Restriction map for
pFN21AB8846
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspI AviII
AvrII BamHI BbeI BbsI BcgI
BclI BfrI BlnI BlpI Bpu1102I
BpuAI BsaBI BsgI BsiWI BsmBI
Bsp1407I BspDI BspHI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstPI
BstXI CelII Cfr10I ClaI CpoI
DraIII EagI EclXI Eco47III Eco52I
EcoO65I EcoRI EcoT22I FbaI FseI
FspI HaeII HincII HindII HpaI
KasI KpnI KspI MamI MfeI
MluI MunI NaeI NarI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccIII 512 TCCGGA T^CCGGA
AcsI 1032 GAATTT [GA]^AATT[CT]
AcyI 508 GGCGTC G[GA]^CG[CT]C
AhdI 529 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 649 CCTCAGG CC^T[ACGT]AGG
ApoI 1032 GAATTT [GA]^AATT[CT]
Asp700I 516 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 529 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 152 TGGCCA TGG^CCA
BanI 871 GGCACC G^G[CT][GA]CC
BbvCI 320 CCTCAGC CCTCAGC(-5/-2)
BciVI 693 GTATCC GTATCC(6/5)
BcnI 1475 CCCGG CC^[GC]GG
BglII 833 AGATCT A^GATCT
BpmI 532 CTCCAG CTGGAG(16/14)
BsaHI 508 GGCGTC G[GA]^CG[CT]C
BsaWI 512 TCCGGA [AT]^CCGG[AT]
BseAI 512 TCCGGA T^CCGGA
BsiEI 1239 CGACCG CG[GA][CT]^CG
BsmI 1377 GCATTC GAATGC(1/-1)
BspEI 512 TCCGGA T^CCGGA
BspLU11I 621 ACATGT A^CATGT
BsrBI 902 GAGCGG CCGCTC(-3/-3)
BsrDI 935 CATTGC GCAATG(2/0)
BssHII 777 GCGCGC G^CGCGC
Bsu36I 649 CCTCAGG CC^T[ACGT]AGG
DraI 337 TTTAAA TTT^AAA
DraII 812 AGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 814 GACCTAGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 152 TGGCCA [CT]^GGCC[GA]
Eam1105I 529 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 649 CCTCAGG CC^T[ACGT]AGG
EcoO109I 812 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 163 GATATC GAT^ATC
HgaI 509 GCGTC GACGC(5/10)
Hin1I 508 GGCGTC G[GA]^CG[CT]C
HindIII 1254 AAGCTT A^AGCTT
MluNI 152 TGGCCA TGG^CCA
MroI 512 TCCGGA T^CCGGA
MscI 152 TGGCCA TGG^CCA
MspA1I 156 CAGCTG C[AC]G^C[GT]G
NciI 1475 CCCGG CC^[GC]GG
NspI 621 ACATGT [GA]CATG^[CT]
PpuMI 812 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1349 GACATACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 156 CAGCTG CAG^CTG
SfcI 393 CTACAG C^T[GA][CT]AG
SfiI 410 GGCCTCTGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 467 AGGCCT AGG^CCT
TfiI 118 GATTC G^A[AT]TC
XcmI 1162 CCAATCACACGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 516 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 402 CGCG CG^CG
778 CGCG
AflIII 621 ACATGT A^C[GA][CT]GT
1168 ACACGT
AlwNI 1501 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
1552 CAGAAACTG
BbrPI 546 CACGTG CAC^GTG
1003 CACGTG
BglI 411 GCCTCTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1000 GCCCACGTGGC
BsaAI 546 CACGTG [CT]AC^GT[GA]
1003 CACGTG
Bst1107I 666 GTATAC GTA^TAC
1530 GTATAC
BstUI 402 CGCG CG^CG
778 CGCG
BstYI 833 AGATCT [GA]^GATC[CT]
1435 GGATCT
BstZ17I 666 GTATAC GTA^TAC
1530 GTATAC
EcoNI 697 CCTTTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1113 CCTTACAGAGG
HapII 513 CCGG C^CGG
1476 CCGG
HpaII 513 CCGG C^CGG
1476 CCGG
MflI 833 AGATCT [GA]^GATC[CT]
1435 GGATCT
MspI 513 CCGG C^CGG
1476 CCGG
MvnI 402 CGCG CG^CG
778 CGCG
NheI 93 GCTAGC G^CTAGC
1191 GCTAGC
PmaCI 546 CACGTG CAC^GTG
1003 CACGTG
PmlI 546 CACGTG CAC^GTG
1003 CACGTG
SapI 573 GAAGAGC GCTCTTC(1/4)
731 GCTCTTC
SfaNI 443 GCATC GCATC(5/9)
603 GCATC
SmlI 764 CTCAAG C^T[CT][GA]AG
1257 CTTGAG
XhoII 833 AGATCT [GA]^GATC[CT]
1435 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AspHI AvaI AvaII BanII BbvI
BfaI BmyI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EarI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HhaI HinP1I HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MvaI
MwoI NcoI NdeII NlaIII NlaIV
PleI RsaI SacI Sau3AI Sau96I
ScrFI StyI Tru9I TseI Tsp45I
Tsp509I TspRI