(Circular) (Six-base) MAPSORT of: pFN21AB8736.seq Check: 2710 from: 1 to: 5905 pFN21AB8736 5905 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3715 278 Size: 53 83 186 936 2179 2468 Fragments arranged by size: 2468 2179 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3248 1937 Size: 1311 4594 Acc65I G'GTAC_C Cuts at: 3226 3226 Size: 5905 AclI AA'CG_TT Cuts at: 1563 4032 4405 1563 Size: 2469 373 3063 Fragments arranged by size: 3063 2469 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2089 2794 2962 3046 3968 5352 5620 1632 Size: 457 705 168 84 922 1384 268 1917 Fragments arranged by size: 1917 1384 922 705 457 268 168 84 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5708 AflIII A'CryG_T Cuts at: 1203 4804 5781 1203 Size: 3601 977 1327 Fragments arranged by size: 3601 1327 977 AgeI A'CCGG_T Cuts at: 4736 4736 Size: 5905 AhdI GACnn_n'nnGTC Cuts at: 4634 4634 Size: 5905 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5905 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5905 AlwNI CAG_nnn'CTG Cuts at: 2712 3648 5220 2712 Size: 936 1572 3397 Fragments arranged by size: 3397 1572 936 ApaLI G'TGCA_C Cuts at: 3962 5118 3962 Size: 1156 4749 ApoI r'AATT_y Cuts at: 1493 2030 2268 2555 3214 3440 3592 5701 1493 Size: 537 238 287 659 226 152 2109 1697 Fragments arranged by size: 2109 1697 659 537 287 238 226 152 AseI AT'TA_AT Cuts at: 160 1032 4459 160 Size: 872 3427 1606 Fragments arranged by size: 3427 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5905 AvaI C'yCGr_G Cuts at: 1870 1943 3230 1870 Size: 73 1287 4545 Fragments arranged by size: 4545 1287 73 BamHI G'GATC_C Cuts at: 3235 3602 3235 Size: 367 5538 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2664 3226 4681 618 Size: 325 217 627 877 562 1455 1842 Fragments arranged by size: 1842 1455 877 627 562 325 217 BanII G_rGCy'C Cuts at: 1391 2181 2215 2990 3224 1391 Size: 790 34 775 234 4072 Fragments arranged by size: 4072 790 775 234 34 BbeI G_GCGC'C Cuts at: 2668 2668 Size: 5905 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4934 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4096 4130 1903 Size: 34 2159 34 3678 Fragments arranged by size: 3678 2159 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2146 3797 5007 2146 Size: 1651 1210 3044 Fragments arranged by size: 3044 1651 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5905 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4516 136 Size: 107 122 71 631 605 2844 1525 Fragments arranged by size: 2844 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5900 5900 Size: 5905 BlpI GC'TnA_GC Cuts at: 3322 3322 Size: 5905 Bme1580I G_kGCm'C Cuts at: 1790 3966 5122 1790 Size: 2176 1156 2573 Fragments arranged by size: 2573 2176 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5905 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3636 4594 442 Size: 416 53 83 399 24 2219 958 1753 Fragments arranged by size: 2219 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5905 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2841 3093 2841 Size: 252 5653 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2117 2706 2790 2874 2958 3042 3126 4565 Size: 878 589 84 84 84 84 84 1439 Cuts at: 4565 1239 Size: 2579 Fragments arranged by size: 2579 1439 878 589 84 84 84 84 84 Bpu10I CC'TnA_GC Cuts at: 2444 2482 2444 Size: 38 5867 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4030 4895 5193 4030 Size: 865 298 4742 Fragments arranged by size: 4742 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4568 882 Size: 617 3069 2219 Fragments arranged by size: 3069 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3696 493 Size: 711 2492 2702 Fragments arranged by size: 2702 2492 711 BsaBI GATnn'nnATC Cuts at: 5899 5899 Size: 5905 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2665 3712 4094 4729 Size: 53 83 186 936 1132 1047 382 635 Cuts at: 4729 275 Size: 1451 Fragments arranged by size: 1451 1132 1047 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4338 4736 5010 5157 5568 4338 Size: 398 274 147 411 4675 Fragments arranged by size: 4675 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5680 Fragments arranged by size: 5680 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5905 BseYI C'CCAG_C Cuts at: 5108 5604 5108 Size: 496 5409 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2819 1163 Size: 573 1083 4249 Fragments arranged by size: 4249 1083 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3355 4116 5144 1540 Size: 275 186 1354 761 1028 2301 Fragments arranged by size: 2301 1354 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2990 3966 4051 5122 2990 Size: 976 85 1071 3773 Fragments arranged by size: 3773 1071 976 85 BsmI GAATG_Cn' Cuts at: 2517 2708 2792 2960 3416 3509 2517 Size: 191 84 168 456 93 4913 Fragments arranged by size: 4913 456 191 168 93 84 BsmBI CGTCTCn'nnnn_ Cuts at: 2829 2829 Size: 5905 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2181 2215 2990 3224 3966 4051 5122 Size: 399 391 34 775 234 742 85 1071 Cuts at: 5122 1391 Size: 2174 Fragments arranged by size: 2174 1071 775 742 399 391 234 85 34 BspEI T'CCGG_A Cuts at: 5568 5568 Size: 5905 BspHI T'CATG_A Cuts at: 3794 5524 3794 Size: 1730 4175 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3260 844 Size: 2416 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3792 1394 Size: 429 1969 3507 Fragments arranged by size: 3507 1969 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4400 4574 65 Size: 1183 3152 174 1396 Fragments arranged by size: 3152 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4549 4736 1700 Size: 110 2739 187 2869 Fragments arranged by size: 2869 2739 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5905 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5905 BssSI C'ACGA_G Cuts at: 3965 4977 3965 Size: 1012 4893 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5905 BstBI TT'CG_AA Cuts at: 4709 4709 Size: 5905 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5905 BstYI r'GATC_y Cuts at: 1972 2534 3235 3602 3987 4004 5456 5542 5554 Size: 562 701 367 385 17 1452 86 12 Cuts at: 5554 5900 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 701 562 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5353 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2689 487 Size: 683 56 1463 3703 Fragments arranged by size: 3703 1463 683 56 BtsI GCAGTG_nn' Cuts at: 2713 2797 3421 4215 4235 2713 Size: 84 624 794 20 4383 Fragments arranged by size: 4383 794 624 84 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5905 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5870 DraI TTT'AAA Cuts at: 3209 3209 Size: 5905 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4912 809 Size: 507 3596 1802 Fragments arranged by size: 3596 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2115 2365 3352 Size: 54 1425 50 275 18 285 250 987 Cuts at: 3352 4241 5870 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 987 889 285 275 250 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3352 1537 Size: 275 1540 4090 Fragments arranged by size: 4090 1540 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2222 2713 3835 1353 Size: 869 491 1122 3423 Fragments arranged by size: 3423 1122 869 491 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4492 4866 5012 387 Size: 1064 138 2903 374 146 1280 Fragments arranged by size: 2903 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2988 2988 Size: 5905 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2089 2117 2706 2790 2794 2874 2958 Size: 393 457 28 589 84 4 80 84 Cuts at: 2958 2962 3042 3046 3126 3968 4565 5352 5620 Size: 4 80 4 80 842 597 787 268 Cuts at: 5620 1239 Size: 1524 Fragments arranged by size: 1524 842 787 597 589 457 393 268 84 84 80 80 80 28 4 4 4 EcoO109I rG'GnC_Cy Cuts at: 2074 3141 2074 Size: 1067 4838 EcoRI G'AATT_C Cuts at: 2030 2555 3214 3592 2030 Size: 525 659 378 4343 Fragments arranged by size: 4343 659 525 378 FspI TGC'GCA Cuts at: 4411 4411 Size: 5905 HaeII r_GCGC'y Cuts at: 1678 2668 5052 1678 Size: 990 2384 2531 Fragments arranged by size: 2531 2384 990 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4546 4578 4620 4652 1373 Size: 32 135 32 2974 32 42 32 2626 Fragments arranged by size: 2974 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2418 2506 3249 3495 669 Size: 988 281 480 88 743 246 3079 Fragments arranged by size: 3079 988 743 480 281 246 88 HindIII A'AGCT_T Cuts at: 748 2479 748 Size: 1731 4174 HpaI GTT'AAC Cuts at: 2506 3495 2506 Size: 989 4916 KasI G'GCGC_C Cuts at: 2664 2664 Size: 5905 KpnI G_GTAC'C Cuts at: 3230 3230 Size: 5905 MfeI C'AATT_G Cuts at: 3504 3504 Size: 5905 MluI A'CGCG_T Cuts at: 5781 5781 Size: 5905 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2142 5019 5203 1305 Size: 837 2877 184 2007 Fragments arranged by size: 2877 2007 837 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2117 2367 10 Size: 54 1425 343 285 250 3548 Fragments arranged by size: 3548 1425 343 285 250 54 MslI CAynn'nnrTG Cuts at: 518 1166 2522 3863 4222 4381 518 Size: 648 1356 1341 359 159 2042 Fragments arranged by size: 2042 1356 1341 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2056 3321 3998 5146 5391 1606 Size: 450 1265 677 1148 245 2120 Fragments arranged by size: 2120 1265 1148 677 450 245 NarI GG'CG_CC Cuts at: 2665 2665 Size: 5905 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5353 NdeI CA'TA_TG Cuts at: 387 2146 387 Size: 1759 4146 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5905 NotI GC'GGCC_GC Cuts at: 3352 3352 Size: 5905 NspI r_CATG'y Cuts at: 1132 1229 3263 4808 1132 Size: 97 2034 1545 2229 Fragments arranged by size: 2229 2034 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5905 PciI A'CATG_T Cuts at: 4804 4804 Size: 5905 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5905 PmeI GTTT'AAAC Cuts at: 3209 3209 Size: 5905 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3974 4006 5487 5519 3974 Size: 32 1481 32 4360 Fragments arranged by size: 4360 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2074 2074 Size: 5905 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5905 PsiI TTA'TAA Cuts at: 2457 2541 3475 2457 Size: 84 934 4887 Fragments arranged by size: 4887 934 84 PstI C_TGCA'G Cuts at: 830 2331 3257 830 Size: 1501 926 3478 Fragments arranged by size: 3478 1501 926 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5905 PvuII CAG'CTG Cuts at: 2056 2056 Size: 5905 SacI G_AGCT'C Cuts at: 2990 2990 Size: 5905 SalI G'TCGA_C Cuts at: 1936 3247 1936 Size: 1311 4594 SapI GCTCTTCn'nnn_ Cuts at: 2713 2713 Size: 5905 SbfI CC_TGCA'GG Cuts at: 3257 3257 Size: 5905 ScaI AGT'ACT Cuts at: 4153 5865 4153 Size: 1712 4193 SfcI C'TryA_G Cuts at: 826 1045 2080 2327 2839 3253 4388 5069 5260 Size: 219 1035 247 512 414 1135 681 191 Cuts at: 5260 826 Size: 1471 Fragments arranged by size: 1471 1135 1035 681 512 414 247 219 191 SfoI GGC'GCC Cuts at: 2666 2666 Size: 5905 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5905 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5905 SmaI CCC'GGG Cuts at: 1872 3232 1872 Size: 1360 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4009 4910 5172 820 Size: 197 926 2066 901 262 1553 Fragments arranged by size: 2066 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3696 493 Size: 3203 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5905 SphI G_CATG'C Cuts at: 1132 3263 1132 Size: 2131 3774 SspI AAT'ATT Cuts at: 5 52 3829 5 Size: 47 3777 2081 Fragments arranged by size: 3777 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5905 StyI C'CwwG_G Cuts at: 513 1065 2244 3344 513 Size: 552 1179 1100 3074 Fragments arranged by size: 3074 1179 1100 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2613 3932 3949 4102 4287 4732 325 Size: 1255 1033 1319 17 153 185 445 1498 Fragments arranged by size: 1498 1319 1255 1033 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4151 5863 96 Size: 274 80 33 51 1443 2174 1712 138 Fragments arranged by size: 2174 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2891 2923 5865 5897 2891 Size: 32 2942 32 2899 Fragments arranged by size: 2942 2899 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5905 XbaI T'CTAG_A Cuts at: 3241 3241 Size: 5905 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5905 XmaI C'CCGG_G Cuts at: 1870 3230 1870 Size: 1360 4545 XmnI GAAnn'nnTTC Cuts at: 4034 4034 Size: 5905 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3713 276 Size: 53 83 186 936 2179 2468 Fragments arranged by size: 2468 2179 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI