 1802 Fragments arranged by size: 2624 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2380 3269 4898 Size: 54 1425 50 275 18 550 889 1629 Cuts at: 4898 8 Size: 43 Fragments arranged by size: 1629 1425 889 550 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2380 1537 Size: 275 568 4090 Fragments arranged by size: 4090 568 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2863 1353 Size: 1510 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3520 3894 4040 387 Size: 1064 138 1931 374 146 1280 Fragments arranged by size: 1931 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2028 2996 3593 4380 4648 1239 Size: 393 396 968 597 787 268 1524 Fragments arranged by size: 1524 968 787 597 396 393 268 EcoRI G'AATT_C Cuts at: 2242 2620 2242 Size: 378 4555 EcoRV GAT'ATC Cuts at: 2080 2143 2080 Size: 63 4870 FspI TGC'GCA Cuts at: 3439 3439 Size: 4933 HaeII r_GCGC'y Cuts at: 1678 4080 1678 Size: 2402 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3574 3606 3648 3680 1373 Size: 32 135 32 2002 32 42 32 2626 Fragments arranged by size: 2626 2002 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2277 2523 669 Size: 988 281 339 246 3079 Fragments arranged by size: 3079 988 339 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 4933 HpaI GTT'AAC Cuts at: 2523 2523 Size: 4933 KpnI G_GTAC'C Cuts at: 2258 2258 Size: 4933 MfeI C'AATT_G Cuts at: 2532 2532 Size: 4933 MluI A'CGCG_T Cuts at: 4809 4809 Size: 4933 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4047 4231 1305 Size: 2742 184 2007 Fragments arranged by size: 2742 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3111 Fragments arranged by size: 3111 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2891 3250 3409 518 Size: 648 1725 359 159 2042 Fragments arranged by size: 2042 1725 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2349 3026 4174 4419 1606 Size: 743 677 1148 245 2120 Fragments arranged by size: 2120 1148 743 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4381 NdeI CA'TA_TG Cuts at: 387 2026 387 Size: 1639 3294 NheI G'CTAG_C Cuts at: 1052 1052 Size: 4933 NotI GC'GGCC_GC Cuts at: 2380 2380 Size: 4933 NspI r_CATG'y Cuts at: 1132 1229 2291 3836 1132 Size: 97 1062 1545 2229 Fragments arranged by size: 2229 1545 1062 97 PasI CC'CwG_GG Cuts at: 1348 2206 1348 Size: 858 4075 PciI A'CATG_T Cuts at: 3832 3832 Size: 4933 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 4933 PmeI GTTT'AAAC Cuts at: 2237 2237 Size: 4933 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3002 3034 4515 4547 3002 Size: 32 1481 32 3388 Fragments arranged by size: 3388 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 4933 PsiI TTA'TAA Cuts at: 2503 2503 Size: 4933 PstI C_TGCA'G Cuts at: 830 2285 830 Size: 1455 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 4933 SalI G'TCGA_C Cuts at: 1936 2275 1936 Size: 339 4594 SbfI CC_TGCA'GG Cuts at: 2285 2285 Size: 4933 ScaI AGT'ACT Cuts at: 3181 4893 3181 Size: 1712 3221 SfcI C'TryA_G Cuts at: 826 1045 2184 2281 3416 4097 4288 826 Size: 219 1139 97 1135 681 191 1471 Fragments arranged by size: 1471 1139 1135 681 219 191 97 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 4933 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 4933 SmaI CCC'GGG Cuts at: 1872 2260 1872 Size: 388 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3037 3938 4200 820 Size: 197 926 1094 901 262 1553 Fragments arranged by size: 1553 1094 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2724 493 Size: 2231 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 4933 SphI G_CATG'C Cuts at: 1132 2291 1132 Size: 1159 3774 SspI AAT'ATT Cuts at: 5 52 2857 5 Size: 47 2805 2081 Fragments arranged by size: 2805 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 4933 StyI C'CwwG_G Cuts at: 513 1065 2372 513 Size: 552 1307 3074 Fragments arranged by size: 3074 1307 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2960 2977 3130 3315 3760 325 Size: 1255 1380 17 153 185 445 1498 Fragments arranged by size: 1498 1380 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3179 4891 96 Size: 274 80 33 51 1443 1202 1712 138 Fragments arranged by size: 1712 1443 1202 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4893 4925 4893 Size: 32 4901 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 4933 XbaI T'CTAG_A Cuts at: 2269 2269 Size: 4933 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 4933 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 4933 XmaI C'CCGG_G Cuts at: 1870 2258 1870 Size: 388 4545 XmnI GAAnn'nnTTC Cuts at: 3062 3062 Size: 4933 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2741 276 Size: 53 83 186 936 1207 2468 Fragments arranged by size: 2468 1207 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI