(Circular) (Six-base) MAPSORT of: pFN21AB8763.seq Check: 5094 from: 1 to: 5338 pFN21AB8763 5338 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3148 278 Size: 53 83 186 936 1612 2468 Fragments arranged by size: 2468 1612 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2681 1937 Size: 744 4594 Acc65I G'GTAC_C Cuts at: 2659 2659 Size: 5338 AclI AA'CG_TT Cuts at: 1563 3465 3838 1563 Size: 1902 373 3063 Fragments arranged by size: 3063 1902 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3401 4785 5053 1632 Size: 1769 1384 268 1917 Fragments arranged by size: 1917 1769 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5141 AflIII A'CryG_T Cuts at: 1203 4237 5214 1203 Size: 3034 977 1327 Fragments arranged by size: 3034 1327 977 AgeI A'CCGG_T Cuts at: 4169 4169 Size: 5338 AhdI GACnn_n'nnGTC Cuts at: 4067 4067 Size: 5338 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5338 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2402 1184 Size: 1218 4120 AlwNI CAG_nnn'CTG Cuts at: 2533 3081 4653 2533 Size: 548 1572 3218 Fragments arranged by size: 3218 1572 548 ApaI G_GGCC'C Cuts at: 2253 2497 2253 Size: 244 5094 ApaLI G'TGCA_C Cuts at: 2261 2387 3395 4551 2261 Size: 126 1008 1156 3048 Fragments arranged by size: 3048 1156 1008 126 ApoI r'AATT_y Cuts at: 1493 2647 2873 3025 5134 1493 Size: 1154 226 152 2109 1697 Fragments arranged by size: 2109 1697 1154 226 152 AseI AT'TA_AT Cuts at: 160 1032 3892 160 Size: 872 2860 1606 Fragments arranged by size: 2860 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5338 AvaI C'yCGr_G Cuts at: 1870 1943 2365 2663 1870 Size: 73 422 298 4545 Fragments arranged by size: 4545 422 298 73 BamHI G'GATC_C Cuts at: 2668 3035 2668 Size: 367 4971 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2300 2473 2659 4114 618 Size: 325 217 627 513 173 186 1455 1842 Fragments arranged by size: 1842 1455 627 513 325 217 186 173 BanII G_rGCy'C Cuts at: 1391 2061 2253 2295 2497 2621 2657 1391 Size: 670 192 42 202 124 36 4072 Fragments arranged by size: 4072 670 202 192 124 42 36 BbeI G_GCGC'C Cuts at: 2477 2477 Size: 5338 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4367 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3529 3563 1903 Size: 34 1592 34 3678 Fragments arranged by size: 3678 1592 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3230 4440 3230 Size: 1210 4128 BclI T'GATC_A Cuts at: 1548 1548 Size: 5338 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2065 3949 136 Size: 107 122 71 631 605 393 1884 1525 Fragments arranged by size: 1884 1525 631 605 393 122 107 71 BglII A'GATC_T Cuts at: 5333 5333 Size: 5338 BlpI GC'TnA_GC Cuts at: 2755 2755 Size: 5338 Bme1580I G_kGCm'C Cuts at: 1790 2132 2253 2265 2303 2391 2497 3399 4555 Size: 342 121 12 38 88 106 902 1156 Cuts at: 4555 1790 Size: 2573 Fragments arranged by size: 2573 1156 902 342 121 106 88 38 12 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5338 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2254 3069 4027 Size: 416 53 83 399 24 837 815 958 Cuts at: 4027 442 Size: 1753 Fragments arranged by size: 1753 958 837 815 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5338 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2477 3998 1239 Size: 1238 1521 2579 Fragments arranged by size: 2579 1521 1238 Bpu10I CC'TnA_GC Cuts at: 2255 2348 2615 2255 Size: 93 267 4978 Fragments arranged by size: 4978 267 93 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3463 4328 4626 3463 Size: 865 298 4175 Fragments arranged by size: 4175 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4001 882 Size: 617 2502 2219 Fragments arranged by size: 2502 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2467 3129 493 Size: 711 1263 662 2702 Fragments arranged by size: 2702 1263 711 662 BsaBI GATnn'nnATC Cuts at: 5332 5332 Size: 5338 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2229 2474 3145 3527 Size: 53 83 186 936 696 245 671 382 Cuts at: 3527 4162 275 Size: 635 1451 Fragments arranged by size: 1451 936 696 671 635 382 245 186 83 53 BsaWI w'CCGG_w Cuts at: 3771 4169 4443 4590 5001 3771 Size: 398 274 147 411 4108 Fragments arranged by size: 4108 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2603 2633 1178 Size: 30 165 30 1200 30 3883 Fragments arranged by size: 3883 1200 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2074 2083 2086 1186 Size: 888 9 3 4438 Fragments arranged by size: 4438 888 9 3 BseYI C'CCAG_C Cuts at: 2488 4541 5037 2488 Size: 2053 496 2789 Fragments arranged by size: 2789 2053 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4765 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2126 2404 2522 2788 3549 4577 Size: 275 186 125 278 118 266 761 1028 Cuts at: 4577 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 278 275 266 186 125 118 BsiHKAI G_wGCw'C Cuts at: 2265 2391 2463 2621 3399 3484 4555 2265 Size: 126 72 158 778 85 1071 3048 Fragments arranged by size: 3048 1071 778 158 126 85 72 BsmI GAATG_Cn' Cuts at: 2849 2942 2849 Size: 93 5245 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2061 2132 2253 2265 2295 2303 2391 Size: 399 271 71 121 12 30 8 88 Cuts at: 2391 2463 2497 2621 2657 3399 3484 4555 1391 Size: 72 34 124 36 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 271 124 121 88 85 72 71 36 34 30 12 8 BspEI T'CCGG_A Cuts at: 5001 5001 Size: 5338 BspHI T'CATG_A Cuts at: 3227 4957 3227 Size: 1730 3608 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2693 844 Size: 1849 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2278 3225 1394 Size: 429 455 947 3507 Fragments arranged by size: 3507 947 455 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3833 4007 65 Size: 1183 2585 174 1396 Fragments arranged by size: 2585 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2196 2399 3982 4169 1700 Size: 110 386 203 1583 187 2869 Fragments arranged by size: 2869 1583 386 203 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5338 BssHII G'CGCG_C Cuts at: 1925 2114 2209 2217 2284 1925 Size: 189 95 8 67 4979 Fragments arranged by size: 4979 189 95 67 8 BssSI C'ACGA_G Cuts at: 2273 2374 3398 4410 2273 Size: 101 1024 1012 3201 Fragments arranged by size: 3201 1024 1012 101 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5338 BstBI TT'CG_AA Cuts at: 4142 4142 Size: 5338 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5338 BstYI r'GATC_y Cuts at: 1972 2668 3035 3420 3437 4889 4975 4987 5333 Size: 696 367 385 17 1452 86 12 346 Cuts at: 5333 1972 Size: 1977 Fragments arranged by size: 1977 1452 696 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2103 2120 2133 2295 2559 513 Size: 552 939 99 17 13 162 264 3292 Fragments arranged by size: 3292 939 552 264 162 99 17 13 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4599 Fragments arranged by size: 4599 683 56 BtsI GCAGTG_nn' Cuts at: 2854 3648 3668 2854 Size: 794 20 4524 Fragments arranged by size: 4524 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5338 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5303 DraI TTT'AAA Cuts at: 2642 2642 Size: 5338 DraIII CAC_nnn'GTG Cuts at: 2467 2467 Size: 5338 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2039 4345 809 Size: 507 723 2306 1802 Fragments arranged by size: 2306 1802 723 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2069 2123 2401 Size: 54 1425 50 275 18 239 54 278 Cuts at: 2401 2519 2553 2785 3674 5303 8 Size: 118 34 232 889 1629 43 Fragments arranged by size: 1629 1425 889 278 275 239 232 118 54 54 50 43 34 18 EagI C'GGCC_G Cuts at: 1537 1812 2123 2401 2519 2785 1537 Size: 275 311 278 118 266 4090 Fragments arranged by size: 4090 311 278 275 266 118 EarI CTCTTCn'nnn_ Cuts at: 1353 2092 2450 3268 1353 Size: 739 358 818 3423 Fragments arranged by size: 3423 818 739 358 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3925 4299 4445 387 Size: 1064 138 2336 374 146 1280 Fragments arranged by size: 2336 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2619 2619 Size: 5338 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2477 3401 3998 4785 5053 1239 Size: 393 845 924 597 787 268 1524 Fragments arranged by size: 1524 924 845 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2249 2250 2369 2493 2494 2249 Size: 1 119 124 1 5093 Fragments arranged by size: 5093 124 119 1 1 EcoRI G'AATT_C Cuts at: 2647 3025 2647 Size: 378 4960 FseI GG_CCGG'CC Cuts at: 2403 2403 Size: 5338 FspI TGC'GCA Cuts at: 3844 3844 Size: 5338 HaeII r_GCGC'y Cuts at: 1678 2477 2512 4485 1678 Size: 799 35 1973 2531 Fragments arranged by size: 2531 1973 799 35 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3979 4011 4053 4085 1373 Size: 32 135 32 2407 32 42 32 2626 Fragments arranged by size: 2626 2407 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2682 2928 669 Size: 988 281 744 246 3079 Fragments arranged by size: 3079 988 744 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5338 HpaI GTT'AAC Cuts at: 2928 2928 Size: 5338 KasI G'GCGC_C Cuts at: 2473 2473 Size: 5338 KpnI G_GTAC'C Cuts at: 2663 2663 Size: 5338 MfeI C'AATT_G Cuts at: 2937 2937 Size: 5338 MluI A'CGCG_T Cuts at: 5214 5214 Size: 5338 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4452 4636 1305 Size: 3147 184 2007 Fragments arranged by size: 3147 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2555 10 Size: 54 1425 343 723 2793 Fragments arranged by size: 2793 1425 723 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2384 2415 3296 3655 3814 518 Size: 648 1218 31 881 359 159 2042 Fragments arranged by size: 2042 1218 881 648 359 159 31 MspA1I CmG'CkG Cuts at: 1606 2105 2122 2135 2329 2500 2524 2754 3431 Size: 499 17 13 194 171 24 230 677 Cuts at: 3431 4579 4824 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 499 245 230 194 171 24 17 13 NaeI GCC'GGC Cuts at: 2401 2401 Size: 5338 NarI GG'CG_CC Cuts at: 2474 2474 Size: 5338 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3847 Fragments arranged by size: 3847 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5338 NgoMIV G'CCGG_C Cuts at: 2399 2399 Size: 5338 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5338 NotI GC'GGCC_GC Cuts at: 2519 2785 2519 Size: 266 5072 NspI r_CATG'y Cuts at: 1132 1229 2488 2696 4241 1132 Size: 97 1259 208 1545 2229 Fragments arranged by size: 2229 1545 1259 208 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5338 PciI A'CATG_T Cuts at: 4237 4237 Size: 5338 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5338 PfoI T'CCnGG_A Cuts at: 2223 2223 Size: 5338 PmeI GTTT'AAAC Cuts at: 2642 2642 Size: 5338 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3407 3439 4920 4952 3407 Size: 32 1481 32 3793 Fragments arranged by size: 3793 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2369 2369 Size: 5338 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5338 PsiI TTA'TAA Cuts at: 2908 2908 Size: 5338 PspOMI G'GGCC_C Cuts at: 2249 2493 2249 Size: 244 5094 PstI C_TGCA'G Cuts at: 830 2529 2690 830 Size: 1699 161 3478 Fragments arranged by size: 3478 1699 161 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5338 SacI G_AGCT'C Cuts at: 2621 2621 Size: 5338 SacII CC_GC'GG Cuts at: 2106 2123 2136 2106 Size: 17 13 5308 Fragments arranged by size: 5308 17 13 SalI G'TCGA_C Cuts at: 1936 2680 1936 Size: 744 4594 SanDI GG'GwC_CC Cuts at: 2369 2369 Size: 5338 SbfI CC_TGCA'GG Cuts at: 2690 2690 Size: 5338 ScaI AGT'ACT Cuts at: 3586 5298 3586 Size: 1712 3626 SexAI A'CCwGG_T Cuts at: 2019 2019 Size: 5338 SfcI C'TryA_G Cuts at: 826 1045 2525 2686 3821 4502 4693 826 Size: 219 1480 161 1135 681 191 1471 Fragments arranged by size: 1480 1471 1135 681 219 191 161 SfoI GGC'GCC Cuts at: 2475 2475 Size: 5338 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5338 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5338 SmaI CCC'GGG Cuts at: 1872 2665 1872 Size: 793 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3442 4343 4605 820 Size: 197 926 1499 901 262 1553 Fragments arranged by size: 1553 1499 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3129 493 Size: 2636 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5338 SphI G_CATG'C Cuts at: 1132 2488 2696 1132 Size: 1356 208 3774 Fragments arranged by size: 3774 1356 208 SspI AAT'ATT Cuts at: 5 52 3262 5 Size: 47 3210 2081 Fragments arranged by size: 3210 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5338 StyI C'CwwG_G Cuts at: 513 1065 2004 2777 513 Size: 552 939 773 3074 Fragments arranged by size: 3074 939 773 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3365 3382 3535 3720 4165 325 Size: 1255 1785 17 153 185 445 1498 Fragments arranged by size: 1785 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3584 5296 96 Size: 274 80 33 51 1443 1607 1712 138 Fragments arranged by size: 1712 1607 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5298 5330 5298 Size: 32 5306 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5338 XbaI T'CTAG_A Cuts at: 2674 2674 Size: 5338 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2221 2221 Size: 5338 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5338 XmaI C'CCGG_G Cuts at: 1870 2663 1870 Size: 793 4545 XmnI GAAnn'nnTTC Cuts at: 3467 3467 Size: 5338 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3146 276 Size: 53 83 186 936 1612 2468 Fragments arranged by size: 2468 1612 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FalI FspAI NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SapI SfiI SrfI SwaI