Restriction map for
pFN21AB8809
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# NO CUT ENZYMES ( 150 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BcnI
BfrI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I CelII Cfr10I
ClaI CpoI DraI DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FauI FseI FspI HaeII HapII
HgaI Hin1I HincII HindII HindIII
HinfI HpaI HpaII KasI KpnI
KspI MaeIII MluI MluNI MroI
MscI MspA1I MspI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacII SalI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI SspBI
SspI StuI SwaI TfiI Tsp45I
Tth111I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccII 423 CGCG CG^CG
Alw44I 418 GTGCAC G^TGCAC
AlwNI 488 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 641 CCTTAGG CC^T[ACGT]AGG
ApaLI 418 GTGCAC G^TGCAC
Asp700I 811 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BciVI 71 GTATCC GTATCC(6/5)
BclI 671 TGATCA T^GATCA
BglI 853 GCCATCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 1057 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 793 GAGACC GGTCTC(1/5)
BseRI 1000 CTCCTC GAGGAG(10/8)
BsgI 825 CTGCAC GTGCAG(16/14)
BsmAI 793 GAGAC GTCTC(1/5)
BstUI 423 CGCG CG^CG
BstXI 987 CCAATACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 252 GGATCT [GA]^GATC[CT]
Bsu36I 641 CCTTAGG CC^T[ACGT]AGG
Cac8I 420 GCACGC GC[ACGT]^[ACGT]GC
CfoI 543 GCGC GCG^C
Eco81I 641 CCTTAGG CC^T[ACGT]AGG
FbaI 671 TGATCA T^GATCA
HhaI 543 GCGC GCG^C
HinP1I 543 GCGC G^CGC
MaeII 589 ACGT A^CGT
MamI 1057 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 413 CAATTG C^AATTG
MflI 252 GGATCT [GA]^GATC[CT]
MseI 896 TTAA T^TAA
MslI 384 CATGCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 413 CAATTG C^AATTG
MvnI 423 CGCG CG^CG
NcoI 1052 CCATGG C^CATGG
PflMI 584 CCACGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 963 CTGCAG CTGCA^G
SacI 115 GAGCTC GAGCT^C
SbfI 962 CCTGCAGG CCTGCA^GG
ScaI 569 AGTACT AGT^ACT
SmlI 435 CTTGAG C^T[CT][GA]AG
Sse8387I 962 CCTGCAGG CCTGCA^GG
TaqI 32 TCGA T^CGA
Tru9I 896 TTAA T^TAA
TthHB8I 32 TCGA T^CGA
Van91I 584 CCACGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 252 GGATCT [GA]^GATC[CT]
XmnI 811 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 12 enzymes) #
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BanII 115 GAGCTC G[GA]GC[CT]^C
997 GGGCTC
BspHI 389 TCATGA T^CATGA
1059 TCATGA
BspMI 230 ACCTGC ACCTGC(4/8)
961 ACCTGC
BsrI 303 CCAGT ACTGG(1/-1)
470 CCAGT
DraIII 421 CACGCGGTG CAC[ACGT][ACGT][ACGT]^GTG
585 CACGACGTG
HaeIII 861 GGCC GG^CC
970 GGCC
NlaIV 968 GGGGCC GG[ACGT]^[ACGT]CC
998 GGCTCC
PpuMI 445 AGGACCT [GA]G^G[AT]CC[CT]
679 AGGACCT
RcaI 389 TCATGA T^CATGA
1059 TCATGA
SfaNI 1072 GATGC GCATC(5/9)
98 GCATC
SfcI 946 CTACAG C^T[GA][CT]AG
963 CTGCAG
XcmI 303 CCAGTGGGTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
863 CCACAGAAACTCTGG
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
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AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BbvI BfaI
BmyI BsaJI BsiHKAI BsiYI BslI
Bsp1286I BssKI BstF5I BstNI Cfr13I
DdeI DpnII DraII DsaI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HphI ItaI Ksp632I MaeI MboI
MboII MnlI MvaI MwoI NdeII
NlaIII RsaI SapI Sau3AI Sau96I
ScrFI StyI TseI Tsp509I TspRI