(Circular) (Six-base) MAPSORT of: pFN21AB8660.seq Check: 5769 from: 1 to: 6352 pFN21AB8660 6352 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2951 4162 278 Size: 53 83 186 936 1415 1211 2468 Fragments arranged by size: 2468 1415 1211 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3695 1937 Size: 1758 4594 Acc65I G'GTAC_C Cuts at: 3673 3673 Size: 6352 AclI AA'CG_TT Cuts at: 1563 4479 4852 1563 Size: 2916 373 3063 Fragments arranged by size: 3063 2916 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2464 3544 4415 5799 6067 1632 Size: 832 1080 871 1384 268 1917 Fragments arranged by size: 1917 1384 1080 871 832 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6155 AflIII A'CryG_T Cuts at: 1203 2865 5251 6228 1203 Size: 1662 2386 977 1327 Fragments arranged by size: 2386 1662 1327 977 AgeI A'CCGG_T Cuts at: 2982 5183 2982 Size: 2201 4151 AhdI GACnn_n'nnGTC Cuts at: 5081 5081 Size: 6352 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6352 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2992 1184 Size: 1808 4544 AlwNI CAG_nnn'CTG Cuts at: 2644 2893 4095 5667 2644 Size: 249 1202 1572 3329 Fragments arranged by size: 3329 1572 1202 249 ApaI G_GGCC'C Cuts at: 2330 3404 2330 Size: 1074 5278 ApaLI G'TGCA_C Cuts at: 4409 5565 4409 Size: 1156 5196 ApoI r'AATT_y Cuts at: 1493 2205 3661 3887 4039 6148 1493 Size: 712 1456 226 152 2109 1697 Fragments arranged by size: 2109 1697 1456 712 226 152 AseI AT'TA_AT Cuts at: 160 1032 4906 160 Size: 872 3874 1606 Fragments arranged by size: 3874 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6352 AvaI C'yCGr_G Cuts at: 1870 1943 2840 3092 3677 1870 Size: 73 897 252 585 4545 Fragments arranged by size: 4545 897 585 252 73 BamHI G'GATC_C Cuts at: 3682 4049 3682 Size: 367 5985 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3229 3551 3673 5128 618 Size: 325 217 627 1442 322 122 1455 1842 Fragments arranged by size: 1842 1455 1442 627 325 322 217 122 BanII G_rGCy'C Cuts at: 1391 2330 2709 2847 3404 3671 1391 Size: 939 379 138 557 267 4072 Fragments arranged by size: 4072 939 557 379 267 138 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2951 3149 3279 928 Size: 971 1052 198 130 4001 Fragments arranged by size: 4001 1052 971 198 130 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2968 3002 4543 4577 1903 Size: 34 1031 34 1541 34 3678 Fragments arranged by size: 3678 1541 1031 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4244 5454 4244 Size: 1210 5142 BclI T'GATC_A Cuts at: 1548 1548 Size: 6352 BfrBI ATG'CAT Cuts at: 2621 2621 Size: 6352 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2688 3612 4963 Size: 107 122 71 631 605 1016 924 1351 Cuts at: 4963 136 Size: 1525 Fragments arranged by size: 1525 1351 1016 924 631 605 122 107 71 BglII A'GATC_T Cuts at: 2776 6347 2776 Size: 3571 2781 BlpI GC'TnA_GC Cuts at: 3769 3769 Size: 6352 Bme1580I G_kGCm'C Cuts at: 1790 2041 2330 2632 2971 3232 3293 3306 3404 Size: 251 289 302 339 261 61 13 98 Cuts at: 3404 3554 4413 5569 1790 Size: 150 859 1156 2573 Fragments arranged by size: 2573 1156 859 339 302 289 261 251 150 98 61 13 BmgBI CAC'GTC Cuts at: 1318 2011 2739 2866 1318 Size: 693 728 127 4804 Fragments arranged by size: 4804 728 693 127 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2266 4083 5041 Size: 416 53 83 399 24 849 1817 958 Cuts at: 5041 442 Size: 1753 Fragments arranged by size: 1817 1753 958 849 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6352 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2677 2677 Size: 6352 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3159 3364 3627 5012 1239 Size: 1920 205 263 1385 2579 Fragments arranged by size: 2579 1920 1385 263 205 Bpu10I CC'TnA_GC Cuts at: 2112 2573 3321 2112 Size: 461 748 5143 Fragments arranged by size: 5143 748 461 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3008 4477 5342 5640 3008 Size: 1469 865 298 3720 Fragments arranged by size: 3720 1469 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5015 882 Size: 617 3516 2219 Fragments arranged by size: 3516 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3134 4143 493 Size: 711 1930 1009 2702 Fragments arranged by size: 2702 1930 1009 711 BsaBI GATnn'nnATC Cuts at: 6346 6346 Size: 6352 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2948 4159 4541 5176 Size: 53 83 186 936 1415 1211 382 635 Cuts at: 5176 275 Size: 1451 Fragments arranged by size: 1451 1415 1211 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2814 2982 4785 5183 5457 5604 6015 2814 Size: 168 1803 398 274 147 411 3151 Fragments arranged by size: 3151 1803 411 398 274 168 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6127 Fragments arranged by size: 6127 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2169 2552 2588 2627 1186 Size: 983 383 36 39 4911 Fragments arranged by size: 4911 983 383 39 36 BseYI C'CCAG_C Cuts at: 2087 2248 3082 3305 3438 3530 5555 6051 2087 Size: 161 834 223 133 92 2025 496 2388 Fragments arranged by size: 2388 2025 834 496 223 161 133 92 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2657 3510 1163 Size: 573 921 853 4005 Fragments arranged by size: 4005 921 853 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2443 3802 4563 5591 1540 Size: 275 186 442 1359 761 1028 2301 Fragments arranged by size: 2301 1359 1028 761 442 275 186 BsiHKAI G_wGCw'C Cuts at: 2427 3024 3589 4413 4498 5569 2427 Size: 597 565 824 85 1071 3210 Fragments arranged by size: 3210 1071 824 597 565 85 BsmI GAATG_Cn' Cuts at: 2313 2621 3863 3956 2313 Size: 308 1242 93 4709 Fragments arranged by size: 4709 1242 308 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2159 2159 Size: 6352 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2041 2330 2427 2632 2709 2847 2971 Size: 399 251 289 97 205 77 138 124 Cuts at: 2971 3024 3232 3293 3306 3404 3554 3589 3671 Size: 53 208 61 13 98 150 35 82 Cuts at: 3671 4413 4498 5569 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 289 251 208 205 150 138 124 98 97 85 82 77 61 53 35 13 BspEI T'CCGG_A Cuts at: 2814 6015 2814 Size: 3201 3151 BspHI T'CATG_A Cuts at: 2394 4241 5971 2394 Size: 1847 1730 2775 Fragments arranged by size: 2775 1847 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3081 3707 844 Size: 2237 626 3489 Fragments arranged by size: 3489 2237 626 BsrBI CCG'CTC Cuts at: 1394 1823 3505 4239 1394 Size: 429 1682 734 3507 Fragments arranged by size: 3507 1682 734 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4847 5021 65 Size: 1183 3599 174 1396 Fragments arranged by size: 3599 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2982 3124 4996 5183 1700 Size: 110 1172 142 1872 187 2869 Fragments arranged by size: 2869 1872 1172 187 142 110 BsrGI T'GTAC_A Cuts at: 96 2158 96 Size: 2062 4290 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6352 BssSI C'ACGA_G Cuts at: 4412 5424 4412 Size: 1012 5340 BstAPI GCAn_nnn'nTGC Cuts at: 794 2665 794 Size: 1871 4481 BstBI TT'CG_AA Cuts at: 2122 5156 2122 Size: 3034 3318 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6352 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2755 2011 Size: 744 5608 BstYI r'GATC_y Cuts at: 1972 2776 2832 3682 4049 4434 4451 5903 5989 Size: 804 56 850 367 385 17 1452 86 Cuts at: 5989 6001 6347 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 850 804 385 367 346 86 56 17 12 Bsu36I CC'TnA_GG Cuts at: 2610 3333 3595 2610 Size: 723 262 5367 Fragments arranged by size: 5367 723 262 BtgI C'CryG_G Cuts at: 513 1065 2004 2754 2963 3197 3224 513 Size: 552 939 750 209 234 27 3641 Fragments arranged by size: 3641 939 750 552 234 209 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2566 487 Size: 683 56 1340 4273 Fragments arranged by size: 4273 1340 683 56 BtsI GCAGTG_nn' Cuts at: 2795 3362 3587 3868 4662 4682 2795 Size: 567 225 281 794 20 4465 Fragments arranged by size: 4465 794 567 281 225 20 ClaI AT'CG_AT Cuts at: 1554 2488 1554 Size: 934 5418 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6317 DraI TTT'AAA Cuts at: 3656 3656 Size: 6352 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5359 809 Size: 507 4043 1802 Fragments arranged by size: 4043 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2646 3799 4688 Size: 54 1425 50 275 18 816 1153 889 Cuts at: 4688 6317 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1153 889 816 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3799 1537 Size: 275 1987 4090 Fragments arranged by size: 4090 1987 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4282 1353 Size: 2929 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3401 4939 5313 5459 387 Size: 1064 138 1812 1538 374 146 1280 Fragments arranged by size: 1812 1538 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2464 3159 3364 3544 3627 4415 5012 Size: 393 832 695 205 180 83 788 597 Cuts at: 5012 5799 6067 1239 Size: 787 268 1524 Fragments arranged by size: 1524 832 788 787 695 597 393 268 205 180 83 EcoNI CCTnn'n_nnAGG Cuts at: 3001 3152 3320 3001 Size: 151 168 6033 Fragments arranged by size: 6033 168 151 EcoO109I rG'GnC_Cy Cuts at: 2108 2326 2569 3036 3070 3169 3329 3400 3401 Size: 218 243 467 34 99 160 71 1 Cuts at: 3401 3599 2108 Size: 198 4861 Fragments arranged by size: 4861 467 243 218 198 160 99 71 34 1 EcoRI G'AATT_C Cuts at: 3661 4039 3661 Size: 378 5974 FspI TGC'GCA Cuts at: 4858 4858 Size: 6352 HaeII r_GCGC'y Cuts at: 1678 2894 2938 5499 1678 Size: 1216 44 2561 2531 Fragments arranged by size: 2561 2531 1216 44 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2040 2072 2376 2408 2819 Size: 32 135 32 468 32 304 32 411 Cuts at: 2819 2851 3086 3118 4993 5025 5067 5099 1373 Size: 32 235 32 1875 32 42 32 2626 Fragments arranged by size: 2626 1875 468 411 304 235 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3696 3942 669 Size: 988 281 1758 246 3079 Fragments arranged by size: 3079 1758 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6352 HpaI GTT'AAC Cuts at: 3942 3942 Size: 6352 KpnI G_GTAC'C Cuts at: 3677 3677 Size: 6352 MfeI C'AATT_G Cuts at: 3951 3951 Size: 6352 MluI A'CGCG_T Cuts at: 6228 6228 Size: 6352 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3567 5466 5650 1305 Size: 2262 1899 184 2007 Fragments arranged by size: 2262 2007 1899 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2648 10 Size: 54 1425 343 816 3714 Fragments arranged by size: 3714 1425 816 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2060 4310 4669 4828 518 Size: 648 843 51 2250 359 159 2042 Fragments arranged by size: 2250 2042 843 648 359 159 51 MspA1I CmG'CkG Cuts at: 1606 2506 2641 3199 3469 3768 4445 5593 5838 Size: 900 135 558 270 299 677 1148 245 Cuts at: 5838 1606 Size: 2120 Fragments arranged by size: 2120 1148 900 677 558 299 270 245 135 NaeI GCC'GGC Cuts at: 3126 3126 Size: 6352 NcoI C'CATG_G Cuts at: 513 1065 2004 2754 513 Size: 552 939 750 4111 Fragments arranged by size: 4111 939 750 552 NdeI CA'TA_TG Cuts at: 387 2533 387 Size: 2146 4206 NgoMIV G'CCGG_C Cuts at: 3124 3124 Size: 6352 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6352 NotI GC'GGCC_GC Cuts at: 3799 3799 Size: 6352 NsiI A_TGCA'T Cuts at: 2623 2623 Size: 6352 NspI r_CATG'y Cuts at: 1132 1229 2729 3710 5255 1132 Size: 97 1500 981 1545 2229 Fragments arranged by size: 2229 1545 1500 981 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6352 PciI A'CATG_T Cuts at: 5251 5251 Size: 6352 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6352 PfoI T'CCnGG_A Cuts at: 3267 3341 3267 Size: 74 6278 PmeI GTTT'AAAC Cuts at: 3656 3656 Size: 6352 PmlI CAC'GTG Cuts at: 3134 3134 Size: 6352 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4421 4453 5934 5966 4421 Size: 32 1481 32 4807 Fragments arranged by size: 4807 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2569 3070 3329 2569 Size: 501 259 5592 Fragments arranged by size: 5592 501 259 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6352 PsiI TTA'TAA Cuts at: 3922 3922 Size: 6352 PspOMI G'GGCC_C Cuts at: 2326 3400 2326 Size: 1074 5278 PstI C_TGCA'G Cuts at: 830 2567 2670 3357 3704 830 Size: 1737 103 687 347 3478 Fragments arranged by size: 3478 1737 687 347 103 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6352 PvuII CAG'CTG Cuts at: 2641 3469 2641 Size: 828 5524 SacII CC_GC'GG Cuts at: 3200 3200 Size: 6352 SalI G'TCGA_C Cuts at: 1936 3694 1936 Size: 1758 4594 SbfI CC_TGCA'GG Cuts at: 2670 3704 2670 Size: 1034 5318 ScaI AGT'ACT Cuts at: 4600 6312 4600 Size: 1712 4640 SfcI C'TryA_G Cuts at: 826 1045 2563 2666 3353 3700 4835 5516 5707 Size: 219 1518 103 687 347 1135 681 191 Cuts at: 5707 826 Size: 1471 Fragments arranged by size: 1518 1471 1135 687 681 347 219 191 103 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6352 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6352 SmaI CCC'GGG Cuts at: 1872 3679 1872 Size: 1807 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3023 4456 5357 5619 820 Size: 197 926 1080 1433 901 262 1553 Fragments arranged by size: 1553 1433 1080 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4143 493 Size: 3650 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6352 SphI G_CATG'C Cuts at: 1132 3710 1132 Size: 2578 3774 SspI AAT'ATT Cuts at: 5 52 4276 5 Size: 47 4224 2081 Fragments arranged by size: 4224 2081 47 StuI AGG'CCT Cuts at: 1517 3118 1517 Size: 1601 4751 StyI C'CwwG_G Cuts at: 513 1065 2004 2754 3404 3791 513 Size: 552 939 750 650 387 3074 Fragments arranged by size: 3074 939 750 650 552 387 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2227 3544 4379 4396 4549 4734 5179 Size: 1255 647 1317 835 17 153 185 445 Cuts at: 5179 325 Size: 1498 Fragments arranged by size: 1498 1317 1255 835 647 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2158 2541 4598 Size: 274 80 33 51 1443 181 383 2057 Cuts at: 4598 6310 96 Size: 1712 138 Fragments arranged by size: 2057 1712 1443 383 274 181 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3235 3267 6312 6344 3235 Size: 32 3045 32 3243 Fragments arranged by size: 3243 3045 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6352 XbaI T'CTAG_A Cuts at: 3688 3688 Size: 6352 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3313 3313 Size: 6352 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6352 XmaI C'CCGG_G Cuts at: 1870 3677 1870 Size: 1807 4545 XmnI GAAnn'nnTTC Cuts at: 4481 4481 Size: 6352 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2949 4160 276 Size: 53 83 186 936 1415 1211 2468 Fragments arranged by size: 2468 1415 1211 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BsiWI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI PacI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI