Restriction map for
pFN21AB8589
##################################
# NO CUT ENZYMES ( 149 enzymes) #
##################################
AccII AccIII AclI AflII AgeI
AhdI AlwI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I AspEI
AspI AvaII AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BcgI
BciVI BcnI BfrI BglI BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BseRI BsiWI
BsmBI BsmI Bsp1407I BspEI BspMI
BsrDI BsrFI BsrGI BssHII BssKI
BssSI Bst1107I BstBI BstEII BstNI
BstPI BstUI BstXI BstZ17I Bsu36I
CelII Cfr10I CpoI DraI DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRII EcoRV EcoT14I
FauI FseI FspI HaeII HapII
HgaI HindIII HpaII KasI Ksp632I
KspI MamI MfeI MluI MluNI
MroI MscI MspI MunI MvaI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I RsrII SacII
SapI SbfI ScaI ScrFI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspBI SspI
StyI SwaI Tth111I Van91I XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AatII 479 GACGTC GACGT^C
Acc65I 828 GGTACC G^GTACC
AccI 218 GTCGAC GT^[AC][GT]AC
AcsI 227 AAATTC [GA]^AATT[CT]
AcyI 479 GACGTC G[GA]^CG[CT]C
AflIII 124 ACATGT A^C[GA][CT]GT
Alw44I 1056 GTGCAC G^TGCAC
AlwNI 1061 CAGTAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1056 GTGCAC G^TGCAC
ApoI 227 AAATTC [GA]^AATT[CT]
Asp718I 828 GGTACC G^GTACC
AvaI 232 CCCGAG C^[CT]CG[GA]G
BanI 828 GGTACC G^G[CT][GA]CC
BanII 582 GAGCTC G[GA]GC[CT]^C
BbvI 283 GCAGC GCAGC(8/12)
BclI 21 TGATCA T^GATCA
BglII 1033 AGATCT A^GATCT
BsaHI 479 GACGTC G[GA]^CG[CT]C
BsaJI 233 CCGAGG C^C[ACGT][ACGT]GG
BsgI 80 CTGCAC GTGCAG(16/14)
BsmFI 97 GTCCC GGGAC(10/14)
BsoBI 232 CCCGAG C^[CT]CG[GA]G
BspHI 804 TCATGA T^CATGA
BspLU11I 124 ACATGT A^CATGT
BsrBI 596 GAGCGG CCGCTC(-3/-3)
BstAPI 898 GCAGTTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1033 AGATCT [GA]^GATC[CT]
CfoI 354 GCGC GCG^C
Cfr13I 656 GGCCC G^G[ACGT]CC
DraII 655 AGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 614 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 655 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT22I 308 ATGCAT ATGCA^T
FbaI 21 TGATCA T^GATCA
Fnu4HI 283 GCAGC GC^[ACGT]GC
HhaI 354 GCGC GCG^C
Hin1I 479 GACGTC G[GA]^CG[CT]C
HinP1I 354 GCGC G^CGC
HpaI 681 GTTAAC GTT^AAC
ItaI 283 GCAGC GC^[ACGT]GC
KpnI 828 GGTACC GGTAC^C
MflI 1033 AGATCT [GA]^GATC[CT]
MslI 402 CACTGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 767 CAGCTG C[AC]G^C[GT]G
NsiI 308 ATGCAT ATGCA^T
PstI 896 CTGCAG CTGCA^G
PvuII 767 CAGCTG CAG^CTG
RcaI 804 TCATGA T^CATGA
SacI 582 GAGCTC GAGCT^C
SalI 218 GTCGAC G^TCGAC
Sau96I 656 GGCCC G^G[ACGT]CC
SfcI 896 CTGCAG C^T[GA][CT]AG
SmlI 277 CTCAAG C^T[CT][GA]AG
SpeI 358 ACTAGT A^CTAGT
StuI 1042 AGGCCT AGG^CCT
TseI 283 GCAGC G^C[AT]GC
Tsp45I 264 GTCAC ^GT[GC]AC
XbaI 1036 TCTAGA T^CTAGA
XhoII 1033 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AciI 204 GCGG CCGC(-3/-1)
598 GCGG
BbsI 727 GAAGAC GAAGAC(2/6)
645 GTCTTC
BpuAI 727 GAAGAC GAAGAC(2/6)
645 GTCTTC
BsiEI 966 CGATCG CG[GA][CT]^CG
979 CGATCG
BsiYI 517 CCAAAGAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
814 CCATCTTCAGG
BslI 517 CCAAAGAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
814 CCATCTTCAGG
BsmAI 758 GAGAC GTCTC(1/5)
930 GAGAC
BsrI 136 CCAGT ACTGG(1/-1)
872 CCAGT
DdeI 538 CTTAG C^T[ACGT]AG
751 CTCAG
HaeIII 656 GGCC GG^CC
1043 GGCC
HincII 218 GTCGAC GT[CT]^[GA]AC
681 GTTAAC
HindII 218 GTCGAC GT[CT]^[GA]AC
681 GTTAAC
MaeII 158 ACGT A^CGT
480 ACGT
MaeIII 264 GTCAC ^GT[ACGT]AC
1063 GTAAC
NlaIV 656 GGCCCC GG[ACGT]^[ACGT]CC
828 GGTACC
NspI 35 ACATGC [GA]CATG^[CT]
124 ACATGT
PvuI 966 CGATCG CGAT^CG
979 CGATCG
TfiI 808 GAATC G^A[AT]TC
862 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AfaI AluI AspHI BfaI BmyI
BsiHKAI Bsp1286I BspDI BstF5I Cac8I
ClaI DpnII FokI HinfI HphI
MaeI MboI MboII MnlI MseI
MwoI NdeII NlaIII PleI RsaI
Sau3AI SfaNI TaqI Tru9I Tsp509I
TspRI TthHB8I