Restriction map for
pFN21AB8825
(Linear) (Six-base) MAPSORT of: KIBB8825.seq Check: 2373 from: 1 to: 1245
KIBB8825.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 511 661 1245
Size: 511 150 584
Fragments arranged by size:
584 511 150
AccI GT'mk_AC
Cuts at: 0 1028 1245
Size: 1028 217
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 192 264 1245
Size: 192 72 981
Fragments arranged by size:
981 192 72
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 799 1245
Size: 799 446
ApaI G_GGCC'C
Cuts at: 0 632 1245
Size: 632 613
AvaI C'yCGr_G
Cuts at: 0 1236 1245
Size: 1236 9
BanI G'GyrC_C
Cuts at: 0 523 887 1245
Size: 523 364 358
Fragments arranged by size:
523 364 358
BanII G_rGCy'C
Cuts at: 0 558 632 674 1172 1245
Size: 558 74 42 498 73
Fragments arranged by size:
558 498 74 73 42
BbeI G_GCGC'C
Cuts at: 0 891 1245
Size: 891 354
BbsI GAAGACnn'nnnn_
Cuts at: 0 33 1181 1245
Size: 33 1148 64
Fragments arranged by size:
1148 64 33
BlpI GC'TnA_GC
Cuts at: 0 376 1245
Size: 376 869
Bme1580I G_kGCm'C
Cuts at: 0 528 632 1245
Size: 528 104 613
Fragments arranged by size:
613 528 104
BmgBI CAC'GTC
Cuts at: 0 1032 1245
Size: 1032 213
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 261 1245
Size: 261 984
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 1156 1245
Size: 220 936 89
Fragments arranged by size:
936 220 89
Bpu10I CC'TnA_GC
Cuts at: 0 394 1245
Size: 394 851
BsaI GGTCTCn'nnnn_
Cuts at: 0 1210 1245
Size: 1210 35
BsaHI Gr'CG_yC
Cuts at: 0 179 888 1245
Size: 179 709 357
Fragments arranged by size:
709 357 179
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1111 1141 1245
Size: 1111 30 104
Fragments arranged by size:
1111 104 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 65 316 1245
Size: 65 251 929
Fragments arranged by size:
929 251 65
BseYI C'CCAG_C
Cuts at: 0 336 345 611 1245
Size: 336 9 266 634
Fragments arranged by size:
634 336 266 9
BsiEI CG_ry'CG
Cuts at: 0 297 1245
Size: 297 948
Bsp1286I G_dGCh'C
Cuts at: 0 528 558 632 674 1172 1245
Size: 528 30 74 42 498 73
Fragments arranged by size:
528 498 74 73 42 30
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 511 661 1245
Size: 511 150 584
Fragments arranged by size:
584 511 150
BsrBI CCG'CTC
Cuts at: 0 462 1122 1245
Size: 462 660 123
Fragments arranged by size:
660 462 123
BsrFI r'CCGG_y
Cuts at: 0 380 1245
Size: 380 865
BssSI C'ACGA_G
Cuts at: 0 246 1245
Size: 246 999
BstEII G'GTnAC_C
Cuts at: 0 1044 1245
Size: 1044 201
BstXI CCAn_nnnn'nTGG
Cuts at: 0 906 1245
Size: 906 339
Bsu36I CC'TnA_GG
Cuts at: 0 1050 1245
Size: 1050 195
BtsI GCAGTG_nn'
Cuts at: 0 454 476 744 1068 1245
Size: 454 22 268 324 177
Fragments arranged by size:
454 324 268 177 22
DraIII CAC_nnn'GTG
Cuts at: 0 168 960 1245
Size: 168 792 285
Fragments arranged by size:
792 285 168
DrdI GACnn_nn'nnGTC
Cuts at: 0 481 1245
Size: 481 764
EaeI y'GGCC_r
Cuts at: 0 857 1011 1034 1245
Size: 857 154 23 211
Fragments arranged by size:
857 211 154 23
EarI CTCTTCn'nnn_
Cuts at: 0 219 535 1245
Size: 219 316 710
Fragments arranged by size:
710 316 219
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 399 1245
Size: 399 846
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 192 220 264 1156 1245
Size: 192 28 44 892 89
Fragments arranged by size:
892 192 89 44 28
EcoO109I rG'GnC_Cy
Cuts at: 0 628 629 1245
Size: 628 1 616
Fragments arranged by size:
628 616 1
HaeII r_GCGC'y
Cuts at: 0 732 891 995 1245
Size: 732 159 104 250
Fragments arranged by size:
732 250 159 104
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 936 968 1245
Size: 936 32 277
Fragments arranged by size:
936 277 32
KasI G'GCGC_C
Cuts at: 0 887 1245
Size: 887 358
MscI TGG'CCA
Cuts at: 0 1036 1245
Size: 1036 209
MslI CAynn'nnrTG
Cuts at: 0 979 1245
Size: 979 266
MspA1I CmG'CkG
Cuts at: 0 798 885 1086 1245
Size: 798 87 201 159
Fragments arranged by size:
798 201 159 87
NaeI GCC'GGC
Cuts at: 0 382 1245
Size: 382 863
NarI GG'CG_CC
Cuts at: 0 888 1245
Size: 888 357
NgoMIV G'CCGG_C
Cuts at: 0 380 1245
Size: 380 865
NspI r_CATG'y
Cuts at: 0 1068 1245
Size: 1068 177
PflMI CCAn_nnn'nTGG
Cuts at: 0 855 1245
Size: 855 390
PfoI T'CCnGG_A
Cuts at: 0 197 1115 1245
Size: 197 918 130
Fragments arranged by size:
918 197 130
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 196 228 1245
Size: 196 32 1017
Fragments arranged by size:
1017 196 32
PspOMI G'GGCC_C
Cuts at: 0 628 1245
Size: 628 617
PstI C_TGCA'G
Cuts at: 0 797 1245
Size: 797 448
PvuII CAG'CTG
Cuts at: 0 798 1245
Size: 798 447
SapI GCTCTTCn'nnn_
Cuts at: 0 219 1245
Size: 219 1026
SexAI A'CCwGG_T
Cuts at: 0 956 1245
Size: 956 289
SfcI C'TryA_G
Cuts at: 0 318 428 793 1245
Size: 318 110 365 452
Fragments arranged by size:
452 365 318 110
SfoI GGC'GCC
Cuts at: 0 889 1245
Size: 889 356
StuI AGG'CCT
Cuts at: 0 113 1055 1228 1245
Size: 113 942 173 17
Fragments arranged by size:
942 173 113 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 625 757 1032 1245
Size: 625 132 275 213
Fragments arranged by size:
625 275 213 132
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1111 1143 1245
Size: 1111 32 102
Fragments arranged by size:
1111 102 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 493 906 1245
Size: 493 413 339
Fragments arranged by size:
493 413 339
Enzymes that do cut:
AarI AccI AcuI AlfI ApaI AvaI BanI BanII
BbeI BbsI BlpI Bme1580I BmgBI BplI BpmI Bpu10I
BsaI BsaHI BsaXI BseRI BseYI BsiEI Bsp1286I BspMI
BsrBI BsrFI BssSI BstEII BstXI Bsu36I BtsI DraIII
DrdI EaeI EarI EciI Eco57MI EcoO109I HaeII Hin4I
KasI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PflMI PfoI PpiI PspOMI PstI PvuII SapI SexAI
SfcI SfoI StuI TaqII TstI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AloI AlwNI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BmrI BmtI BpuEI BsaAI BsaBI BsaWI
BsgI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrDI
BsrGI BssHII BstAPI BstBI BstYI BstZ17I BtgI BtgZI
ClaI CspCI DraI EagI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI MmeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PciI PmeI PmlI PpuMI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SbfI ScaI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StyI SwaI TatI
Tth111I XbaI XhoI XmaI XmnI ZraI