(Circular) (Six-base) MAPSORT of: pFN21AB8839.seq Check: 6975 from: 1 to: 6103 pFN21AB8839 6103 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3913 278 Size: 53 83 186 936 2377 2468 Fragments arranged by size: 2468 2377 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3446 1937 Size: 1509 4594 Acc65I G'GTAC_C Cuts at: 3424 3424 Size: 6103 AclI AA'CG_TT Cuts at: 1563 4230 4603 1563 Size: 2667 373 3063 Fragments arranged by size: 3063 2667 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3036 4166 5550 5818 1632 Size: 1404 1130 1384 268 1917 Fragments arranged by size: 1917 1404 1384 1130 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5906 AflIII A'CryG_T Cuts at: 1203 5002 5979 1203 Size: 3799 977 1327 Fragments arranged by size: 3799 1327 977 AgeI A'CCGG_T Cuts at: 4934 4934 Size: 6103 AhdI GACnn_n'nnGTC Cuts at: 4832 4832 Size: 6103 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6103 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6103 AlwNI CAG_nnn'CTG Cuts at: 3846 5418 3846 Size: 1572 4531 ApaI G_GGCC'C Cuts at: 3208 3208 Size: 6103 ApaLI G'TGCA_C Cuts at: 3243 4160 5316 3243 Size: 917 1156 4030 Fragments arranged by size: 4030 1156 917 ApoI r'AATT_y Cuts at: 1493 3412 3638 3790 5899 1493 Size: 1919 226 152 2109 1697 Fragments arranged by size: 2109 1919 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4657 160 Size: 872 3625 1606 Fragments arranged by size: 3625 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6103 AvaI C'yCGr_G Cuts at: 1870 1943 2634 3144 3428 1870 Size: 73 691 510 284 4545 Fragments arranged by size: 4545 691 510 284 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2359 2392 2998 3031 2359 Size: 33 606 33 5431 Fragments arranged by size: 5431 606 33 33 BamHI G'GATC_C Cuts at: 2096 2806 3433 3800 2096 Size: 710 627 367 4399 Fragments arranged by size: 4399 710 627 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2987 3424 4879 618 Size: 325 217 627 1200 437 1455 1842 Fragments arranged by size: 1842 1455 1200 627 437 325 217 BanII G_rGCy'C Cuts at: 1391 2473 2852 3208 3422 1391 Size: 1082 379 356 214 4072 Fragments arranged by size: 4072 1082 379 356 214 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3042 3356 928 Size: 971 1143 314 3675 Fragments arranged by size: 3675 1143 971 314 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4294 4328 1903 Size: 34 2357 34 3678 Fragments arranged by size: 3678 2357 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3995 5205 3995 Size: 1210 4893 BclI T'GATC_A Cuts at: 1548 2601 1548 Size: 1053 5050 BfrBI ATG'CAT Cuts at: 2976 2976 Size: 6103 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4714 136 Size: 107 122 71 631 605 3042 1525 Fragments arranged by size: 3042 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6098 6098 Size: 6103 BlpI GC'TnA_GC Cuts at: 3520 3520 Size: 6103 Bme1580I G_kGCm'C Cuts at: 1790 3208 3247 4164 5320 1790 Size: 1418 39 917 1156 2573 Fragments arranged by size: 2573 1418 1156 917 39 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6103 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2393 3834 4792 Size: 416 53 83 399 24 976 1441 958 Cuts at: 4792 442 Size: 1753 Fragments arranged by size: 1753 1441 976 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2306 1056 Size: 1250 4853 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2150 2457 2789 4763 1239 Size: 911 307 332 1974 2579 Fragments arranged by size: 2579 1974 911 332 307 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4228 5093 5391 4228 Size: 865 298 4940 Fragments arranged by size: 4940 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4766 882 Size: 617 3267 2219 Fragments arranged by size: 3267 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3894 493 Size: 711 2690 2702 Fragments arranged by size: 2702 2690 711 BsaBI GATnn'nnATC Cuts at: 6097 6097 Size: 6103 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3910 4292 4927 275 Size: 53 83 186 936 2377 382 635 1451 Fragments arranged by size: 2377 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4536 4934 5208 5355 5766 4536 Size: 398 274 147 411 4873 Fragments arranged by size: 4873 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5878 Fragments arranged by size: 5878 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2014 2852 3126 1186 Size: 828 838 274 4163 Fragments arranged by size: 4163 838 828 274 BseYI C'CCAG_C Cuts at: 2750 5306 5802 2750 Size: 2556 496 3051 Fragments arranged by size: 3051 2556 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5530 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3553 4314 5342 1540 Size: 275 186 1552 761 1028 2301 Fragments arranged by size: 2301 1552 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2775 2852 3228 3247 4164 4249 5320 2775 Size: 77 376 19 917 85 1071 3558 Fragments arranged by size: 3558 1071 917 376 85 77 19 BsmI GAATG_Cn' Cuts at: 3614 3707 3614 Size: 93 6010 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2473 2775 2852 3208 3228 3247 3422 Size: 399 683 302 77 356 20 19 175 Cuts at: 3422 4164 4249 5320 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 683 399 356 302 175 85 77 20 19 BspEI T'CCGG_A Cuts at: 5766 5766 Size: 6103 BspHI T'CATG_A Cuts at: 2607 3992 5722 2607 Size: 1385 1730 2988 Fragments arranged by size: 2988 1730 1385 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2414 3458 844 Size: 1570 1044 3489 Fragments arranged by size: 3489 1570 1044 BsrBI CCG'CTC Cuts at: 1394 1823 3990 1394 Size: 429 2167 3507 Fragments arranged by size: 3507 2167 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2332 3340 4598 4772 65 Size: 1183 1084 1008 1258 174 1396 Fragments arranged by size: 1396 1258 1183 1084 1008 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4747 4934 1700 Size: 110 2937 187 2869 Fragments arranged by size: 2937 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2241 96 Size: 2145 3958 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6103 BssSI C'ACGA_G Cuts at: 4163 5175 4163 Size: 1012 5091 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6103 BstBI TT'CG_AA Cuts at: 4907 4907 Size: 6103 BstEII G'GTnAC_C Cuts at: 1184 2110 1184 Size: 926 5177 BstXI CCAn_nnnn'nTGG Cuts at: 2419 2938 2419 Size: 519 5584 BstYI r'GATC_y Cuts at: 1972 2096 2806 3433 3800 4185 4202 5654 5740 Size: 124 710 627 367 385 17 1452 86 Cuts at: 5740 5752 6098 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 710 627 385 367 346 124 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3033 513 Size: 552 1968 3583 Fragments arranged by size: 3583 1968 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5364 Fragments arranged by size: 5364 683 56 BtsI GCAGTG_nn' Cuts at: 2761 3619 4413 4433 2761 Size: 858 794 20 4431 Fragments arranged by size: 4431 858 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6103 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6068 DraI TTT'AAA Cuts at: 3358 3407 3358 Size: 49 6054 DraIII CAC_nnn'GTG Cuts at: 3267 3267 Size: 6103 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2318 5110 809 Size: 507 1002 2792 1802 Fragments arranged by size: 2792 1802 1002 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3550 4439 6068 Size: 54 1425 50 275 18 1720 889 1629 Cuts at: 6068 8 Size: 43 Fragments arranged by size: 1720 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3550 1537 Size: 275 1738 4090 Fragments arranged by size: 4090 1738 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2489 4033 1353 Size: 1136 1544 3423 Fragments arranged by size: 3423 1544 1136 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4690 5064 5210 387 Size: 1064 138 3101 374 146 1280 Fragments arranged by size: 3101 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2850 2850 Size: 6103 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2150 2457 2789 3036 4166 4763 5550 Size: 393 518 307 332 247 1130 597 787 Cuts at: 5550 5818 1239 Size: 268 1524 Fragments arranged by size: 1524 1130 787 597 518 393 332 307 268 247 EcoO109I rG'GnC_Cy Cuts at: 2279 2449 3205 2279 Size: 170 756 5177 Fragments arranged by size: 5177 756 170 EcoRI G'AATT_C Cuts at: 3412 3790 3412 Size: 378 5725 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3370 3370 Size: 6103 FspI TGC'GCA Cuts at: 4609 4609 Size: 6103 HaeII r_GCGC'y Cuts at: 1678 3396 5250 1678 Size: 1718 1854 2531 Fragments arranged by size: 2531 1854 1718 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2849 2881 2945 2977 4744 Size: 32 135 32 1277 32 64 32 1767 Cuts at: 4744 4776 4818 4850 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1767 1277 135 64 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2155 2176 3447 3693 669 Size: 988 281 217 21 1271 246 3079 Fragments arranged by size: 3079 1271 988 281 246 217 21 HindIII A'AGCT_T Cuts at: 748 748 Size: 6103 HpaI GTT'AAC Cuts at: 3693 3693 Size: 6103 KpnI G_GTAC'C Cuts at: 3428 3428 Size: 6103 MfeI C'AATT_G Cuts at: 3273 3702 3273 Size: 429 5674 MluI A'CGCG_T Cuts at: 5979 5979 Size: 6103 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5217 5401 1305 Size: 3912 184 2007 Fragments arranged by size: 3912 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4281 Fragments arranged by size: 4281 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2417 2612 4061 4420 4579 518 Size: 648 1251 195 1449 359 159 2042 Fragments arranged by size: 2042 1449 1251 648 359 195 159 MspA1I CmG'CkG Cuts at: 1606 2441 3519 4196 5344 5589 1606 Size: 835 1078 677 1148 245 2120 Fragments arranged by size: 2120 1148 1078 835 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5551 NdeI CA'TA_TG Cuts at: 387 387 Size: 6103 NheI G'CTAG_C Cuts at: 1052 2302 1052 Size: 1250 4853 NotI GC'GGCC_GC Cuts at: 3550 3550 Size: 6103 NsiI A_TGCA'T Cuts at: 2978 2978 Size: 6103 NspI r_CATG'y Cuts at: 1132 1229 2749 2976 3461 5006 1132 Size: 97 1520 227 485 1545 2229 Fragments arranged by size: 2229 1545 1520 485 227 97 PasI CC'CwG_GG Cuts at: 1348 2802 2938 1348 Size: 1454 136 4513 Fragments arranged by size: 4513 1454 136 PciI A'CATG_T Cuts at: 5002 5002 Size: 6103 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6103 PmeI GTTT'AAAC Cuts at: 3407 3407 Size: 6103 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4172 4204 5685 5717 4172 Size: 32 1481 32 4558 Fragments arranged by size: 4558 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2279 2449 2279 Size: 170 5933 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6103 PsiI TTA'TAA Cuts at: 2058 3381 3673 2058 Size: 1323 292 4488 Fragments arranged by size: 4488 1323 292 PspOMI G'GGCC_C Cuts at: 3204 3204 Size: 6103 PstI C_TGCA'G Cuts at: 830 3455 830 Size: 2625 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6103 PvuII CAG'CTG Cuts at: 2441 2441 Size: 6103 SacI G_AGCT'C Cuts at: 2852 2852 Size: 6103 SalI G'TCGA_C Cuts at: 1936 3445 1936 Size: 1509 4594 SbfI CC_TGCA'GG Cuts at: 3455 3455 Size: 6103 ScaI AGT'ACT Cuts at: 4351 6063 4351 Size: 1712 4391 SfcI C'TryA_G Cuts at: 826 1045 3451 4586 5267 5458 826 Size: 219 2406 1135 681 191 1471 Fragments arranged by size: 2406 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6103 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6103 SmaI CCC'GGG Cuts at: 1872 3146 3430 1872 Size: 1274 284 4545 Fragments arranged by size: 4545 1274 284 SmlI C'TyrA_G Cuts at: 820 1017 1943 4207 5108 5370 820 Size: 197 926 2264 901 262 1553 Fragments arranged by size: 2264 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3894 493 Size: 3401 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6103 SphI G_CATG'C Cuts at: 1132 3461 1132 Size: 2329 3774 SspI AAT'ATT Cuts at: 5 52 3082 4027 5 Size: 47 3030 945 2081 Fragments arranged by size: 3030 2081 945 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6103 StyI C'CwwG_G Cuts at: 513 1065 3542 513 Size: 552 2477 3074 Fragments arranged by size: 3074 2477 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4130 4147 4300 4485 4930 325 Size: 1255 2550 17 153 185 445 1498 Fragments arranged by size: 2550 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2241 4349 6061 Size: 274 80 33 51 1443 264 2108 1712 Cuts at: 6061 96 Size: 138 Fragments arranged by size: 2108 1712 1443 274 264 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2271 2303 2513 2545 2709 2741 3237 3269 6063 Size: 32 210 32 164 32 496 32 2794 Cuts at: 6063 6095 2271 Size: 32 2279 Fragments arranged by size: 2794 2279 496 210 164 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6103 XbaI T'CTAG_A Cuts at: 3439 3439 Size: 6103 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3169 3169 Size: 6103 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6103 XmaI C'CCGG_G Cuts at: 1870 3144 3428 1870 Size: 1274 284 4545 Fragments arranged by size: 4545 1274 284 XmnI GAAnn'nnTTC Cuts at: 4232 4232 Size: 6103 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3911 276 Size: 53 83 186 936 2377 2468 Fragments arranged by size: 2468 2377 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI