Restriction map for
pFN21AB8736
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AfaI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BbrPI BbsI BbvCI
BcgI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaI BsaWI BseAI BseRI
BsiEI BsiWI BsmFI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT22I FauI FbaI
FseI FspI HgaI KpnI KspI
MaeII MamI MfeI MluI MroI
MunI MvnI NaeI NcoI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AciI 350 GCGG CCGC(-3/-1)
AcyI 659 GGCGCC G[GA]^CG[CT]C
AlwI 529 GGATC GGATC(4/5)
AlwNI 702 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 981 GAGCTC G[AT]GC[AT]^C
AvaII 69 GGACC G^G[AT]CC
BanI 659 GGCGCC G^G[CT][GA]CC
BbeI 659 GGCGCC GGCGC^C
BciVI 130 GTATCC GTATCC(6/5)
BcnI 954 CCGGG CC^[GC]GG
BfaI 127 CTAG C^TAG
BsaHI 659 GGCGCC G[GA]^CG[CT]C
BsaJI 239 CCAAGG C^C[ACGT][ACGT]GG
BsgI 829 CTGCAC GTGCAG(16/14)
BsiHKAI 981 GAGCTC G[AT]GC[AT]^C
BsmBI 818 CGTCTC CGTCTC(1/5)
BstNI 285 CCAGG CC^[AT]GG
BstYI 529 GGATCT [GA]^GATC[CT]
Cac8I 346 GCCTGC GC[ACGT]^[ACGT]GC
CfoI 660 GCGC GCG^C
EcoRII 285 CCAGG ^CC[AT]GG
EcoT14I 239 CCAAGG C^C[AT][AT]GG
HaeII 659 GGCGCC [GA]GCGC^[CT]
HapII 954 CCGG C^CGG
HhaI 660 GCGC GCG^C
Hin1I 659 GGCGCC G[GA]^CG[CT]C
HinP1I 660 GCGC G^CGC
HindIII 474 AAGCTT A^AGCTT
HpaI 499 GTTAAC GTT^AAC
HpaII 954 CCGG C^CGG
KasI 659 GGCGCC G^GCGCC
MaeI 127 CTAG C^TAG
MflI 529 GGATCT [GA]^GATC[CT]
MslI 513 CATTCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 49 CAGCTG C[AC]G^C[GT]G
MspI 954 CCGG C^CGG
MvaI 285 CCAGG CC^[AT]GG
NarI 659 GGCGCC GG^CGCC
NciI 954 CCGGG CC^[GC]GG
NdeI 140 CATATG CA^TATG
PleI 924 GACTC GAGTC(4/5)
PpuMI 68 AGGACCT [GA]G^G[AT]CC[CT]
PstI 322 CTGCAG CTGCA^G
PvuII 49 CAGCTG CAG^CTG
SacI 981 GAGCTC GAGCT^C
SapI 713 GAAGAGC GCTCTTC(1/4)
SfoI 659 GGCGCC GGC^GCC
StyI 239 CCAAGG C^C[AT][AT]GG
TaqI 331 TCGA T^CGA
TfiI 244 GAATC G^A[AT]TC
TthHB8I 331 TCGA T^CGA
XhoII 529 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BalI 110 TGGCCA TGG^CCA
360 TGGCCA
BsmAI 393 GAGAC GTCTC(1/5)
819 GTCTC
BssKI 285 CCAGG ^CC[ACGT]GG
954 CCGGG
DraII 68 AGGACCT [GA]G^G[ACGT]CC[CT]
1135 AGGGCCT
EaeI 110 TGGCCA [CT]^GGCC[GA]
360 TGGCCA
EarI 222 GAAGAG CTCTTC(1/4)
713 GAAGAG
EcoO109I 68 AGGACCT [GA]G^G[ACGT]CC[CT]
1135 AGGGCCT
EcoRI 25 GAATTC G^AATTC
550 GAATTC
HincII 411 GTCAAC GT[CT]^[GA]AC
499 GTTAAC
HindII 411 GTCAAC GT[CT]^[GA]AC
499 GTTAAC
HinfI 244 GAATC G^A[ACGT]TC
924 GACTC
HphI 84 GGTGA GGTGA(8/7)
169 GGTGA
Ksp632I 222 GAAGAG CTCTTC(1/4)
713 GAAGAG
MluNI 110 TGGCCA TGG^CCA
360 TGGCCA
MscI 110 TGGCCA TGG^CCA
360 TGGCCA
MwoI 660 GCGCCATCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1144 GCTCATACTGC
NlaIV 205 GGAGCC GG[ACGT]^[ACGT]CC
659 GGCGCC
ScrFI 285 CCAGG CC^[ACGT]GG
954 CCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AcsI AluI ApoI BanII BbvI
BmyI BpmI BsiYI BslI BsmI
Bsp1286I BsrI BstF5I Cfr13I DdeI
DpnII Fnu4HI FokI HaeIII ItaI
MaeIII MboI MboII MnlI MseI
NdeII NlaIII Sau3AI Sau96I SfaNI
SfcI Tru9I TseI Tsp45I Tsp509I
TspRI