(Circular) (Six-base) MAPSORT of: pFN21AB8658.seq Check: 4037 from: 1 to: 6265 pFN21AB8658 6265 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3565 3565 Size: 6265 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3433 4075 278 Size: 53 83 186 936 1897 642 2468 Fragments arranged by size: 2468 1897 936 642 186 83 53 AccI GT'mk_AC Cuts at: 1937 2376 2829 3427 3608 1937 Size: 439 453 598 181 4594 Fragments arranged by size: 4594 598 453 439 181 Acc65I G'GTAC_C Cuts at: 3586 3586 Size: 6265 AclI AA'CG_TT Cuts at: 1563 4392 4765 1563 Size: 2829 373 3063 Fragments arranged by size: 3063 2829 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2844 4328 5712 5980 1632 Size: 1212 1484 1384 268 1917 Fragments arranged by size: 1917 1484 1384 1212 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6068 AflIII A'CryG_T Cuts at: 1203 5164 6141 1203 Size: 3961 977 1327 Fragments arranged by size: 3961 1327 977 AgeI A'CCGG_T Cuts at: 5096 5096 Size: 6265 AhdI GACnn_n'nnGTC Cuts at: 4994 4994 Size: 6265 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6265 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6265 AlwNI CAG_nnn'CTG Cuts at: 2309 4008 5580 2309 Size: 1699 1572 2994 Fragments arranged by size: 2994 1699 1572 ApaLI G'TGCA_C Cuts at: 4322 5478 4322 Size: 1156 5109 ApoI r'AATT_y Cuts at: 1493 3574 3800 3952 6061 1493 Size: 2081 226 152 2109 1697 Fragments arranged by size: 2109 2081 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 2400 4819 160 Size: 872 1368 2419 1606 Fragments arranged by size: 2419 1606 1368 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6265 AvaI C'yCGr_G Cuts at: 1870 1943 3590 1870 Size: 73 1647 4545 Fragments arranged by size: 4545 1647 73 BamHI G'GATC_C Cuts at: 3595 3962 3595 Size: 367 5898 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3586 5041 618 Size: 325 217 627 1799 1455 1842 Fragments arranged by size: 1842 1799 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2875 3584 1391 Size: 1484 709 4072 Fragments arranged by size: 4072 1484 709 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3402 928 Size: 971 1503 3791 Fragments arranged by size: 3791 1503 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4456 4490 1903 Size: 34 2519 34 3678 Fragments arranged by size: 3678 2519 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2535 4157 5367 2535 Size: 1622 1210 3433 Fragments arranged by size: 3433 1622 1210 BclI T'GATC_A Cuts at: 1548 3250 1548 Size: 1702 4563 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4876 136 Size: 107 122 71 631 605 337 2867 1525 Fragments arranged by size: 2867 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2813 6260 2813 Size: 3447 2818 BlpI GC'TnA_GC Cuts at: 2042 3682 2042 Size: 1640 4625 Bme1580I G_kGCm'C Cuts at: 1790 4326 5482 1790 Size: 2536 1156 2573 Fragments arranged by size: 2573 2536 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6265 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2697 3996 4954 Size: 416 53 83 399 24 1280 1299 958 Cuts at: 4954 442 Size: 1753 Fragments arranged by size: 1753 1299 1280 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6265 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2429 2429 Size: 6265 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2850 4925 1239 Size: 1611 2075 2579 Fragments arranged by size: 2579 2075 1611 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4390 5255 5553 4390 Size: 865 298 5102 Fragments arranged by size: 5102 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2192 3189 4928 882 Size: 617 693 997 1739 2219 Fragments arranged by size: 2219 1739 997 693 617 BsaAI yAC'GTr Cuts at: 493 1204 3047 4056 493 Size: 711 1843 1009 2702 Fragments arranged by size: 2702 1843 1009 711 BsaBI GATnn'nnATC Cuts at: 6259 6259 Size: 6265 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3430 4072 4454 5089 Size: 53 83 186 936 1897 642 382 635 Cuts at: 5089 275 Size: 1451 Fragments arranged by size: 1897 1451 936 642 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2054 4698 5096 5370 5517 5928 2054 Size: 2644 398 274 147 411 2391 Fragments arranged by size: 2644 2391 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2060 2090 2284 2314 2653 Size: 30 165 30 657 30 194 30 339 Cuts at: 2653 2683 1178 Size: 30 4760 Fragments arranged by size: 4760 657 339 194 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2081 2658 2865 2886 2904 2955 1186 Size: 895 577 207 21 18 51 4496 Fragments arranged by size: 4496 895 577 207 51 21 18 BseYI C'CCAG_C Cuts at: 5468 5964 5468 Size: 496 5769 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3027 1163 Size: 573 1291 4401 Fragments arranged by size: 4401 1291 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2013 3715 4476 5504 1540 Size: 275 186 12 1702 761 1028 2301 Fragments arranged by size: 2301 1702 1028 761 275 186 12 BsiHKAI G_wGCw'C Cuts at: 2281 2875 3545 4326 4411 5482 2281 Size: 594 670 781 85 1071 3064 Fragments arranged by size: 3064 1071 781 670 594 85 BsmI GAATG_Cn' Cuts at: 3776 3869 3776 Size: 93 6172 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2281 2875 3545 3584 4326 4411 5482 Size: 399 491 594 670 39 742 85 1071 Cuts at: 5482 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 670 594 491 399 85 39 BspEI T'CCGG_A Cuts at: 5928 5928 Size: 6265 BspHI T'CATG_A Cuts at: 4154 5884 4154 Size: 1730 4535 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3565 3620 844 Size: 2721 55 3489 Fragments arranged by size: 3489 2721 55 BsrBI CCG'CTC Cuts at: 1394 1823 2046 4152 1394 Size: 429 223 2106 3507 Fragments arranged by size: 3507 2106 429 223 BsrDI GCAATG_nn' Cuts at: 65 1248 3070 4760 4934 65 Size: 1183 1822 1690 174 1396 Fragments arranged by size: 1822 1690 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2012 4909 5096 1700 Size: 110 202 2897 187 2869 Fragments arranged by size: 2897 2869 202 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6265 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6265 BssSI C'ACGA_G Cuts at: 4325 5337 4325 Size: 1012 5253 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6265 BstBI TT'CG_AA Cuts at: 5069 5069 Size: 6265 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6265 BstXI CCAn_nnnn'nTGG Cuts at: 3086 3086 Size: 6265 BstYI r'GATC_y Cuts at: 1972 2813 3595 3962 4347 4364 5816 5902 5914 Size: 841 782 367 385 17 1452 86 12 Cuts at: 5914 6260 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 841 782 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2450 513 Size: 552 1385 4328 Fragments arranged by size: 4328 1385 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2103 2241 3297 487 Size: 683 56 877 138 1056 3455 Fragments arranged by size: 3455 1056 877 683 138 56 BtsI GCAGTG_nn' Cuts at: 2928 3137 3523 3781 4575 4595 2928 Size: 209 386 258 794 20 4598 Fragments arranged by size: 4598 794 386 258 209 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6265 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6230 DraI TTT'AAA Cuts at: 3569 3569 Size: 6265 DraIII CAC_nnn'GTG Cuts at: 3269 3269 Size: 6265 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5272 809 Size: 507 3956 1802 Fragments arranged by size: 3956 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2010 3712 4601 Size: 54 1425 50 275 18 180 1702 889 Cuts at: 4601 6230 8 Size: 1629 43 Fragments arranged by size: 1702 1629 1425 889 275 180 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2010 3712 1537 Size: 275 198 1702 4090 Fragments arranged by size: 4090 1702 275 198 EarI CTCTTCn'nnn_ Cuts at: 1353 2067 2332 3145 4195 1353 Size: 714 265 813 1050 3423 Fragments arranged by size: 3423 1050 813 714 265 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4852 5226 5372 387 Size: 1064 138 3263 374 146 1280 Fragments arranged by size: 3263 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2873 2873 Size: 6265 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2844 2850 4328 4925 5712 5980 1239 Size: 393 1212 6 1478 597 787 268 1524 Fragments arranged by size: 1524 1478 1212 787 597 393 268 6 EcoNI CCTnn'n_nnAGG Cuts at: 2970 2970 Size: 6265 EcoO109I rG'GnC_Cy Cuts at: 2800 2800 Size: 6265 EcoRI G'AATT_C Cuts at: 3574 3952 3574 Size: 378 5887 FspI TGC'GCA Cuts at: 4771 4771 Size: 6265 HaeII r_GCGC'y Cuts at: 1678 2029 2979 5412 1678 Size: 351 950 2433 2531 Fragments arranged by size: 2531 2433 950 351 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4906 4938 4980 5012 1373 Size: 32 135 32 3334 32 42 32 2626 Fragments arranged by size: 3334 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3609 3855 669 Size: 