Restriction map for pFN21AB8658
 (Circular) (Six-base) MAPSORT of: pFN21AB8658.seq  Check: 4037  from: 1  to: 6265



pFN21AB8658  6265 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:27  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   3565    3565 
   Size:       6265
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3433    4075     278 
   Size:         53      83     186     936    1897     642    2468

  Fragments arranged by size: 

                2468    1897     936     642     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2376    2829    3427    3608    1937 
   Size:        439     453     598     181    4594

  Fragments arranged by size: 

                4594     598     453     439     181
 
Acc65I G'GTAC_C

Cuts at:   3586    3586 
   Size:       6265
 
AclI AA'CG_TT

Cuts at:   1563    4392    4765    1563 
   Size:       2829     373    3063

  Fragments arranged by size: 

                3063    2829     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2844    4328    5712    5980    1632 
   Size:       1212    1484    1384     268    1917

  Fragments arranged by size: 

                1917    1484    1384    1212     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    6068
 
AflIII A'CryG_T

Cuts at:   1203    5164    6141    1203 
   Size:       3961     977    1327

  Fragments arranged by size: 

                3961    1327     977
 
AgeI A'CCGG_T

Cuts at:   5096    5096 
   Size:       6265
 
AhdI GACnn_n'nnGTC

Cuts at:   4994    4994 
   Size:       6265
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       6265
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       6265
 
AlwNI CAG_nnn'CTG

Cuts at:   2309    4008    5580    2309 
   Size:       1699    1572    2994

  Fragments arranged by size: 

                2994    1699    1572
 
ApaLI G'TGCA_C

Cuts at:   4322    5478    4322 
   Size:       1156    5109
 
ApoI r'AATT_y

Cuts at:   1493    3574    3800    3952    6061    1493 
   Size:       2081     226     152    2109    1697

  Fragments arranged by size: 

                2109    2081    1697     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    2400    4819     160 
   Size:        872    1368    2419    1606

  Fragments arranged by size: 

                2419    1606    1368     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6265
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3590    1870 
   Size:         73    1647    4545

  Fragments arranged by size: 

                4545    1647      73
 
BamHI G'GATC_C

Cuts at:   3595    3962    3595 
   Size:        367    5898
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3586    5041     618 
   Size:        325     217     627    1799    1455    1842

  Fragments arranged by size: 

                1842    1799    1455     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2875    3584    1391 
   Size:       1484     709    4072

  Fragments arranged by size: 

                4072    1484     709
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    3402     928 
   Size:        971    1503    3791

  Fragments arranged by size: 

                3791    1503     971
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4456    4490    1903 
   Size:         34    2519      34    3678

  Fragments arranged by size: 

                3678    2519      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2535    4157    5367    2535 
   Size:       1622    1210    3433

  Fragments arranged by size: 

                3433    1622    1210
 
BclI T'GATC_A

Cuts at:   1548    3250    1548 
   Size:       1702    4563
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2009    4876     136 
   Size:        107     122      71     631     605     337    2867    1525

  Fragments arranged by size: 

                2867    1525     631     605     337     122     107      71
            
 
BglII A'GATC_T

Cuts at:   2813    6260    2813 
   Size:       3447    2818
 
BlpI GC'TnA_GC

Cuts at:   2042    3682    2042 
   Size:       1640    4625
 
Bme1580I G_kGCm'C

Cuts at:   1790    4326    5482    1790 
   Size:       2536    1156    2573

  Fragments arranged by size: 

                2573    2536    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       6265
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2697    3996    4954 
   Size:        416      53      83     399      24    1280    1299     958

Cuts at:   4954     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1299    1280     958     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       6265
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2429    2429 
   Size:       6265
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2850    4925    1239 
   Size:       1611    2075    2579

  Fragments arranged by size: 

                2579    2075    1611
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   4390    5255    5553    4390 
   Size:        865     298    5102

  Fragments arranged by size: 

