Restriction map for
pFN21AB8816
##################################
# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
AspEI AspHI AspI AvaII AviII
AvrII BbrPI BbsI BcgI BciVI
BclI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaWI BseAI BseRI BsiEI
BsiHKAI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstUI BstXI Bsu36I CelII
Cfr10I ClaI CpoI DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV FbaI FseI FspI HgaI
HincII HindII HindIII HpaI Ksp632I
KspI MamI MluI MroI MslI
MvnI NaeI NcoI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TaqI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Acc65I 224 GGTACC G^GTACC
AccI 604 GTATAC GT^[AC][GT]AC
AcyI 55 GGCGCC G[GA]^CG[CT]C
AlwNI 784 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 845 GGGCCC GGGCC^C
Asp718I 224 GGTACC G^GTACC
BalI 1097 TGGCCA TGG^CCA
BamHI 525 GGATCC G^GATCC
BbeI 55 GGCGCC GGCGC^C
BbvCI 115 GCTGAGG CCTCAGC(-5/-2)
BsaHI 55 GGCGCC G[GA]^CG[CT]C
BsaI 490 GGTCTC GGTCTC(1/5)
BsgI 658 GTGCAG GTGCAG(16/14)
BsmAI 491 GTCTC GTCTC(1/5)
BsmI 576 GAATGC GAATGC(1/-1)
BsrDI 200 GCAATG GCAATG(2/0)
Bst1107I 604 GTATAC GTA^TAC
BstEII 27 GGTGACC G^GT[ACGT]ACC
BstPI 27 GGTGACC G^GT[ACGT]ACC
BstZ17I 604 GTATAC GTA^TAC
DraI 1008 TTTAAA TTT^AAA
DraII 844 AGGGCCC [GA]G^G[ACGT]CC[CT]
EaeI 1097 TGGCCA [CT]^GGCC[GA]
EcoO109I 844 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 27 GGTGACC G^GT[ACGT]ACC
EcoT14I 232 CCTTGG C^C[AT][AT]GG
EcoT22I 455 ATGCAT ATGCA^T
FauI 760 GCGGG CCCGC(4/6)
HaeII 55 GGCGCC [GA]GCGC^[CT]
HapII 297 CCGG C^CGG
Hin1I 55 GGCGCC G[GA]^CG[CT]C
HpaII 297 CCGG C^CGG
KasI 55 GGCGCC G^GCGCC
KpnI 224 GGTACC GGTAC^C
MaeII 303 ACGT A^CGT
MfeI 497 CAATTG C^AATTG
MluNI 1097 TGGCCA TGG^CCA
MscI 1097 TGGCCA TGG^CCA
MspA1I 758 CAGCGG C[AC]G^C[GT]G
MspI 297 CCGG C^CGG
MunI 497 CAATTG C^AATTG
NarI 55 GGCGCC GG^CGCC
NdeI 1114 CATATG CA^TATG
NsiI 455 ATGCAT ATGCA^T
NspI 716 ACATGC [GA]CATG^[CT]
SfaNI 142 GATGC GCATC(5/9)
SfoI 55 GGCGCC GGC^GCC
SmaI 296 CCCGGG CCC^GGG
StuI 36 AGGCCT AGG^CCT
StyI 232 CCTTGG C^C[AT][AT]GG
TspRI 863 CACTG CA[GC]TG[ACGT][ACGT]^
XmaCI 296 CCCGGG C^CCGGG
XmaI 296 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AciI 32 CCGC CCGC(-3/-1)
760 GCGG
AfaI 225 GTAC GT^AC
853 GTAC
Asp700I 898 GAAGTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
975 GAATCAATTC
AvaI 296 CCCGGG C^[CT]CG[GA]G
521 CTCGGG
BanI 55 GGCGCC G^G[CT][GA]CC
224 GGTACC
BcnI 296 CCCGG CC^[GC]GG
297 CCGGG
BsoBI 296 CCCGGG C^[CT]CG[GA]G
521 CTCGGG
BsrI 176 ACTGG ACTGG(1/-1)
864 ACTGG
BstF5I 132 GGATG GGATG(2/0)
138 GGATG
BstYI 124 GGATCT [GA]^GATC[CT]
525 GGATCC
CfoI 56 GCGC GCG^C
591 GCGC
FokI 132 GGATG GGATG(9/13)
138 GGATG
HhaI 56 GCGC GCG^C
591 GCGC
HinP1I 56 GCGC G^CGC
591 GCGC
MflI 124 GGATCT [GA]^GATC[CT]
525 GGATCC
NciI 296 CCCGG CC^[GC]GG
297 CCGGG
RsaI 225 GTAC GT^AC
853 GTAC
SmlI 359 CTCAAG C^T[CT][GA]AG
567 CTTGAG
Tsp45I 28 GTGAC ^GT[GC]AC
1055 GTCAC
XhoII 124 GGATCT [GA]^GATC[CT]
525 GGATCC
XmnI 898 GAAGTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
975 GAATCAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AcsI AluI AlwI ApoI BanII
BbvI BfaI BmyI BsaJI BsiYI
BslI BsmFI Bsp1286I BssKI BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
Fnu4HI HaeIII HinfI HphI ItaI
MaeI MaeIII MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI TfiI
Tru9I TseI Tsp509I