(Circular) (Six-base) MAPSORT of: pFN21AB8809.seq Check: 2120 from: 1 to: 5785 pFN21AB8809 5785 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3595 278 Size: 53 83 186 936 2059 2468 Fragments arranged by size: 2468 2059 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3128 1937 Size: 1191 4594 Acc65I G'GTAC_C Cuts at: 3106 3106 Size: 5785 AclI AA'CG_TT Cuts at: 1563 3912 4285 1563 Size: 2349 373 3063 Fragments arranged by size: 3063 2349 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2807 3848 5232 5500 1632 Size: 1175 1041 1384 268 1917 Fragments arranged by size: 1917 1384 1175 1041 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5588 AflIII A'CryG_T Cuts at: 1203 4684 5661 1203 Size: 3481 977 1327 Fragments arranged by size: 3481 1327 977 AgeI A'CCGG_T Cuts at: 4616 4616 Size: 5785 AhdI GACnn_n'nnGTC Cuts at: 4514 4514 Size: 5785 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5785 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5785 AlwNI CAG_nnn'CTG Cuts at: 2498 3528 5100 2498 Size: 1030 1572 3183 Fragments arranged by size: 3183 1572 1030 ApaLI G'TGCA_C Cuts at: 2423 3842 4998 2423 Size: 1419 1156 3210 Fragments arranged by size: 3210 1419 1156 ApoI r'AATT_y Cuts at: 1493 2173 2933 3012 3094 3320 3472 5581 1493 Size: 680 760 79 82 226 152 2109 1697 Fragments arranged by size: 2109 1697 760 680 226 152 82 79 AseI AT'TA_AT Cuts at: 160 1032 4339 160 Size: 872 3307 1606 Fragments arranged by size: 3307 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5785 AvaI C'yCGr_G Cuts at: 1870 1943 3110 1870 Size: 73 1167 4545 Fragments arranged by size: 4545 1167 73 BamHI G'GATC_C Cuts at: 3115 3482 3115 Size: 367 5418 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3106 4561 618 Size: 325 217 627 1319 1455 1842 Fragments arranged by size: 1842 1455 1319 627 325 217 BanII G_rGCy'C Cuts at: 1391 2124 3006 3104 1391 Size: 733 882 98 4072 Fragments arranged by size: 4072 882 733 98 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4814 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3976 4010 1903 Size: 34 2039 34 3678 Fragments arranged by size: 3678 2039 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2087 3677 4887 2087 Size: 1590 1210 2985 Fragments arranged by size: 2985 1590 1210 BclI T'GATC_A Cuts at: 1548 2676 1548 Size: 1128 4657 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2864 4396 136 Size: 107 122 71 631 605 1192 1532 1525 Fragments arranged by size: 1532 1525 1192 631 605 122 107 71 BglII A'GATC_T Cuts at: 5780 5780 Size: 5785 BlpI GC'TnA_GC Cuts at: 3202 3202 Size: 5785 Bme1580I G_kGCm'C Cuts at: 1790 2427 3846 5002 1790 Size: 637 1419 1156 2573 Fragments arranged by size: 2573 1419 1156 637 BmgBI CAC'GTC Cuts at: 1318 2595 1318 Size: 1277 4508 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3516 4474 442 Size: 416 53 83 399 24 2099 958 1753 Fragments arranged by size: 2099 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5785 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4445 1239 Size: 3206 2579 Bpu10I CC'TnA_GC Cuts at: 2213 2213 Size: 5785 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2461 3910 4775 5073 2461 Size: 1449 865 298 3173 Fragments arranged by size: 3173 1449 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2792 4448 882 Size: 617 1293 1656 2219 Fragments arranged by size: 2219 1656 1293 617 BsaAI yAC'GTr Cuts at: 493 1204 3576 493 Size: 711 2372 2702 Fragments arranged by size: 2702 2372 711 BsaBI GATnn'nnATC Cuts at: 3066 5779 3066 Size: 2713 3072 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3592 3974 4609 275 Size: 53 83 186 936 2059 382 635 1451 Fragments arranged by size: 2059 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4218 4616 4890 5037 5448 4218 Size: 398 274 147 411 4555 Fragments arranged by size: 4555 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5560 Fragments arranged by size: 5560 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2996 1186 Size: 1810 3975 BseYI C'CCAG_C Cuts at: 3032 4988 5484 3032 Size: 1956 496 3333 Fragments arranged by size: 3333 1956 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2815 1163 Size: 573 1079 4133 Fragments arranged by size: 4133 1079 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3235 3996 5024 1540 Size: 275 186 1234 761 1028 2301 Fragments arranged by size: 2301 1234 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2124 2427 2790 2988 3846 3931 5002 2124 Size: 303 363 198 858 85 1071 2907 Fragments arranged by size: 2907 1071 858 363 303 198 85 BsmI GAATG_Cn' Cuts at: 3296 3389 3296 Size: 93 5692 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2124 2427 2790 2988 3006 3104 3846 Size: 399 334 303 363 198 18 98 742 Cuts at: 3846 3931 5002 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 363 334 303 198 98 85 18 BspEI T'CCGG_A Cuts at: 5448 5448 Size: 5785 BspHI T'CATG_A Cuts at: 2394 3064 3674 5404 2394 Size: 670 610 1730 2775 Fragments arranged by size: 2775 1730 670 610 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2244 2975 3140 844 Size: 1400 731 165 3489 Fragments arranged by size: 3489 1400 731 165 BsrBI CCG'CTC Cuts at: 1394 1823 3672 1394 Size: 429 1849 3507 Fragments arranged by size: 3507 1849 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4280 4454 65 Size: 1183 3032 174 1396 Fragments arranged by size: 3032 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4429 4616 1700 Size: 110 2619 187 2869 Fragments arranged by size: 2869 2619 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5785 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5785 BssSI C'ACGA_G Cuts at: 3845 4857 3845 Size: 1012 4773 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5785 BstBI TT'CG_AA Cuts at: 4589 4589 Size: 5785 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5785 BstXI CCAn_nnnn'nTGG Cuts at: 2999 2999 Size: 5785 BstYI r'GATC_y Cuts at: 1972 2257 3115 3482 3867 3884 5336 5422 5434 Size: 285 858 367 385 17 1452 86 12 Cuts at: 5434 5780 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 858 385 367 346 285 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2647 2647 Size: 5785 BtgI C'CryG_G Cuts at: 513 1065 2013 2742 3057 513 Size: 552 948 729 315 3241 Fragments arranged by size: 3241 948 729 552 315 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5046 Fragments arranged by size: 5046 683 56 BtsI GCAGTG_nn' Cuts at: 2890 3301 4095 4115 2890 Size: 411 794 20 4560 Fragments arranged by size: 4560 794 411 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5785 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5750 DraI TTT'AAA Cuts at: 3089 3089 Size: 5785 DraIII CAC_nnn'GTG Cuts at: 2431 2595 2431 Size: 164 5621 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4792 809 Size: 507 3476 1802 Fragments arranged by size: 3476 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3232 4121 5750 Size: 54 1425 50 275 18 1402 889 1629 Cuts at: 5750 8 Size: 43 Fragments arranged by size: 1629 1425 1402 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3232 1537 Size: 275 1420 4090 Fragments arranged by size: 4090 1420 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2129 2270 2759 2795 2963 3715 1353 Size: 776 141 489 36 168 752 3423 Fragments arranged by size: 3423 776 752 489 168 141 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4372 4746 4892 387 Size: 1064 138 2783 374 146 1280 Fragments arranged by size: 2783 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2122 2122 Size: 5785 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2807 3848 4445 5232 5500 1239 Size: 393 1175 1041 597 787 268 1524 Fragments arranged by size: 1524 1175 1041 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2451 2685 2974 2451 Size: 234 289 5262 Fragments arranged by size: 5262 289 234 EcoRI G'AATT_C Cuts at: 3094 3472 3094 Size: 378 5407 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2752 2752 Size: 5785 FspI TGC'GCA Cuts at: 4291 4291 Size: 5785 HaeII r_GCGC'y Cuts at: 1678 4932 1678 Size: 3254 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2625 2657 4426 4458 4500 Size: 32 135 32 1053 32 1769 32 42 Cuts at: 4500 4532 1373 Size: 32 2626 Fragments arranged by size: 2626 1769 1053 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3129 3375 669 Size: 988 281 1191 246 3079 Fragments arranged by size: 3079 1191 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5785 HpaI GTT'AAC Cuts at: 3375 3375 Size: 5785 KpnI G_GTAC'C Cuts at: 3110 3110 Size: 5785 MfeI C'AATT_G Cuts at: 2418 3384 2418 Size: 966 4819 MluI A'CGCG_T Cuts at: 5661 5661 Size: 5785 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4899 5083 1305 Size: 3594 184 2007 Fragments arranged by size: 3594 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3963 Fragments arranged by size: 3963 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2393 3743 4102 4261 518 Size: 648 1227 1350 359 159 2042 Fragments arranged by size: 2042 1350 1227 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3201 3878 5026 5271 1606 Size: 1595 677 1148 245 2120 Fragments arranged by size: 2120 1595 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 3057 513 Size: 552 1992 3241 Fragments arranged by size: 3241 1992 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5785 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5785 NotI GC'GGCC_GC Cuts at: 3232 3232 Size: 5785 NspI r_CATG'y Cuts at: 1132 1229 3143 4688 1132 Size: 97 1914 1545 2229 Fragments arranged by size: 2229 1914 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5785 PciI A'CATG_T Cuts at: 4684 4684 Size: 5785 PflMI CCAn_nnn'nTGG Cuts at: 1108 2595 1108 Size: 1487 4298 PfoI T'CCnGG_A Cuts at: 2345 2345 Size: 5785 PmeI GTTT'AAAC Cuts at: 3089 3089 Size: 5785 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3854 3886 5367 5399 3854 Size: 32 1481 32 4240 Fragments arranged by size: 4240 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2451 2685 2451 Size: 234 5551 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5785 PsiI TTA'TAA Cuts at: 3355 3355 Size: 5785 PstI C_TGCA'G Cuts at: 830 2972 3137 830 Size: 2142 165 3478 Fragments arranged by size: 3478 2142 165 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5785 SacI G_AGCT'C Cuts at: 2124 2124 Size: 5785 SalI G'TCGA_C Cuts at: 1936 3127 1936 Size: 1191 4594 SapI GCTCTTCn'nnn_ Cuts at: 2129 2270 2795 2963 2129 Size: 141 525 168 4951 Fragments arranged by size: 4951 525 168 141 SbfI CC_TGCA'GG Cuts at: 2972 3137 2972 Size: 165 5620 ScaI AGT'ACT Cuts at: 2576 4033 5745 2576 Size: 1457 1712 2616 Fragments arranged by size: 2616 1712 1457 SfcI C'TryA_G Cuts at: 826 1045 2951 2968 3133 4268 4949 5140 826 Size: 219 1906 17 165 1135 681 191 1471 Fragments arranged by size: 1906 1471 1135 681 219 191 165 17 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5785 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5785 SmaI CCC'GGG Cuts at: 1872 3112 1872 Size: 1240 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2440 3889 4790 5052 820 Size: 197 926 497 1449 901 262 1553 Fragments arranged by size: 1553 1449 926 901 497 262 197 SnaBI TAC'GTA Cuts at: 493 3576 493 Size: 3083 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5785 SphI G_CATG'C Cuts at: 1132 3143 1132 Size: 2011 3774 SspI AAT'ATT Cuts at: 5 52 3709 5 Size: 47 3657 2081 Fragments arranged by size: 3657 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5785 StyI C'CwwG_G Cuts at: 513 1065 2145 2758 3057 3224 513 Size: 552 1080 613 299 167 3074 Fragments arranged by size: 3074 1080 613 552 299 167 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3812 3829 3982 4167 4612 325 Size: 1255 2232 17 153 185 445 1498 Fragments arranged by size: 2232 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2352 2574 2943 Size: 274 80 33 51 1443 375 222 369 Cuts at: 2943 4031 5743 96 Size: 1088 1712 138 Fragments arranged by size: 1712 1443 1088 375 369 274 222 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2163 2195 5745 5777 2163 Size: 32 3550 32 2171 Fragments arranged by size: 3550 2171 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5785 XbaI T'CTAG_A Cuts at: 3121 3121 Size: 5785 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2315 2875 2315 Size: 560 5225 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5785 XmaI C'CCGG_G Cuts at: 1870 3110 1870 Size: 1240 4545 XmnI GAAnn'nnTTC Cuts at: 2820 3914 2820 Size: 1094 4691 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3593 276 Size: 53 83 186 936 2059 2468 Fragments arranged by size: 2468 2059 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI