(Circular) (Six-base) MAPSORT of: pFN21AB8843.seq Check: 5722 from: 1 to: 6193 pFN21AB8843 6193 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2872 4003 278 Size: 53 83 186 936 1336 1131 2468 Fragments arranged by size: 2468 1336 1131 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2851 3536 1937 Size: 914 685 4594 Fragments arranged by size: 4594 914 685 Acc65I G'GTAC_C Cuts at: 3514 3514 Size: 6193 AclI AA'CG_TT Cuts at: 1563 4320 4693 1563 Size: 2757 373 3063 Fragments arranged by size: 3063 2757 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2811 2967 4256 5640 5908 1632 Size: 1179 156 1289 1384 268 1917 Fragments arranged by size: 1917 1384 1289 1179 268 156 AfeI AGC'GCT Cuts at: 2813 2813 Size: 6193 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5996 AflIII A'CryG_T Cuts at: 1203 5092 6069 1203 Size: 3889 977 1327 Fragments arranged by size: 3889 1327 977 AgeI A'CCGG_T Cuts at: 2846 5024 2846 Size: 2178 4015 AhdI GACnn_n'nnGTC Cuts at: 4922 4922 Size: 6193 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6193 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6193 AlwNI CAG_nnn'CTG Cuts at: 3001 3936 5508 3001 Size: 935 1572 3686 Fragments arranged by size: 3686 1572 935 ApaI G_GGCC'C Cuts at: 2149 2149 Size: 6193 ApaLI G'TGCA_C Cuts at: 4250 5406 4250 Size: 1156 5037 ApoI r'AATT_y Cuts at: 1493 2648 2825 3502 3728 3880 5989 1493 Size: 1155 177 677 226 152 2109 1697 Fragments arranged by size: 2109 1697 1155 677 226 177 152 AseI AT'TA_AT Cuts at: 160 1032 4747 160 Size: 872 3715 1606 Fragments arranged by size: 3715 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6193 AvaI C'yCGr_G Cuts at: 1870 1943 2536 3229 3518 1870 Size: 73 593 693 289 4545 Fragments arranged by size: 4545 693 593 289 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2409 2442 2409 Size: 33 6160 BamHI G'GATC_C Cuts at: 3523 3890 3523 Size: 367 5826 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2087 2273 2336 2459 3343 Size: 325 217 627 300 186 63 123 884 Cuts at: 3343 3376 3514 4969 618 Size: 33 138 1455 1842 Fragments arranged by size: 1842 1455 884 627 325 300 217 186 138 123 63 33 BanII G_rGCy'C Cuts at: 1391 2149 2751 3230 3512 1391 Size: 758 602 479 282 4072 Fragments arranged by size: 4072 758 602 479 282 BbeI G_GCGC'C Cuts at: 2091 2277 2340 3347 3380 2091 Size: 186 63 1007 33 4904 Fragments arranged by size: 4904 1007 186 63 33 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2474 928 Size: 971 575 4647 Fragments arranged by size: 4647 971 575 BbvCI CC'TCA_GC Cuts at: 2990 2990 Size: 6193 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2366 2400 4384 4418 1903 Size: 34 429 34 1984 34 3678 Fragments arranged by size: 3678 1984 429 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4085 5295 4085 Size: 1210 4983 BclI T'GATC_A Cuts at: 1548 1548 Size: 6193 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2144 2282 2408 Size: 107 122 71 631 605 472 138 126 Cuts at: 2408 3131 4804 136 Size: 723 1673 1525 Fragments arranged by size: 1673 1525 723 631 605 472 138 126 122 107 71 BglII A'GATC_T Cuts at: 6188 6188 Size: 6193 BlpI GC'TnA_GC Cuts at: 3185 3610 3185 Size: 425 5768 Bme1580I G_kGCm'C Cuts at: 1790 2149 4254 5410 1790 Size: 359 2105 1156 2573 Fragments arranged by size: 2573 2105 1156 359 BmgBI CAC'GTC Cuts at: 1318 2550 1318 Size: 1232 4961 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3924 4882 442 Size: 416 53 83 399 24 2507 958 1753 Fragments arranged by size: 2507 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6193 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2099 2216 2601 3101 3105 3236 4853 1239 Size: 860 117 385 500 4 131 1617 2579 Fragments arranged by size: 2579 1617 860 500 385 131 117 4 Bpu10I CC'TnA_GC Cuts at: 2661 2964 2990 2661 Size: 303 26 5864 Fragments arranged by size: 5864 303 26 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4318 5183 5481 4318 Size: 865 298 5030 Fragments arranged by size: 5030 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2372 2791 4856 882 Size: 617 873 419 2065 2219 Fragments arranged by size: 2219 2065 873 617 419 BsaAI yAC'GTr Cuts at: 493 1204 2095 3984 493 Size: 711 891 1889 2702 Fragments arranged by size: 2702 1889 891 711 BsaBI GATnn'nnATC Cuts at: 6187 6187 Size: 6193 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2088 2274 2337 2400 Size: 53 83 186 936 555 186 63 63 Cuts at: 2400 2869 3344 3377 3415 4000 4382 5017 275 Size: 469 475 33 38 585 382 635 1451 Fragments arranged by size: 1451 936 635 585 555 475 469 382 186 186 83 63 63 53 38 33 BsaWI w'CCGG_w Cuts at: 2846 4626 5024 5298 5445 5856 2846 Size: 1780 398 274 147 411 3183 Fragments arranged by size: 3183 1780 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2175 2205 3086 3103 3116 Size: 30 165 30 772 30 881 17 13 Cuts at: 3116 3133 3187 3217 1178 Size: 17 54 30 4154 Fragments arranged by size: 4154 881 772 165 54 30 30 30 30 17 17 13 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2598 2601 2642 2760 1186 Size: 1412 3 41 118 4619 Fragments arranged by size: 4619 1412 118 41 3 BseYI C'CCAG_C Cuts at: 3448 5396 5892 3448 Size: 1948 496 3749 Fragments arranged by size: 3749 1948 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2530 1163 Size: 573 794 4826 Fragments arranged by size: 4826 794 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2851 3269 3643 4404 5432 1540 Size: 275 186 850 418 374 761 1028 2301 Fragments arranged by size: 2301 1028 850 761 418 374 275 186 BsiHKAI G_wGCw'C Cuts at: 4254 4339 5410 4254 Size: 85 1071 5037 Fragments arranged by size: 5037 1071 85 BsmI GAATG_Cn' Cuts at: 3704 3797 3704 Size: 93 6100 BsmBI CGTCTCn'nnnn_ Cuts at: 3406 3406 Size: 6193 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2149 2751 3230 3512 4254 4339 5410 Size: 399 359 602 479 282 742 85 1071 Cuts at: 5410 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 602 479 399 359 282 85 BspEI T'CCGG_A Cuts at: 5856 5856 Size: 6193 BspHI T'CATG_A Cuts at: 4082 5812 4082 Size: 1730 4463 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3548 844 Size: 2704 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2695 3220 4080 1394 Size: 429 872 525 860 3507 Fragments arranged by size: 3507 872 860 525 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4688 4862 65 Size: 1183 3440 174 1396 Fragments arranged by size: 3440 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2846 3125 4837 5024 1700 Size: 110 1036 279 1712 187 2869 Fragments arranged by size: 2869 1712 1036 279 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6193 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6193 BssSI C'ACGA_G Cuts at: 4253 5265 4253 Size: 1012 5181 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6193 BstBI TT'CG_AA Cuts at: 4997 4997 Size: 6193 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6193 BstXI CCAn_nnnn'nTGG Cuts at: 2395 2395 Size: 6193 BstYI r'GATC_y Cuts at: 1972 3438 3523 3890 4275 4292 5744 5830 5842 Size: 1466 85 367 385 17 1452 86 12 Cuts at: 5842 6188 1972 Size: 346 1977 Fragments arranged by size: 1977 1466 1452 385 367 346 86 85 17 12 Bsu36I CC'TnA_GG Cuts at: 2455 2889 2455 Size: 434 5759 BtgI C'CryG_G Cuts at: 513 1065 2277 2302 2365 2903 513 Size: 552 1212 25 63 538 3803 Fragments arranged by size: 3803 1212 552 538 63 25 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5454 Fragments arranged by size: 5454 683 56 BtsI GCAGTG_nn' Cuts at: 2390 3709 4503 4523 2390 Size: 1319 794 20 4060 Fragments arranged by size: 4060 1319 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6193 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6158 DraI TTT'AAA Cuts at: 3497 3497 Size: 6193 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2318 5200 809 Size: 507 1002 2882 1802 Fragments arranged by size: 2882 1802 1002 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3266 3640 4529 Size: 54 1425 50 275 18 1436 374 889 Cuts at: 4529 6158 8 Size: 1629 43 Fragments arranged by size: 1629 1436 1425 889 374 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3266 3640 1537 Size: 275 1454 374 4090 Fragments arranged by size: 4090 1454 374 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2034 2619 2661 4123 1353 Size: 681 585 42 1462 3423 Fragments arranged by size: 3423 1462 681 585 42 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2924 3214 3431 4780 5154 5300 Size: 1064 138 1335 290 217 1349 374 146 Cuts at: 5300 387 Size: 1280 Fragments arranged by size: 1349 1335 1280 1064 374 290 217 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2099 2216 2601 2811 2967 3101 3105 Size: 393 467 117 385 210 156 134 4 Cuts at: 3105 3236 4256 4853 5640 5908 1239 Size: 131 1020 597 787 268 1524 Fragments arranged by size: 1524 1020 787 597 467 393 385 268 210 156 134 131 117 4 EcoO109I rG'GnC_Cy Cuts at: 2512 2755 2885 2893 2930 2512 Size: 243 130 8 37 5775 Fragments arranged by size: 5775 243 130 37 8 EcoRI G'AATT_C Cuts at: 2648 2825 3502 3880 2648 Size: 177 677 378 4961 Fragments arranged by size: 4961 677 378 177 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2077 2077 Size: 6193 FspI TGC'GCA Cuts at: 2112 4699 2112 Size: 2587 3606 HaeII r_GCGC'y Cuts at: 1678 2091 2246 2277 2340 2815 3088 3102 3347 Size: 413 155 31 63 475 273 14 245 Cuts at: 3347 3380 5340 1678 Size: 33 1960 2531 Fragments arranged by size: 2531 1960 475 413 273 245 155 63 33 31 14 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2663 2695 2790 2822 3103 Size: 32 135 32 1091 32 95 32 281 Cuts at: 3103 3135 3187 3219 3239 3271 4834 4866 4908 Size: 32 52 32 20 32 1563 32 42 Cuts at: 4908 4940 1373 Size: 32 2626 Fragments arranged by size: 2626 1563 1091 281 135 95 52 42 32 32 32 32 32 32 32 32 32 20 HincII GTy'rAC Cuts at: 669 1657 1938 2852 3111 3537 3783 669 Size: 988 281 914 259 426 246 3079 Fragments arranged by size: 3079 988 914 426 281 259 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6193 HpaI GTT'AAC Cuts at: 3783 3783 Size: 6193 KasI G'GCGC_C Cuts at: 2087 2273 2336 3343 3376 2087 Size: 186 63 1007 33 4904 Fragments arranged by size: 4904 1007 186 63 33 KpnI G_GTAC'C Cuts at: 3518 3518 Size: 6193 MfeI C'AATT_G Cuts at: 3792 3792 Size: 6193 MluI A'CGCG_T Cuts at: 6069 6069 Size: 6193 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2590 5307 5491 1305 Size: 1285 2717 184 2007 Fragments arranged by size: 2717 2007 1285 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4371 Fragments arranged by size: 4371 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4151 4510 4669 518 Size: 648 2985 359 159 2042 Fragments arranged by size: 2985 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2279 2367 2579 3609 4286 5434 5679 1606 Size: 673 88 212 1030 677 1148 245 2120 Fragments arranged by size: 2120 1148 1030 677 673 245 212 88 NaeI GCC'GGC Cuts at: 3127 3127 Size: 6193 NarI GG'CG_CC Cuts at: 2088 2274 2337 3344 3377 2088 Size: 186 63 1007 33 4904 Fragments arranged by size: 4904 1007 186 63 33 NcoI C'CATG_G Cuts at: 513 1065 2903 513 Size: 552 1838 3803 Fragments arranged by size: 3803 1838 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6193 NgoMIV G'CCGG_C Cuts at: 3125 3125 Size: 6193 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6193 NotI GC'GGCC_GC Cuts at: 3640 3640 Size: 6193 NspI r_CATG'y Cuts at: 1132 1229 3551 5096 1132 Size: 97 2322 1545 2229 Fragments arranged by size: 2322 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2501 2751 1348 Size: 1153 250 4790 Fragments arranged by size: 4790 1153 250 PciI A'CATG_T Cuts at: 5092 5092 Size: 6193 PflMI CCAn_nnn'nTGG Cuts at: 1108 2359 1108 Size: 1251 4942 PfoI T'CCnGG_A Cuts at: 2193 3243 2193 Size: 1050 5143 PmeI GTTT'AAAC Cuts at: 3497 3497 Size: 6193 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4262 4294 5775 5807 4262 Size: 32 1481 32 4648 Fragments arranged by size: 4648 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2512 2893 2930 2512 Size: 381 37 5775 Fragments arranged by size: 5775 381 37 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6193 PsiI TTA'TAA Cuts at: 3763 3763 Size: 6193 PspOMI G'GGCC_C Cuts at: 2145 2145 Size: 6193 PstI C_TGCA'G Cuts at: 830 2574 3545 830 Size: 1744 971 3478 Fragments arranged by size: 3478 1744 971 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6193 SacII CC_GC'GG Cuts at: 2280 2368 2280 Size: 88 6105 SalI G'TCGA_C Cuts at: 1936 2850 3535 1936 Size: 914 685 4594 Fragments arranged by size: 4594 914 685 SapI GCTCTTCn'nnn_ Cuts at: 2034 2034 Size: 6193 SbfI CC_TGCA'GG Cuts at: 3545 3545 Size: 6193 ScaI AGT'ACT Cuts at: 4441 6153 4441 Size: 1712 4481 SfcI C'TryA_G Cuts at: 826 1045 2570 3541 4676 5357 5548 826 Size: 219 1525 971 1135 681 191 1471 Fragments arranged by size: 1525 1471 1135 971 681 219 191 SfoI GGC'GCC Cuts at: 2089 2275 2338 3345 3378 2089 Size: 186 63 1007 33 4904 Fragments arranged by size: 4904 1007 186 63 33 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6193 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6193 SmaI CCC'GGG Cuts at: 1872 2538 3520 1872 Size: 666 982 4545 Fragments arranged by size: 4545 982 666 SmlI C'TyrA_G Cuts at: 820 1017 1943 4297 5198 5460 820 Size: 197 926 2354 901 262 1553 Fragments arranged by size: 2354 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3984 493 Size: 3491 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6193 SphI G_CATG'C Cuts at: 1132 3551 1132 Size: 2419 3774 SspI AAT'ATT Cuts at: 5 52 4117 5 Size: 47 4065 2081 Fragments arranged by size: 4065 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6193 StyI C'CwwG_G Cuts at: 513 1065 2412 2903 3632 513 Size: 552 1347 491 729 3074 Fragments arranged by size: 3074 1347 729 552 491 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2241 2800 4220 4237 4390 4575 5020 Size: 1255 661 559 1420 17 153 185 445 Cuts at: 5020 325 Size: 1498 Fragments arranged by size: 1498 1420 1255 661 559 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4439 6151 96 Size: 274 80 33 51 1443 2462 1712 138 Fragments arranged by size: 2462 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6153 6185 6153 Size: 32 6161 Tth111I GACn'n_nGTC Cuts at: 1316 2856 1316 Size: 1540 4653 XbaI T'CTAG_A Cuts at: 3529 3529 Size: 6193 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3403 3467 3403 Size: 64 6129 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6193 XmaI C'CCGG_G Cuts at: 1870 2536 3518 1870 Size: 666 982 4545 Fragments arranged by size: 4545 982 666 XmnI GAAnn'nnTTC Cuts at: 4322 4322 Size: 6193 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2870 4001 276 Size: 53 83 186 936 1336 1131 2468 Fragments arranged by size: 2468 1336 1131 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BfrBI BplI BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SanDI SexAI SfiI SrfI SwaI