Restriction map for
pFN21AB8786
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII AccI AccII AclI AcsI
AcyI AflII AgeI AhdI AocI
Aor51HI ApoI AscI AseI AsnI
AspEI AspI AviII AvrII BamHI
BbeI BbvCI BcgI BciVI BcnI
BfaI BfrI BglI BlnI BlpI
Bpu1102I BsaBI BsaHI BsaI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspHI
BsrBI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HaeII Hin1I HincII
HindII HindIII HpaI KasI KspI
MaeI MamI MfeI MluI MseI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tru9I
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 410 GGTACC G^GTACC
AccIII 833 TCCGGA T^CCGGA
Alw44I 580 GTGCAC G^TGCAC
AlwI 602 GATCC GGATC(4/5)
AlwNI 882 CAGGTACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 695 GGGCCC GGGCC^C
ApaLI 580 GTGCAC G^TGCAC
Asp700I 47 GAAATAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 410 GGTACC G^GTACC
AvaI 673 CTCGGG C^[CT]CG[GA]G
AvaII 378 GGACC G^G[AT]CC
BalI 197 TGGCCA TGG^CCA
BbrPI 281 CACGTG CAC^GTG
BpmI 163 CTCCAG CTGGAG(16/14)
BsaAI 281 CACGTG [CT]AC^GT[GA]
BsaJI 491 CCATGG C^C[ACGT][ACGT]GG
BsaWI 833 TCCGGA [AT]^CCGG[AT]
BseAI 833 TCCGGA T^CCGGA
BseRI 82 GAGGAG GAGGAG(10/8)
BsgI 471 CTGCAC GTGCAG(16/14)
BsmAI 765 GTCTC GTCTC(1/5)
BsmI 856 GCATTC GAATGC(1/-1)
BsoBI 673 CTCGGG C^[CT]CG[GA]G
BspEI 833 TCCGGA T^CCGGA
BspMI 881 GCAGGT ACCTGC(4/8)
BsrDI 800 GCAATG GCAATG(2/0)
BssSI 65 CTCGTG CACGAG(-5/-1)
CpoI 377 CGGACCG CG^G[AT]CCG
DdeI 847 CTCAG C^T[ACGT]AG
DraII 695 GGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 491 CCATGG C^C[GA][CT]GG
EaeI 197 TGGCCA [CT]^GGCC[GA]
EarI 769 CTCTTC CTCTTC(1/4)
EcoO109I 695 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 491 CCATGG C^C[AT][AT]GG
FauI 546 CCCGC CCCGC(4/6)
HgaI 853 GACGC GACGC(5/10)
KpnI 410 GGTACC GGTAC^C
Ksp632I 769 CTCTTC CTCTTC(1/4)
MluNI 197 TGGCCA TGG^CCA
MroI 833 TCCGGA T^CCGGA
MscI 197 TGGCCA TGG^CCA
MspA1I 547 CCGCTG C[AC]G^C[GT]G
MwoI 540 GCTGTTCCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 491 CCATGG C^CATGG
PleI 319 GACTC GAGTC(4/5)
PmaCI 281 CACGTG CAC^GTG
PmlI 281 CACGTG CAC^GTG
PstI 876 CTGCAG CTGCA^G
RsrII 377 CGGACCG CG^G[AT]CCG
SacI 844 GAGCTC GAGCT^C
SmlI 274 CTCAAG C^T[CT][GA]AG
StyI 491 CCATGG C^C[AT][AT]GG
Tsp45I 424 GTGAC ^GT[GC]AC
XmnI 47 GAAATAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflIII 584 ACATGT A^C[GA][CT]GT
761 ACATGT
AluI 193 AGCT AG^CT
845 AGCT
AspHI 580 GTGCAC G[AT]GC[AT]^C
844 GAGCTC
BanI 168 GGCACC G^G[CT][GA]CC
410 GGTACC
BbsI 559 GTCTTC GAAGAC(2/6)
679 GTCTTC
BglII 230 AGATCT A^GATCT
617 AGATCT
BpuAI 559 GTCTTC GAAGAC(2/6)
679 GTCTTC
BsiHKAI 580 GTGCAC G[AT]GC[AT]^C
844 GAGCTC
BsmFI 478 GGGAC GGGAC(10/14)
851 GGGAC
BspLU11I 584 ACATGT A^CATGT
761 ACATGT
BsrI 481 ACTGG ACTGG(1/-1)
887 ACTGG
BstF5I 564 CATCC GGATG(2/0)
666 CATCC
BstYI 230 AGATCT [GA]^GATC[CT]
617 AGATCT
CfoI 432 GCGC GCG^C
752 GCGC
FokI 564 CATCC GGATG(9/13)
666 CATCC
HapII 79 CCGG C^CGG
834 CCGG
HhaI 432 GCGC GCG^C
752 GCGC
HinP1I 432 GCGC G^CGC
752 GCGC
HpaII 79 CCGG C^CGG
834 CCGG
MaeIII 424 GTGAC ^GT[ACGT]AC
740 GTAAC
MflI 230 AGATCT [GA]^GATC[CT]
617 AGATCT
MspI 79 CCGG C^CGG
834 CCGG
SfcI 189 CTGTAG C^T[GA][CT]AG
876 CTGCAG
TaqI 317 TCGA T^CGA
600 TCGA
TfiI 123 GAATC G^A[AT]TC
159 GAATC
Tsp509I 52 AATT ^AATT
99 AATT
TthHB8I 317 TCGA T^CGA
600 TCGA
XhoII 230 AGATCT [GA]^GATC[CT]
617 AGATCT
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AfaI BanII BbvI BclI
BmyI BsiYI BslI Bsp1286I BssKI
BstNI Cac8I Cfr13I DpnII EcoRII
FbaI Fnu4HI HaeIII HinfI HphI
ItaI MaeII MboI MboII MnlI
MslI MvaI NdeII NlaIII NlaIV
NspI RsaI Sau3AI Sau96I ScrFI
SfaNI TseI TspRI