988 281 1671 246 3079 Fragments arranged by size: 3079 1671 988 281 246 HindIII A'AGCT_T Cuts at: 748 2517 748 Size: 1769 4496 HpaI GTT'AAC Cuts at: 3855 3855 Size: 6265 KpnI G_GTAC'C Cuts at: 3590 3590 Size: 6265 MfeI C'AATT_G Cuts at: 2721 3864 2721 Size: 1143 5122 MluI A'CGCG_T Cuts at: 6141 6141 Size: 6265 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2016 2942 5379 5563 1305 Size: 711 926 2437 184 2007 Fragments arranged by size: 2437 2007 926 711 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4443 Fragments arranged by size: 4443 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3441 4223 4582 4741 518 Size: 648 2275 782 359 159 2042 Fragments arranged by size: 2275 2042 782 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3681 4358 5506 5751 1606 Size: 2075 677 1148 245 2120 Fragments arranged by size: 2120 2075 1148 677 245 NaeI GCC'GGC Cuts at: 2014 2014 Size: 6265 NcoI C'CATG_G Cuts at: 513 1065 2450 513 Size: 552 1385 4328 Fragments arranged by size: 4328 1385 552 NdeI CA'TA_TG Cuts at: 387 3058 387 Size: 2671 3594 NgoMIV G'CCGG_C Cuts at: 2012 2012 Size: 6265 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6265 NotI GC'GGCC_GC Cuts at: 3712 3712 Size: 6265 NspI r_CATG'y Cuts at: 1132 1229 3623 5168 1132 Size: 97 2394 1545 2229 Fragments arranged by size: 2394 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6265 PciI A'CATG_T Cuts at: 5164 5164 Size: 6265 PflMI CCAn_nnn'nTGG Cuts at: 1108 2288 1108 Size: 1180 5085 PfoI T'CCnGG_A Cuts at: 2866 2866 Size: 6265 PmeI GTTT'AAAC Cuts at: 3569 3569 Size: 6265 PmlI CAC'GTG Cuts at: 3047 3047 Size: 6265 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4334 4366 5847 5879 4334 Size: 32 1481 32 4720 Fragments arranged by size: 4720 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2800 2800 Size: 6265 PshAI GACnn'nnGTC Cuts at: 1613 2363 3406 1613 Size: 750 1043 4472 Fragments arranged by size: 4472 1043 750 PsiI TTA'TAA Cuts at: 3835 3835 Size: 6265 PstI C_TGCA'G Cuts at: 830 3562 3617 830 Size: 2732 55 3478 Fragments arranged by size: 3478 2732 55 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6265 SacI G_AGCT'C Cuts at: 2875 2875 Size: 6265 SalI G'TCGA_C Cuts at: 1936 3607 1936 Size: 1671 4594 SbfI CC_TGCA'GG Cuts at: 3617 3617 Size: 6265 ScaI AGT'ACT Cuts at: 4513 6225 4513 Size: 1712 4553 SexAI A'CCwGG_T Cuts at: 2461 2461 Size: 6265 SfcI C'TryA_G Cuts at: 826 1045 2256 2352 3558 3613 4748 5429 5620 Size: 219 1211 96 1206 55 1135 681 191 Cuts at: 5620 826 Size: 1471 Fragments arranged by size: 1471 1211 1206 1135 681 219 191 96 55 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6265 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6265 SmaI CCC'GGG Cuts at: 1872 3592 1872 Size: 1720 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4369 5270 5532 820 Size: 197 926 2426 901 262 1553 Fragments arranged by size: 2426 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4056 493 Size: 3563 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6265 SphI G_CATG'C Cuts at: 1132 3623 1132 Size: 2491 3774 SspI AAT'ATT Cuts at: 5 52 4189 5 Size: 47 4137 2081 Fragments arranged by size: 4137 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6265 StyI C'CwwG_G Cuts at: 513 1065 2450 3704 513 Size: 552 1385 1254 3074 Fragments arranged by size: 3074 1385 1254 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2216 2723 3177 4292 4309 4462 4647 Size: 1255 636 507 454 1115 17 153 185 Cuts at: 4647 5092 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1115 636 507 454 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4511 6223 96 Size: 274 80 33 51 1443 2534 1712 138 Fragments arranged by size: 2534 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2060 2092 2872 2904 6225 6257 2060 Size: 32 780 32 3321 32 2068 Fragments arranged by size: 3321 2068 780 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6265 XbaI T'CTAG_A Cuts at: 3601 3601 Size: 6265 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6265 XmaI C'CCGG_G Cuts at: 1870 3590 1870 Size: 1720 4545 XmnI GAAnn'nnTTC Cuts at: 4394 4394 Size: 6265 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3431 4073 276 Size: 53 83 186 936 1897 642 2468 Fragments arranged by size: 2468 1897 936 642 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI