(Circular) (Six-base) MAPSORT of: pFN21AB8782.seq Check: 1977 from: 1 to: 5524 pFN21AB8782 5524 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3334 278 Size: 53 83 186 936 1798 2468 Fragments arranged by size: 2468 1798 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2867 1937 Size: 930 4594 Acc65I G'GTAC_C Cuts at: 2111 2845 2111 Size: 734 4790 AclI AA'CG_TT Cuts at: 1563 3651 4024 1563 Size: 2088 373 3063 Fragments arranged by size: 3063 2088 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3587 4971 5239 1632 Size: 1955 1384 268 1917 Fragments arranged by size: 1955 1917 1384 268 AfeI AGC'GCT Cuts at: 2720 2720 Size: 5524 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5327 AflIII A'CryG_T Cuts at: 1203 2535 4423 5400 1203 Size: 1332 1888 977 1327 Fragments arranged by size: 1888 1332 1327 977 AgeI A'CCGG_T Cuts at: 4355 4355 Size: 5524 AhdI GACnn_n'nnGTC Cuts at: 4253 4253 Size: 5524 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5524 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5524 AlwNI CAG_nnn'CTG Cuts at: 3267 4839 3267 Size: 1572 3952 ApaLI G'TGCA_C Cuts at: 2222 2399 3581 4737 2222 Size: 177 1182 1156 3009 Fragments arranged by size: 3009 1182 1156 177 ApoI r'AATT_y Cuts at: 1493 2833 3059 3211 5320 1493 Size: 1340 226 152 2109 1697 Fragments arranged by size: 2109 1697 1340 226 152 AseI AT'TA_AT Cuts at: 160 1032 4078 160 Size: 872 3046 1606 Fragments arranged by size: 3046 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5524 AvaI C'yCGr_G Cuts at: 1870 1943 2543 2705 2849 1870 Size: 73 600 162 144 4545 Fragments arranged by size: 4545 600 162 144 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2095 2128 2095 Size: 33 5491 BamHI G'GATC_C Cuts at: 2854 3221 2854 Size: 367 5157 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2111 2366 2670 2845 4300 Size: 325 217 627 324 255 304 175 1455 Cuts at: 4300 618 Size: 1842 Fragments arranged by size: 1842 1455 627 325 324 304 255 217 175 BanII G_rGCy'C Cuts at: 1391 2438 2843 1391 Size: 1047 405 4072 Fragments arranged by size: 4072 1047 405 BbeI G_GCGC'C Cuts at: 2370 2674 2370 Size: 304 5220 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2221 928 Size: 971 322 4231 Fragments arranged by size: 4231 971 322 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2333 2367 2584 2618 2620 2654 3715 Size: 34 396 34 217 34 2 34 1061 Cuts at: 3715 3749 1903 Size: 34 3678 Fragments arranged by size: 3678 1061 396 217 34 34 34 34 34 2 BciVI GTATCCnnnnn_n' Cuts at: 3416 4626 3416 Size: 1210 4314 BclI T'GATC_A Cuts at: 1548 1548 Size: 5524 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4135 136 Size: 107 122 71 631 605 2463 1525 Fragments arranged by size: 2463 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5519 5519 Size: 5524 BlpI GC'TnA_GC Cuts at: 2432 2524 2941 2432 Size: 92 417 5015 Fragments arranged by size: 5015 417 92 Bme1580I G_kGCm'C Cuts at: 1790 2226 2403 3585 4741 1790 Size: 436 177 1182 1156 2573 Fragments arranged by size: 2573 1182 1156 436 177 BmgBI CAC'GTC Cuts at: 1318 2227 1318 Size: 909 4615 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3255 4213 442 Size: 416 53 83 399 24 1838 958 1753 Fragments arranged by size: 1838 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5524 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4184 1239 Size: 2945 2579 Bpu10I CC'TnA_GC Cuts at: 2504 2504 Size: 5524 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2396 3649 4514 4812 2396 Size: 1253 865 298 3108 Fragments arranged by size: 3108 1253 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4187 882 Size: 617 2688 2219 Fragments arranged by size: 2688 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3315 493 Size: 711 2111 2702 Fragments arranged by size: 2702 2111 711 BsaBI GATnn'nnATC Cuts at: 5518 5518 Size: 5524 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2367 2601 2671 3331 Size: 53 83 186 936 834 234 70 660 Cuts at: 3331 3713 4348 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 834 660 635 382 234 186 83 70 53 BsaWI w'CCGG_w Cuts at: 3957 4355 4629 4776 5187 3957 Size: 398 274 147 411 4294 Fragments arranged by size: 4294 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5299 Fragments arranged by size: 5299 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5524 BseYI C'CCAG_C Cuts at: 2044 4727 5223 2044 Size: 2683 496 2345 Fragments arranged by size: 2683 2345 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2327 2366 1163 Size: 573 591 39 4321 Fragments arranged by size: 4321 591 573 39 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2283 2479 2974 3735 4763 1540 Size: 275 186 282 196 495 761 1028 2301 Fragments arranged by size: 2301 1028 761 495 282 275 196 186 BsiHKAI G_wGCw'C Cuts at: 2226 2403 2510 2553 2610 2691 3585 3670 4741 Size: 177 107 43 57 81 894 85 1071 Cuts at: 4741 2226 Size: 3009 Fragments arranged by size: 3009 1071 894 177 107 85 81 57 43 BsmI GAATG_Cn' Cuts at: 3035 3128 3035 Size: 93 5431 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2226 2403 2438 2510 2553 2610 2691 Size: 399 436 177 35 72 43 57 81 Cuts at: 2691 2843 3585 3670 4741 1391 Size: 152 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 436 399 177 152 85 81 72 57 43 35 BspEI T'CCGG_A Cuts at: 5187 5187 Size: 5524 BspHI T'CATG_A Cuts at: 3413 5143 3413 Size: 1730 3794 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2301 2388 2879 844 Size: 1457 87 491 3489 Fragments arranged by size: 3489 1457 491 87 BsrBI CCG'CTC Cuts at: 1394 1823 3411 1394 Size: 429 1588 3507 Fragments arranged by size: 3507 1588 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4019 4193 65 Size: 1183 2771 174 1396 Fragments arranged by size: 2771 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2136 2363 2657 4168 4355 1700 Size: 110 326 227 294 1511 187 2869 Fragments arranged by size: 2869 1511 326 294 227 187 110 BsrGI T'GTAC_A Cuts at: 96 2262 96 Size: 2166 3358 BssHII G'CGCG_C Cuts at: 1925 2339 2789 2806 1925 Size: 414 450 17 4643 Fragments arranged by size: 4643 450 414 17 BssSI C'ACGA_G Cuts at: 3584 4596 3584 Size: 1012 4512 BstAPI GCAn_nnn'nTGC Cuts at: 794 2221 2305 794 Size: 1427 84 4013 Fragments arranged by size: 4013 1427 84 BstBI TT'CG_AA Cuts at: 4328 4328 Size: 5524 BstEII G'GTnAC_C Cuts at: 1184 2620 1184 Size: 1436 4088 BstXI CCAn_nnnn'nTGG Cuts at: 2128 2489 2128 Size: 361 5163 BstYI r'GATC_y Cuts at: 1972 2854 3221 3606 3623 5075 5161 5173 5519 Size: 882 367 385 17 1452 86 12 346 Cuts at: 5519 1972 Size: 1977 Fragments arranged by size: 1977 1452 882 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2115 2488 2775 513 Size: 552 1050 373 287 3262 Fragments arranged by size: 3262 1050 552 373 287 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2638 487 Size: 683 56 1412 3373 Fragments arranged by size: 3373 1412 683 56 BtsI GCAGTG_nn' Cuts at: 3040 3834 3854 3040 Size: 794 20 4710 Fragments arranged by size: 4710 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5524 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5489 DraI TTT'AAA Cuts at: 2828 2828 Size: 5524 DraIII CAC_nnn'GTG Cuts at: 2221 2398 2628 2221 Size: 177 230 5117 Fragments arranged by size: 5117 230 177 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4531 809 Size: 507 3215 1802 Fragments arranged by size: 3215 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2088 2118 2280 Size: 54 1425 50 275 18 258 30 162 Cuts at: 2280 2476 2971 3860 5489 8 Size: 196 495 889 1629 43 Fragments arranged by size: 1629 1425 889 495 275 258 196 162 54 50 43 30 18 EagI C'GGCC_G Cuts at: 1537 1812 2280 2476 2971 1537 Size: 275 468 196 495 4090 Fragments arranged by size: 4090 495 468 275 196 EarI CTCTTCn'nnn_ Cuts at: 1353 3454 1353 Size: 2101 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2752 4111 4485 4631 387 Size: 1064 138 1163 1359 374 146 1280 Fragments arranged by size: 1359 1280 1163 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3587 4184 4971 5239 1239 Size: 393 1955 597 787 268 1524 Fragments arranged by size: 1955 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2728 2769 2728 Size: 41 5483 EcoRI G'AATT_C Cuts at: 2833 3211 2833 Size: 378 5146 FspI TGC'GCA Cuts at: 2355 4030 2355 Size: 1675 3849 HaeII r_GCGC'y Cuts at: 1678 2150 2370 2449 2674 2722 4671 1678 Size: 472 220 79 225 48 1949 2531 Fragments arranged by size: 2531 1949 472 225 220 79 48 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4165 4197 4239 4271 1373 Size: 32 135 32 2593 32 42 32 2626 Fragments arranged by size: 2626 2593 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2164 2868 3114 669 Size: 988 281 226 704 246 3079 Fragments arranged by size: 3079 988 704 281 246 226 HindIII A'AGCT_T Cuts at: 748 