Restriction map for
pFN21AB8830
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# NO CUT ENZYMES ( 169 enzymes) #
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AatII AccI AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmFI
BsmI BsoBI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstYI BstZ17I Bsu36I Cac8I
CelII Cfr10I CpoI DraI DraIII
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HapII Hin1I HincII
HindII HindIII HpaI HpaII HphI
KasI KspI MaeII MamI MfeI
MflI MluI MluNI MroI MscI
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SphI Sse8387I StuI StyI
SwaI Tsp45I Tth111I Van91I XbaI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 34 enzymes) #
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Acc65I 916 GGTACC G^GTACC
Asp700I 1120 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 916 GGTACC G^GTACC
BanI 916 GGTACC G^G[CT][GA]CC
BanII 1075 GGGCTC G[GA]GC[CT]^C
BmyI 1075 GGGCTC G[AGT]GC[ACT]^C
BsaJI 293 CCGTGG C^C[ACGT][ACGT]GG
Bsp1286I 1075 GGGCTC G[AGT]GC[ACT]^C
Bsp1407I 1198 TGTACA T^GTACA
BsrDI 1314 GCAATG GCAATG(2/0)
BsrGI 1198 TGTACA T^GTACA
BssKI 648 CCTGG ^CC[ACGT]GG
BstNI 648 CCTGG CC^[AT]GG
CfoI 1103 GCGC GCG^C
DsaI 293 CCGTGG C^C[GA][CT]GG
EcoRII 648 CCTGG ^CC[AT]GG
HaeII 1102 AGCGCC [GA]GCGC^[CT]
HgaI 974 GACGC GACGC(5/10)
HhaI 1103 GCGC GCG^C
HinP1I 1103 GCGC G^CGC
KpnI 916 GGTACC GGTAC^C
MspA1I 207 CAGCTG C[AC]G^C[GT]G
MvaI 648 CCTGG CC^[AT]GG
MwoI 883 GCAGCCGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PleI 934 GACTC GAGTC(4/5)
PvuII 207 CAGCTG CAG^CTG
ScaI 1287 AGTACT AGT^ACT
ScrFI 648 CCTGG CC^[ACGT]GG
SexAI 647 ACCTGGT A^CC[AT]GGT
SpeI 52 ACTAGT A^CTAGT
SspBI 1198 TGTACA T^GTACA
SspI 19 AATATT AAT^ATT
XcmI 740 CCAAATGGGATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1120 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AlwNI 140 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
277 CAGAAACTG
AvaII 622 GGTCC G^G[AT]CC
1095 GGACC
BbsI 946 GAAGAC GAAGAC(2/6)
1051 GTCTTC
BbvI 883 GCAGC GCAGC(8/12)
1167 GCAGC
BpuAI 946 GAAGAC GAAGAC(2/6)
1051 GTCTTC
BspDI 251 ATCGAT AT^CGAT
1216 ATCGAT
BstXI 218 CCATTGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
853 CCATGTTACTGG
ClaI 251 ATCGAT AT^CGAT
1216 ATCGAT
DraII 621 AGGTCCT [GA]G^G[ACGT]CC[CT]
1094 AGGACCC
DrdI 974 GACGCTATAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1042 GACCCCTGTGTC
EarI 336 CTCTTC CTCTTC(1/4)
989 CTCTTC
EcoO109I 621 AGGTCCT [GA]G^G[ACGT]CC[CT]
1094 AGGACCC
Fnu4HI 883 GCAGC GC^[ACGT]GC
1167 GCAGC
HaeIII 44 GGCC GG^CC
262 GGCC
ItaI 883 GCAGC GC^[ACGT]GC
1167 GCAGC
Ksp632I 336 CTCTTC CTCTTC(1/4)
989 CTCTTC
MaeIII 857 GTTAC ^GT[ACGT]AC
1193 GTAAC
PpuMI 621 AGGTCCT [GA]G^G[AT]CC[CT]
1094 AGGACCC
PstI 766 CTGCAG CTGCA^G
1165 CTGCAG
TfiI 685 GATTC G^A[AT]TC
1014 GAATC
TseI 883 GCAGC G^C[AT]GC
1167 GCAGC
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# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AcsI AfaI AluI ApoI BfaI
BsiYI BslI BsmAI BsrI BstF5I
Cfr13I DdeI DpnII FokI HinfI
MaeI MboI MboII MnlI MseI
MslI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I SfaNI SfcI TaqI
Tru9I Tsp509I TspRI TthHB8I