(Circular) (Six-base) MAPSORT of: pFN21AB8785.seq Check: 8887 from: 1 to: 5584 pFN21AB8785 5584 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2279 2279 Size: 5584 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3394 278 Size: 53 83 186 936 1858 2468 Fragments arranged by size: 2468 1858 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2927 1937 Size: 990 4594 Acc65I G'GTAC_C Cuts at: 2905 2905 Size: 5584 AclI AA'CG_TT Cuts at: 1563 2016 3711 4084 1563 Size: 453 1695 373 3063 Fragments arranged by size: 3063 1695 453 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2528 3647 5031 5299 1632 Size: 896 1119 1384 268 1917 Fragments arranged by size: 1917 1384 1119 896 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5387 AflIII A'CryG_T Cuts at: 1203 2389 4483 5460 1203 Size: 1186 2094 977 1327 Fragments arranged by size: 2094 1327 1186 977 AgeI A'CCGG_T Cuts at: 4415 4415 Size: 5584 AhdI GACnn_n'nnGTC Cuts at: 4313 4313 Size: 5584 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5584 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5584 AlwNI CAG_nnn'CTG Cuts at: 3327 4899 3327 Size: 1572 4012 ApaLI G'TGCA_C Cuts at: 3641 4797 3641 Size: 1156 4428 ApoI r'AATT_y Cuts at: 1493 2893 3119 3271 5380 1493 Size: 1400 226 152 2109 1697 Fragments arranged by size: 2109 1697 1400 226 152 AseI AT'TA_AT Cuts at: 160 1032 4138 160 Size: 872 3106 1606 Fragments arranged by size: 3106 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5584 AvaI C'yCGr_G Cuts at: 1870 1943 2193 2909 1870 Size: 73 250 716 4545 Fragments arranged by size: 4545 716 250 73 BamHI G'GATC_C Cuts at: 2032 2914 3281 2032 Size: 882 367 4335 Fragments arranged by size: 4335 882 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2905 4360 618 Size: 325 217 627 1118 1455 1842 Fragments arranged by size: 1842 1455 1118 627 325 217 BanII G_rGCy'C Cuts at: 1391 2903 1391 Size: 1512 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2745 928 Size: 971 846 3767 Fragments arranged by size: 3767 971 846 BbvCI CC'TCA_GC Cuts at: 2748 2835 2748 Size: 87 5497 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3775 3809 1903 Size: 34 1838 34 3678 Fragments arranged by size: 3678 1838 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3476 4686 3476 Size: 1210 4374 BclI T'GATC_A Cuts at: 1548 2811 1548 Size: 1263 4321 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4195 136 Size: 107 122 71 631 605 2523 1525 Fragments arranged by size: 2523 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5579 5579 Size: 5584 BlpI GC'TnA_GC Cuts at: 3001 3001 Size: 5584 Bme1580I G_kGCm'C Cuts at: 1790 2430 3645 4801 1790 Size: 640 1215 1156 2573 Fragments arranged by size: 2573 1215 1156 640 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5584 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3315 4273 442 Size: 416 53 83 399 24 1898 958 1753 Fragments arranged by size: 1898 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2235 1056 Size: 1179 4405 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2825 4244 1239 Size: 1586 1419 2579 Fragments arranged by size: 2579 1586 1419 Bpu10I CC'TnA_GC Cuts at: 2748 2835 2748 Size: 87 5497 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3709 4574 4872 3709 Size: 865 298 4421 Fragments arranged by size: 4421 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4247 882 Size: 617 2748 2219 Fragments arranged by size: 2748 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2390 3375 493 Size: 711 1186 985 2702 Fragments arranged by size: 2702 1186 985 711 BsaBI GATnn'nnATC Cuts at: 5578 5578 Size: 5584 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3391 3773 4408 275 Size: 53 83 186 936 1858 382 635 1451 Fragments arranged by size: 1858 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4017 4415 4689 4836 5247 4017 Size: 398 274 147 411 4354 Fragments arranged by size: 4354 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5359 Fragments arranged by size: 5359 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2314 2388 2391 2394 2684 2765 1186 Size: 1128 74 3 3 290 81 4005 Fragments arranged by size: 4005 1128 290 81 74 3 3 BseYI C'CCAG_C Cuts at: 2383 4787 5283 2383 Size: 2404 496 2684 Fragments arranged by size: 2684 2404 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2754 1163 Size: 573 1018 3993 Fragments arranged by size: 3993 1018 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3034 3795 4823 1540 Size: 275 186 1033 761 1028 2301 Fragments arranged by size: 2301 1033 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3645 3730 4801 3645 Size: 85 1071 4428 Fragments arranged by size: 4428 1071 85 BsmI GAATG_Cn' Cuts at: 3095 3188 3095 Size: 93 5491 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2430 2903 3645 3730 4801 1391 Size: 399 640 473 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 640 473 399 85 BspEI T'CCGG_A Cuts at: 5247 5247 Size: 5584 BspHI T'CATG_A Cuts at: 3473 5203 3473 Size: 1730 3854 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2279 2939 844 Size: 1435 660 3489 Fragments arranged by size: 3489 1435 660 BsrBI CCG'CTC Cuts at: 1394 1823 3471 1394 Size: 429 1648 3507 Fragments arranged by size: 3507 1648 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2409 4079 4253 65 Size: 1183 1161 1670 174 1396 Fragments arranged by size: 1670 1396 1183 1161 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4228 4415 1700 Size: 110 2418 187 2869 Fragments arranged by size: 2869 2418 187 110 BsrGI T'GTAC_A Cuts at: 96 2373 96 Size: 2277 3307 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5584 BssSI C'ACGA_G Cuts at: 3644 4656 3644 Size: 1012 4572 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5584 BstBI TT'CG_AA Cuts at: 4388 4388 Size: 5584 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5584 BstXI CCAn_nnnn'nTGG Cuts at: 2029 2416 2029 Size: 387 5197 BstYI r'GATC_y Cuts at: 1972 2032 2507 2914 3281 3666 3683 5135 5221 Size: 60 475 407 367 385 17 1452 86 Cuts at: 5221 5233 5579 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 475 407 385 367 346 86 60 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4093 Fragments arranged by size: 4093 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4845 Fragments arranged by size: 4845 683 56 BtsI GCAGTG_nn' Cuts at: 3100 3894 3914 3100 Size: 794 20 4770 Fragments arranged by size: 4770 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5584 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5549 DraI TTT'AAA Cuts at: 2888 2888 Size: 5584 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4591 809 Size: 507 3275 1802 Fragments arranged by size: 3275 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2869 3031 Size: 54 1425 50 275 18 177 862 162 Cuts at: 3031 3920 5549 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 862 275 177 162 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3031 1537 Size: 275 1219 4090 Fragments arranged by size: 4090 1219 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2661 3514 1353 Size: 1308 853 3423 Fragments arranged by size: 3423 1308 853 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4171 4545 4691 387 Size: 1064 138 2582 374 146 1280 Fragments arranged by size: 2582 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2528 2825 3647 4244 5031 5299 1239 Size: 393 896 297 822 597 787 268 1524 Fragments arranged by size: 1524 896 822 787 597 393 297 268 EcoNI CCTnn'n_nnAGG Cuts at: 2464 2464 Size: 5584 EcoO109I rG'GnC_Cy Cuts at: 2758 2758 Size: 5584 EcoRI G'AATT_C Cuts at: 2893 3271 2893 Size: 378 5206 FspI TGC'GCA Cuts at: 4090 4090 Size: 5584 HaeII r_GCGC'y Cuts at: 1678 4731 1678 Size: 3053 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2020 2024 2052 2056 4225 Size: 32 135 32 448 4 28 4 2169 Cuts at: 4225 4257 4299 4331 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2169 448 135 42 32 32 32 32 28 4 4 HincII GTy'rAC Cuts at: 669 1657 1938 2441 2565 2928 3174 669 Size: 988 281 503 124 363 246 3079 Fragments arranged by size: 3079 988 503 363 281 246 124 HindIII A'AGCT_T Cuts at: 748 748 Size: 5584 HpaI GTT'AAC Cuts at: 3174 3174 Size: 5584 KpnI G_GTAC'C Cuts at: 2909 2909 Size: 5584 MfeI C'AATT_G Cuts at: 3183 3183 Size: 5584 MluI A'CGCG_T Cuts at: 5460 5460 Size: 5584 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4698 4882 1305 Size: 3393 184 2007 Fragments arranged by size: 3393 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2871 10 Size: 54 1425 343 177 862 2723 Fragments arranged by size: 2723 1425 862 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 3542 3901 4060 518 Size: 648 2376 359 159 2042 Fragments arranged by size: 2376 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2309 2803 3000 3677 4825 5070 1606 Size: 703 494 197 677 1148 245 2120 Fragments arranged by size: 2120 1148 703 677 494 245 197 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4093 Fragments arranged by size: 4093 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5584 NheI G'CTAG_C Cuts at: 1052 2231 1052 Size: 1179 4405 NotI GC'GGCC_GC Cuts at: 3031 3031 Size: 5584 NspI r_CATG'y Cuts at: 1132 1229 2942 4487 1132 Size: 97 1713 1545 2229 Fragments arranged by size: 2229 1713 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5584 PciI A'CATG_T Cuts at: 4483 4483 Size: 5584 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5584 PfoI T'CCnGG_A Cuts at: 2595 2595 Size: 5584 PmeI GTTT'AAAC Cuts at: 2888 2888 Size: 5584 PmlI CAC'GTG Cuts at: 2390 2390 Size: 5584 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3653 3685 5166 5198 3653 Size: 32 1481 32 4039 Fragments arranged by size: 4039 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2758 2758 Size: 5584 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5584 PsiI TTA'TAA Cuts at: 3154 3154 Size: 5584 PstI C_TGCA'G Cuts at: 830 2936 830 Size: 2106 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5584 PvuII CAG'CTG Cuts at: 2803 2803 Size: 5584 SalI G'TCGA_C Cuts at: 1936 2926 1936 Size: 990 4594 SanDI GG'GwC_CC Cuts at: 2758 2758 Size: 5584 SbfI CC_TGCA'GG Cuts at: 2936 2936 Size: 5584 ScaI AGT'ACT Cuts at: 3832 5544 3832 Size: 1712 3872 SfcI C'TryA_G Cuts at: 826 1045 2202 2421 2932 4067 4748 4939 826 Size: 219 1157 219 511 1135 681 191 1471 Fragments arranged by size: 1471 1157 1135 681 511 219 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5584 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5584 SmaI CCC'GGG Cuts at: 1872 2195 2911 1872 Size: 323 716 4545 Fragments arranged by size: 4545 716 323 SmlI C'TyrA_G Cuts at: 820 1017 1943 3688 4589 4851 820 Size: 197 926 1745 901 262 1553 Fragments arranged by size: 1745 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3375 493 Size: 2882 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5584 SphI G_CATG'C Cuts at: 1132 2942 1132 Size: 1810 3774 SrfI GCCC'GGGC Cuts at: 2195 2195 Size: 5584 SspI AAT'ATT Cuts at: 5 52 3508 5 Size: 47 3456 2081 Fragments arranged by size: 3456 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5584 StyI C'CwwG_G Cuts at: 513 1065 2004 3023 513 Size: 552 939 1019 3074 Fragments arranged by size: 3074 1019 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3611 3628 3781 3966 4411 325 Size: 1255 2031 17 153 185 445 1498 Fragments arranged by size: 2031 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2373 3830 5542 Size: 274 80 33 51 1443 396 1457 1712 Cuts at: 5542 96 Size: 138 Fragments arranged by size: 1712 1457 1443 396 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5544 5576 5544 Size: 32 5552 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5584 XbaI T'CTAG_A Cuts at: 2920 2920 Size: 5584 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5584 XmaI C'CCGG_G Cuts at: 1870 2193 2909 1870 Size: 323 716 4545 Fragments arranged by size: 4545 716 323 XmnI GAAnn'nnTTC Cuts at: 3713 3713 Size: 5584 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3392 276 Size: 53 83 186 936 1858 2468 Fragments arranged by size: 2468 1858 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SwaI XcmI