748 Size: 5524 HpaI GTT'AAC Cuts at: 3114 3114 Size: 5524 KasI G'GCGC_C Cuts at: 2366 2670 2366 Size: 304 5220 KpnI G_GTAC'C Cuts at: 2115 2849 2115 Size: 734 4790 MfeI C'AATT_G Cuts at: 2055 3123 2055 Size: 1068 4456 MluI A'CGCG_T Cuts at: 2535 5400 2535 Size: 2865 2659 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4638 4822 1305 Size: 3333 184 2007 Fragments arranged by size: 3333 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2090 2120 10 Size: 54 1425 343 258 30 3414 Fragments arranged by size: 3414 1425 343 258 54 30 MslI CAynn'nnrTG Cuts at: 518 1166 2126 2243 2270 3482 3841 4000 518 Size: 648 960 117 27 1212 359 159 2042 Fragments arranged by size: 2042 1212 960 648 359 159 117 27 MspA1I CmG'CkG Cuts at: 1606 2777 2940 3617 4765 5010 1606 Size: 1171 163 677 1148 245 2120 Fragments arranged by size: 2120 1171 1148 677 245 163 NaeI GCC'GGC Cuts at: 2365 2659 2365 Size: 294 5230 NarI GG'CG_CC Cuts at: 2367 2671 2367 Size: 304 5220 NcoI C'CATG_G Cuts at: 513 1065 2488 513 Size: 552 1423 3549 Fragments arranged by size: 3549 1423 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5524 NgoMIV G'CCGG_C Cuts at: 2363 2657 2363 Size: 294 5230 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5524 NotI GC'GGCC_GC Cuts at: 2280 2971 2280 Size: 691 4833 NspI r_CATG'y Cuts at: 1132 1229 2635 2882 4427 1132 Size: 97 1406 247 1545 2229 Fragments arranged by size: 2229 1545 1406 247 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5524 PciI A'CATG_T Cuts at: 4423 4423 Size: 5524 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5524 PmeI GTTT'AAAC Cuts at: 2828 2828 Size: 5524 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3593 3625 5106 5138 3593 Size: 32 1481 32 3979 Fragments arranged by size: 3979 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5524 PsiI TTA'TAA Cuts at: 2040 3094 2040 Size: 1054 4470 PspXI vC'TCGA_Gb Cuts at: 2705 2705 Size: 5524 PstI C_TGCA'G Cuts at: 830 2751 2876 830 Size: 1921 125 3478 Fragments arranged by size: 3478 1921 125 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5524 SacII CC_GC'GG Cuts at: 2778 2778 Size: 5524 SalI G'TCGA_C Cuts at: 1936 2866 1936 Size: 930 4594 SbfI CC_TGCA'GG Cuts at: 2876 2876 Size: 5524 ScaI AGT'ACT Cuts at: 3772 5484 3772 Size: 1712 3812 SexAI A'CCwGG_T Cuts at: 2394 2394 Size: 5524 SfcI C'TryA_G Cuts at: 826 1045 2747 2872 4007 4688 4879 826 Size: 219 1702 125 1135 681 191 1471 Fragments arranged by size: 1702 1471 1135 681 219 191 125 SfoI GGC'GCC Cuts at: 2368 2672 2368 Size: 304 5220 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5524 SgrAI Cr'CCGG_yG Cuts at: 1700 2363 1700 Size: 663 4861 SmaI CCC'GGG Cuts at: 1872 2851 1872 Size: 979 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2411 2705 3628 4529 4791 820 Size: 197 926 468 294 923 901 262 1553 Fragments arranged by size: 1553 926 923 901 468 294 262 197 SnaBI TAC'GTA Cuts at: 493 3315 493 Size: 2822 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5524 SphI G_CATG'C Cuts at: 1132 2635 2882 1132 Size: 1503 247 3774 Fragments arranged by size: 3774 1503 247 SspI AAT'ATT Cuts at: 5 52 3448 5 Size: 47 3396 2081 Fragments arranged by size: 3396 2081 47 StuI AGG'CCT Cuts at: 1517 2703 1517 Size: 1186 4338 StyI C'CwwG_G Cuts at: 513 1065 2076 2488 2963 513 Size: 552 1011 412 475 3074 Fragments arranged by size: 3074 1011 552 475 412 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3551 3568 3721 3906 4351 325 Size: 1255 1971 17 153 185 445 1498 Fragments arranged by size: 1971 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2103 2262 3770 Size: 274 80 33 51 1443 126 159 1508 Cuts at: 3770 5482 96 Size: 1712 138 Fragments arranged by size: 1712 1508 1443 274 159 138 126 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5484 5516 5484 Size: 32 5492 Tth111I GACn'n_nGTC Cuts at: 1316 2159 1316 Size: 843 4681 XbaI T'CTAG_A Cuts at: 2860 2860 Size: 5524 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2083 2128 2083 Size: 45 5479 XhoI C'TCGA_G Cuts at: 1943 2705 1943 Size: 762 4762 XmaI C'CCGG_G Cuts at: 1870 2849 1870 Size: 979 4545 XmnI GAAnn'nnTTC Cuts at: 3653 3653 Size: 5524 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3332 276 Size: 53 83 186 936 1798 2468 Fragments arranged by size: 2468 1798 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PsrI PvuII RsrII SacI SanDI SapI SfiI SrfI SwaI