Restriction map for
pFN21AB8763
(Linear) (Six-base) MAPSORT of: KIBB8763.seq Check: 1269 from: 1 to: 633
KIBB8763.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 397 633
Size: 397 236
AlwNI CAG_nnn'CTG
Cuts at: 0 528 633
Size: 528 105
ApaI G_GGCC'C
Cuts at: 0 248 492 633
Size: 248 244 141
Fragments arranged by size:
248 244 141
ApaLI G'TGCA_C
Cuts at: 0 256 382 633
Size: 256 126 251
Fragments arranged by size:
256 251 126
AvaI C'yCGr_G
Cuts at: 0 360 633
Size: 360 273
BanI G'GyrC_C
Cuts at: 0 295 468 633
Size: 295 173 165
Fragments arranged by size:
295 173 165
BanII G_rGCy'C
Cuts at: 0 56 248 290 492 616 633
Size: 56 192 42 202 124 17
Fragments arranged by size:
202 192 124 56 42 17
BbeI G_GCGC'C
Cuts at: 0 472 633
Size: 472 161
BglI GCCn_nnn'nGGC
Cuts at: 0 60 633
Size: 60 573
Bme1580I G_kGCm'C
Cuts at: 0 127 248 260 298 386 492 633
Size: 127 121 12 38 88 106 141
Fragments arranged by size:
141 127 121 106 88 38 12
BmrI ACTGGGnnnn_n'
Cuts at: 0 249 633
Size: 249 384
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 472 633
Size: 472 161
Bpu10I CC'TnA_GC
Cuts at: 0 250 343 610 633
Size: 250 93 267 23
Fragments arranged by size:
267 250 93 23
BsaAI yAC'GTr
Cuts at: 0 462 633
Size: 462 171
BsaHI Gr'CG_yC
Cuts at: 0 224 469 633
Size: 224 245 164
Fragments arranged by size:
245 224 164
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 598 628 633
Size: 598 30 5
Fragments arranged by size:
598 30 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 69 78 81 633
Size: 69 9 3 552
Fragments arranged by size:
552 69 9 3
BseYI C'CCAG_C
Cuts at: 0 483 633
Size: 483 150
BsiEI CG_ry'CG
Cuts at: 0 121 399 517 633
Size: 121 278 118 116
Fragments arranged by size:
278 121 118 116
BsiHKAI G_wGCw'C
Cuts at: 0 260 386 458 616 633
Size: 260 126 72 158 17
Fragments arranged by size:
260 158 126 72 17
Bsp1286I G_dGCh'C
Cuts at: 0 56 127 248 260 290 298 386 458
Size: 56 71 121 12 30 8 88 72
Cuts at: 458 492 616 633
Size: 34 124 17
Fragments arranged by size:
124 121 88 72 71 56 34 30
17 12 8
BsrBI CCG'CTC
Cuts at: 0 273 633
Size: 273 360
BsrFI r'CCGG_y
Cuts at: 0 191 394 633
Size: 191 203 239
Fragments arranged by size:
239 203 191
BssHII G'CGCG_C
Cuts at: 0 109 204 212 279 633
Size: 109 95 8 67 354
Fragments arranged by size:
354 109 95 67 8
BssSI C'ACGA_G
Cuts at: 0 268 369 633
Size: 268 101 264
Fragments arranged by size:
268 264 101
BtgI C'CryG_G
Cuts at: 0 98 115 128 290 554 633
Size: 98 17 13 162 264 79
Fragments arranged by size:
264 162 98 79 17 13
DraIII CAC_nnn'GTG
Cuts at: 0 462 633
Size: 462 171
DrdI GACnn_nn'nnGTC
Cuts at: 0 34 633
Size: 34 599
EaeI y'GGCC_r
Cuts at: 0 64 118 396 514 548 633
Size: 64 54 278 118 34 85
Fragments arranged by size:
278 118 85 64 54 34
EagI C'GGCC_G
Cuts at: 0 118 396 514 633
Size: 118 278 118 119
Fragments arranged by size:
278 119 118 118
EarI CTCTTCn'nnn_
Cuts at: 0 87 445 633
Size: 87 358 188
Fragments arranged by size:
358 188 87
EcoICRI GAG'CTC
Cuts at: 0 614 633
Size: 614 19
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 472 633
Size: 472 161
EcoO109I rG'GnC_Cy
Cuts at: 0 244 245 364 488 489 633
Size: 244 1 119 124 1 144
Fragments arranged by size:
244 144 124 119 1 1
FseI GG_CCGG'CC
Cuts at: 0 398 633
Size: 398 235
HaeII r_GCGC'y
Cuts at: 0 472 507 633
Size: 472 35 126
Fragments arranged by size:
472 126 35
KasI G'GCGC_C
Cuts at: 0 468 633
Size: 468 165
MscI TGG'CCA
Cuts at: 0 550 633
Size: 550 83
MslI CAynn'nnrTG
Cuts at: 0 379 410 633
Size: 379 31 223
Fragments arranged by size:
379 223 31
MspA1I CmG'CkG
Cuts at: 0 100 117 130 324 495 519 633
Size: 100 17 13 194 171 24 114
Fragments arranged by size:
194 171 114 100 24 17 13
NaeI GCC'GGC
Cuts at: 0 396 633
Size: 396 237
NarI GG'CG_CC
Cuts at: 0 469 633
Size: 469 164
NgoMIV G'CCGG_C
Cuts at: 0 394 633
Size: 394 239
NotI GC'GGCC_GC
Cuts at: 0 514 633
Size: 514 119
NspI r_CATG'y
Cuts at: 0 483 633
Size: 483 150
PfoI T'CCnGG_A
Cuts at: 0 218 633
Size: 218 415
PpuMI rG'GwC_Cy
Cuts at: 0 364 633
Size: 364 269
PspOMI G'GGCC_C
Cuts at: 0 244 488 633
Size: 244 244 145
Fragments arranged by size:
244 244 145
PstI C_TGCA'G
Cuts at: 0 524 633
Size: 524 109
SacI G_AGCT'C
Cuts at: 0 616 633
Size: 616 17
SacII CC_GC'GG
Cuts at: 0 101 118 131 633
Size: 101 17 13 502
Fragments arranged by size:
502 101 17 13
SanDI GG'GwC_CC
Cuts at: 0 364 633
Size: 364 269
SexAI A'CCwGG_T
Cuts at: 0 14 633
Size: 14 619
SfcI C'TryA_G
Cuts at: 0 520 633
Size: 520 113
SfoI GGC'GCC
Cuts at: 0 470 633
Size: 470 163
SphI G_CATG'C
Cuts at: 0 483 633
Size: 483 150
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 216 633
Size: 216 417
Enzymes that do cut:
AlfI AlwNI ApaI ApaLI AvaI BanI BanII BbeI
BglI Bme1580I BmrI BpmI Bpu10I BsaAI BsaHI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BsrBI BsrFI BssHII
BssSI BtgI DraIII DrdI EaeI EagI EarI EcoICRI
Eco57MI EcoO109I FseI HaeII KasI MscI MslI MspA1I
NaeI NarI NgoMIV NotI NspI PfoI PpuMI PspOMI
PstI SacI SacII SanDI SexAI SfcI SfoI SphI
XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AloI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI BmgBI BmtI BplI BpuEI
BsaI BsaBI BsaWI BsgI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrDI BsrGI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
EciI EcoNI EcoRI EcoRV FalI FspI FspAI Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI NcoI
NdeI NheI NruI NsiI PacI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
PvuII RsrII SalI SapI SbfI ScaI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SrfI SspI StuI
StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI