(Circular) (Six-base) MAPSORT of: pFN21AB8831.seq Check: 7927 from: 1 to: 6040 pFN21AB8831 6040 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2306 3850 278 Size: 53 83 186 936 770 1544 2468 Fragments arranged by size: 2468 1544 936 770 186 83 53 AccI GT'mk_AC Cuts at: 1937 3383 1937 Size: 1446 4594 Acc65I G'GTAC_C Cuts at: 3361 3361 Size: 6040 AclI AA'CG_TT Cuts at: 1563 3064 4167 4540 1563 Size: 1501 1103 373 3063 Fragments arranged by size: 3063 1501 1103 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3048 4103 5487 5755 1632 Size: 1416 1055 1384 268 1917 Fragments arranged by size: 1917 1416 1384 1055 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5843 AflIII A'CryG_T Cuts at: 1203 4939 5916 1203 Size: 3736 977 1327 Fragments arranged by size: 3736 1327 977 AgeI A'CCGG_T Cuts at: 4871 4871 Size: 6040 AhdI GACnn_n'nnGTC Cuts at: 4769 4769 Size: 6040 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6040 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6040 AlwNI CAG_nnn'CTG Cuts at: 3783 5355 3783 Size: 1572 4468 ApaLI G'TGCA_C Cuts at: 4097 5253 4097 Size: 1156 4884 ApoI r'AATT_y Cuts at: 1493 2074 3034 3349 3575 3727 5836 1493 Size: 581 960 315 226 152 2109 1697 Fragments arranged by size: 2109 1697 960 581 315 226 152 AseI AT'TA_AT Cuts at: 160 1032 4594 160 Size: 872 3562 1606 Fragments arranged by size: 3562 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6040 AvaI C'yCGr_G Cuts at: 1870 1943 3365 1870 Size: 73 1422 4545 Fragments arranged by size: 4545 1422 73 BamHI G'GATC_C Cuts at: 3370 3737 3370 Size: 367 5673 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3361 4816 618 Size: 325 217 627 1574 1455 1842 Fragments arranged by size: 1842 1574 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2019 2145 3359 1391 Size: 628 126 1214 4072 Fragments arranged by size: 4072 1214 628 126 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2153 2228 2885 3323 928 Size: 971 254 75 657 438 3645 Fragments arranged by size: 3645 971 657 438 254 75 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4231 4265 1903 Size: 34 2294 34 3678 Fragments arranged by size: 3678 2294 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3932 5142 3932 Size: 1210 4830 BclI T'GATC_A Cuts at: 1548 2766 1548 Size: 1218 4822 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4651 136 Size: 107 122 71 631 605 2979 1525 Fragments arranged by size: 2979 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3089 6035 3089 Size: 2946 3094 BlpI GC'TnA_GC Cuts at: 2579 3457 2579 Size: 878 5162 Bme1580I G_kGCm'C Cuts at: 1790 4101 5257 1790 Size: 2311 1156 2573 Fragments arranged by size: 2573 2311 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6040 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2081 3771 4729 Size: 416 53 83 399 24 664 1690 958 Cuts at: 4729 442 Size: 1753 Fragments arranged by size: 1753 1690 958 664 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6040 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2820 2944 2820 Size: 124 5916 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2886 2914 4700 1239 Size: 1647 28 1786 2579 Fragments arranged by size: 2579 1786 1647 28 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2901 4165 5030 5328 2901 Size: 1264 865 298 3613 Fragments arranged by size: 3613 1264 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4703 882 Size: 617 3204 2219 Fragments arranged by size: 3204 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3831 493 Size: 711 2627 2702 Fragments arranged by size: 2702 2627 711 BsaBI GATnn'nnATC Cuts at: 6034 6034 Size: 6040 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2303 3847 4229 4864 Size: 53 83 186 936 770 1544 382 635 Cuts at: 4864 275 Size: 1451 Fragments arranged by size: 1544 1451 936 770 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4473 4871 5145 5292 5703 4473 Size: 398 274 147 411 4810 Fragments arranged by size: 4810 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5815 Fragments arranged by size: 5815 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6040 BseYI C'CCAG_C Cuts at: 5243 5739 5243 Size: 496 5544 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5467 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3490 4251 5279 1540 Size: 275 186 1489 761 1028 2301 Fragments arranged by size: 2301 1489 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2019 4101 4186 5257 2019 Size: 2082 85 1071 2802 Fragments arranged by size: 2802 2082 1071 85 BsmI GAATG_Cn' Cuts at: 3551 3644 3551 Size: 93 5947 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2019 2145 3359 4101 4186 5257 1391 Size: 399 229 126 1214 742 85 1071 2174 Fragments arranged by size: 2174 1214 1071 742 399 229 126 85 BspEI T'CCGG_A Cuts at: 5703 5703 Size: 6040 BspHI T'CATG_A Cuts at: 2202 3046 3929 5659 2202 Size: 844 883 1730 2583 Fragments arranged by size: 2583 1730 883 844 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2577 2905 3395 844 Size: 1733 328 490 3489 Fragments arranged by size: 3489 1733 490 328 BsrBI CCG'CTC Cuts at: 1394 1823 3927 1394 Size: 429 2104 3507 Fragments arranged by size: 3507 2104 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2573 4535 4709 65 Size: 1183 1325 1962 174 1396 Fragments arranged by size: 1962 1396 1325 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4684 4871 1700 Size: 110 2874 187 2869 Fragments arranged by size: 2874 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6040 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6040 BssSI C'ACGA_G Cuts at: 4100 5112 4100 Size: 1012 5028 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6040 BstBI TT'CG_AA Cuts at: 4844 4844 Size: 6040 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6040 BstYI r'GATC_y Cuts at: 1972 3089 3370 3737 4122 4139 5591 5677 5689 Size: 1117 281 367 385 17 1452 86 12 Cuts at: 5689 6035 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1117 385 367 346 281 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2805 2805 Size: 6040 BtgI C'CryG_G Cuts at: 513 1065 2004 2467 513 Size: 552 939 463 4086 Fragments arranged by size: 4086 939 552 463 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5301 Fragments arranged by size: 5301 683 56 BtsI GCAGTG_nn' Cuts at: 2116 3556 4350 4370 2116 Size: 1440 794 20 3786 Fragments arranged by size: 3786 1440 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6040 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2779 2814 2995 3030 529 Size: 35 2215 35 181 35 3539 Fragments arranged by size: 3539 2215 181 35 35 35 DraI TTT'AAA Cuts at: 3344 3344 Size: 6040 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5047 809 Size: 507 3731 1802 Fragments arranged by size: 3731 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2609 3487 4376 Size: 54 1425 50 275 18 779 878 889 Cuts at: 4376 6005 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 878 779 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3487 1537 Size: 275 1675 4090 Fragments arranged by size: 4090 1675 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2784 2980 3970 1353 Size: 1431 196 990 3423 Fragments arranged by size: 3423 1431 990 196 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4627 5001 5147 387 Size: 1064 138 3038 374 146 1280 Fragments arranged by size: 3038 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2017 2017 Size: 6040 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2886 2914 3048 4103 4700 5487 5755 Size: 393 1254 28 134 1055 597 787 268 Cuts at: 5755 1239 Size: 1524 Fragments arranged by size: 1524 1254 1055 787 597 393 268 134 28 EcoO109I rG'GnC_Cy Cuts at: 3001 3001 Size: 6040 EcoRI G'AATT_C Cuts at: 2074 3349 3727 2074 Size: 1275 378 4387 Fragments arranged by size: 4387 1275 378 EcoRV GAT'ATC Cuts at: 2266 2266 Size: 6040 FspI TGC'GCA Cuts at: 4546 4546 Size: 6040 HaeII r_GCGC'y Cuts at: 1678 5187 1678 Size: 3509 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2422 2454 3299 3331 4681 Size: 32 135 32 850 32 845 32 1350 Cuts at: 4681 4713 4755 4787 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1350 850 845 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3384 3630 669 Size: 988 281 1446 246 3079 Fragments arranged by size: 3079 1446 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6040 HpaI GTT'AAC Cuts at: 3630 3630 Size: 6040 KpnI G_GTAC'C Cuts at: 3365 3365 Size: 6040 MfeI C'AATT_G Cuts at: 2406 3639 2406 Size: 1233 4807 MluI A'CGCG_T Cuts at: 5916 5916 Size: 6040 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2757 3125 5154 5338 1305 Size: 1452 368 2029 184 2007 Fragments arranged by size: 2029 2007 1452 368 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2611 10 Size: 54 1425 343 779 3439 Fragments arranged by size: 3439 1425 779 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2700 3998 4357 4516 518 Size: 648 1534 1298 359 159 2042 Fragments arranged by size: 2042 1534 1298 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3456 4133 5281 5526 1606 Size: 1850 677 1148 245 2120 Fragments arranged by size: 2120 1850 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2467 513 Size: 552 939 463 4086 Fragments arranged by size: 4086 939 552 463 NdeI CA'TA_TG Cuts at: 387 2427 3336 387 Size: 2040 909 3091 Fragments arranged by size: 3091 2040 909 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6040 NotI GC'GGCC_GC Cuts at: 3487 3487 Size: 6040 NspI r_CATG'y Cuts at: 1132 1229 2889 3398 4943 1132 Size: 97 1660 509 1545 2229 Fragments arranged by size: 2229 1660 1545 509 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6040 PciI A'CATG_T Cuts at: 4939 4939 Size: 6040 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6040 PmeI GTTT'AAAC Cuts at: 3344 3344 Size: 6040 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4109 4141 5622 5654 4109 Size: 32 1481 32 4495 Fragments arranged by size: 4495 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3001 3001 Size: 6040 PshAI GACnn'nnGTC Cuts at: 1613 2306 1613 Size: 693 5347 PsiI TTA'TAA Cuts at: 2031 3610 2031 Size: 1579 4461 PstI C_TGCA'G Cuts at: 830 2711 3392 830 Size: 1881 681 3478 Fragments arranged by size: 3478 1881 681 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6040 SacI G_AGCT'C Cuts at: 2019 2019 Size: 6040 SalI G'TCGA_C Cuts at: 1936 3382 1936 Size: 1446 4594 SanDI GG'GwC_CC Cuts at: 3001 3001 Size: 6040 SbfI CC_TGCA'GG Cuts at: 3392 3392 Size: 6040 ScaI AGT'ACT Cuts at: 4288 6000 4288 Size: 1712 4328 SfcI C'TryA_G Cuts at: 826 1045 2707 3106 3388 4523 5204 5395 826 Size: 219 1662 399 282 1135 681 191 1471 Fragments arranged by size: 1662 1471 1135 681 399 282 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6040 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6040 SmaI CCC'GGG Cuts at: 1872 3367 1872 Size: 1495 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2916 4144 5045 5307 820 Size: 197 926 973 1228 901 262 1553 Fragments arranged by size: 1553 1228 973 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3831 493 Size: 3338 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6040 SphI G_CATG'C Cuts at: 1132 3398 1132 Size: 2266 3774 SspI AAT'ATT Cuts at: 5 52 3964 5 Size: 47 3912 2081 Fragments arranged by size: 3912 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6040 StyI C'CwwG_G Cuts at: 513 1065 2004 2467 3479 513 Size: 552 939 463 1012 3074 Fragments arranged by size: 3074 1012 939 552 463 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4067 4084 4237 4422 4867 325 Size: 1255 2487 17 153 185 445 1498 Fragments arranged by size: 2487 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4286 5998 96 Size: 274 80 33 51 1443 2309 1712 138 Fragments arranged by size: 2309 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6000 6032 6000 Size: 32 6008 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6040 XbaI T'CTAG_A Cuts at: 3376 3376 Size: 6040 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6040 XmaI C'CCGG_G Cuts at: 1870 3365 1870 Size: 1495 4545 XmnI GAAnn'nnTTC Cuts at: 4169 4169 Size: 6040 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2304 3848 276 Size: 53 83 186 936 770 1544 2468 Fragments arranged by size: 2468 1544 936 770 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI