Restriction map for pFN21AB8603
 (Circular) (Six-base) MAPSORT of: pFN21AB8603.seq  Check: 2961  from: 1  to: 5941



pFN21AB8603  5941 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:27  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3751     278 
   Size:         53      83     186     936    2215    2468

  Fragments arranged by size: 

                2468    2215     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3284    1937 
   Size:       1347    4594
 
Acc65I G'GTAC_C

Cuts at:   2115    3262    2115 
   Size:       1147    4794
 
AclI AA'CG_TT

Cuts at:   1563    4068    4441    1563 
   Size:       2505     373    3063

  Fragments arranged by size: 

                3063    2505     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2798    4004    5388    5656    1632 
   Size:       1166    1206    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1206    1166     268
 
AfeI AGC'GCT

Cuts at:   3119    3119 
   Size:       5941
 
AflII C'TTAA_G

Cuts at:    820    1017    2294     820 
   Size:        197    1277    4467

  Fragments arranged by size: 

                4467    1277     197
 
AflIII A'CryG_T

Cuts at:   1203    4840    5817    1203 
   Size:       3637     977    1327

  Fragments arranged by size: 

                3637    1327     977
 
AgeI A'CCGG_T

Cuts at:   4772    4772 
   Size:       5941
 
AhdI GACnn_n'nnGTC

Cuts at:   4670    4670 
   Size:       5941
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5941
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5941
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3006    3038    3006 
   Size:         32    5909
 
AlwNI CAG_nnn'CTG

Cuts at:   3194    3684    5256    3194 
   Size:        490    1572    3879

  Fragments arranged by size: 

                3879    1572     490
 
ApaLI G'TGCA_C

Cuts at:   3998    5154    3998 
   Size:       1156    4785
 
ApoI r'AATT_y

Cuts at:   1493    2009    2201    3250    3476    3628    5737    1493 
   Size:        516     192    1049     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1049     516     226     192     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4495     160 
   Size:        872    3463    1606

  Fragments arranged by size: 

                3463    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5941
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2937    3266    1870 
   Size:         73     994     329    4545

  Fragments arranged by size: 

                4545     994     329      73
 
BamHI G'GATC_C

Cuts at:   2555    3271    3638    2555 
   Size:        716     367    4858

  Fragments arranged by size: 

                4858     716     367
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2115    2980    3262    4717     618 
   Size:        325     217     627     328     865     282    1455    1842

  Fragments arranged by size: 

                1842    1455     865     627     328     325     282     217
            
 
BanII G_rGCy'C

Cuts at:   1391    2931    3260    1391 
   Size:       1540     329    4072

  Fragments arranged by size: 

                4072    1540     329
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2883     928 
   Size:        971     984    3986

  Fragments arranged by size: 

                3986     984     971
 
BbvCI CC'TCA_GC

Cuts at:   2719    2719 
   Size:       5941
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4132    4166    1903 
   Size:         34    2195      34    3678

  Fragments arranged by size: 

                3678    2195      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3833    5043    3833 
   Size:       1210    4731
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5941
 
BfrBI ATG'CAT

Cuts at:   2381    2381 
   Size:       5941
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4552     136 
   Size:        107     122      71     631     605    2880    1525

  Fragments arranged by size: 

                2880    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5936    5936 
   Size:       5941
 
BlpI GC'TnA_GC

Cuts at:   2315    3358    2315 
   Size:       1043    4898
 
Bme1580I G_kGCm'C

Cuts at:   1790    2985    4002    5158    1790 
   Size:       1195    1017    1156    2573

  Fragments arranged by size: 

                2573    1195    1156    1017
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5941
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2960    3672    4630 
   Size:        416      53      83     399      24    1543     712     958

Cuts at:   4630     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1543     958     712     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5941
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    4601    1239 
   Size:       3362    2579
 
Bpu10I CC'TnA_GC

Cuts at:   2719    2719 
   Size:       5941
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2153    3250    4066    4931    5229    2153 
   Size:       1097     816     865     298    2865

  Fragments arranged by size: 

                2865    1097     865     816     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4604     882 
   Size:        617    3105    2219

  Fragments arranged by size: 

                3105    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3732     493 
   Size:        711    2528    2702

  Fragments arranged by size: 

                2702    2528     711
 
BsaBI GATnn'nnATC

Cuts at:   5935    5935 
   Size:       5941
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3748    4130    4765     275 
   Size:         53      83     186     936    2215     382     635    1451

  Fragments arranged by size: 

                2215    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   2676    4374    4772    5046    5193    5604    2676 
   Size:       1698     398     274     147     411    3013

  Fragments arranged by size: 

                3013    1698     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2765    2795    3191    3221    1178 
   Size:         30     165      30    1362      30     396      30    3898

  Fragments arranged by size: 

                3898    1362     396     165      30      30      30      30
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2768    2877    1186 
   Size:       1582     109    4250

  Fragments arranged by size: 

                4250    1582     109
 
BseYI C'CCAG_C

Cuts at:   5144    5640    5144 
   Size:        496    5445
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2217    1163 
   Size:        573     481    4887

  Fragments arranged by size: 

                4887     573     481
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3391    4152    5180    1540 
   Size:        275     186    1390     761    1028    2301

  Fragments arranged by size: 

                2301    1390    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2948    4002    4087    5158    2948 
   Size:       1054      85    1071    3731

  Fragments arranged by size: 

                3731    1071    1054      85
 
BsmI GAATG_Cn'

Cuts at:   2070    2857    3452    3545    2070 
   Size:        787     595      93    4466

  Fragments arranged by size: 

                4466     787     595      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2931    2948    2985    3260    4002    4087    5158 
   Size:        399    1141      17      37     275     742      85    1071

Cuts at:   5158    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1141    1071     742     399     275      85      37
                  17
 
BspEI T'CCGG_A

Cuts at:   2676    5604    2676 
   Size:       2928    3013
 
BspHI T'CATG_A

Cuts at:   2916    3180    3830    5560    2916 
   Size:        264     650    1730    3297

  Fragments arranged by size: 

                3297    1730     650     264
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2136    3296     844 
   Size:       1292    1160    3489

  Fragments arranged by size: 

                3489    1292    1160
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3828    1394 
   Size:        429    2005    3507

  Fragments arranged by size: 

                3507    2005     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4436    4610      65 
   Size:       1183    3188     174    1396

  Fragments arranged by size: 

                3188    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4585    4772    1700 
   Size:        110    2775     187    2869

  Fragments arranged by size: 

                2869    2775     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5941
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5941
 
BssSI C'ACGA_G

Cuts at:   4001    5013    4001 
   Size:       1012    4929
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2387     794 
   Size:       1593    4348
 
BstBI TT'CG_AA

Cuts at:   4745    4745 
   Size:       5941
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5941
 
BstYI r'GATC_y

Cuts at:   1972    2042    2555    2758    3271    3638    4023    4040    5492 
   Size:         70     513     203     513     367     385      17    1452

Cuts at:   5492    5578    5590    5936    1972 
   Size:         86      12     346    1977

  Fragments arranged by size: 

                1977    1452     513     513     385     367     346     203
                  86      70      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4450

  Fragments arranged by size: 

                4450     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5202

  Fragments arranged by size: 

                5202     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2463    3457    4251    4271    2463 
   Size:        994     794      20    4133

  Fragments arranged by size: 

                4133     994     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5941
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5906
 
DraI TTT'AAA

Cuts at:   3245    3245 
   Size:       5941
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4948     809 
   Size:        507    3632    1802

  Fragments arranged by size: 

                3632    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    3388    4277    5906 
   Size:         54    1425      50     275      18    1558     889    1629

Cuts at:   5906       8 
   Size:         43

  Fragments arranged by size: 

                1629    1558    1425     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3388    1537 
   Size:        275    1576    4090

  Fragments arranged by size: 

                4090    1576     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2513    2532    3871    1353 
   Size:       1160      19    1339    3423

  Fragments arranged by size: 

                3423    1339    1160      19
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4528    4902    5048     387 
   Size:       1064     138    2939     374     146    1280

  Fragments arranged by size: 

                2939    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2798    4004    4601    5388    5656    1239 
   Size:        393    1166    1206     597     787     268    1524

  Fragments arranged by size: 

                1524    1206    1166     787     597     393     268
 
EcoRI G'AATT_C

Cuts at:   2201    3250    3628    2201 
   Size:       1049     378    4514

  Fragments arranged by size: 

                4514    1049     378
 
EcoRV GAT'ATC

Cuts at:   2791    2791 
   Size:       5941
 
FspI TGC'GCA

Cuts at:   4447    4447 
   Size:       5941
 
HaeII r_GCGC'y

Cuts at:   1678    3121    5088    1678 
   Size:       1443    1967    2531

  Fragments arranged by size: 

                2531    1967    1443
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2308    2340    2765    2797    3092 
   Size:         32     135      32     736      32     425      32     295

Cuts at:   3092    3124    4582    4614    4656    4688    1373 
   Size:         32    1458      32      42      32    2626

  Fragments arranged by size: 

                2626    1458     736     425     295     135      42      32
                  32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3067    3285    3531     669 
   Size:        988     281    1129     218     246    3079

  Fragments arranged by size: 

                3079    1129     988     281     246     218
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5941
 
HpaI GTT'AAC

Cuts at:   3531    3531 
   Size:       5941
 
KpnI G_GTAC'C

Cuts at:   2119    3266    2119 
   Size:       1147    4794
 
MfeI C'AATT_G

Cuts at:   3169    3540    3169 
   Size:        371    5570
 
MluI A'CGCG_T

Cuts at:   5817    5817 
   Size:       5941
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2122    3040    5055    5239    1305 
   Size:        817     918    2015     184    2007

  Fragments arranged by size: 

                2015    2007     918     817     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    4119

  Fragments arranged by size: 

                4119    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2480    3899    4258    4417     518 
   Size:        648    1314    1419     359     159    2042

  Fragments arranged by size: 

                2042    1419    1314     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    3357    4034    5182    5427    1606 
   Size:       1751     677    1148     245    2120

  Fragments arranged by size: 

                2120    1751    1148     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4450

  Fragments arranged by size: 

                4450     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5941
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5941
 
NotI GC'GGCC_GC

Cuts at:   3388    3388 
   Size:       5941
 
NsiI A_TGCA'T

Cuts at:   2383    2383 
   Size:       5941
 
NspI r_CATG'y

Cuts at:   1132    1229    3299    4844    1132 
   Size:         97    2070    1545    2229

  Fragments arranged by size: 

                2229    2070    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5941
 
PciI A'CATG_T

Cuts at:   4840    4840 
   Size:       5941
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5941
 
PmeI GTTT'AAAC

Cuts at:   3245    3245 
   Size:       5941
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4010    4042    5523    5555    4010 
   Size:         32    1481      32    4396

  Fragments arranged by size: 

                4396    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5941
 
PsiI TTA'TAA

Cuts at:   2804    3511    2804 
   Size:        707    5234
 
PstI C_TGCA'G

Cuts at:    830    2266    3293     830 
   Size:       1436    1027    3478

  Fragments arranged by size: 

                3478    1436    1027
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5941
 
SalI G'TCGA_C

Cuts at:   1936    3283    1936 
   Size:       1347    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2513    2532    2513 
   Size:         19    5922
 
SbfI CC_TGCA'GG

Cuts at:   3293    3293 
   Size:       5941
 
ScaI AGT'ACT

Cuts at:   4189    5901    4189 
   Size:       1712    4229
 
SexAI A'CCwGG_T

Cuts at:   3054    3054 
   Size:       5941
 
SfcI C'TryA_G

Cuts at:    826    1045    2262    2564    2794    3289    4424    5105    5296 
   Size:        219    1217     302     230     495    1135     681     191

Cuts at:   5296     826 
   Size:       1471

  Fragments arranged by size: 

                1471    1217    1135     681     495     302     230     219
                 191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5941
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5941
 
SmaI CCC'GGG

Cuts at:   1872    3268    1872 
   Size:       1396    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2132    2294    2937    3229    4045    4946 
   Size:        197     926     189     162     643     292     816     901

Cuts at:   4946    5208     820 
   Size:        262    1553

  Fragments arranged by size: 

                1553     926     901     816     643     292     262     197
                 189     162
 
SnaBI TAC'GTA

Cuts at:    493    3732     493 
   Size:       3239    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5941
 
SphI G_CATG'C

Cuts at:   1132    3299    1132 
   Size:       2167    3774
 
SspI AAT'ATT

Cuts at:      5      52    3865       5 
   Size:         47    3813    2081

  Fragments arranged by size: 

                3813    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5941
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    3380     513 
   Size:        552     939    1376    3074

  Fragments arranged by size: 

                3074    1376     939     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3968    3985    4138    4323    4768     325 
   Size:       1255    2388      17     153     185     445    1498

  Fragments arranged by size: 

                2388    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    4187    5899      96 
   Size:        274      80      33      51    1443    2210    1712     138

  Fragments arranged by size: 

                2210    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5901    5933    5901 
   Size:         32    5909
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5941
 
XbaI T'CTAG_A

Cuts at:   3277    3277 
   Size:       5941
 
XhoI C'TCGA_G

Cuts at:   1943    2937    1943 
   Size:        994    4947
 
XmaI C'CCGG_G

Cuts at:   1870    3266    1870 
   Size:       1396    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2427    2688    4070    2427 
   Size:        261    1382    4298

  Fragments arranged by size: 

                4298    1382     261
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3749     276 
   Size:         53      83     186     936    2215    2468

  Fragments arranged by size: 

                2468    2215     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI
     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI
     EagI     EarI     EciI  Eco57MI    EcoRI    EcoRV     FspI    HaeII
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NsiI
     NspI     PasI     PciI    PflMI     PmeI     PpiI    PshAI     PsiI
     PstI     PvuI     SalI     SapI     SbfI     ScaI    SexAI     SfcI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     ApaI     AscI    AvrII     BaeI     BbeI     BplI    BsiWI
    BsmBI    BstXI  BstZ17I   Bsu36I   DraIII  EcoICRI    EcoNI EcoO109I
     FalI     FseI    FspAI     KasI     NaeI     NarI   NgoMIV     NruI
     PacI     PfoI     PmlI    PpuMI   PspOMI    PspXI     PsrI    PvuII
    RsrII     SacI    SacII    SanDI     SfiI     SfoI     SrfI     SwaI
     XcmI
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