(Circular) (Six-base) MAPSORT of: pFN21AB8825.seq Check: 3589 from: 1 to: 5950 pFN21AB8825 5950 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2516 2666 2516 Size: 150 5800 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3760 278 Size: 53 83 186 936 2224 2468 Fragments arranged by size: 2468 2224 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3033 3293 1937 Size: 1096 260 4594 Fragments arranged by size: 4594 1096 260 Acc65I G'GTAC_C Cuts at: 3271 3271 Size: 5950 AclI AA'CG_TT Cuts at: 1563 4077 4450 1563 Size: 2514 373 3063 Fragments arranged by size: 3063 2514 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2197 2269 4013 5397 5665 1632 Size: 565 72 1744 1384 268 1917 Fragments arranged by size: 1917 1744 1384 565 268 72 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5753 AflIII A'CryG_T Cuts at: 1203 4849 5826 1203 Size: 3646 977 1327 Fragments arranged by size: 3646 1327 977 AgeI A'CCGG_T Cuts at: 4781 4781 Size: 5950 AhdI GACnn_n'nnGTC Cuts at: 4679 4679 Size: 5950 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5950 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2804 1184 Size: 1620 4330 AlwNI CAG_nnn'CTG Cuts at: 3693 5265 3693 Size: 1572 4378 ApaI G_GGCC'C Cuts at: 2637 2637 Size: 5950 ApaLI G'TGCA_C Cuts at: 4007 5163 4007 Size: 1156 4794 ApoI r'AATT_y Cuts at: 1493 3259 3485 3637 5746 1493 Size: 1766 226 152 2109 1697 Fragments arranged by size: 2109 1766 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4504 160 Size: 872 3472 1606 Fragments arranged by size: 3472 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5950 AvaI C'yCGr_G Cuts at: 1870 1943 3241 3275 1870 Size: 73 1298 34 4545 Fragments arranged by size: 4545 1298 73 34 BamHI G'GATC_C Cuts at: 3280 3647 3280 Size: 367 5583 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2528 2892 3271 4726 618 Size: 325 217 627 741 364 379 1455 1842 Fragments arranged by size: 1842 1455 741 627 379 364 325 217 BanII G_rGCy'C Cuts at: 1391 2563 2637 2679 3177 3269 1391 Size: 1172 74 42 498 92 4072 Fragments arranged by size: 4072 1172 498 92 74 42 BbeI G_GCGC'C Cuts at: 2896 2896 Size: 5950 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2038 3186 928 Size: 971 139 1148 3692 Fragments arranged by size: 3692 1148 971 139 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4141 4175 1903 Size: 34 2204 34 3678 Fragments arranged by size: 3678 2204 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3842 5052 3842 Size: 1210 4740 BclI T'GATC_A Cuts at: 1548 1548 Size: 5950 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4561 136 Size: 107 122 71 631 605 2889 1525 Fragments arranged by size: 2889 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5945 5945 Size: 5950 BlpI GC'TnA_GC Cuts at: 2381 3367 2381 Size: 986 4964 Bme1580I G_kGCm'C Cuts at: 1790 2533 2637 4011 5167 1790 Size: 743 104 1374 1156 2573 Fragments arranged by size: 2573 1374 1156 743 104 BmgBI CAC'GTC Cuts at: 1318 3037 1318 Size: 1719 4231 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3681 4639 442 Size: 416 53 83 399 24 2264 958 1753 Fragments arranged by size: 2264 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5950 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2266 2266 Size: 5950 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2225 3161 4610 1239 Size: 986 936 1449 2579 Fragments arranged by size: 2579 1449 986 936 Bpu10I CC'TnA_GC Cuts at: 2399 2399 Size: 5950 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4075 4940 5238 4075 Size: 865 298 4787 Fragments arranged by size: 4787 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3215 4613 882 Size: 617 1716 1398 2219 Fragments arranged by size: 2219 1716 1398 617 BsaAI yAC'GTr Cuts at: 493 1204 3741 493 Size: 711 2537 2702 Fragments arranged by size: 2702 2537 711 BsaBI GATnn'nnATC Cuts at: 5944 5944 Size: 5950 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2184 2893 3757 4139 Size: 53 83 186 936 651 709 864 382 Cuts at: 4139 4774 275 Size: 635 1451 Fragments arranged by size: 1451 936 864 709 651 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4383 4781 5055 5202 5613 4383 Size: 398 274 147 411 4720 Fragments arranged by size: 4720 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3116 3146 1178 Size: 30 165 30 1713 30 3982 Fragments arranged by size: 3982 1713 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2070 2321 1186 Size: 884 251 4815 Fragments arranged by size: 4815 884 251 BseYI C'CCAG_C Cuts at: 2341 2350 2616 5153 5649 2341 Size: 9 266 2537 496 2642 Fragments arranged by size: 2642 2537 496 266 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5377 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2302 3400 4161 5189 1540 Size: 275 186 301 1098 761 1028 2301 Fragments arranged by size: 2301 1098 1028 761 301 275 186 BsiHKAI G_wGCw'C Cuts at: 4011 4096 5167 4011 Size: 85 1071 4794 Fragments arranged by size: 4794 1071 85 BsmI GAATG_Cn' Cuts at: 3461 3554 3461 Size: 93 5857 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2533 2563 2637 2679 3177 3269 4011 Size: 399 743 30 74 42 498 92 742 Cuts at: 4011 4096 5167 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 743 742 498 399 92 85 74 42 30 BspEI T'CCGG_A Cuts at: 5613 5613 Size: 5950 BspHI T'CATG_A Cuts at: 3839 5569 3839 Size: 1730 4220 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2516 2666 3305 844 Size: 1672 150 639 3489 Fragments arranged by size: 3489 1672 639 150 BsrBI CCG'CTC Cuts at: 1394 1823 2467 3127 3837 1394 Size: 429 644 660 710 3507 Fragments arranged by size: 3507 710 660 644 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4445 4619 65 Size: 1183 3197 174 1396 Fragments arranged by size: 3197 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2385 4594 4781 1700 Size: 110 575 2209 187 2869 Fragments arranged by size: 2869 2209 575 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5950 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5950 BssSI C'ACGA_G Cuts at: 2251 4010 5022 2251 Size: 1759 1012 3179 Fragments arranged by size: 3179 1759 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5950 BstBI TT'CG_AA Cuts at: 4754 4754 Size: 5950 BstEII G'GTnAC_C Cuts at: 1184 3049 1184 Size: 1865 4085 BstXI CCAn_nnnn'nTGG Cuts at: 2911 2911 Size: 5950 BstYI r'GATC_y Cuts at: 1972 3280 3647 4032 4049 5501 5587 5599 5945 Size: 1308 367 385 17 1452 86 12 346 Cuts at: 5945 1972 Size: 1977 Fragments arranged by size: 1977 1452 1308 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3055 3055 Size: 5950 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4459 Fragments arranged by size: 4459 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5211 Fragments arranged by size: 5211 683 56 BtsI GCAGTG_nn' Cuts at: 2459 2481 2749 3073 3466 4260 4280 2459 Size: 22 268 324 393 794 20 4129 Fragments arranged by size: 4129 794 393 324 268 22 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5950 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5915 DraI TTT'AAA Cuts at: 3254 3254 Size: 5950 DraIII CAC_nnn'GTG Cuts at: 2173 2965 2173 Size: 792 5158 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2486 4957 809 Size: 507 1170 2471 1802 Fragments arranged by size: 2471 1802 1170 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2862 3016 3039 Size: 54 1425 50 275 18 1032 154 23 Cuts at: 3039 3397 4286 5915 8 Size: 358 889 1629 43 Fragments arranged by size: 1629 1425 1032 889 358 275 154 54 50 43 23 18 EagI C'GGCC_G Cuts at: 1537 1812 3397 1537 Size: 275 1585 4090 Fragments arranged by size: 4090 1585 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2224 2540 3880 1353 Size: 871 316 1340 3423 Fragments arranged by size: 3423 1340 871 316 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2404 4537 4911 5057 387 Size: 1064 138 815 2133 374 146 1280 Fragments arranged by size: 2133 1280 1064 815 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2197 2225 2269 3161 4013 4610 5397 Size: 393 565 28 44 892 852 597 787 Cuts at: 5397 5665 1239 Size: 268 1524 Fragments arranged by size: 1524 892 852 787 597 565 393 268 44 28 EcoO109I rG'GnC_Cy Cuts at: 2633 2634 2633 Size: 1 5949 EcoRI G'AATT_C Cuts at: 3259 3637 3259 Size: 378 5572 FspI TGC'GCA Cuts at: 4456 4456 Size: 5950 HaeII r_GCGC'y Cuts at: 1678 2737 2896 3000 5097 1678 Size: 1059 159 104 2097 2531 Fragments arranged by size: 2531 2097 1059 159 104 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2941 2973 4591 4623 4665 Size: 32 135 32 1369 32 1618 32 42 Cuts at: 4665 4697 1373 Size: 32 2626 Fragments arranged by size: 2626 1618 1369 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3294 3540 669 Size: 988 281 1356 246 3079 Fragments arranged by size: 3079 1356 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5950 HpaI GTT'AAC Cuts at: 3540 3540 Size: 5950 KasI G'GCGC_C Cuts at: 2892 2892 Size: 5950 KpnI G_GTAC'C Cuts at: 3275 3275 Size: 5950 MfeI C'AATT_G Cuts at: 3549 3549 Size: 5950 MluI A'CGCG_T Cuts at: 5826 5826 Size: 5950 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5064 5248 1305 Size: 3759 184 2007 Fragments arranged by size: 3759 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3041 10 Size: 54 1425 343 1209 2919 Fragments arranged by size: 2919 1425 1209 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2984 3908 4267 4426 518 Size: 648 1818 924 359 159 2042 Fragments arranged by size: 2042 1818 924 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2803 2890 3091 3366 4043 5191 5436 1606 Size: 1197 87 201 275 677 1148 245 2120 Fragments arranged by size: 2120 1197 1148 677 275 245 201 87 NaeI GCC'GGC Cuts at: 2387 2387 Size: 5950 NarI GG'CG_CC Cuts at: 2893 2893 Size: 5950 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4459 Fragments arranged by size: 4459 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5950 NgoMIV G'CCGG_C Cuts at: 2385 2385 Size: 5950 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5950 NotI GC'GGCC_GC Cuts at: 3397 3397 Size: 5950 NspI r_CATG'y Cuts at: 1132 1229 3073 3308 4853 1132 Size: 97 1844 235 1545 2229 Fragments arranged by size: 2229 1844 1545 235 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5950 PciI A'CATG_T Cuts at: 4849 4849 Size: 5950 PflMI CCAn_nnn'nTGG Cuts at: 1108 2860 1108 Size: 1752 4198 PfoI T'CCnGG_A Cuts at: 2202 3120 2202 Size: 918 5032 PmeI GTTT'AAAC Cuts at: 3254 3254 Size: 5950 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2201 2233 4019 4051 5532 5564 2201 Size: 32 1786 32 1481 32 2587 Fragments arranged by size: 2587 1786 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5950 PsiI TTA'TAA Cuts at: 3520 3520 Size: 5950 PspOMI G'GGCC_C Cuts at: 2633 2633 Size: 5950 PstI C_TGCA'G Cuts at: 830 2802 3302 830 Size: 1972 500 3478 Fragments arranged by size: 3478 1972 500 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5950 PvuII CAG'CTG Cuts at: 2803 2803 Size: 5950 SalI G'TCGA_C Cuts at: 1936 3292 1936 Size: 1356 4594 SapI GCTCTTCn'nnn_ Cuts at: 2224 2224 Size: 5950 SbfI CC_TGCA'GG Cuts at: 3302 3302 Size: 5950 ScaI AGT'ACT Cuts at: 4198 5910 4198 Size: 1712 4238 SexAI A'CCwGG_T Cuts at: 2961 2961 Size: 5950 SfcI C'TryA_G Cuts at: 826 1045 2323 2433 2798 3298 4433 5114 5305 Size: 219 1278 110 365 500 1135 681 191 Cuts at: 5305 826 Size: 1471 Fragments arranged by size: 1471 1278 1135 681 500 365 219 191 110 SfoI GGC'GCC Cuts at: 2894 2894 Size: 5950 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5950 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5950 SmaI CCC'GGG Cuts at: 1872 3277 1872 Size: 1405 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4054 4955 5217 820 Size: 197 926 2111 901 262 1553 Fragments arranged by size: 2111 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3741 493 Size: 3248 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5950 SphI G_CATG'C Cuts at: 1132 3308 1132 Size: 2176 3774 SspI AAT'ATT Cuts at: 5 52 3874 5 Size: 47 3822 2081 Fragments arranged by size: 3822 2081 47 StuI AGG'CCT Cuts at: 1517 2118 3060 3233 1517 Size: 601 942 173 4234 Fragments arranged by size: 4234 942 601 173 StyI C'CwwG_G Cuts at: 513 1065 2004 3389 513 Size: 552 939 1385 3074 Fragments arranged by size: 3074 1385 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2630 2762 3037 3977 3994 4147 4332 Size: 1255 1050 132 275 940 17 153 185 Cuts at: 4332 4777 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1050 940 445 275 185 153 132 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4196 5908 96 Size: 274 80 33 51 1443 2219 1712 138 Fragments arranged by size: 2219 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3116 3148 5910 5942 3116 Size: 32 2762 32 3124 Fragments arranged by size: 3124 2762 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5950 XbaI T'CTAG_A Cuts at: 3286 3286 Size: 5950 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2498 2911 2498 Size: 413 5537 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5950 XmaI C'CCGG_G Cuts at: 1870 3275 1870 Size: 1405 4545 XmnI GAAnn'nnTTC Cuts at: 4079 4079 Size: 5950 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3758 276 Size: 53 83 186 936 2224 2468 Fragments arranged by size: 2468 2224 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SfiI SrfI SwaI