(Circular) (Six-base) MAPSORT of: pFN21AB8665.seq Check: 5297 from: 1 to: 6202 pFN21AB8665 6202 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3010 3010 Size: 6202 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4012 278 Size: 53 83 186 936 2476 2468 Fragments arranged by size: 2476 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2795 3545 1937 Size: 858 750 4594 Fragments arranged by size: 4594 858 750 Acc65I G'GTAC_C Cuts at: 3078 3523 3078 Size: 445 5757 AclI AA'CG_TT Cuts at: 1563 4329 4702 1563 Size: 2766 373 3063 Fragments arranged by size: 3063 2766 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2220 2984 4265 5649 5917 1632 Size: 588 764 1281 1384 268 1917 Fragments arranged by size: 1917 1384 1281 764 588 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6005 AflIII A'CryG_T Cuts at: 1203 5101 6078 1203 Size: 3898 977 1327 Fragments arranged by size: 3898 1327 977 AgeI A'CCGG_T Cuts at: 5033 5033 Size: 6202 AhdI GACnn_n'nnGTC Cuts at: 4931 4931 Size: 6202 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6202 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2768 1184 Size: 1584 4618 AlwNI CAG_nnn'CTG Cuts at: 2539 2851 3382 3945 5517 2539 Size: 312 531 563 1572 3224 Fragments arranged by size: 3224 1572 563 531 312 ApaI G_GGCC'C Cuts at: 2767 2767 Size: 6202 ApaLI G'TGCA_C Cuts at: 2899 4259 5415 2899 Size: 1360 1156 3686 Fragments arranged by size: 3686 1360 1156 ApoI r'AATT_y Cuts at: 1493 2984 3511 3737 3889 5998 1493 Size: 1491 527 226 152 2109 1697 Fragments arranged by size: 2109 1697 1491 527 226 152 AseI AT'TA_AT Cuts at: 160 1032 4756 160 Size: 872 3724 1606 Fragments arranged by size: 3724 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6202 AvaI C'yCGr_G Cuts at: 1870 1943 2759 3340 3527 1870 Size: 73 816 581 187 4545 Fragments arranged by size: 4545 816 581 187 73 BamHI G'GATC_C Cuts at: 3532 3899 3532 Size: 367 5835 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2734 3078 3523 4978 618 Size: 325 217 627 947 344 445 1455 1842 Fragments arranged by size: 1842 1455 947 627 445 344 325 217 BanII G_rGCy'C Cuts at: 1391 2025 2767 3521 1391 Size: 634 742 754 4072 Fragments arranged by size: 4072 754 742 634 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5231 BbvCI CC'TCA_GC Cuts at: 3423 3423 Size: 6202 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4393 4427 1903 Size: 34 2456 34 3678 Fragments arranged by size: 3678 2456 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4094 5304 4094 Size: 1210 4992 BclI T'GATC_A Cuts at: 1548 1548 Size: 6202 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3201 3254 4813 Size: 107 122 71 631 605 1529 53 1559 Cuts at: 4813 136 Size: 1525 Fragments arranged by size: 1559 1529 1525 631 605 122 107 71 53 BglII A'GATC_T Cuts at: 6197 6197 Size: 6202 BlpI GC'TnA_GC Cuts at: 3619 3619 Size: 6202 Bme1580I G_kGCm'C Cuts at: 1790 2739 2767 2903 3347 4263 5419 1790 Size: 949 28 136 444 916 1156 2573 Fragments arranged by size: 2573 1156 949 916 444 136 28 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6202 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3933 4891 442 Size: 416 53 83 399 24 2516 958 1753 Fragments arranged by size: 2516 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6202 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2399 2576 2585 2609 3337 3359 3404 3430 Size: 1160 177 9 24 728 22 45 26 Cuts at: 3430 4862 1239 Size: 1432 2579 Fragments arranged by size: 2579 1432 1160 728 177 45 26 24 22 9 Bpu10I CC'TnA_GC Cuts at: 3423 3423 Size: 6202 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3449 4327 5192 5490 3449 Size: 878 865 298 4161 Fragments arranged by size: 4161 878 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4865 882 Size: 617 3366 2219 Fragments arranged by size: 3366 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3993 493 Size: 711 2789 2702 Fragments arranged by size: 2789 2702 711 BsaBI GATnn'nnATC Cuts at: 6196 6196 Size: 6202 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3162 4009 4391 5026 Size: 53 83 186 936 1629 847 382 635 Cuts at: 5026 275 Size: 1451 Fragments arranged by size: 1629 1451 936 847 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2857 3117 4635 5033 5307 5454 5865 2857 Size: 260 1518 398 274 147 411 3194 Fragments arranged by size: 3194 1518 411 398 274 260 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5977 Fragments arranged by size: 5977 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2069 2102 2129 2222 2561 2564 2582 3170 Size: 883 33 27 93 339 3 18 588 Cuts at: 3170 3328 1186 Size: 158 4060 Fragments arranged by size: 4060 883 588 339 158 93 33 27 18 3 BseYI C'CCAG_C Cuts at: 2428 2766 3100 5405 5901 2428 Size: 338 334 2305 496 2729 Fragments arranged by size: 2729 2305 496 338 334 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2129 2864 1163 Size: 573 393 735 4501 Fragments arranged by size: 4501 735 573 393 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2100 2688 3073 3334 3652 4413 Size: 275 186 99 588 385 261 318 761 Cuts at: 4413 5441 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 761 588 385 318 275 261 186 99 BsiHKAI G_wGCw'C Cuts at: 2025 2903 2939 4263 4348 5419 2025 Size: 878 36 1324 85 1071 2808 Fragments arranged by size: 2808 1324 1071 878 85 36 BsmI GAATG_Cn' Cuts at: 3713 3806 3713 Size: 93 6109 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2025 2739 2767 2903 2939 3347 3521 Size: 399 235 714 28 136 36 408 174 Cuts at: 3521 4263 4348 5419 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 714 408 399 235 174 136 85 36 28 BspEI T'CCGG_A Cuts at: 3117 5865 3117 Size: 2748 3454 BspHI T'CATG_A Cuts at: 4091 5821 4091 Size: 1730 4472 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2475 3010 3066 3557 844 Size: 1631 535 56 491 3489 Fragments arranged by size: 3489 1631 535 491 56 BsrBI CCG'CTC Cuts at: 1394 1823 2560 3097 3336 4089 1394 Size: 429 737 537 239 753 3507 Fragments arranged by size: 3507 753 737 537 429 239 BsrDI GCAATG_nn' Cuts at: 65 1248 4697 4871 65 Size: 1183 3449 174 1396 Fragments arranged by size: 3449 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2708 2816 4846 5033 1700 Size: 110 898 108 2030 187 2869 Fragments arranged by size: 2869 2030 898 187 110 108 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6202 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6202 BssSI C'ACGA_G Cuts at: 2930 4262 5274 2930 Size: 1332 1012 3858 Fragments arranged by size: 3858 1332 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2851 794 Size: 2057 4145 BstBI TT'CG_AA Cuts at: 5006 5006 Size: 6202 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6202 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2401 2497 2698 2011 Size: 390 96 201 5515 Fragments arranged by size: 5515 390 201 96 BstYI r'GATC_y Cuts at: 1972 2128 2475 2860 3114 3261 3532 3899 4284 Size: 156 347 385 254 147 271 367 385 Cuts at: 4284 4301 5753 5839 5851 6197 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 385 385 367 347 346 271 254 156 147 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2304 2682 513 Size: 552 1239 378 4033 Fragments arranged by size: 4033 1239 552 378 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2140 2632 487 Size: 683 56 914 492 4057 Fragments arranged by size: 4057 914 683 492 56 BtsI GCAGTG_nn' Cuts at: 3220 3718 4512 4532 3220 Size: 498 794 20 4890 Fragments arranged by size: 4890 794 498 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6202 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6167 DraI TTT'AAA Cuts at: 3506 3506 Size: 6202 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5209 809 Size: 507 3893 1802 Fragments arranged by size: 3893 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2685 2706 2889 Size: 54 1425 50 275 18 855 21 183 Cuts at: 2889 3070 3649 4538 6167 8 Size: 181 579 889 1629 43 Fragments arranged by size: 1629 1425 889 855 579 275 183 181 54 50 43 21 18 EagI C'GGCC_G Cuts at: 1537 1812 2685 3070 3649 1537 Size: 275 873 385 579 4090 Fragments arranged by size: 4090 873 579 385 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2034 2046 2079 3476 4132 1353 Size: 681 12 33 1397 656 3423 Fragments arranged by size: 3423 1397 681 656 33 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2884 4789 5163 5309 387 Size: 1064 138 1295 1905 374 146 1280 Fragments arranged by size: 1905 1295 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2023 2023 Size: 6202 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2220 2399 2576 2585 2609 2984 3337 Size: 393 588 179 177 9 24 375 353 Cuts at: 3337 3359 3404 3430 4265 4862 5649 5917 1239 Size: 22 45 26 835 597 787 268 1524 Fragments arranged by size: 1524 835 787 597 588 393 375 353 268 179 177 45 26 24 22 9 EcoNI CCTnn'n_nnAGG Cuts at: 2729 2729 Size: 6202 EcoO109I rG'GnC_Cy Cuts at: 2763 3175 3379 2763 Size: 412 204 5586 Fragments arranged by size: 5586 412 204 EcoRI G'AATT_C Cuts at: 3511 3889 3511 Size: 378 5824 FspI TGC'GCA Cuts at: 4708 4708 Size: 6202 HaeII r_GCGC'y Cuts at: 1678 2588 5349 1678 Size: 910 2761 2531 Fragments arranged by size: 2761 2531 910 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2342 2374 3323 3355 4843 Size: 32 135 32 770 32 949 32 1488 Cuts at: 4843 4875 4917 4949 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1488 949 770 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3546 3792 669 Size: 988 281 1608 246 3079 Fragments arranged by size: 3079 1608 988 281 246 HindIII A'AGCT_T Cuts at: 748 2232 748 Size: 1484 4718 HpaI GTT'AAC Cuts at: 3792 3792 Size: 6202 KpnI G_GTAC'C Cuts at: 3082 3527 3082 Size: 445 5757 MfeI C'AATT_G Cuts at: 3801 3801 Size: 6202 MluI A'CGCG_T Cuts at: 6078 6078 Size: 6202 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2523 5316 5500 1305 Size: 1218 2793 184 2007 Fragments arranged by size: 2793 2007 1218 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4380 Fragments arranged by size: 4380 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2495 2702 4160 4519 4678 518 Size: 648 1329 207 1458 359 159 2042 Fragments arranged by size: 2042 1458 1329 648 359 207 159 MspA1I CmG'CkG Cuts at: 1606 2125 2539 3031 3055 3280 3618 4295 5443 Size: 519 414 492 24 225 338 677 1148 Cuts at: 5443 5688 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 519 492 414 338 245 225 24 NaeI GCC'GGC Cuts at: 2710 2710 Size: 6202 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5650 NdeI CA'TA_TG Cuts at: 387 387 Size: 6202 NgoMIV G'CCGG_C Cuts at: 2708 2708 Size: 6202 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6202 NotI GC'GGCC_GC Cuts at: 3070 3649 3070 Size: 579 5623 NspI r_CATG'y Cuts at: 1132 1229 3560 5105 1132 Size: 97 2331 1545 2229 Fragments arranged by size: 2331 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2527 1348 Size: 1179 5023 PciI A'CATG_T Cuts at: 5101 5101 Size: 6202 PflMI CCAn_nnn'nTGG Cuts at: 1108 2166 3382 1108 Size: 1058 1216 3928 Fragments arranged by size: 3928 1216 1058 PfoI T'CCnGG_A Cuts at: 2346 2633 2346 Size: 287 5915 PmeI GTTT'AAAC Cuts at: 3506 3506 Size: 6202 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4271 4303 5784 5816 4271 Size: 32 1481 32 4657 Fragments arranged by size: 4657 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3175 3175 Size: 6202 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6202 PsiI TTA'TAA Cuts at: 3772 3772 Size: 6202 PspOMI G'GGCC_C Cuts at: 2763 2763 Size: 6202 PstI C_TGCA'G Cuts at: 830 2472 3279 3291 3554 830 Size: 1642 807 12 263 3478 Fragments arranged by size: 3478 1642 807 263 12 PvuI CG_AT'CG Cuts at: 2001 2100 2001 Size: 99 6103 PvuII CAG'CTG Cuts at: 2125 2539 2125 Size: 414 5788 SacI G_AGCT'C Cuts at: 2025 2025 Size: 6202 SalI G'TCGA_C Cuts at: 1936 3544 1936 Size: 1608 4594 SbfI CC_TGCA'GG Cuts at: 3554 3554 Size: 6202 ScaI AGT'ACT Cuts at: 2357 4450 6162 2357 Size: 2093 1712 2397 Fragments arranged by size: 2397 2093 1712 SfcI C'TryA_G Cuts at: 826 1045 2468 3275 3287 3550 4685 5366 5557 Size: 219 1423 807 12 263 1135 681 191 Cuts at: 5557 826 Size: 1471 Fragments arranged by size: 1471 1423 1135 807 681 263 219 191 12 SfiI GGCCn_nnn'nGGCC Cuts at: 3254 3254 Size: 6202 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6202 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6202 SmaI CCC'GGG Cuts at: 1872 3529 1872 Size: 1657 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3464 4306 5207 5469 820 Size: 197 926 1521 842 901 262 1553 Fragments arranged by size: 1553 1521 926 901 842 262 197 SnaBI TAC'GTA Cuts at: 493 3993 493 Size: 3500 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6202 SphI G_CATG'C Cuts at: 1132 3560 1132 Size: 2428 3774 SspI AAT'ATT Cuts at: 5 52 4126 5 Size: 47 4074 2081 Fragments arranged by size: 4074 2081 47 StuI AGG'CCT Cuts at: 1517 2573 1517 Size: 1056 5146 StyI C'CwwG_G Cuts at: 513 1065 2385 2730 3252 3641 513 Size: 552 1320 345 522 389 3074 Fragments arranged by size: 3074 1320 552 522 389 345 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2540 2668 2690 4229 4246 4399 4584 Size: 1255 960 128 22 1539 17 153 185 Cuts at: 4584 5029 325 Size: 445 1498 Fragments arranged by size: 1539 1498 1255 960 445 185 153 128 22 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2355 4448 6160 Size: 274 80 33 51 1443 378 2093 1712 Cuts at: 6160 96 Size: 138 Fragments arranged by size: 2093 1712 1443 378 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6162 6194 6162 Size: 32 6170 Tth111I GACn'n_nGTC Cuts at: 1316 2779 1316 Size: 1463 4739 XbaI T'CTAG_A Cuts at: 3538 3538 Size: 6202 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2902 2902 Size: 6202 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6202 XmaI C'CCGG_G Cuts at: 1870 3527 1870 Size: 1657 4545 XmnI GAAnn'nnTTC Cuts at: 3440 4331 3440 Size: 891 5311 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4010 276 Size: 53 83 186 936 2476 2468 Fragments arranged by size: 2476 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfoI SrfI SwaI