(Circular) (Six-base) MAPSORT of: pFN21AB8786.seq Check: 1327 from: 1 to: 5596 pFN21AB8786 5596 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3406 278 Size: 53 83 186 936 1870 2468 Fragments arranged by size: 2468 1870 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2939 1937 Size: 1002 4594 Acc65I G'GTAC_C Cuts at: 2415 2917 2415 Size: 502 5094 AclI AA'CG_TT Cuts at: 1563 3723 4096 1563 Size: 2160 373 3063 Fragments arranged by size: 3063 2160 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2344 3659 5043 5311 1632 Size: 712 1315 1384 268 1917 Fragments arranged by size: 1917 1384 1315 712 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5399 AflIII A'CryG_T Cuts at: 1203 2589 2766 4495 5472 1203 Size: 1386 177 1729 977 1327 Fragments arranged by size: 1729 1386 1327 977 177 AgeI A'CCGG_T Cuts at: 4427 4427 Size: 5596 AhdI GACnn_n'nnGTC Cuts at: 4325 4325 Size: 5596 AleI CACnn'nnGTG Cuts at: 1166 2447 2498 1166 Size: 1281 51 4264 Fragments arranged by size: 4264 1281 51 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5596 AlwNI CAG_nnn'CTG Cuts at: 2892 3339 4911 2892 Size: 447 1572 3577 Fragments arranged by size: 3577 1572 447 ApaI G_GGCC'C Cuts at: 2704 2704 Size: 5596 ApaLI G'TGCA_C Cuts at: 2585 3653 4809 2585 Size: 1068 1156 3372 Fragments arranged by size: 3372 1156 1068 ApoI r'AATT_y Cuts at: 1493 2905 3131 3283 5392 1493 Size: 1412 226 152 2109 1697 Fragments arranged by size: 2109 1697 1412 226 152 AseI AT'TA_AT Cuts at: 160 1032 4150 160 Size: 872 3118 1606 Fragments arranged by size: 3118 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5596 AvaI C'yCGr_G Cuts at: 1870 1943 2678 2921 1870 Size: 73 735 243 4545 Fragments arranged by size: 4545 735 243 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2399 2432 2399 Size: 33 5563 BamHI G'GATC_C Cuts at: 2926 3293 2926 Size: 367 5229 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2173 2415 2917 4372 618 Size: 325 217 627 386 242 502 1455 1842 Fragments arranged by size: 1842 1455 627 502 386 325 242 217 BanII G_rGCy'C Cuts at: 1391 2704 2846 2853 2915 1391 Size: 1313 142 7 62 4072 Fragments arranged by size: 4072 1313 142 62 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2557 2677 928 Size: 971 658 120 3847 Fragments arranged by size: 3847 971 658 120 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3787 3821 1903 Size: 34 1850 34 3678 Fragments arranged by size: 3678 1850 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3488 4698 3488 Size: 1210 4386 BclI T'GATC_A Cuts at: 1548 2253 2529 2796 1548 Size: 705 276 267 4348 Fragments arranged by size: 4348 705 276 267 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4207 136 Size: 107 122 71 631 605 2535 1525 Fragments arranged by size: 2535 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2235 2622 5591 2235 Size: 387 2969 2240 Fragments arranged by size: 2969 2240 387 BlpI GC'TnA_GC Cuts at: 3013 3013 Size: 5596 Bme1580I G_kGCm'C Cuts at: 1790 2523 2589 2704 3657 4813 1790 Size: 733 66 115 953 1156 2573 Fragments arranged by size: 2573 1156 953 733 115 66 BmgBI CAC'GTC Cuts at: 1318 2541 1318 Size: 1223 4373 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2902 3327 4285 Size: 416 53 83 399 24 1485 425 958 Cuts at: 4285 442 Size: 1753 Fragments arranged by size: 1753 1485 958 425 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5596 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2153 4256 1239 Size: 914 2103 2579 Fragments arranged by size: 2579 2103 914 Bpu10I CC'TnA_GC Cuts at: 2852 2852 Size: 5596 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2264 3721 4586 4884 2264 Size: 1457 865 298 2976 Fragments arranged by size: 2976 1457 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4259 882 Size: 617 2760 2219 Fragments arranged by size: 2760 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2288 3387 493 Size: 711 1084 1099 2702 Fragments arranged by size: 2702 1099 1084 711 BsaBI GATnn'nnATC Cuts at: 5590 5590 Size: 5596 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3403 3785 4420 275 Size: 53 83 186 936 1870 382 635 1451 Fragments arranged by size: 1870 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2838 4029 4427 4701 4848 5259 2838 Size: 1191 398 274 147 411 3175 Fragments arranged by size: 3175 1191 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5371 Fragments arranged by size: 5371 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2102 1186 Size: 916 4680 BseYI C'CCAG_C Cuts at: 2218 2697 4799 5295 2218 Size: 479 2102 496 2519 Fragments arranged by size: 2519 2102 496 479 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2461 1163 Size: 573 725 4298 Fragments arranged by size: 4298 725 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3046 3807 4835 1540 Size: 275 186 1045 761 1028 2301 Fragments arranged by size: 2301 1045 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2589 2853 3657 3742 4813 2589 Size: 264 804 85 1071 3372 Fragments arranged by size: 3372 1071 804 264 85 BsmI GAATG_Cn' Cuts at: 2861 3107 3200 2861 Size: 246 93 5257 Fragments arranged by size: 5257 246 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2523 2589 2704 2846 2853 2915 3657 Size: 399 733 66 115 142 7 62 742 Cuts at: 3657 3742 4813 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 733 399 142 115 85 66 62 7 BspEI T'CCGG_A Cuts at: 2838 5259 2838 Size: 2421 3175 BspHI T'CATG_A Cuts at: 3485 5215 3485 Size: 1730 3866 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2877 2951 844 Size: 2033 74 3489 Fragments arranged by size: 3489 2033 74 BsrBI CCG'CTC Cuts at: 1394 1823 3483 1394 Size: 429 1660 3507 Fragments arranged by size: 3507 1660 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2812 4091 4265 65 Size: 1183 1564 1279 174 1396 Fragments arranged by size: 1564 1396 1279 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4240 4427 1700 Size: 110 2430 187 2869 Fragments arranged by size: 2869 2430 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5596 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5596 BssSI C'ACGA_G Cuts at: 2070 3656 4668 2070 Size: 1586 1012 2998 Fragments arranged by size: 2998 1586 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5596 BstBI TT'CG_AA Cuts at: 4400 4400 Size: 5596 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5596 BstYI r'GATC_y Cuts at: 1972 2235 2622 2926 3293 3678 3695 5147 5233 Size: 263 387 304 367 385 17 1452 86 Cuts at: 5233 5245 5591 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 387 385 367 346 304 263 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2496 513 Size: 552 1431 3613 Fragments arranged by size: 3613 1431 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4857 Fragments arranged by size: 4857 683 56 BtsI GCAGTG_nn' Cuts at: 2408 3112 3906 3926 2408 Size: 704 794 20 4078 Fragments arranged by size: 4078 794 704 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5596 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2269 2304 529 Size: 35 1705 35 3821 Fragments arranged by size: 3821 1705 35 35 DraI TTT'AAA Cuts at: 2900 2900 Size: 5596 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4603 809 Size: 507 3287 1802 Fragments arranged by size: 3287 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2202 3043 3932 Size: 54 1425 50 275 18 372 841 889 Cuts at: 3932 5561 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 841 372 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3043 1537 Size: 275 1231 4090 Fragments arranged by size: 4090 1231 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2780 3526 1353 Size: 1427 746 3423 Fragments arranged by size: 3423 1427 746 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4183 4557 4703 387 Size: 1064 138 2594 374 146 1280 Fragments arranged by size: 2594 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2851 2851 Size: 5596 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2153 2344 3659 4256 5043 5311 1239 Size: 393 521 191 1315 597 787 268 1524 Fragments arranged by size: 1524 1315 787 597 521 393 268 191 EcoO109I rG'GnC_Cy Cuts at: 2701 2701 Size: 5596 EcoRI G'AATT_C Cuts at: 2905 3283 2905 Size: 378 5218 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2893 2893 Size: 5596 FspI TGC'GCA Cuts at: 4102 4102 Size: 5596 HaeII r_GCGC'y Cuts at: 1678 4743 1678 Size: 3065 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4237 4269 4311 4343 1373 Size: 32 135 32 2665 32 42 32 2626 Fragments arranged by size: 2665 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2940 3186 669 Size: 988 281 1002 246 3079 Fragments arranged by size: 3079 1002 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5596 HpaI GTT'AAC Cuts at: 3186 3186 Size: 5596 KpnI G_GTAC'C Cuts at: 2419 2921 2419 Size: 502 5094 MfeI C'AATT_G Cuts at: 3195 3195 Size: 5596 MluI A'CGCG_T Cuts at: 5472 5472 Size: 5596 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2085 2095 4710 4894 1305 Size: 780 10 2615 184 2007 Fragments arranged by size: 2615 2007 780 184 10 MscI TGG'CCA Cuts at: 10 64 1489 1832 2204 10 Size: 54 1425 343 372 3402 Fragments arranged by size: 3402 1425 372 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2447 2495 2498 3554 3913 4072 518 Size: 648 1281 48 3 1056 359 159 2042 Fragments arranged by size: 2042 1281 1056 648 359 159 48 3 MspA1I CmG'CkG Cuts at: 1606 2554 3012 3689 4837 5082 1606 Size: 948 458 677 1148 245 2120 Fragments arranged by size: 2120 1148 948 677 458 245 NcoI C'CATG_G Cuts at: 513 1065 2496 513 Size: 552 1431 3613 Fragments arranged by size: 3613 1431 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5596 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5596 NotI GC'GGCC_GC Cuts at: 3043 3043 Size: 5596 NspI r_CATG'y Cuts at: 1132 1229 2593 2770 2794 2954 4499 1132 Size: 97 1364 177 24 160 1545 2229 Fragments arranged by size: 2229 1545 1364 177 160 97 24 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5596 PciI A'CATG_T Cuts at: 2589 2766 4495 2589 Size: 177 1729 3690 Fragments arranged by size: 3690 1729 177 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5596 PmeI GTTT'AAAC Cuts at: 2900 2900 Size: 5596 PmlI CAC'GTG Cuts at: 2288 2288 Size: 5596 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3665 3697 5178 5210 3665 Size: 32 1481 32 4051 Fragments arranged by size: 4051 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5596 PsiI TTA'TAA Cuts at: 3166 3166 Size: 5596 PspOMI G'GGCC_C Cuts at: 2700 2700 Size: 5596 PstI C_TGCA'G Cuts at: 830 2885 2948 830 Size: 2055 63 3478 Fragments arranged by size: 3478 2055 63 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5596 RsrII CG'GwC_CG Cuts at: 2383 2383 Size: 5596 SacI G_AGCT'C Cuts at: 2853 2853 Size: 5596 SalI G'TCGA_C Cuts at: 1936 2938 1936 Size: 1002 4594 SbfI CC_TGCA'GG Cuts at: 2948 2948 Size: 5596 ScaI AGT'ACT Cuts at: 3844 5556 3844 Size: 1712 3884 SfcI C'TryA_G Cuts at: 826 1045 2194 2881 2944 4079 4760 4951 826 Size: 219 1149 687 63 1135 681 191 1471 Fragments arranged by size: 1471 1149 1135 687 681 219 191 63 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5596 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5596 SmaI CCC'GGG Cuts at: 1872 2923 1872 Size: 1051 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2279 3700 4601 4863 820 Size: 197 926 336 1421 901 262 1553 Fragments arranged by size: 1553 1421 926 901 336 262 197 SnaBI TAC'GTA Cuts at: 493 3387 493 Size: 2894 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5596 SphI G_CATG'C Cuts at: 1132 2954 1132 Size: 1822 3774 SspI AAT'ATT Cuts at: 5 52 3520 5 Size: 47 3468 2081 Fragments arranged by size: 3468 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5596 StyI C'CwwG_G Cuts at: 513 1065 2496 3035 513 Size: 552 1431 539 3074 Fragments arranged by size: 3074 1431 552 539 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2191 3623 3640 3793 3978 4423 325 Size: 1255 611 1432 17 153 185 445 1498 Fragments arranged by size: 1498 1432 1255 611 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2181 3842 5554 Size: 274 80 33 51 1443 204 1661 1712 Cuts at: 5554 96 Size: 138 Fragments arranged by size: 1712 1661 1443 274 204 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2655 2687 5556 5588 2655 Size: 32 2869 32 2663 Fragments arranged by size: 2869 2663 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5596 XbaI T'CTAG_A Cuts at: 2932 2932 Size: 5596 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5596 XmaI C'CCGG_G Cuts at: 1870 2921 1870 Size: 1051 4545 XmnI GAAnn'nnTTC Cuts at: 2056 3725 2056 Size: 1669 3927 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3404 276 Size: 53 83 186 936 1870 2468 Fragments arranged by size: 2468 1870 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI RsrII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspXI PsrI PvuII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI