Restriction map for
pFN21AB8677
(Linear) (Six-base) MAPSORT of: KIBB8677.seq Check: 7930 from: 1 to: 1104
KIBB8677.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AccI GT'mk_AC
Cuts at: 0 127 1104
Size: 127 977
AflIII A'CryG_T
Cuts at: 0 723 776 986 1104
Size: 723 53 210 118
Fragments arranged by size:
723 210 118 53
AlwNI CAG_nnn'CTG
Cuts at: 0 44 408 460 1104
Size: 44 364 52 644
Fragments arranged by size:
644 364 52 44
ApaI G_GGCC'C
Cuts at: 0 27 72 498 1104
Size: 27 45 426 606
Fragments arranged by size:
606 426 45 27
ApoI r'AATT_y
Cuts at: 0 768 1104
Size: 768 336
AvaI C'yCGr_G
Cuts at: 0 638 1104
Size: 638 466
BanI G'GyrC_C
Cuts at: 0 146 207 871 1104
Size: 146 61 664 233
Fragments arranged by size:
664 233 146 61
BanII G_rGCy'C
Cuts at: 0 27 72 498 689 1104
Size: 27 45 426 191 415
Fragments arranged by size:
426 415 191 45 27
BbsI GAAGACnn'nnnn_
Cuts at: 0 542 1084 1104
Size: 542 542 20
Fragments arranged by size:
542 542 20
BciVI GTATCCnnnnn_n'
Cuts at: 0 808 1104
Size: 808 296
BclI T'GATC_A
Cuts at: 0 561 1104
Size: 561 543
BglI GCCn_nnn'nGGC
Cuts at: 0 22 1104
Size: 22 1082
BglII A'GATC_T
Cuts at: 0 445 1104
Size: 445 659
Bme1580I G_kGCm'C
Cuts at: 0 27 72 498 986 997 1104
Size: 27 45 426 488 11 107
Fragments arranged by size:
488 426 107 45 27 11
BmgBI CAC'GTC
Cuts at: 0 960 1104
Size: 960 144
BmrI ACTGGGnnnn_n'
Cuts at: 0 74 1104
Size: 74 1030
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 128 317 703 870 1104
Size: 128 189 386 167 234
Fragments arranged by size:
386 234 189 167 128
Bpu10I CC'TnA_GC
Cuts at: 0 649 1104
Size: 649 455
BsaI GGTCTCn'nnnn_
Cuts at: 0 226 1104
Size: 226 878
BsaAI yAC'GTr
Cuts at: 0 724 777 1104
Size: 724 53 327
Fragments arranged by size:
724 327 53
BsaBI GATnn'nnATC
Cuts at: 0 698 1104
Size: 698 406
BsiEI CG_ry'CG
Cuts at: 0 58 101 1104
Size: 58 43 1003
Fragments arranged by size:
1003 58 43
BsiHKAI G_wGCw'C
Cuts at: 0 689 1104
Size: 689 415
BsmBI CGTCTCn'nnnn_
Cuts at: 0 637 966 1104
Size: 637 329 138
Fragments arranged by size:
637 329 138
Bsp1286I G_dGCh'C
Cuts at: 0 27 72 498 689 986 997 1104
Size: 27 45 426 191 297 11 107
Fragments arranged by size:
426 297 191 107 45 27 11
BsrBI CCG'CTC
Cuts at: 0 87 843 1104
Size: 87 756 261
Fragments arranged by size:
756 261 87
BsrGI T'GTAC_A
Cuts at: 0 10 1104
Size: 10 1094
BssSI C'ACGA_G
Cuts at: 0 419 1104
Size: 419 685
BstYI r'GATC_y
Cuts at: 0 445 1104
Size: 445 659
Bsu36I CC'TnA_GG
Cuts at: 0 1089 1104
Size: 1089 15
BtgI C'CryG_G
Cuts at: 0 32 360 1104
Size: 32 328 744
Fragments arranged by size:
744 328 32
EaeI y'GGCC_r
Cuts at: 0 29 76 98 357 1104
Size: 29 47 22 259 747
Fragments arranged by size:
747 259 47 29 22
EagI C'GGCC_G
Cuts at: 0 98 1104
Size: 98 1006
EcoICRI GAG'CTC
Cuts at: 0 687 1104
Size: 687 417
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 128 317 703 870 1104
Size: 128 189 386 167 234
Fragments arranged by size:
386 234 189 167 128
EcoO109I rG'GnC_Cy
Cuts at: 0 24 68 495 653 1104
Size: 24 44 427 158 451
Fragments arranged by size:
451 427 158 44 24
EcoRI G'AATT_C
Cuts at: 0 768 1104
Size: 768 336
HaeII r_GCGC'y
Cuts at: 0 792 1104
Size: 792 312
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 263 295 402 434 846 878 1104
Size: 263 32 107 32 412 32 226
Fragments arranged by size:
412 263 226 107 32 32 32
HincII GTy'rAC
Cuts at: 0 128 1104
Size: 128 976
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 164 600 1104
Size: 164 436 504
Fragments arranged by size:
504 436 164
MscI TGG'CCA
Cuts at: 0 78 359 1104
Size: 78 281 745
Fragments arranged by size:
745 281 78
MslI CAynn'nnrTG
Cuts at: 0 751 1031 1104
Size: 751 280 73
Fragments arranged by size:
751 280 73
MspA1I CmG'CkG
Cuts at: 0 44 1104
Size: 44 1060
NcoI C'CATG_G
Cuts at: 0 360 1104
Size: 360 744
NspI r_CATG'y
Cuts at: 0 990 1104
Size: 990 114
PciI A'CATG_T
Cuts at: 0 986 1104
Size: 986 118
PfoI T'CCnGG_A
Cuts at: 0 836 1104
Size: 836 268
PmlI CAC'GTG
Cuts at: 0 777 1104
Size: 777 327
PspOMI G'GGCC_C
Cuts at: 0 23 68 494 1104
Size: 23 45 426 610
Fragments arranged by size:
610 426 45 23
PstI C_TGCA'G
Cuts at: 0 330 971 1104
Size: 330 641 133
Fragments arranged by size:
641 330 133
SacI G_AGCT'C
Cuts at: 0 689 1104
Size: 689 415
SalI G'TCGA_C
Cuts at: 0 126 1104
Size: 126 978
SfcI C'TryA_G
Cuts at: 0 326 967 1104
Size: 326 641 137
Fragments arranged by size:
641 326 137
StuI AGG'CCT
Cuts at: 0 1094 1104
Size: 1094 10
StyI C'CwwG_G
Cuts at: 0 79 360 368 875 926 1104
Size: 79 281 8 507 51 178
Fragments arranged by size:
507 281 178 79 51 8
TatI w'GTAC_w
Cuts at: 0 10 779 1104
Size: 10 769 325
Fragments arranged by size:
769 325 10
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 139 171 1104
Size: 139 32 933
Fragments arranged by size:
933 139 32
Tth111I GACn'n_nGTC
Cuts at: 0 523 1104
Size: 523 581
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 513 1104
Size: 513 591
XmnI GAAnn'nnTTC
Cuts at: 0 596 1104
Size: 596 508
Enzymes that do cut:
AccI AflIII AlwNI ApaI ApoI AvaI BanI BanII
BbsI BciVI BclI BglI BglII Bme1580I BmgBI BmrI
BpmI Bpu10I BsaI BsaAI BsaBI BsiEI BsiHKAI BsmBI
Bsp1286I BsrBI BsrGI BssSI BstYI Bsu36I BtgI EaeI
EagI EcoICRI Eco57MI EcoO109I EcoRI HaeII Hin4I HincII
MmeI MscI MslI MspA1I NcoI NspI PciI PfoI
PmlI PspOMI PstI SacI SalI SfcI StuI StyI
TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BcgI BfrBI BlpI
BmtI BplI BpuEI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiWI BsmI BspEI BspHI BspMI BsrDI BsrFI
BssHII BstAPI BstBI BstEII BstXI BstZ17I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EarI EciI EcoNI
EcoRV FalI FseI FspI FspAI HindIII HpaI KasI
KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PflMI PmeI PpiI
PpuMI PshAI PsiI PspXI PsrI PvuI PvuII RsrII
SacII SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI SwaI TaqII XbaI XhoI XmaI ZraI