                5102     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2192    3189    4928     882 
   Size:        617     693     997    1739    2219

  Fragments arranged by size: 

                2219    1739     997     693     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3047    4056     493 
   Size:        711    1843    1009    2702

  Fragments arranged by size: 

                2702    1843    1009     711
 
BsaBI GATnn'nnATC

Cuts at:   6259    6259 
   Size:       6265
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3430    4072    4454    5089 
   Size:         53      83     186     936    1897     642     382     635

Cuts at:   5089     275 
   Size:       1451

  Fragments arranged by size: 

                1897    1451     936     642     635     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   2054    4698    5096    5370    5517    5928    2054 
   Size:       2644     398     274     147     411    2391

  Fragments arranged by size: 

                2644    2391     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2060    2090    2284    2314    2653 
   Size:         30     165      30     657      30     194      30     339

Cuts at:   2653    2683    1178 
   Size:         30    4760

  Fragments arranged by size: 

                4760     657     339     194     165      30      30      30
                  30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2081    2658    2865    2886    2904    2955    1186 
   Size:        895     577     207      21      18      51    4496

  Fragments arranged by size: 

                4496     895     577     207      51      21      18
 
BseYI C'CCAG_C

Cuts at:   5468    5964    5468 
   Size:        496    5769
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    3027    1163 
   Size:        573    1291    4401

  Fragments arranged by size: 

                4401    1291     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2013    3715    4476    5504    1540 
   Size:        275     186      12    1702     761    1028    2301

  Fragments arranged by size: 

                2301    1702    1028     761     275     186      12
 
BsiHKAI G_wGCw'C

Cuts at:   2281    2875    3545    4326    4411    5482    2281 
   Size:        594     670     781      85    1071    3064

  Fragments arranged by size: 

                3064    1071     781     670     594      85
 
BsmI GAATG_Cn'

Cuts at:   3776    3869    3776 
   Size:         93    6172
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2281    2875    3545    3584    4326    4411    5482 
   Size:        399     491     594     670      39     742      85    1071

Cuts at:   5482    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1071     742     670     594     491     399      85
                  39
 
BspEI T'CCGG_A

Cuts at:   5928    5928 
   Size:       6265
 
BspHI T'CATG_A

Cuts at:   4154    5884    4154 
   Size:       1730    4535
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3565    3620     844 
   Size:       2721      55    3489

  Fragments arranged by size: 

                3489    2721      55
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2046    4152    1394 
   Size:        429     223    2106    3507

  Fragments arranged by size: 

                3507    2106     429     223
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3070    4760    4934      65 
   Size:       1183    1822    1690     174    1396

  Fragments arranged by size: 

                1822    1690    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2012    4909    5096    1700 
   Size:        110     202    2897     187    2869

  Fragments arranged by size: 

                2897    2869     202     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       6265
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       6265
 
BssSI C'ACGA_G

Cuts at:   4325    5337    4325 
   Size:       1012    5253
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       6265
 
BstBI TT'CG_AA

Cuts at:   5069    5069 
   Size:       6265
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       6265
 
BstXI CCAn_nnnn'nTGG

Cuts at:   3086    3086 
   Size:       6265
 
BstYI r'GATC_y

Cuts at:   1972    2813    3595    3962    4347    4364    5816    5902    5914 
   Size:        841     782     367     385      17    1452      86      12

Cuts at:   5914    6260    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     841     782     385     367     346      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2450     513 
   Size:        552    1385    4328

  Fragments arranged by size: 

                4328    1385     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2103    2241    3297     487 
   Size:        683      56     877     138    1056    3455

  Fragments arranged by size: 

                3455    1056     877     683     138      56
 
BtsI GCAGTG_nn'

Cuts at:   2928    3137    3523    3781    4575    4595    2928 
   Size:        209     386     258     794      20    4598

  Fragments arranged by size: 

                4598     794     386     258     209      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       6265
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    6230
 
DraI TTT'AAA

Cuts at:   3569    3569 
   Size:       6265
 
DraIII CAC_nnn'GTG

Cuts at:   3269    3269 
   Size:       6265
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    5272     809 
   Size:        507    3956    1802

  Fragments arranged by size: 

                3956    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2010    3712    4601 
   Size:         54    1425      50     275      18     180    1702     889

Cuts at:   4601    6230       8 
   Size:       1629      43

  Fragments arranged by size: 

                1702    1629    1425     889     275     180      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2010    3712    1537 
   Size:        275     198    1702    4090

  Fragments arranged by size: 

                4090    1702     275     198
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2067    2332    3145    4195    1353 
   Size:        714     265     813    1050    3423

  Fragments arranged by size: 

                3423    1050     813     714     265
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4852    5226    5372     387 
   Size:       1064     138    3263     374     146    1280

  Fragments arranged by size: 

                3263    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2873    2873 
   Size:       6265
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2844    2850    4328    4925    5712    5980    1239 
   Size:        393    1212       6    1478     597     787     268    1524

  Fragments arranged by size: 

                1524    1478    1212     787     597     393     268       6
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2970    2970 
   Size:       6265
 
EcoO109I rG'GnC_Cy

Cuts at:   2800    2800 
   Size:       6265
 
EcoRI G'AATT_C

Cuts at:   3574    3952    3574 
   Size:        378    5887
 
FspI TGC'GCA

Cuts at:   4771    4771 
   Size:       6265
 
HaeII r_GCGC'y

Cuts at:   1678    2029    2979    5412    1678 
   Size:        351     950    2433    2531

  Fragments arranged by size: 

                2531    2433     950     351
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    4906    4938    4980    5012    1373 
   Size:         32     135      32    3334      32      42      32    2626

  Fragments arranged by size: 

                3334    2626     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3609    3855     669 
   Size:        988     281    1671     246    3079

  Fragments arranged by size: 

                3079    1671     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2517     748 
   Size:       1769    4496
 
HpaI GTT'AAC

Cuts at:   3855    3855 
   Size:       6265
 
KpnI G_GTAC'C

Cuts at:   3590    3590 
   Size:       6265
 
MfeI C'AATT_G

Cuts at:   2721    3864    2721 
   Size:       1143    5122
 
MluI A'CGCG_T

Cuts at:   6141    6141 
   Size:       6265
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2016    2942    5379    5563    1305 
   Size:        711     926    2437     184    2007

  Fragments arranged by size: 

                2437    2007     926     711     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    4443

  Fragments arranged by size: 

                4443    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3441    4223    4582    4741     518 
   Size:        648    2275     782     359     159    2042

  Fragments arranged by size: 

                2275    2042     782     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    3681    4358    5506    5751    1606 
   Size:       2075     677    1148     245    2120

  Fragments arranged by size: 

                2120    2075    1148     677     245
 
NaeI GCC'GGC

Cuts at:   2014    2014 
   Size:       6265
 
NcoI C'CATG_G

Cuts at:    513    1065    2450     513 
   Size:        552    1385    4328

  Fragments arranged by size: 

                4328    1385     552
 
NdeI CA'TA_TG

Cuts at:    387    3058     387 
   Size:       2671    3594
 
NgoMIV G'CCGG_C

Cuts at:   2012    2012 
   Size:       6265
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       6265
 
NotI GC'GGCC_GC

Cuts at:   3712    3712 
   Size:       6265
 
NspI r_CATG'y

Cuts at:   1132    1229    3623    5168    1132 
   Size:         97    2394    1545    2229

  Fragments arranged by size: 

                2394    2229    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       6265
 
PciI A'CATG_T

Cuts at:   5164    5164 
   Size:       6265
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2288    1108 
   Size:       1180    5085
 
PfoI T'CCnGG_A

Cuts at:   2866    2866 
   Size:       6265
 
PmeI GTTT'AAAC

Cuts at:   3569    3569 
   Size:       6265
 
PmlI CAC'GTG

Cuts at:   3047    3047 
   Size:       6265
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4334    4366    5847    5879    4334 
   Size:         32    1481      32    4720

  Fragments arranged by size: 

                4720    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2800    2800 
   Size:       6265
 
PshAI GACnn'nnGTC

Cuts at:   1613    2363    3406    1613 
   Size:        750    1043    4472

  Fragments arranged by size: 

                4472    1043     750
 
PsiI TTA'TAA

Cuts at:   3835    3835 
   Size:       6265
 
PstI C_TGCA'G

Cuts at:    830    3562    3617     830 
   Size:       2732      55    3478

  Fragments arranged by size: 

                3478    2732      55
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       6265
 
SacI G_AGCT'C

Cuts at:   2875    2875 
   Size:       6265
 
SalI G'TCGA_C

Cuts at:   1936    3607    1936 
   Size:       1671    4594
 
SbfI CC_TGCA'GG

Cuts at:   3617    3617 
   Size:       6265
 
ScaI AGT'ACT

Cuts at:   4513    6225    4513 
   Size:       1712    4553
 
SexAI A'CCwGG_T

Cuts at:   2461    2461 
   Size:       6265
 
SfcI C'TryA_G

Cuts at:    826    1045    2256    2352    3558    3613    4748    5429    5620 
   Size:        219    1211      96    1206      55    1135     681     191

Cuts at:   5620     826 
   Size:       1471

  Fragments arranged by size: 

                1471    1211    1206    1135     681     219     191      96
                  55
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6265
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       6265
 
SmaI CCC'GGG

Cuts at:   1872    3592    1872 
   Size:       1720    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    4369    5270    5532     820 
   Size:        197     926    2426     901     262    1553

  Fragments arranged by size: 

                2426    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    4056     493 
   Size:       3563    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       6265
 
SphI G_CATG'C

Cuts at:   1132    3623    1132 
   Size:       2491    3774
 
SspI AAT'ATT

Cuts at:      5      52    4189       5 
   Size:         47    4137    2081

  Fragments arranged by size: 

                4137    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       6265
 
StyI C'CwwG_G

Cuts at:    513    1065    2450    3704     513 
   Size:        552    1385    1254    3074

  Fragments arranged by size: 

                3074    1385    1254     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2216    2723    3177    4292    4309    4462    4647 
   Size:       1255     636     507     454    1115      17     153     185

Cuts at:   4647    5092     325 
   Size:        445    1498

  Fragments arranged by size: 

                1498    1255    1115     636     507     454     445     185
                 153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    4511    6223      96 
   Size:        274      80      33      51    1443    2534    1712     138

  Fragments arranged by size: 

                2534    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2060    2092    2872    2904    6225    6257    2060 
   Size:         32     780      32    3321      32    2068

  Fragments arranged by size: 

                3321    2068     780      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       6265
 
XbaI T'CTAG_A

Cuts at:   3601    3601 
   Size:       6265
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       6265
 
XmaI C'CCGG_G

Cuts at:   1870    3590    1870 
   Size:       1720    4545
 
XmnI GAAnn'nnTTC

Cuts at:   4394    4394 
   Size:       6265
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3431    4073     276 
   Size:         53      83     186     936    1897     642    2468

  Fragments arranged by size: 

                2468    1897     936     642     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FspI
    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NcoI     NdeI   NgoMIV
     NheI     NotI     NspI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI     PstI     PvuI     SacI
     SalI     SbfI     ScaI    SexAI     SfcI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI   Bpu10I    BsiWI    BsmBI  BstZ17I   Bsu36I    EcoRV     FalI
     FseI    FspAI     KasI     NarI     NruI     NsiI     PacI   PspOMI
    PspXI     PsrI    PvuII    RsrII    SacII    SanDI     SapI     SfiI
     SfoI     SrfI     SwaI     XcmI
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