(Circular) (Six-base) MAPSORT of: pFN21AB8677.seq Check: 5305 from: 1 to: 5809 pFN21AB8677 5809 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3619 278 Size: 53 83 186 936 2083 2468 Fragments arranged by size: 2468 2083 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2132 3152 1937 Size: 195 1020 4594 Fragments arranged by size: 4594 1020 195 Acc65I G'GTAC_C Cuts at: 3130 3130 Size: 5809 AclI AA'CG_TT Cuts at: 1563 3936 4309 1563 Size: 2373 373 3063 Fragments arranged by size: 3063 2373 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3872 5256 5524 1632 Size: 2240 1384 268 1917 Fragments arranged by size: 2240 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5612 AflIII A'CryG_T Cuts at: 1203 2728 2781 2991 4708 5685 1203 Size: 1525 53 210 1717 977 1327 Fragments arranged by size: 1717 1525 1327 977 210 53 AgeI A'CCGG_T Cuts at: 4640 4640 Size: 5809 AhdI GACnn_n'nnGTC Cuts at: 4538 4538 Size: 5809 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5809 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5809 AlwNI CAG_nnn'CTG Cuts at: 2049 2413 2465 3552 5124 2049 Size: 364 52 1087 1572 2734 Fragments arranged by size: 2734 1572 1087 364 52 ApaI G_GGCC'C Cuts at: 2032 2077 2503 2032 Size: 45 426 5338 Fragments arranged by size: 5338 426 45 ApaLI G'TGCA_C Cuts at: 3866 5022 3866 Size: 1156 4653 ApoI r'AATT_y Cuts at: 1493 2773 3118 3344 3496 5605 1493 Size: 1280 345 226 152 2109 1697 Fragments arranged by size: 2109 1697 1280 345 226 152 AseI AT'TA_AT Cuts at: 160 1032 4363 160 Size: 872 3331 1606 Fragments arranged by size: 3331 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5809 AvaI C'yCGr_G Cuts at: 1870 1943 2643 3134 1870 Size: 73 700 491 4545 Fragments arranged by size: 4545 700 491 73 BamHI G'GATC_C Cuts at: 3139 3506 3139 Size: 367 5442 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2151 2212 2876 3130 4585 Size: 325 217 627 364 61 664 254 1455 Cuts at: 4585 618 Size: 1842 Fragments arranged by size: 1842 1455 664 627 364 325 254 217 61 BanII G_rGCy'C Cuts at: 1391 2032 2077 2503 2694 3128 1391 Size: 641 45 426 191 434 4072 Fragments arranged by size: 4072 641 434 426 191 45 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2547 3089 928 Size: 971 648 542 3648 Fragments arranged by size: 3648 971 648 542 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4000 4034 1903 Size: 34 2063 34 3678 Fragments arranged by size: 3678 2063 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2813 3701 4911 2813 Size: 888 1210 3711 Fragments arranged by size: 3711 1210 888 BclI T'GATC_A Cuts at: 1548 2566 1548 Size: 1018 4791 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2027 4420 136 Size: 107 122 71 631 605 355 2393 1525 Fragments arranged by size: 2393 1525 631 605 355 122 107 71 BglII A'GATC_T Cuts at: 2450 5804 2450 Size: 3354 2455 BlpI GC'TnA_GC Cuts at: 3226 3226 Size: 5809 Bme1580I G_kGCm'C Cuts at: 1790 2032 2077 2503 2991 3002 3870 5026 1790 Size: 242 45 426 488 11 868 1156 2573 Fragments arranged by size: 2573 1156 868 488 426 242 45 11 BmgBI CAC'GTC Cuts at: 1318 2965 1318 Size: 1647 4162 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2079 3115 3540 Size: 416 53 83 399 24 662 1036 425 Cuts at: 3540 4498 442 Size: 958 1753 Fragments arranged by size: 1753 1036 958 662 425 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5809 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2133 2322 2708 2875 4469 1239 Size: 894 189 386 167 1594 2579 Fragments arranged by size: 2579 1594 894 386 189 167 Bpu10I CC'TnA_GC Cuts at: 2654 2654 Size: 5809 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3934 4799 5097 3934 Size: 865 298 4646 Fragments arranged by size: 4646 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2231 4472 882 Size: 617 732 2241 2219 Fragments arranged by size: 2241 2219 732 617 BsaAI yAC'GTr Cuts at: 493 1204 2729 2782 3600 493 Size: 711 1525 53 818 2702 Fragments arranged by size: 2702 1525 818 711 53 BsaBI GATnn'nnATC Cuts at: 2703 5803 2703 Size: 3100 2709 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3616 3998 4633 275 Size: 53 83 186 936 2083 382 635 1451 Fragments arranged by size: 2083 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4242 4640 4914 5061 5472 4242 Size: 398 274 147 411 4579 Fragments arranged by size: 4579 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5584 Fragments arranged by size: 5584 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5809 BseYI C'CCAG_C Cuts at: 5012 5508 5012 Size: 496 5313 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5236 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2063 2106 3259 4020 5048 1540 Size: 275 186 62 43 1153 761 1028 2301 Fragments arranged by size: 2301 1153 1028 761 275 186 62 43 BsiHKAI G_wGCw'C Cuts at: 2694 3870 3955 5026 2694 Size: 1176 85 1071 3477 Fragments arranged by size: 3477 1176 1071 85 BsmI GAATG_Cn' Cuts at: 3320 3413 3320 Size: 93 5716 BsmBI CGTCTCn'nnnn_ Cuts at: 2642 2971 2642 Size: 329 5480 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2032 2077 2503 2694 2991 3002 3128 Size: 399 242 45 426 191 297 11 126 Cuts at: 3128 3870 3955 5026 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 426 399 297 242 191 126 85 45 11 BspEI T'CCGG_A Cuts at: 5472 5472 Size: 5809 BspHI T'CATG_A Cuts at: 3698 5428 3698 Size: 1730 4079 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3164 844 Size: 2320 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2092 2848 3696 1394 Size: 429 269 756 848 3507 Fragments arranged by size: 3507 848 756 429 269 BsrDI GCAATG_nn' Cuts at: 65 1248 4304 4478 65 Size: 1183 3056 174 1396 Fragments arranged by size: 3056 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4453 4640 1700 Size: 110 2643 187 2869 Fragments arranged by size: 2869 2643 187 110 BsrGI T'GTAC_A Cuts at: 96 2015 96 Size: 1919 3890 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5809 BssSI C'ACGA_G Cuts at: 2424 3869 4881 2424 Size: 1445 1012 3352 Fragments arranged by size: 3352 1445 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5809 BstBI TT'CG_AA Cuts at: 4613 4613 Size: 5809 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5809 BstYI r'GATC_y Cuts at: 1972 2450 3139 3506 3891 3908 5360 5446 5458 Size: 478 689 367 385 17 1452 86 12 Cuts at: 5458 5804 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 689 478 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3094 3094 Size: 5809 BtgI C'CryG_G Cuts at: 513 1065 2004 2037 2365 513 Size: 552 939 33 328 3957 Fragments arranged by size: 3957 939 552 328 33 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5070 Fragments arranged by size: 5070 683 56 BtsI GCAGTG_nn' Cuts at: 3325 4119 4139 3325 Size: 794 20 4995 Fragments arranged by size: 4995 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5809 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5774 DraI TTT'AAA Cuts at: 3113 3113 Size: 5809 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4816 809 Size: 507 3500 1802 Fragments arranged by size: 3500 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2034 2081 2103 Size: 54 1425 50 275 18 204 47 22 Cuts at: 2103 2362 3256 4145 5774 8 Size: 259 894 889 1629 43 Fragments arranged by size: 1629 1425 894 889 275 259 204 54 50 47 43 22 18 EagI C'GGCC_G Cuts at: 1537 1812 2103 3256 1537 Size: 275 291 1153 4090 Fragments arranged by size: 4090 1153 291 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3739 1353 Size: 2386 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4396 4770 4916 387 Size: 1064 138 2807 374 146 1280 Fragments arranged by size: 2807 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2692 2692 Size: 5809 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2133 2322 2708 2875 3872 4469 5256 Size: 393 501 189 386 167 997 597 787 Cuts at: 5256 5524 1239 Size: 268 1524 Fragments arranged by size: 1524 997 787 597 501 393 386 268 189 167 EcoO109I rG'GnC_Cy Cuts at: 2029 2073 2500 2658 2029 Size: 44 427 158 5180 Fragments arranged by size: 5180 427 158 44 EcoRI G'AATT_C Cuts at: 2773 3118 3496 2773 Size: 345 378 5086 Fragments arranged by size: 5086 378 345 FspI TGC'GCA Cuts at: 4315 4315 Size: 5809 HaeII r_GCGC'y Cuts at: 1678 2797 4956 1678 Size: 1119 2159 2531 Fragments arranged by size: 2531 2159 1119 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2268 2300 2407 2439 2851 Size: 32 135 32 696 32 107 32 412 Cuts at: 2851 2883 4450 4482 4524 4556 1373 Size: 32 1567 32 42 32 2626 Fragments arranged by size: 2626 1567 696 412 135 107 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2133 3153 3399 669 Size: 988 281 195 1020 246 3079 Fragments arranged by size: 3079 1020 988 281 246 195 HindIII A'AGCT_T Cuts at: 748 748 Size: 5809 HpaI GTT'AAC Cuts at: 3399 3399 Size: 5809 KpnI G_GTAC'C Cuts at: 3134 3134 Size: 5809 MfeI C'AATT_G Cuts at: 3408 3408 Size: 5809 MluI A'CGCG_T Cuts at: 5685 5685 Size: 5809 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2169 2605 4923 5107 1305 Size: 864 436 2318 184 2007 Fragments arranged by size: 2318 2007 864 436 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2083 2364 10 Size: 54 1425 343 251 281 3455 Fragments arranged by size: 3455 1425 343 281 251 54 MslI CAynn'nnrTG Cuts at: 518 1166 2756 3036 3767 4126 4285 518 Size: 648 1590 280 731 359 159 2042 Fragments arranged by size: 2042 1590 731 648 359 280 159 MspA1I CmG'CkG Cuts at: 1606 2049 3225 3902 5050 5295 1606 Size: 443 1176 677 1148 245 2120 Fragments arranged by size: 2120 1176 1148 677 443 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2365 513 Size: 552 939 361 3957 Fragments arranged by size: 3957 939 552 361 NdeI CA'TA_TG Cuts at: 387 387 Size: 5809 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5809 NotI GC'GGCC_GC Cuts at: 3256 3256 Size: 5809 NspI r_CATG'y Cuts at: 1132 1229 2995 3167 4712 1132 Size: 97 1766 172 1545 2229 Fragments arranged by size: 2229 1766 1545 172 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5809 PciI A'CATG_T Cuts at: 2991 4708 2991 Size: 1717 4092 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5809 PfoI T'CCnGG_A Cuts at: 2841 2841 Size: 5809 PmeI GTTT'AAAC Cuts at: 3113 3113 Size: 5809 PmlI CAC'GTG Cuts at: 2782 2782 Size: 5809 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3878 3910 5391 5423 3878 Size: 32 1481 32 4264 Fragments arranged by size: 4264 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5809 PsiI TTA'TAA Cuts at: 3379 3379 Size: 5809 PspOMI G'GGCC_C Cuts at: 2028 2073 2499 2028 Size: 45 426 5338 Fragments arranged by size: 5338 426 45 PstI C_TGCA'G Cuts at: 830 2335 2976 3161 830 Size: 1505 641 185 3478 Fragments arranged by size: 3478 1505 641 185 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5809 SacI G_AGCT'C Cuts at: 2694 2694 Size: 5809 SalI G'TCGA_C Cuts at: 1936 2131 3151 1936 Size: 195 1020 4594 Fragments arranged by size: 4594 1020 195 SbfI CC_TGCA'GG Cuts at: 3161 3161 Size: 5809 ScaI AGT'ACT Cuts at: 4057 5769 4057 Size: 1712 4097 SfcI C'TryA_G Cuts at: 826 1045 2331 2972 3157 4292 4973 5164 826 Size: 219 1286 641 185 1135 681 191 1471 Fragments arranged by size: 1471 1286 1135 681 641 219 191 185 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5809 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5809 SmaI CCC'GGG Cuts at: 1872 3136 1872 Size: 1264 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3913 4814 5076 820 Size: 197 926 1970 901 262 1553 Fragments arranged by size: 1970 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3600 493 Size: 3107 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5809 SphI G_CATG'C Cuts at: 1132 3167 1132 Size: 2035 3774 SspI AAT'ATT Cuts at: 5 52 3733 5 Size: 47 3681 2081 Fragments arranged by size: 3681 2081 47 StuI AGG'CCT Cuts at: 1517 3099 1517 Size: 1582 4227 StyI C'CwwG_G Cuts at: 513 1065 2004 2084 2365 2373 2880 2931 3248 Size: 552 939 80 281 8 507 51 317 Cuts at: 3248 513 Size: 3074 Fragments arranged by size: 3074 939 552 507 317 281 80 51 8 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3836 3853 4006 4191 4636 325 Size: 1255 2256 17 153 185 445 1498 Fragments arranged by size: 2256 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2015 2784 4055 Size: 274 80 33 51 1443 38 769 1271 Cuts at: 4055 5767 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1271 769 274 138 80 51 38 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2144 2176 5769 5801 2144 Size: 32 3593 32 2152 Fragments arranged by size: 3593 2152 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2528 1316 Size: 1212 4597 XbaI T'CTAG_A Cuts at: 3145 3145 Size: 5809 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2518 2518 Size: 5809 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5809 XmaI C'CCGG_G Cuts at: 1870 3134 1870 Size: 1264 4545 XmnI GAAnn'nnTTC Cuts at: 2601 3938 2601 Size: 1337 4472 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3617 276 Size: 53 83 186 936 2083 2468 Fragments arranged by size: 2468 2083 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI