(Linear) (Six-base) MAPSORT of: KIBB8831.seq  Check: 3924  from: 1  to: 1335



KIBB8831.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:08  ..

AatII G_ACGT'C

Cuts at:      0     301    1335 
   Size:        301    1034
 
AclI AA'CG_TT

Cuts at:      0    1059    1335 
   Size:       1059     276
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1043    1335 
   Size:       1043     292
 
ApoI r'AATT_y

Cuts at:      0      69    1029    1335 
   Size:         69     960     306

  Fragments arranged by size: 

                 960     306      69
 
BanII G_rGCy'C

Cuts at:      0      14     140    1335 
   Size:         14     126    1195

  Fragments arranged by size: 

                1195     126      14
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     148     223     880    1318    1335 
   Size:        148      75     657     438      17

  Fragments arranged by size: 

                 657     438     148      75      17
 
BclI T'GATC_A

Cuts at:      0     761    1335 
   Size:        761     574
 
BglII A'GATC_T

Cuts at:      0    1084    1335 
   Size:       1084     251
 
BlpI GC'TnA_GC

Cuts at:      0     574    1335 
   Size:        574     761
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      76    1335 
   Size:         76    1259
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     815     939    1335 
   Size:        815     124     396

  Fragments arranged by size: 

                 815     396     124
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     881     909    1335 
   Size:        881      28     426

  Fragments arranged by size: 

                 881     426      28
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     896    1335 
   Size:        896     439
 
BsaHI Gr'CG_yC

Cuts at:      0     298    1335 
   Size:        298    1037
 
BsiHKAI G_wGCw'C

Cuts at:      0      14    1335 
   Size:         14    1321
 
Bsp1286I G_dGCh'C

Cuts at:      0      14     140    1335 
   Size:         14     126    1195

  Fragments arranged by size: 

                1195     126      14
 
BspHI T'CATG_A

Cuts at:      0     197    1041    1335 
   Size:        197     844     294

  Fragments arranged by size: 

                 844     294     197
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     572     900    1335 
   Size:        572     328     435

  Fragments arranged by size: 

                 572     435     328
 
BsrDI GCAATG_nn'

Cuts at:      0     568    1335 
   Size:        568     767
 
BstYI r'GATC_y

Cuts at:      0    1084    1335 
   Size:       1084     251
 
Bsu36I CC'TnA_GG

Cuts at:      0     800    1335 
   Size:        800     535
 
BtgI C'CryG_G

Cuts at:      0     462    1335 
   Size:        462     873
 
BtsI GCAGTG_nn'

Cuts at:      0     111    1335 
   Size:        111    1224
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     774     809     990    1025    1335 
   Size:        774      35     181      35     310

  Fragments arranged by size: 

                 774     310     181      35      35
 
EaeI y'GGCC_r

Cuts at:      0     604    1335 
   Size:        604     731
 
EarI CTCTTCn'nnn_

Cuts at:      0     779     975    1335 
   Size:        779     196     360

  Fragments arranged by size: 

                 779     360     196
 
EcoICRI GAG'CTC

Cuts at:      0      12    1335 
   Size:         12    1323
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     881     909    1043    1335 
   Size:        881      28     134     292

  Fragments arranged by size: 

                 881     292     134      28
 
EcoO109I rG'GnC_Cy

Cuts at:      0     996    1335 
   Size:        996     339
 
EcoRI G'AATT_C

Cuts at:      0      69    1335 
   Size:         69    1266
 
EcoRV GAT'ATC

Cuts at:      0     261    1335 
   Size:        261    1074
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     417     449    1294    1326    1335 
   Size:        417      32     845      32       9

  Fragments arranged by size: 

                 845     417      32      32       9
 
MfeI C'AATT_G

Cuts at:      0     401    1335 
   Size:        401     934
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     752    1120    1335 
   Size:        752     368     215

  Fragments arranged by size: 

                 752     368     215
 
MscI TGG'CCA

Cuts at:      0     606    1335 
   Size:        606     729
 
MslI CAynn'nnrTG

Cuts at:      0     695    1335 
   Size:        695     640
 
NcoI C'CATG_G

Cuts at:      0     462    1335 
   Size:        462     873
 
NdeI CA'TA_TG

Cuts at:      0     422    1331    1335 
   Size:        422     909       4

  Fragments arranged by size: 

                 909     422       4
 
NspI r_CATG'y

Cuts at:      0     884    1335 
   Size:        884     451
 
PpuMI rG'GwC_Cy

Cuts at:      0     996    1335 
   Size:        996     339
 
PshAI GACnn'nnGTC

Cuts at:      0     301    1335 
   Size:        301    1034
 
PsiI TTA'TAA

Cuts at:      0      26    1335 
   Size:         26    1309
 
PstI C_TGCA'G

Cuts at:      0     706    1335 
   Size:        706     629
 
SacI G_AGCT'C

Cuts at:      0      14    1335 
   Size:         14    1321
 
SanDI GG'GwC_CC

Cuts at:      0     996    1335 
   Size:        996     339
 
SfcI C'TryA_G

Cuts at:      0     702    1101    1335 
   Size:        702     399     234

  Fragments arranged by size: 

                 702     399     234
 
SmlI C'TyrA_G

Cuts at:      0     911    1335 
   Size:        911     424
 
StyI C'CwwG_G

Cuts at:      0     462    1335 
   Size:        462     873
 
ZraI GAC'GTC

Cuts at:      0     299    1335 
   Size:        299    1036
 

 Enzymes that do cut:

    AatII     AclI     AcuI     ApoI    BanII     BbsI     BclI    BglII
     BlpI     BmrI     BplI     BpmI    BpuEI    BsaHI  BsiHKAI Bsp1286I
    BspHI    BspMI    BsrDI    BstYI   Bsu36I     BtgI     BtsI    CspCI
     EaeI     EarI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV    Hin4I
     MfeI     MmeI     MscI     MslI     NcoI     NdeI     NspI    PpuMI
    PshAI     PsiI     PstI     SacI    SanDI     SfcI     SmlI     StyI
     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI
     BcgI    BciVI    BfrBI     BglI Bme1580I    BmgBI     BmtI   Bpu10I
     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I    BtgZI
     ClaI     DraI   DraIII     DrdI     EagI     EciI    EcoNI     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MluI   MspA1I     NaeI     NarI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
    SacII     SalI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI