(Circular) (Six-base) MAPSORT of: pFN21AB8846.seq Check: 1146 from: 1 to: 6310 pFN21AB8846 6310 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3481 3481 Size: 6310 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4120 278 Size: 53 83 186 936 2584 2468 Fragments arranged by size: 2584 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2672 2811 3536 3653 1937 Size: 735 139 725 117 4594 Fragments arranged by size: 4594 735 725 139 117 Acc65I G'GTAC_C Cuts at: 3631 3631 Size: 6310 AclI AA'CG_TT Cuts at: 1563 4437 4810 1563 Size: 2874 373 3063 Fragments arranged by size: 3063 2874 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2597 2965 4373 5757 6025 1632 Size: 965 368 1408 1384 268 1917 Fragments arranged by size: 1917 1408 1384 965 368 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6113 AflIII A'CryG_T Cuts at: 1203 2626 3173 5209 6186 1203 Size: 1423 547 2036 977 1327 Fragments arranged by size: 2036 1423 1327 977 547 AgeI A'CCGG_T Cuts at: 5141 5141 Size: 6310 AhdI GACnn_n'nnGTC Cuts at: 2539 5039 2539 Size: 2500 3810 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6310 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6310 AlwNI CAG_nnn'CTG Cuts at: 3511 3562 4053 5625 3511 Size: 51 491 1572 4196 Fragments arranged by size: 4196 1572 491 51 ApaLI G'TGCA_C Cuts at: 4367 5523 4367 Size: 1156 5154 ApoI r'AATT_y Cuts at: 1493 3037 3619 3845 3997 6106 1493 Size: 1544 582 226 152 2109 1697 Fragments arranged by size: 2109 1697 1544 582 226 152 AseI AT'TA_AT Cuts at: 160 1032 4864 160 Size: 872 3832 1606 Fragments arranged by size: 3832 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6310 AvaI C'yCGr_G Cuts at: 1870 1943 2494 2792 2902 3635 1870 Size: 73 551 298 110 733 4545 Fragments arranged by size: 4545 733 551 298 110 73 BamHI G'GATC_C Cuts at: 3640 4007 3640 Size: 367 5943 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2876 3631 5086 618 Size: 325 217 627 1089 755 1455 1842 Fragments arranged by size: 1842 1455 1089 755 627 325 217 BanII G_rGCy'C Cuts at: 1391 2297 2359 2585 2903 3629 1391 Size: 906 62 226 318 726 4072 Fragments arranged by size: 4072 906 726 318 226 62 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5339 BbvCI CC'TCA_GC Cuts at: 2326 2326 Size: 6310 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4501 4535 1903 Size: 34 2564 34 3678 Fragments arranged by size: 3678 2564 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2709 4202 5412 2709 Size: 1493 1210 3607 Fragments arranged by size: 3607 1493 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6310 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2422 3011 4921 Size: 107 122 71 631 605 750 589 1910 Cuts at: 4921 136 Size: 1525 Fragments arranged by size: 1910 1525 750 631 605 589 122 107 71 BglII A'GATC_T Cuts at: 2838 6305 2838 Size: 3467 2843 BlpI GC'TnA_GC Cuts at: 3727 3727 Size: 6310 Bme1580I G_kGCm'C Cuts at: 1790 2282 4371 5527 1790 Size: 492 2089 1156 2573 Fragments arranged by size: 2573 2089 1156 492 BmgBI CAC'GTC Cuts at: 1318 2350 2379 1318 Size: 1032 29 5249 Fragments arranged by size: 5249 1032 29 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3169 3467 4041 Size: 416 53 83 399 24 1752 298 574 Cuts at: 4041 4999 442 Size: 958 1753 Fragments arranged by size: 1753 1752 958 574 416 399 298 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2102 3200 1056 Size: 1046 1098 4166 Fragments arranged by size: 4166 1098 1046 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2522 4970 1239 Size: 1283 2448 2579 Fragments arranged by size: 2579 2448 1283 Bpu10I CC'TnA_GC Cuts at: 2326 2326 Size: 6310 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2754 3283 4435 5300 5598 2754 Size: 529 1152 865 298 3466 Fragments arranged by size: 3466 1152 865 529 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2435 2540 2595 3432 4973 882 Size: 617 936 105 55 837 1541 2219 Fragments arranged by size: 2219 1541 936 837 617 105 55 BsaAI yAC'GTr Cuts at: 493 1204 2553 3010 4101 493 Size: 711 1349 457 1091 2702 Fragments arranged by size: 2702 1349 1091 711 457 BsaBI GATnn'nnATC Cuts at: 6304 6304 Size: 6310 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2514 4117 4499 5134 Size: 53 83 186 936 981 1603 382 635 Cuts at: 5134 275 Size: 1451 Fragments arranged by size: 1603 1451 981 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2517 4743 5141 5415 5562 5973 2517 Size: 2226 398 274 147 411 2854 Fragments arranged by size: 2854 2226 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2300 2330 2710 2740 1178 Size: 30 165 30 897 30 380 30 4748 Fragments arranged by size: 4748 897 380 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2308 2311 2314 2453 2757 3140 1186 Size: 1122 3 3 139 304 383 4356 Fragments arranged by size: 4356 1122 383 304 139 3 3 BseYI C'CCAG_C Cuts at: 5513 6009 5513 Size: 496 5814 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5737 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3247 3760 4521 5549 1540 Size: 275 186 1246 513 761 1028 2301 Fragments arranged by size: 2301 1246 1028 761 513 275 186 BsiHKAI G_wGCw'C Cuts at: 2297 2359 2585 3309 4371 4456 5527 2297 Size: 62 226 724 1062 85 1071 3080 Fragments arranged by size: 3080 1071 1062 724 226 85 62 BsmI GAATG_Cn' Cuts at: 3382 3821 3914 3382 Size: 439 93 5778 Fragments arranged by size: 5778 439 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2282 2297 2359 2585 2903 3309 3629 Size: 399 492 15 62 226 318 406 320 Cuts at: 3629 4371 4456 5527 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 492 406 399 320 318 226 85 62 15 BspEI T'CCGG_A Cuts at: 2517 5973 2517 Size: 3456 2854 BspHI T'CATG_A Cuts at: 4199 5929 4199 Size: 1730 4580 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2453 2613 3481 3665 844 Size: 1609 160 868 184 3489 Fragments arranged by size: 3489 1609 868 184 160 BsrBI CCG'CTC Cuts at: 1394 1823 2909 4197 1394 Size: 429 1086 1288 3507 Fragments arranged by size: 3507 1288 1086 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2939 4805 4979 65 Size: 1183 1691 1866 174 1396 Fragments arranged by size: 1866 1691 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4954 5141 1700 Size: 110 3144 187 2869 Fragments arranged by size: 3144 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6310 BssHII G'CGCG_C Cuts at: 1925 2782 1925 Size: 857 5453 BssSI C'ACGA_G Cuts at: 4370 5382 4370 Size: 1012 5298 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6310 BstBI TT'CG_AA Cuts at: 5114 5114 Size: 6310 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6310 BstYI r'GATC_y Cuts at: 1972 2838 3440 3640 4007 4392 4409 5861 5947 Size: 866 602 200 367 385 17 1452 86 Cuts at: 5947 5959 6305 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 866 602 385 367 346 200 86 17 12 BstZ17I GTA'TAC Cuts at: 2673 3537 2673 Size: 864 5446 Bsu36I CC'TnA_GG Cuts at: 2655 2655 Size: 6310 BtgI C'CryG_G Cuts at: 513 1065 2411 2745 2880 3102 3277 513 Size: 552 1346 334 135 222 175 3546 Fragments arranged by size: 3546 1346 552 334 222 175 135 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2079 487 Size: 683 56 853 4718 Fragments arranged by size: 4718 853 683 56 BtsI GCAGTG_nn' Cuts at: 3826 4620 4640 3826 Size: 794 20 5496 Fragments arranged by size: 5496 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6310 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6275 DraI TTT'AAA Cuts at: 2344 3614 2344 Size: 1270 5040 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2825 5317 809 Size: 507 1509 2492 1802 Fragments arranged by size: 2492 1802 1509 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2157 3757 4646 Size: 54 1425 50 275 18 327 1600 889 Cuts at: 4646 6275 8 Size: 1629 43 Fragments arranged by size: 1629 1600 1425 889 327 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3757 1537 Size: 275 1945 4090 Fragments arranged by size: 4090 1945 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2491 2573 2743 4240 1353 Size: 1138 82 170 1497 3423 Fragments arranged by size: 3423 1497 1138 170 82 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2208 4897 5271 5417 387 Size: 1064 138 619 2689 374 146 1280 Fragments arranged by size: 2689 1280 1064 619 374 146 138 EcoICRI GAG'CTC Cuts at: 2295 2357 2583 2295 Size: 62 226 6022 Fragments arranged by size: 6022 226 62 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2522 2597 2965 4373 4970 5757 6025 Size: 393 890 75 368 1408 597 787 268 Cuts at: 6025 1239 Size: 1524 Fragments arranged by size: 1524 1408 890 787 597 393 368 268 75 EcoNI CCTnn'n_nnAGG Cuts at: 2706 3122 2706 Size: 416 5894 EcoO109I rG'GnC_Cy Cuts at: 2818 2818 Size: 6310 EcoRI G'AATT_C Cuts at: 3619 3997 3619 Size: 378 5932 EcoRV GAT'ATC Cuts at: 2170 2170 Size: 6310 FspI TGC'GCA Cuts at: 4816 4816 Size: 6310 HaeII r_GCGC'y Cuts at: 1678 5457 1678 Size: 3779 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2300 2332 2525 2557 2710 Size: 32 135 32 728 32 193 32 153 Cuts at: 2710 2742 2896 2928 3035 3067 4951 4983 5025 Size: 32 154 32 107 32 1884 32 42 Cuts at: 5025 5057 1373 Size: 32 2626 Fragments arranged by size: 2626 1884 728 193 154 153 135 107 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3654 3900 669 Size: 988 281 1716 246 3079 Fragments arranged by size: 3079 1716 988 281 246 HindIII A'AGCT_T Cuts at: 748 3259 748 Size: 2511 3799 HpaI GTT'AAC Cuts at: 3900 3900 Size: 6310 KpnI G_GTAC'C Cuts at: 3635 3635 Size: 6310 MfeI C'AATT_G Cuts at: 3909 3909 Size: 6310 MluI A'CGCG_T Cuts at: 6186 6186 Size: 6310 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5424 5608 1305 Size: 4119 184 2007 Fragments arranged by size: 4119 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2159 10 Size: 54 1425 343 327 4161 Fragments arranged by size: 4161 1425 343 327 54 MslI CAynn'nnrTG Cuts at: 518 1166 2453 2619 2919 3107 3312 3491 4268 Size: 648 1287 166 300 188 205 179 777 Cuts at: 4268 4627 4786 518 Size: 359 159 2042 Fragments arranged by size: 2042 1287 777 648 359 300 205 188 179 166 159 MspA1I CmG'CkG Cuts at: 1606 2163 3726 4403 5551 5796 1606 Size: 557 1563 677 1148 245 2120 Fragments arranged by size: 2120 1563 1148 677 557 245 NcoI C'CATG_G Cuts at: 513 1065 2745 3102 3277 513 Size: 552 1680 357 175 3546 Fragments arranged by size: 3546 1680 552 357 175 NdeI CA'TA_TG Cuts at: 387 387 Size: 6310 NheI G'CTAG_C Cuts at: 1052 2098 3196 1052 Size: 1046 1098 4166 Fragments arranged by size: 4166 1098 1046 NotI GC'GGCC_GC Cuts at: 3757 3757 Size: 6310 NspI r_CATG'y Cuts at: 1132 1229 2630 3668 5213 1132 Size: 97 1401 1038 1545 2229 Fragments arranged by size: 2229 1545 1401 1038 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6310 PciI A'CATG_T Cuts at: 2626 5209 2626 Size: 2583 3727 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6310 PmeI GTTT'AAAC Cuts at: 3614 3614 Size: 6310 PmlI CAC'GTG Cuts at: 2553 3010 2553 Size: 457 5853 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4379 4411 5892 5924 4379 Size: 32 1481 32 4765 Fragments arranged by size: 4765 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2818 2818 Size: 6310 PshAI GACnn'nnGTC Cuts at: 1613 3358 1613 Size: 1745 4565 PsiI TTA'TAA Cuts at: 3880 3880 Size: 6310 PstI C_TGCA'G Cuts at: 830 3662 830 Size: 2832 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6310 PvuII CAG'CTG Cuts at: 2163 2163 Size: 6310 SacI G_AGCT'C Cuts at: 2297 2359 2585 2297 Size: 62 226 6022 Fragments arranged by size: 6022 226 62 SalI G'TCGA_C Cuts at: 1936 3652 1936 Size: 1716 4594 SapI GCTCTTCn'nnn_ Cuts at: 2573 2743 2573 Size: 170 6140 SbfI CC_TGCA'GG Cuts at: 3662 3662 Size: 6310 ScaI AGT'ACT Cuts at: 4558 6270 4558 Size: 1712 4598 SfcI C'TryA_G Cuts at: 826 1045 2398 3658 4793 5474 5665 826 Size: 219 1353 1260 1135 681 191 1471 Fragments arranged by size: 1471 1353 1260 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2422 2422 Size: 6310 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6310 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6310 SmaI CCC'GGG Cuts at: 1872 3637 1872 Size: 1765 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2769 3262 4414 5315 5577 820 Size: 197 926 826 493 1152 901 262 1553 Fragments arranged by size: 1553 1152 926 901 826 493 262 197 SnaBI TAC'GTA Cuts at: 493 4101 493 Size: 3608 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6310 SphI G_CATG'C Cuts at: 1132 3668 1132 Size: 2536 3774 SspI AAT'ATT Cuts at: 5 52 4234 5 Size: 47 4182 2081 Fragments arranged by size: 4182 2081 47 StuI AGG'CCT Cuts at: 1517 2474 1517 Size: 957 5353 StyI C'CwwG_G Cuts at: 513 1065 2475 2745 3102 3277 3749 513 Size: 552 1410 270 357 175 472 3074 Fragments arranged by size: 3074 1410 552 472 357 270 175 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2347 4337 4354 4507 4692 5137 325 Size: 1255 767 1990 17 153 185 445 1498 Fragments arranged by size: 1990 1498 1255 767 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2083 4556 6268 Size: 274 80 33 51 1443 106 2473 1712 Cuts at: 6268 96 Size: 138 Fragments arranged by size: 2473 1712 1443 274 138 106 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2968 3000 6270 6302 2968 Size: 32 3270 32 2976 Fragments arranged by size: 3270 2976 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6310 XbaI T'CTAG_A Cuts at: 3646 3646 Size: 6310 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3174 3174 Size: 6310 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6310 XmaI C'CCGG_G Cuts at: 1870 3635 1870 Size: 1765 4545 XmnI GAAnn'nnTTC Cuts at: 2525 4439 2525 Size: 1914 4396 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4118 276 Size: 53 83 186 936 2584 2468 Fragments arranged by size: 2584 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI DraIII FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfoI SrfI SwaI