Restriction map for
pFN21AB8672
(Linear) (Six-base) MAPSORT of: KIBB8672.seq Check: 4591 from: 1 to: 1713
KIBB8672.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AccI GT'mk_AC
Cuts at: 0 188 1713
Size: 188 1525
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1571 1713
Size: 1571 142
AflIII A'CryG_T
Cuts at: 0 1292 1713
Size: 1292 421
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 625 657 1713
Size: 625 32 1056
Fragments arranged by size:
1056 625 32
AlwNI CAG_nnn'CTG
Cuts at: 0 211 1008 1437 1713
Size: 211 797 429 276
Fragments arranged by size:
797 429 276 211
ApoI r'AATT_y
Cuts at: 0 73 429 747 1713
Size: 73 356 318 966
Fragments arranged by size:
966 356 318 73
BamHI G'GATC_C
Cuts at: 0 43 1713
Size: 43 1670
BanII G_rGCy'C
Cuts at: 0 158 872 1713
Size: 158 714 841
Fragments arranged by size:
841 714 158
BbsI GAAGACnn'nnnn_
Cuts at: 0 500 1713
Size: 500 1213
BciVI GTATCCnnnnn_n'
Cuts at: 0 651 1713
Size: 651 1062
BclI T'GATC_A
Cuts at: 0 404 1713
Size: 404 1309
BglI GCCn_nnn'nGGC
Cuts at: 0 568 1713
Size: 568 1145
BlpI GC'TnA_GC
Cuts at: 0 866 1713
Size: 866 847
Bme1580I G_kGCm'C
Cuts at: 0 1352 1713
Size: 1352 361
BmrI ACTGGGnnnn_n'
Cuts at: 0 252 595 1713
Size: 252 343 1118
Fragments arranged by size:
1118 343 252
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 799 1713
Size: 799 914
BsaI GGTCTCn'nnnn_
Cuts at: 0 903 1713
Size: 903 810
BsaHI Gr'CG_yC
Cuts at: 0 1382 1713
Size: 1382 331
BseYI C'CCAG_C
Cuts at: 0 690 1713
Size: 690 1023
BsiHKAI G_wGCw'C
Cuts at: 0 158 1713
Size: 158 1555
BsmI GAATG_Cn'
Cuts at: 0 261 525 1713
Size: 261 264 1188
Fragments arranged by size:
1188 264 261
Bsp1286I G_dGCh'C
Cuts at: 0 158 872 1352 1713
Size: 158 714 480 361
Fragments arranged by size:
714 480 361 158
BsrDI GCAATG_nn'
Cuts at: 0 1385 1601 1713
Size: 1385 216 112
Fragments arranged by size:
1385 216 112
BsrGI T'GTAC_A
Cuts at: 0 1482 1713
Size: 1482 231
BstAPI GCAn_nnn'nTGC
Cuts at: 0 424 1713
Size: 424 1289
BstXI CCAn_nnnn'nTGG
Cuts at: 0 651 1713
Size: 651 1062
BstYI r'GATC_y
Cuts at: 0 43 1713
Size: 43 1670
BtgI C'CryG_G
Cuts at: 0 372 566 1713
Size: 372 194 1147
Fragments arranged by size:
1147 372 194
EaeI y'GGCC_r
Cuts at: 0 50 434 576 1713
Size: 50 384 142 1137
Fragments arranged by size:
1137 384 142 50
EarI CTCTTCn'nnn_
Cuts at: 0 716 1713
Size: 716 997
EcoICRI GAG'CTC
Cuts at: 0 156 1713
Size: 156 1557
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 799 1571 1713
Size: 799 772 142
Fragments arranged by size:
799 772 142
EcoRV GAT'ATC
Cuts at: 0 1221 1713
Size: 1221 492
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 730 1713
Size: 730 983
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 218 250 368 400 1210 1242 1713
Size: 218 32 118 32 810 32 471
Fragments arranged by size:
810 471 218 118 32 32 32
HincII GTy'rAC
Cuts at: 0 548 1713
Size: 548 1165
HindIII A'AGCT_T
Cuts at: 0 1553 1713
Size: 1553 160
HpaI GTT'AAC
Cuts at: 0 548 1713
Size: 548 1165
MscI TGG'CCA
Cuts at: 0 436 578 1713
Size: 436 142 1135
Fragments arranged by size:
1135 436 142
MslI CAynn'nnrTG
Cuts at: 0 328 350 649 1713
Size: 328 22 299 1064
Fragments arranged by size:
1064 328 299 22
MspA1I CmG'CkG
Cuts at: 0 744 1713
Size: 744 969
NcoI C'CATG_G
Cuts at: 0 372 566 1713
Size: 372 194 1147
Fragments arranged by size:
1147 372 194
NspI r_CATG'y
Cuts at: 0 1296 1713
Size: 1296 417
PciI A'CATG_T
Cuts at: 0 1292 1713
Size: 1292 421
PfoI T'CCnGG_A
Cuts at: 0 1325 1713
Size: 1325 388
PshAI GACnn'nnGTC
Cuts at: 0 184 1713
Size: 184 1529
PsiI TTA'TAA
Cuts at: 0 1474 1713
Size: 1474 239
PvuII CAG'CTG
Cuts at: 0 744 1713
Size: 744 969
SacI G_AGCT'C
Cuts at: 0 158 1713
Size: 158 1555
ScaI AGT'ACT
Cuts at: 0 441 1713
Size: 441 1272
SfcI C'TryA_G
Cuts at: 0 1070 1713
Size: 1070 643
SspI AAT'ATT
Cuts at: 0 423 1320 1713
Size: 423 897 393
Fragments arranged by size:
897 423 393
StyI C'CwwG_G
Cuts at: 0 47 87 372 566 1713
Size: 47 40 285 194 1147
Fragments arranged by size:
1147 285 194 47 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 234 787 1390 1713
Size: 234 553 603 323
Fragments arranged by size:
603 553 323 234
TatI w'GTAC_w
Cuts at: 0 439 1482 1713
Size: 439 1043 231
Fragments arranged by size:
1043 439 231
Enzymes that do cut:
AccI AcuI AflIII AloI AlwNI ApoI BamHI BanII
BbsI BciVI BclI BglI BlpI Bme1580I BmrI BpmI
BsaI BsaHI BseYI BsiHKAI BsmI Bsp1286I BsrDI BsrGI
BstAPI BstXI BstYI BtgI EaeI EarI EcoICRI Eco57MI
EcoRV FalI Hin4I HincII HindIII HpaI MscI MslI
MspA1I NcoI NspI PciI PfoI PshAI PsiI PvuII
SacI ScaI SfcI SspI StyI TaqII TatI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BanI BbeI BbvCI BcgI BfrBI BglII
BmgBI BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaWI
BsaXI BseRI BsgI BsiEI BsiWI BsmBI BspEI BspHI
BspMI BsrBI BsrFI BssHII BssSI BstBI BstEII BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoNI EcoO109I EcoRI FseI FspI FspAI
HaeII KasI KpnI MfeI MluI MmeI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PflMI PmeI PmlI PpiI PpuMI PspOMI PspXI PsrI
PstI PvuI RsrII SacII SalI SanDI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI StuI SwaI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI