Restriction map for
pFN21AB8785
(Linear) (Six-base) MAPSORT of: KIBB8785.seq Check: 7628 from: 1 to: 879
KIBB8785.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 274 879
Size: 274 605
AclI AA'CG_TT
Cuts at: 0 11 879
Size: 11 868
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 523 879
Size: 523 356
AflIII A'CryG_T
Cuts at: 0 384 879
Size: 384 495
AvaI C'yCGr_G
Cuts at: 0 188 879
Size: 188 691
BamHI G'GATC_C
Cuts at: 0 27 879
Size: 27 852
BbsI GAAGACnn'nnnn_
Cuts at: 0 740 879
Size: 740 139
BbvCI CC'TCA_GC
Cuts at: 0 743 830 879
Size: 743 87 49
Fragments arranged by size:
743 87 49
BclI T'GATC_A
Cuts at: 0 806 879
Size: 806 73
Bme1580I G_kGCm'C
Cuts at: 0 425 879
Size: 425 454
BmtI G_CTAG'C
Cuts at: 0 230 879
Size: 230 649
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 820 879
Size: 820 59
Bpu10I CC'TnA_GC
Cuts at: 0 743 830 879
Size: 743 87 49
Fragments arranged by size:
743 87 49
BsaAI yAC'GTr
Cuts at: 0 385 879
Size: 385 494
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 309 383 386 389 679 760 879
Size: 309 74 3 3 290 81 119
Fragments arranged by size:
309 290 119 81 74 3 3
BseYI C'CCAG_C
Cuts at: 0 378 879
Size: 378 501
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 749 879
Size: 749 130
Bsp1286I G_dGCh'C
Cuts at: 0 425 879
Size: 425 454
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 274 879
Size: 274 605
BsrDI GCAATG_nn'
Cuts at: 0 404 879
Size: 404 475
BsrGI T'GTAC_A
Cuts at: 0 368 879
Size: 368 511
BstXI CCAn_nnnn'nTGG
Cuts at: 0 24 411 879
Size: 24 387 468
Fragments arranged by size:
468 387 24
BstYI r'GATC_y
Cuts at: 0 27 502 879
Size: 27 475 377
Fragments arranged by size:
475 377 27
EaeI y'GGCC_r
Cuts at: 0 2 864 879
Size: 2 862 15
Fragments arranged by size:
862 15 2
EarI CTCTTCn'nnn_
Cuts at: 0 656 879
Size: 656 223
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 523 820 879
Size: 523 297 59
Fragments arranged by size:
523 297 59
EcoNI CCTnn'n_nnAGG
Cuts at: 0 459 879
Size: 459 420
EcoO109I rG'GnC_Cy
Cuts at: 0 753 879
Size: 753 126
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 15 19 47 51 879
Size: 15 4 28 4 828
Fragments arranged by size:
828 28 15 4 4
HincII GTy'rAC
Cuts at: 0 436 560 879
Size: 436 124 319
Fragments arranged by size:
436 319 124
MscI TGG'CCA
Cuts at: 0 4 866 879
Size: 4 862 13
Fragments arranged by size:
862 13 4
MspA1I CmG'CkG
Cuts at: 0 304 798 879
Size: 304 494 81
Fragments arranged by size:
494 304 81
NheI G'CTAG_C
Cuts at: 0 226 879
Size: 226 653
PfoI T'CCnGG_A
Cuts at: 0 590 879
Size: 590 289
PmlI CAC'GTG
Cuts at: 0 385 879
Size: 385 494
PpuMI rG'GwC_Cy
Cuts at: 0 753 879
Size: 753 126
PvuII CAG'CTG
Cuts at: 0 798 879
Size: 798 81
SanDI GG'GwC_CC
Cuts at: 0 753 879
Size: 753 126
SfcI C'TryA_G
Cuts at: 0 197 416 879
Size: 197 219 463
Fragments arranged by size:
463 219 197
SmaI CCC'GGG
Cuts at: 0 190 879
Size: 190 689
SrfI GCCC'GGGC
Cuts at: 0 190 879
Size: 190 689
TatI w'GTAC_w
Cuts at: 0 368 879
Size: 368 511
XmaI C'CCGG_G
Cuts at: 0 188 879
Size: 188 691
Enzymes that do cut:
AarI AclI AcuI AflIII AvaI BamHI BbsI BbvCI
BclI Bme1580I BmtI BpmI Bpu10I BsaAI BseRI BseYI
BsgI Bsp1286I BspMI BsrDI BsrGI BstXI BstYI EaeI
EarI Eco57MI EcoNI EcoO109I Hin4I HincII MscI MspA1I
NheI PfoI PmlI PpuMI PvuII SanDI SfcI SmaI
SrfI TatI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AfeI AflII AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BanI BanII BbeI BcgI BciVI
BfrBI BglI BglII BlpI BmgBI BmrI BplI BpuEI
BsaI BsaBI BsaHI BsaWI BsaXI BsiEI BsiHKAI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HindIII
HpaI KasI KpnI MfeI MluI MmeI MslI NaeI
NarI NcoI NdeI NgoMIV NotI NruI NsiI NspI
PacI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmlI SnaBI SpeI SphI SspI StuI StyI
SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmnI
ZraI