(Circular) (Six-base) MAPSORT of: pFN21AB8603.seq Check: 2961 from: 1 to: 5941 pFN21AB8603 5941 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3751 278 Size: 53 83 186 936 2215 2468 Fragments arranged by size: 2468 2215 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3284 1937 Size: 1347 4594 Acc65I G'GTAC_C Cuts at: 2115 3262 2115 Size: 1147 4794 AclI AA'CG_TT Cuts at: 1563 4068 4441 1563 Size: 2505 373 3063 Fragments arranged by size: 3063 2505 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2798 4004 5388 5656 1632 Size: 1166 1206 1384 268 1917 Fragments arranged by size: 1917 1384 1206 1166 268 AfeI AGC'GCT Cuts at: 3119 3119 Size: 5941 AflII C'TTAA_G Cuts at: 820 1017 2294 820 Size: 197 1277 4467 Fragments arranged by size: 4467 1277 197 AflIII A'CryG_T Cuts at: 1203 4840 5817 1203 Size: 3637 977 1327 Fragments arranged by size: 3637 1327 977 AgeI A'CCGG_T Cuts at: 4772 4772 Size: 5941 AhdI GACnn_n'nnGTC Cuts at: 4670 4670 Size: 5941 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5941 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5941 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3006 3038 3006 Size: 32 5909 AlwNI CAG_nnn'CTG Cuts at: 3194 3684 5256 3194 Size: 490 1572 3879 Fragments arranged by size: 3879 1572 490 ApaLI G'TGCA_C Cuts at: 3998 5154 3998 Size: 1156 4785 ApoI r'AATT_y Cuts at: 1493 2009 2201 3250 3476 3628 5737 1493 Size: 516 192 1049 226 152 2109 1697 Fragments arranged by size: 2109 1697 1049 516 226 192 152 AseI AT'TA_AT Cuts at: 160 1032 4495 160 Size: 872 3463 1606 Fragments arranged by size: 3463 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 AvaI C'yCGr_G Cuts at: 1870 1943 2937 3266 1870 Size: 73 994 329 4545 Fragments arranged by size: 4545 994 329 73 BamHI G'GATC_C Cuts at: 2555 3271 3638 2555 Size: 716 367 4858 Fragments arranged by size: 4858 716 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2115 2980 3262 4717 618 Size: 325 217 627 328 865 282 1455 1842 Fragments arranged by size: 1842 1455 865 627 328 325 282 217 BanII G_rGCy'C Cuts at: 1391 2931 3260 1391 Size: 1540 329 4072 Fragments arranged by size: 4072 1540 329 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2883 928 Size: 971 984 3986 Fragments arranged by size: 3986 984 971 BbvCI CC'TCA_GC Cuts at: 2719 2719 Size: 5941 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4132 4166 1903 Size: 34 2195 34 3678 Fragments arranged by size: 3678 2195 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3833 5043 3833 Size: 1210 4731 BclI T'GATC_A Cuts at: 1548 1548 Size: 5941 BfrBI ATG'CAT Cuts at: 2381 2381 Size: 5941 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4552 136 Size: 107 122 71 631 605 2880 1525 Fragments arranged by size: 2880 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5936 5936 Size: 5941 BlpI GC'TnA_GC Cuts at: 2315 3358 2315 Size: 1043 4898 Bme1580I G_kGCm'C Cuts at: 1790 2985 4002 5158 1790 Size: 1195 1017 1156 2573 Fragments arranged by size: 2573 1195 1156 1017 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5941 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2960 3672 4630 Size: 416 53 83 399 24 1543 712 958 Cuts at: 4630 442 Size: 1753 Fragments arranged by size: 1753 1543 958 712 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5941 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4601 1239 Size: 3362 2579 Bpu10I CC'TnA_GC Cuts at: 2719 2719 Size: 5941 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2153 3250 4066 4931 5229 2153 Size: 1097 816 865 298 2865 Fragments arranged by size: 2865 1097 865 816 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4604 882 Size: 617 3105 2219 Fragments arranged by size: 3105 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3732 493 Size: 711 2528 2702 Fragments arranged by size: 2702 2528 711 BsaBI GATnn'nnATC Cuts at: 5935 5935 Size: 5941 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3748 4130 4765 275 Size: 53 83 186 936 2215 382 635 1451 Fragments arranged by size: 2215 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2676 4374 4772 5046 5193 5604 2676 Size: 1698 398 274 147 411 3013 Fragments arranged by size: 3013 1698 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2765 2795 3191 3221 1178 Size: 30 165 30 1362 30 396 30 3898 Fragments arranged by size: 3898 1362 396 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2768 2877 1186 Size: 1582 109 4250 Fragments arranged by size: 4250 1582 109 BseYI C'CCAG_C Cuts at: 5144 5640 5144 Size: 496 5445 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2217 1163 Size: 573 481 4887 Fragments arranged by size: 4887 573 481 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3391 4152 5180 1540 Size: 275 186 1390 761 1028 2301 Fragments arranged by size: 2301 1390 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2948 4002 4087 5158 2948 Size: 1054 85 1071 3731 Fragments arranged by size: 3731 1071 1054 85 BsmI GAATG_Cn' Cuts at: 2070 2857 3452 3545 2070 Size: 787 595 93 4466 Fragments arranged by size: 4466 787 595 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2931 2948 2985 3260 4002 4087 5158 Size: 399 1141 17 37 275 742 85 1071 Cuts at: 5158 1391 Size: 2174 Fragments arranged by size: 2174 1141 1071 742 399 275 85 37 17 BspEI T'CCGG_A Cuts at: 2676 5604 2676 Size: 2928 3013 BspHI T'CATG_A Cuts at: 2916 3180 3830 5560 2916 Size: 264 650 1730 3297 Fragments arranged by size: 3297 1730 650 264 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2136 3296 844 Size: 1292 1160 3489 Fragments arranged by size: 3489 1292 1160 BsrBI CCG'CTC Cuts at: 1394 1823 3828 1394 Size: 429 2005 3507 Fragments arranged by size: 3507 2005 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4436 4610 65 Size: 1183 3188 174 1396 Fragments arranged by size: 3188 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4585 4772 1700 Size: 110 2775 187 2869 Fragments arranged by size: 2869 2775 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5941 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5941 BssSI C'ACGA_G Cuts at: 4001 5013 4001 Size: 1012 4929 BstAPI GCAn_nnn'nTGC Cuts at: 794 2387 794 Size: 1593 4348 BstBI TT'CG_AA Cuts at: 4745 4745 Size: 5941 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5941 BstYI r'GATC_y Cuts at: 1972 2042 2555 2758 3271 3638 4023 4040 5492 Size: 70 513 203 513 367 385 17 1452 Cuts at: 5492 5578 5590 5936 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 513 513 385 367 346 203 86 70 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4450 Fragments arranged by size: 4450 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5202 Fragments arranged by size: 5202 683 56 BtsI GCAGTG_nn' Cuts at: 2463 3457 4251 4271 2463 Size: 994 794 20 4133 Fragments arranged by size: 4133 994 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5941 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5906 DraI TTT'AAA Cuts at: 3245 3245 Size: 5941 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4948 809 Size: 507 3632 1802 Fragments arranged by size: 3632 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3388 4277 5906 Size: 54 1425 50 275 18 1558 889 1629 Cuts at: 5906 8 Size: 43 Fragments arranged by size: 1629 1558 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3388 1537 Size: 275 1576 4090 Fragments arranged by size: 4090 1576 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2513 2532 3871 1353 Size: 1160 19 1339 3423 Fragments arranged by size: 3423 1339 1160 19 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4528 4902 5048 387 Size: 1064 138 2939 374 146 1280 Fragments arranged by size: 2939 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2798 4004 4601 5388 5656 1239 Size: 393 1166 1206 597 787 268 1524 Fragments arranged by size: 1524 1206 1166 787 597 393 268 EcoRI G'AATT_C Cuts at: 2201 3250 3628 2201 Size: 1049 378 4514 Fragments arranged by size: 4514 1049 378 EcoRV GAT'ATC Cuts at: 2791 2791 Size: 5941 FspI TGC'GCA Cuts at: 4447 4447 Size: 5941 HaeII r_GCGC'y Cuts at: 1678 3121 5088 1678 Size: 1443 1967 2531 Fragments arranged by size: 2531 1967 1443 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2308 2340 2765 2797 3092 Size: 32 135 32 736 32 425 32 295 Cuts at: 3092 3124 4582 4614 4656 4688 1373 Size: 32 1458 32 42 32 2626 Fragments arranged by size: 2626 1458 736 425 295 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3067 3285 3531 669 Size: 988 281 1129 218 246 3079 Fragments arranged by size: 3079 1129 988 281 246 218 HindIII A'AGCT_T Cuts at: 748 748 Size: 5941 HpaI GTT'AAC Cuts at: 3531 3531 Size: 5941 KpnI G_GTAC'C Cuts at: 2119 3266 2119 Size: 1147 4794 MfeI C'AATT_G Cuts at: 3169 3540 3169 Size: 371 5570 MluI A'CGCG_T Cuts at: 5817 5817 Size: 5941 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2122 3040 5055 5239 1305 Size: 817 918 2015 184 2007 Fragments arranged by size: 2015 2007 918 817 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4119 Fragments arranged by size: 4119 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2480 3899 4258 4417 518 Size: 648 1314 1419 359 159 2042 Fragments arranged by size: 2042 1419 1314 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3357 4034 5182 5427 1606 Size: 1751 677 1148 245 2120 Fragments arranged by size: 2120 1751 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4450 Fragments arranged by size: 4450 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5941 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5941 NotI GC'GGCC_GC Cuts at: 3388 3388 Size: 5941 NsiI A_TGCA'T Cuts at: 2383 2383 Size: 5941 NspI r_CATG'y Cuts at: 1132 1229 3299 4844 1132 Size: 97 2070 1545 2229 Fragments arranged by size: 2229 2070 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5941 PciI A'CATG_T Cuts at: 4840 4840 Size: 5941 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5941 PmeI GTTT'AAAC Cuts at: 3245 3245 Size: 5941 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4010 4042 5523 5555 4010 Size: 32 1481 32 4396 Fragments arranged by size: 4396 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5941 PsiI TTA'TAA Cuts at: 2804 3511 2804 Size: 707 5234 PstI C_TGCA'G Cuts at: 830 2266 3293 830 Size: 1436 1027 3478 Fragments arranged by size: 3478 1436 1027 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5941 SalI G'TCGA_C Cuts at: 1936 3283 1936 Size: 1347 4594 SapI GCTCTTCn'nnn_ Cuts at: 2513 2532 2513 Size: 19 5922 SbfI CC_TGCA'GG Cuts at: 3293 3293 Size: 5941 ScaI AGT'ACT Cuts at: 4189 5901 4189 Size: 1712 4229 SexAI A'CCwGG_T Cuts at: 3054 3054 Size: 5941 SfcI C'TryA_G Cuts at: 826 1045 2262 2564 2794 3289 4424 5105 5296 Size: 219 1217 302 230 495 1135 681 191 Cuts at: 5296 826 Size: 1471 Fragments arranged by size: 1471 1217 1135 681 495 302 230 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5941 SmaI CCC'GGG Cuts at: 1872 3268 1872 Size: 1396 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2132 2294 2937 3229 4045 4946 Size: 197 926 189 162 643 292 816 901 Cuts at: 4946 5208 820 Size: 262 1553 Fragments arranged by size: 1553 926 901 816 643 292 262 197 189 162 SnaBI TAC'GTA Cuts at: 493 3732 493 Size: 3239 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5941 SphI G_CATG'C Cuts at: 1132 3299 1132 Size: 2167 3774 SspI AAT'ATT Cuts at: 5 52 3865 5 Size: 47 3813 2081 Fragments arranged by size: 3813 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5941 StyI C'CwwG_G Cuts at: 513 1065 2004 3380 513 Size: 552 939 1376 3074 Fragments arranged by size: 3074 1376 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3968 3985 4138 4323 4768 325 Size: 1255 2388 17 153 185 445 1498 Fragments arranged by size: 2388 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4187 5899 96 Size: 274 80 33 51 1443 2210 1712 138 Fragments arranged by size: 2210 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5901 5933 5901 Size: 32 5909 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5941 XbaI T'CTAG_A Cuts at: 3277 3277 Size: 5941 XhoI C'TCGA_G Cuts at: 1943 2937 1943 Size: 994 4947 XmaI C'CCGG_G Cuts at: 1870 3266 1870 Size: 1396 4545 XmnI GAAnn'nnTTC Cuts at: 2427 2688 4070 2427 Size: 261 1382 4298 Fragments arranged by size: 4298 1382 261 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3749 276 Size: 53 83 186 936 2215 2468 Fragments arranged by size: 2468 2215 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI