(Circular) (Six-base) MAPSORT of: pFN21AB8798.seq Check: 880 from: 1 to: 5713 pFN21AB8798 5713 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3523 278 Size: 53 83 186 936 1987 2468 Fragments arranged by size: 2468 1987 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2520 3056 1937 Size: 583 536 4594 Fragments arranged by size: 4594 583 536 Acc65I G'GTAC_C Cuts at: 3034 3034 Size: 5713 AclI AA'CG_TT Cuts at: 1563 3840 4213 1563 Size: 2277 373 3063 Fragments arranged by size: 3063 2277 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3776 5160 5428 1632 Size: 2144 1384 268 1917 Fragments arranged by size: 2144 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5516 AflIII A'CryG_T Cuts at: 1203 2021 4612 5589 1203 Size: 818 2591 977 1327 Fragments arranged by size: 2591 1327 977 818 AgeI A'CCGG_T Cuts at: 4544 4544 Size: 5713 AhdI GACnn_n'nnGTC Cuts at: 4442 4442 Size: 5713 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5713 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2222 1184 Size: 1038 4675 AlwNI CAG_nnn'CTG Cuts at: 3456 5028 3456 Size: 1572 4141 ApaLI G'TGCA_C Cuts at: 3770 4926 3770 Size: 1156 4557 ApoI r'AATT_y Cuts at: 1493 2438 3022 3248 3400 5509 1493 Size: 945 584 226 152 2109 1697 Fragments arranged by size: 2109 1697 945 584 226 152 AseI AT'TA_AT Cuts at: 160 1032 4267 160 Size: 872 3235 1606 Fragments arranged by size: 3235 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5713 AvaI C'yCGr_G Cuts at: 1870 1943 3038 1870 Size: 73 1095 4545 Fragments arranged by size: 4545 1095 73 BamHI G'GATC_C Cuts at: 3043 3410 3043 Size: 367 5346 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3034 4489 618 Size: 325 217 627 1247 1455 1842 Fragments arranged by size: 1842 1455 1247 627 325 217 BanII G_rGCy'C Cuts at: 1391 3032 1391 Size: 1641 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2963 928 Size: 971 1064 3678 Fragments arranged by size: 3678 1064 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3904 3938 1903 Size: 34 1967 34 3678 Fragments arranged by size: 3678 1967 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3605 4815 3605 Size: 1210 4503 BclI T'GATC_A Cuts at: 1548 1548 Size: 5713 BfrBI ATG'CAT Cuts at: 2302 2472 2302 Size: 170 5543 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4324 136 Size: 107 122 71 631 605 2652 1525 Fragments arranged by size: 2652 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5708 5708 Size: 5713 BlpI GC'TnA_GC Cuts at: 3130 3130 Size: 5713 Bme1580I G_kGCm'C Cuts at: 1790 3774 4930 1790 Size: 1984 1156 2573 Fragments arranged by size: 2573 1984 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5713 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2504 3444 4402 Size: 416 53 83 399 24 1087 940 958 Cuts at: 4402 442 Size: 1753 Fragments arranged by size: 1753 1087 958 940 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5713 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4373 1239 Size: 3134 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3838 4703 5001 3838 Size: 865 298 4550 Fragments arranged by size: 4550 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2618 4376 882 Size: 617 1119 1758 2219 Fragments arranged by size: 2219 1758 1119 617 BsaAI yAC'GTr Cuts at: 493 1204 3504 493 Size: 711 2300 2702 Fragments arranged by size: 2702 2300 711 BsaBI GATnn'nnATC Cuts at: 5707 5707 Size: 5713 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3520 3902 4537 275 Size: 53 83 186 936 1987 382 635 1451 Fragments arranged by size: 1987 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4146 4544 4818 4965 5376 4146 Size: 398 274 147 411 4483 Fragments arranged by size: 4483 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5488 Fragments arranged by size: 5488 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5713 BseYI C'CCAG_C Cuts at: 4916 5412 4916 Size: 496 5217 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2218 1163 Size: 573 482 4658 Fragments arranged by size: 4658 573 482 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3163 3924 4952 1540 Size: 275 186 1162 761 1028 2301 Fragments arranged by size: 2301 1162 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3774 3859 4930 3774 Size: 85 1071 4557 Fragments arranged by size: 4557 1071 85 BsmI GAATG_Cn' Cuts at: 3224 3317 3224 Size: 93 5620 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3032 3774 3859 4930 1391 Size: 399 1242 742 85 1071 2174 Fragments arranged by size: 2174 1242 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5376 5376 Size: 5713 BspHI T'CATG_A Cuts at: 2214 3602 5332 2214 Size: 1388 1730 2595 Fragments arranged by size: 2595 1730 1388 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2600 3068 844 Size: 1756 468 3489 Fragments arranged by size: 3489 1756 468 BsrBI CCG'CTC Cuts at: 1394 1823 3600 1394 Size: 429 1777 3507 Fragments arranged by size: 3507 1777 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4208 4382 65 Size: 1183 2960 174 1396 Fragments arranged by size: 2960 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4357 4544 1700 Size: 110 2547 187 2869 Fragments arranged by size: 2869 2547 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5713 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5713 BssSI C'ACGA_G Cuts at: 3773 4785 3773 Size: 1012 4701 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5713 BstBI TT'CG_AA Cuts at: 4517 4517 Size: 5713 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5713 BstYI r'GATC_y Cuts at: 1972 3043 3410 3795 3812 5264 5350 5362 5708 Size: 1071 367 385 17 1452 86 12 346 Cuts at: 5708 1972 Size: 1977 Fragments arranged by size: 1977 1452 1071 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2521 2521 Size: 5713 BtgI C'CryG_G Cuts at: 513 1065 2049 513 Size: 552 984 4177 Fragments arranged by size: 4177 984 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4974 Fragments arranged by size: 4974 683 56 BtsI GCAGTG_nn' Cuts at: 2206 3229 4023 4043 2206 Size: 1023 794 20 3876 Fragments arranged by size: 3876 1023 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5713 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2614 2649 529 Size: 35 2050 35 3593 Fragments arranged by size: 3593 2050 35 35 DraI TTT'AAA Cuts at: 2809 3017 2809 Size: 208 5505 DraIII CAC_nnn'GTG Cuts at: 2176 2176 Size: 5713 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4720 809 Size: 507 3404 1802 Fragments arranged by size: 3404 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2422 2901 3160 Size: 54 1425 50 275 18 592 479 259 Cuts at: 3160 4049 5678 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 592 479 275 259 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3160 1537 Size: 275 1348 4090 Fragments arranged by size: 4090 1348 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2050 2547 3643 1353 Size: 697 497 1096 3423 Fragments arranged by size: 3423 1096 697 497 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4300 4674 4820 387 Size: 1064 138 2711 374 146 1280 Fragments arranged by size: 2711 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3776 4373 5160 5428 1239 Size: 393 2144 597 787 268 1524 Fragments arranged by size: 2144 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 3022 3400 3022 Size: 378 5335 FspI TGC'GCA Cuts at: 4219 4219 Size: 5713 HaeII r_GCGC'y Cuts at: 1678 4860 1678 Size: 3182 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2580 2596 2612 2628 4354 Size: 32 135 32 1008 16 16 16 1726 Cuts at: 4354 4386 4428 4460 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1726 1008 135 42 32 32 32 32 16 16 16 HincII GTy'rAC Cuts at: 669 1657 1938 3057 3303 669 Size: 988 281 1119 246 3079 Fragments arranged by size: 3079 1119 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5713 HpaI GTT'AAC Cuts at: 3303 3303 Size: 5713 KpnI G_GTAC'C Cuts at: 3038 3038 Size: 5713 MfeI C'AATT_G Cuts at: 2852 3312 2852 Size: 460 5253 MluI A'CGCG_T Cuts at: 5589 5589 Size: 5713 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2318 4827 5011 1305 Size: 1013 2509 184 2007 Fragments arranged by size: 2509 2007 1013 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2424 10 Size: 54 1425 343 592 3299 Fragments arranged by size: 3299 1425 592 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3671 4030 4189 518 Size: 648 2505 359 159 2042 Fragments arranged by size: 2505 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3129 3806 4954 5199 1606 Size: 1523 677 1148 245 2120 Fragments arranged by size: 2120 1523 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2049 513 Size: 552 984 4177 Fragments arranged by size: 4177 984 552 NdeI CA'TA_TG Cuts at: 387 2480 2997 3007 387 Size: 2093 517 10 3093 Fragments arranged by size: 3093 2093 517 10 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5713 NotI GC'GGCC_GC Cuts at: 3160 3160 Size: 5713 NsiI A_TGCA'T Cuts at: 2304 2474 2304 Size: 170 5543 NspI r_CATG'y Cuts at: 1132 1229 2025 2306 3071 4616 1132 Size: 97 796 281 765 1545 2229 Fragments arranged by size: 2229 1545 796 765 281 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5713 PciI A'CATG_T Cuts at: 2021 4612 2021 Size: 2591 3122 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5713 PmeI GTTT'AAAC Cuts at: 3017 3017 Size: 5713 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3782 3814 5295 5327 3782 Size: 32 1481 32 4168 Fragments arranged by size: 4168 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5713 PsiI TTA'TAA Cuts at: 3283 3283 Size: 5713 PstI C_TGCA'G Cuts at: 830 3065 830 Size: 2235 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5713 SalI G'TCGA_C Cuts at: 1936 3055 1936 Size: 1119 4594 SbfI CC_TGCA'GG Cuts at: 3065 3065 Size: 5713 ScaI AGT'ACT Cuts at: 2655 3961 5673 2655 Size: 1306 1712 2695 Fragments arranged by size: 2695 1712 1306 SfcI C'TryA_G Cuts at: 826 1045 2541 3061 4196 4877 5068 826 Size: 219 1496 520 1135 681 191 1471 Fragments arranged by size: 1496 1471 1135 681 520 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5713 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5713 SmaI CCC'GGG Cuts at: 1872 3040 1872 Size: 1168 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3817 4718 4980 820 Size: 197 926 1874 901 262 1553 Fragments arranged by size: 1874 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3504 493 Size: 3011 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5713 SphI G_CATG'C Cuts at: 1132 2306 3071 1132 Size: 1174 765 3774 Fragments arranged by size: 3774 1174 765 SspI AAT'ATT Cuts at: 5 52 2415 3637 5 Size: 47 2363 1222 2081 Fragments arranged by size: 2363 2081 1222 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5713 StyI C'CwwG_G Cuts at: 513 1065 2049 3152 513 Size: 552 984 1103 3074 Fragments arranged by size: 3074 1103 984 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3740 3757 3910 4095 4540 325 Size: 1255 2160 17 153 185 445 1498 Fragments arranged by size: 2160 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2491 2653 3959 Size: 274 80 33 51 1443 514 162 1306 Cuts at: 3959 5671 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1306 514 274 162 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5673 5705 5673 Size: 32 5681 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5713 XbaI T'CTAG_A Cuts at: 3049 3049 Size: 5713 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5713 XmaI C'CCGG_G Cuts at: 1870 3038 1870 Size: 1168 4545 XmnI GAAnn'nnTTC Cuts at: 2237 3842 2237 Size: 1605 4108 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3521 276 Size: 53 83 186 936 1987 2468 Fragments arranged by size: 2468 1987 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI