(Circular) (Six-base) MAPSORT of: pFN21AB8718.seq Check: 1381 from: 1 to: 6520 pFN21AB8718 6520 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2600 2600 Size: 6520 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3270 3606 4330 278 Size: 53 83 186 936 1734 336 724 2468 Fragments arranged by size: 2468 1734 936 724 336 186 83 53 AccI GT'mk_AC Cuts at: 1937 2455 3863 1937 Size: 518 1408 4594 Fragments arranged by size: 4594 1408 518 Acc65I G'GTAC_C Cuts at: 3841 3841 Size: 6520 AclI AA'CG_TT Cuts at: 1563 4647 5020 1563 Size: 3084 373 3063 Fragments arranged by size: 3084 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3091 3245 3260 3653 4583 5967 6235 1632 Size: 1459 154 15 393 930 1384 268 1917 Fragments arranged by size: 1917 1459 1384 930 393 268 154 15 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6323 AflIII A'CryG_T Cuts at: 1203 2943 3501 3792 5419 6396 1203 Size: 1740 558 291 1627 977 1327 Fragments arranged by size: 1740 1627 1327 977 558 291 AgeI A'CCGG_T Cuts at: 5351 5351 Size: 6520 AhdI GACnn_n'nnGTC Cuts at: 5249 5249 Size: 6520 AleI CACnn'nnGTG Cuts at: 1166 2591 1166 Size: 1425 5095 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2609 2810 2900 3272 3584 1184 Size: 1425 201 90 372 312 4120 Fragments arranged by size: 4120 1425 372 312 201 90 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2930 2962 2930 Size: 32 6488 AlwNI CAG_nnn'CTG Cuts at: 4263 5835 4263 Size: 1572 4948 ApaLI G'TGCA_C Cuts at: 2677 4577 5733 2677 Size: 1900 1156 3464 Fragments arranged by size: 3464 1900 1156 ApoI r'AATT_y Cuts at: 1493 3829 4055 4207 6316 1493 Size: 2336 226 152 2109 1697 Fragments arranged by size: 2336 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5074 160 Size: 872 4042 1606 Fragments arranged by size: 4042 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6520 AvaI C'yCGr_G Cuts at: 1870 1943 2115 2237 2994 3339 3845 1870 Size: 73 172 122 757 345 506 4545 Fragments arranged by size: 4545 757 506 345 172 122 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2566 2599 2566 Size: 33 6487 BamHI G'GATC_C Cuts at: 3815 3850 4217 3815 Size: 35 367 6118 Fragments arranged by size: 6118 367 35 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2399 2691 2890 3841 5296 Size: 325 217 627 612 292 199 951 1455 Cuts at: 5296 618 Size: 1842 Fragments arranged by size: 1842 1455 951 627 612 325 292 217 199 BanII G_rGCy'C Cuts at: 1391 2043 3839 1391 Size: 652 1796 4072 Fragments arranged by size: 4072 1796 652 BbeI G_GCGC'C Cuts at: 2403 2403 Size: 6520 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5549 BbvCI CC'TCA_GC Cuts at: 3717 3717 Size: 6520 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2992 3026 3235 3269 4711 4745 1903 Size: 34 1055 34 209 34 1442 34 3678 Fragments arranged by size: 3678 1442 1055 209 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4412 5622 4412 Size: 1210 5310 BclI T'GATC_A Cuts at: 1548 1548 Size: 6520 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5131 136 Size: 107 122 71 631 605 3459 1525 Fragments arranged by size: 3459 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6515 6515 Size: 6520 BlpI GC'TnA_GC Cuts at: 2717 3937 2717 Size: 1220 5300 Bme1580I G_kGCm'C Cuts at: 1790 2681 3142 3297 3537 4581 5737 1790 Size: 891 461 155 240 1044 1156 2573 Fragments arranged by size: 2573 1156 1044 891 461 240 155 BmgBI CAC'GTC Cuts at: 1318 2227 2827 1318 Size: 909 600 5011 Fragments arranged by size: 5011 909 600 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2430 4251 5209 Size: 416 53 83 399 24 1013 1821 958 Cuts at: 5209 442 Size: 1753 Fragments arranged by size: 1821 1753 1013 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6520 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2674 2674 Size: 6520 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2450 2888 3113 3448 3644 5180 1239 Size: 1211 438 225 335 196 1536 2579 Fragments arranged by size: 2579 1536 1211 438 335 225 196 Bpu10I CC'TnA_GC Cuts at: 2742 2808 3477 3717 2742 Size: 66 669 240 5545 Fragments arranged by size: 5545 669 240 66 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2027 4645 5510 5808 2027 Size: 2618 865 298 2739 Fragments arranged by size: 2739 2618 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5183 882 Size: 617 3684 2219 Fragments arranged by size: 3684 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4311 493 Size: 711 3107 2702 Fragments arranged by size: 3107 2702 711 BsaBI GATnn'nnATC Cuts at: 2973 6514 2973 Size: 3541 2979 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2400 2960 3101 3267 Size: 53 83 186 936 867 560 141 166 Cuts at: 3267 3603 4327 4709 5344 275 Size: 336 724 382 635 1451 Fragments arranged by size: 1451 936 867 724 635 560 382 336 186 166 141 83 53 BsaWI w'CCGG_w Cuts at: 2664 2955 3744 4953 5351 5625 5772 6183 2664 Size: 291 789 1209 398 274 147 411 3001 Fragments arranged by size: 3001 1209 789 411 398 291 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2075 2105 2153 2183 2477 Size: 30 165 30 672 30 48 30 294 Cuts at: 2477 2507 2930 2960 3620 3650 1178 Size: 30 423 30 660 30 4048 Fragments arranged by size: 4048 672 660 423 294 165 48 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2099 2174 3110 3641 1186 Size: 913 75 936 531 4065 Fragments arranged by size: 4065 936 913 531 75 BseYI C'CCAG_C Cuts at: 2189 2266 2271 2570 5723 6219 2189 Size: 77 5 299 3153 496 2490 Fragments arranged by size: 3153 2490 496 299 77 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2628 2634 2652 3309 3758 1163 Size: 573 892 6 18 657 449 3925 Fragments arranged by size: 3925 892 657 573 449 18 6 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2665 2707 3970 4731 5759 1540 Size: 275 186 664 42 1263 761 1028 2301 Fragments arranged by size: 2301 1263 1028 761 664 275 186 42 BsiHKAI G_wGCw'C Cuts at: 2043 2598 2681 2799 3530 4581 4666 5737 2043 Size: 555 83 118 731 1051 85 1071 2826 Fragments arranged by size: 2826 1071 1051 731 555 118 85 83 BsmI GAATG_Cn' Cuts at: 4031 4124 4031 Size: 93 6427 BsmBI CGTCTCn'nnnn_ Cuts at: 3092 3092 Size: 6520 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2043 2598 2681 2799 3142 3297 3530 Size: 399 253 555 83 118 343 155 233 Cuts at: 3530 3537 3839 4581 4666 5737 1391 Size: 7 302 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 555 399 343 302 253 233 155 118 85 83 7 BspEI T'CCGG_A Cuts at: 2955 3744 6183 2955 Size: 789 2439 3292 Fragments arranged by size: 3292 2439 789 BspHI T'CATG_A Cuts at: 3144 4409 6139 3144 Size: 1265 1730 3525 Fragments arranged by size: 3525 1730 1265 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2600 3875 844 Size: 1756 1275 3489 Fragments arranged by size: 3489 1756 1275 BsrBI CCG'CTC Cuts at: 1394 1823 4407 1394 Size: 429 2584 3507 Fragments arranged by size: 3507 2584 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2137 5015 5189 65 Size: 1183 889 2878 174 1396 Fragments arranged by size: 2878 1396 1183 889 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2688 3023 5164 5351 1700 Size: 110 878 335 2141 187 2869 Fragments arranged by size: 2869 2141 878 335 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6520 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6520 BssSI C'ACGA_G Cuts at: 4580 5592 4580 Size: 1012 5508 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6520 BstBI TT'CG_AA Cuts at: 5324 5324 Size: 6520 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6520 BstXI CCAn_nnnn'nTGG Cuts at: 2035 2132 2545 2980 2035 Size: 97 413 435 5575 Fragments arranged by size: 5575 435 413 97 BstYI r'GATC_y Cuts at: 1972 3619 3815 3850 4217 4602 4619 6071 6157 Size: 1647 196 35 367 385 17 1452 86 Cuts at: 6157 6169 6515 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1647 1452 385 367 346 196 86 35 17 12 Bsu36I CC'TnA_GG Cuts at: 2196 2756 3311 3730 2196 Size: 560 555 419 4986 Fragments arranged by size: 4986 560 555 419 BtgI C'CryG_G Cuts at: 513 1065 2004 2371 3675 513 Size: 552 939 367 1304 3358 Fragments arranged by size: 3358 1304 939 552 367 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3417 487 Size: 683 56 2191 3590 Fragments arranged by size: 3590 2191 683 56 BtsI GCAGTG_nn' Cuts at: 2639 3297 4036 4830 4850 2639 Size: 658 739 794 20 4309 Fragments arranged by size: 4309 794 739 658 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6520 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6485 DraI TTT'AAA Cuts at: 3824 3824 Size: 6520 DraIII CAC_nnn'GTG Cuts at: 3076 3076 Size: 6520 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2669 3193 5527 809 Size: 507 1353 524 2334 1802 Fragments arranged by size: 2334 1802 1353 524 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2094 2245 2547 Size: 54 1425 50 275 18 264 151 302 Cuts at: 2547 3045 3678 3967 4856 6485 8 Size: 498 633 289 889 1629 43 Fragments arranged by size: 1629 1425 889 633 498 302 289 275 264 151 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3967 1537 Size: 275 2155 4090 Fragments arranged by size: 4090 2155 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3236 4450 1353 Size: 1883 1214 3423 Fragments arranged by size: 3423 1883 1214 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3533 5107 5481 5627 387 Size: 1064 138 1944 1574 374 146 1280 Fragments arranged by size: 1944 1574 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2041 2041 Size: 6520 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2450 2888 3091 3113 3245 3260 3448 Size: 393 818 438 203 22 132 15 188 Cuts at: 3448 3644 3653 4583 5180 5967 6235 1239 Size: 196 9 930 597 787 268 1524 Fragments arranged by size: 1524 930 818 787 597 438 393 268 203 196 188 132 22 15 9 EcoNI CCTnn'n_nnAGG Cuts at: 3109 3109 Size: 6520 EcoO109I rG'GnC_Cy Cuts at: 2233 2425 2515 2233 Size: 192 90 6238 Fragments arranged by size: 6238 192 90 EcoRI G'AATT_C Cuts at: 3829 4207 3829 Size: 378 6142 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2875 2875 Size: 6520 FspI TGC'GCA Cuts at: 5026 5026 Size: 6520 HaeII r_GCGC'y Cuts at: 1678 2167 2403 5667 1678 Size: 489 236 3264 2531 Fragments arranged by size: 3264 2531 489 236 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2075 2107 2153 2185 3333 Size: 32 135 32 503 32 46 32 1148 Cuts at: 3333 3365 5161 5193 5235 5267 1373 Size: 32 1796 32 42 32 2626 Fragments arranged by size: 2626 1796 1148 503 135 46 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3358 3864 4110 669 Size: 988 281 1420 506 246 3079 Fragments arranged by size: 3079 1420 988 506 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6520 HpaI GTT'AAC Cuts at: 4110 4110 Size: 6520 KasI G'GCGC_C Cuts at: 2399 2399 Size: 6520 KpnI G_GTAC'C Cuts at: 3845 3845 Size: 6520 MfeI C'AATT_G Cuts at: 4119 4119 Size: 6520 MluI A'CGCG_T Cuts at: 6396 6396 Size: 6520 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2685 5634 5818 1305 Size: 1380 2949 184 2007 Fragments arranged by size: 2949 2007 1380 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2247 2549 3680 10 Size: 54 1425 343 415 302 1131 2850 Fragments arranged by size: 2850 1425 1131 415 343 302 54 MslI CAynn'nnrTG Cuts at: 518 1166 2591 2792 3149 4478 4837 4996 518 Size: 648 1425 201 357 1329 359 159 2042 Fragments arranged by size: 2042 1425 1329 648 359 357 201 159 MspA1I CmG'CkG Cuts at: 1606 2524 2618 2749 3398 3936 4613 5761 6006 Size: 918 94 131 649 538 677 1148 245 Cuts at: 6006 1606 Size: 2120 Fragments arranged by size: 2120 1148 918 677 649 538 245 131 94 NaeI GCC'GGC Cuts at: 3025 3025 Size: 6520 NarI GG'CG_CC Cuts at: 2400 2400 Size: 6520 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5029 Fragments arranged by size: 5029 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6520 NgoMIV G'CCGG_C Cuts at: 3023 3023 Size: 6520 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6520 NotI GC'GGCC_GC Cuts at: 3967 3967 Size: 6520 NspI r_CATG'y Cuts at: 1132 1229 3184 3505 3796 3878 5423 1132 Size: 97 1955 321 291 82 1545 2229 Fragments arranged by size: 2229 1955 1545 321 291 97 82 PasI CC'CwG_GG Cuts at: 1348 2317 1348 Size: 969 5551 PciI A'CATG_T Cuts at: 3501 3792 5419 3501 Size: 291 1627 4602 Fragments arranged by size: 4602 1627 291 PflMI CCAn_nnn'nTGG Cuts at: 1108 2317 2570 1108 Size: 1209 253 5058 Fragments arranged by size: 5058 1209 253 PmeI GTTT'AAAC Cuts at: 3824 3824 Size: 6520 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2930 2962 4589 4621 6102 6134 2930 Size: 32 1627 32 1481 32 3316 Fragments arranged by size: 3316 1627 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2233 2425 2233 Size: 192 6328 PshAI GACnn'nnGTC Cuts at: 1613 3377 1613 Size: 1764 4756 PsiI TTA'TAA Cuts at: 4090 4090 Size: 6520 PstI C_TGCA'G Cuts at: 830 2529 3872 830 Size: 1699 1343 3478 Fragments arranged by size: 3478 1699 1343 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6520 PvuII CAG'CTG Cuts at: 2524 2618 2749 3398 2524 Size: 94 131 649 5646 Fragments arranged by size: 5646 649 131 94 SacI G_AGCT'C Cuts at: 2043 2043 Size: 6520 SalI G'TCGA_C Cuts at: 1936 3862 1936 Size: 1926 4594 SapI GCTCTTCn'nnn_ Cuts at: 3236 3236 Size: 6520 SbfI CC_TGCA'GG Cuts at: 3872 3872 Size: 6520 ScaI AGT'ACT Cuts at: 4768 6480 4768 Size: 1712 4808 SfcI C'TryA_G Cuts at: 826 1045 2519 2525 3868 5003 5684 5875 826 Size: 219 1474 6 1343 1135 681 191 1471 Fragments arranged by size: 1474 1471 1343 1135 681 219 191 6 SfoI GGC'GCC Cuts at: 2401 2401 Size: 6520 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6520 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6520 SmaI CCC'GGG Cuts at: 1872 2117 3847 1872 Size: 245 1730 4545 Fragments arranged by size: 4545 1730 245 SmlI C'TyrA_G Cuts at: 820 1017 1943 2042 2237 4624 5525 5787 820 Size: 197 926 99 195 2387 901 262 1553 Fragments arranged by size: 2387 1553 926 901 262 197 195 99 SnaBI TAC'GTA Cuts at: 493 4311 493 Size: 3818 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6520 SphI G_CATG'C Cuts at: 1132 3878 1132 Size: 2746 3774 SspI AAT'ATT Cuts at: 5 52 4444 5 Size: 47 4392 2081 Fragments arranged by size: 4392 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6520 StyI C'CwwG_G Cuts at: 513 1065 2004 2538 2763 3708 3959 513 Size: 552 939 534 225 945 251 3074 Fragments arranged by size: 3074 945 939 552 534 251 225 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2594 4547 4564 4717 4902 5347 325 Size: 1255 1014 1953 17 153 185 445 1498 Fragments arranged by size: 1953 1498 1255 1014 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2871 4766 6478 Size: 274 80 33 51 1443 894 1895 1712 Cuts at: 6478 96 Size: 138 Fragments arranged by size: 1895 1712 1443 894 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6480 6512 6480 Size: 32 6488 Tth111I GACn'n_nGTC Cuts at: 1316 3602 1316 Size: 2286 4234 XbaI T'CTAG_A Cuts at: 3856 3856 Size: 6520 XhoI C'TCGA_G Cuts at: 1943 2237 1943 Size: 294 6226 XmaI C'CCGG_G Cuts at: 1870 2115 3845 1870 Size: 245 1730 4545 Fragments arranged by size: 4545 1730 245 XmnI GAAnn'nnTTC Cuts at: 2945 4649 2945 Size: 1704 4816 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3268 3604 4328 276 Size: 53 83 186 936 1734 336 724 2468 Fragments arranged by size: 2468 1734 936 724 336 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BfrBI BsiWI BstZ17I EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI