(Circular) (Six-base) MAPSORT of: pFN21AB8589.seq Check: 2899 from: 1 to: 5779 pFN21AB8589 5779 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2488 3589 278 Size: 53 83 186 936 952 1101 2468 Fragments arranged by size: 2468 1101 952 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2224 3122 1937 Size: 287 898 4594 Fragments arranged by size: 4594 898 287 Acc65I G'GTAC_C Cuts at: 2833 3100 2833 Size: 267 5512 AclI AA'CG_TT Cuts at: 1563 3906 4279 1563 Size: 2343 373 3063 Fragments arranged by size: 3063 2343 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2808 3093 3842 5226 5494 1632 Size: 1176 285 749 1384 268 1917 Fragments arranged by size: 1917 1384 1176 749 285 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5582 AflIII A'CryG_T Cuts at: 1203 2129 4678 5655 1203 Size: 926 2549 977 1327 Fragments arranged by size: 2549 1327 977 926 AgeI A'CCGG_T Cuts at: 4610 4610 Size: 5779 AhdI GACnn_n'nnGTC Cuts at: 4508 4508 Size: 5779 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5779 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5779 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2619 2651 2619 Size: 32 5747 AlwNI CAG_nnn'CTG Cuts at: 3071 3522 5094 3071 Size: 451 1572 3756 Fragments arranged by size: 3756 1572 451 ApaLI G'TGCA_C Cuts at: 3061 3836 4992 3061 Size: 775 1156 3848 Fragments arranged by size: 3848 1156 775 ApoI r'AATT_y Cuts at: 1493 2232 3088 3314 3466 5575 1493 Size: 739 856 226 152 2109 1697 Fragments arranged by size: 2109 1697 856 739 226 152 AseI AT'TA_AT Cuts at: 160 1032 4333 160 Size: 872 3301 1606 Fragments arranged by size: 3301 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5779 AvaI C'yCGr_G Cuts at: 1870 1943 2237 3104 1870 Size: 73 294 867 4545 Fragments arranged by size: 4545 867 294 73 BamHI G'GATC_C Cuts at: 3109 3476 3109 Size: 367 5412 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2833 3100 4555 618 Size: 325 217 627 1046 267 1455 1842 Fragments arranged by size: 1842 1455 1046 627 325 267 217 BanII G_rGCy'C Cuts at: 1391 2591 3098 1391 Size: 1200 507 4072 Fragments arranged by size: 4072 1200 507 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2643 2739 928 Size: 971 744 96 3968 Fragments arranged by size: 3968 971 744 96 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3970 4004 1903 Size: 34 2033 34 3678 Fragments arranged by size: 3678 2033 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3671 4881 3671 Size: 1210 4569 BclI T'GATC_A Cuts at: 1548 2026 1548 Size: 478 5301 BfrBI ATG'CAT Cuts at: 2315 2315 Size: 5779 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4390 136 Size: 107 122 71 631 605 2718 1525 Fragments arranged by size: 2718 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3038 5774 3038 Size: 2736 3043 BlpI GC'TnA_GC Cuts at: 3196 3196 Size: 5779 Bme1580I G_kGCm'C Cuts at: 1790 3065 3840 4996 1790 Size: 1275 775 1156 2573 Fragments arranged by size: 2573 1275 1156 775 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5779 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3510 4468 442 Size: 416 53 83 399 24 2093 958 1753 Fragments arranged by size: 2093 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5779 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4439 1239 Size: 3200 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2267 3904 4769 5067 2267 Size: 1637 865 298 2979 Fragments arranged by size: 2979 1637 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4442 882 Size: 617 2943 2219 Fragments arranged by size: 2943 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3570 493 Size: 711 2366 2702 Fragments arranged by size: 2702 2366 711 BsaBI GATnn'nnATC Cuts at: 5773 5773 Size: 5779 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2485 3586 3968 4603 Size: 53 83 186 936 952 1101 382 635 Cuts at: 4603 275 Size: 1451 Fragments arranged by size: 1451 1101 952 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4212 4610 4884 5031 5442 4212 Size: 398 274 147 411 4549 Fragments arranged by size: 4549 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2619 2649 1178 Size: 30 165 30 1216 30 4308 Fragments arranged by size: 4308 1216 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5779 BseYI C'CCAG_C Cuts at: 4982 5478 4982 Size: 496 5283 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2070 1163 Size: 573 334 4872 Fragments arranged by size: 4872 573 334 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2974 2987 3229 3990 5018 1540 Size: 275 186 973 13 242 761 1028 2301 Fragments arranged by size: 2301 1028 973 761 275 242 186 13 BsiHKAI G_wGCw'C Cuts at: 2218 2591 3065 3840 3925 4996 2218 Size: 373 474 775 85 1071 3001 Fragments arranged by size: 3001 1071 775 474 373 85 BsmI GAATG_Cn' Cuts at: 3290 3383 3290 Size: 93 5686 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2218 2591 3065 3098 3840 3925 4996 Size: 399 428 373 474 33 742 85 1071 Cuts at: 4996 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 474 428 399 373 85 33 BspEI T'CCGG_A Cuts at: 5442 5442 Size: 5779 BspHI T'CATG_A Cuts at: 2809 3668 5398 2809 Size: 859 1730 3190 Fragments arranged by size: 3190 1730 859 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3134 844 Size: 2290 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2603 3666 1394 Size: 429 780 1063 3507 Fragments arranged by size: 3507 1063 780 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4274 4448 65 Size: 1183 3026 174 1396 Fragments arranged by size: 3026 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4423 4610 1700 Size: 110 2613 187 2869 Fragments arranged by size: 2869 2613 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5779 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5779 BssSI C'ACGA_G Cuts at: 3839 4851 3839 Size: 1012 4767 BstAPI GCAn_nnn'nTGC Cuts at: 794 2909 794 Size: 2115 3664 BstBI TT'CG_AA Cuts at: 4583 4583 Size: 5779 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5779 BstYI r'GATC_y Cuts at: 1972 3038 3109 3476 3861 3878 5330 5416 5428 Size: 1066 71 367 385 17 1452 86 12 Cuts at: 5428 5774 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1066 385 367 346 86 71 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4288 Fragments arranged by size: 4288 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5040 Fragments arranged by size: 5040 683 56 BtsI GCAGTG_nn' Cuts at: 3295 4089 4109 3295 Size: 794 20 4965 Fragments arranged by size: 4965 794 20 ClaI AT'CG_AT Cuts at: 1554 2974 2983 2998 1554 Size: 1420 9 15 4335 Fragments arranged by size: 4335 1420 15 9 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5744 DraI TTT'AAA Cuts at: 3083 3083 Size: 5779 DraIII CAC_nnn'GTG Cuts at: 2624 2624 Size: 5779 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4786 809 Size: 507 3470 1802 Fragments arranged by size: 3470 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3226 4115 5744 Size: 54 1425 50 275 18 1396 889 1629 Cuts at: 5744 8 Size: 43 Fragments arranged by size: 1629 1425 1396 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3226 1537 Size: 275 1414 4090 Fragments arranged by size: 4090 1414 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3709 1353 Size: 2356 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4366 4740 4886 387 Size: 1064 138 2777 374 146 1280 Fragments arranged by size: 2777 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2589 2589 Size: 5779 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2808 3093 3842 4439 5226 5494 1239 Size: 393 1176 285 749 597 787 268 1524 Fragments arranged by size: 1524 1176 787 749 597 393 285 268 EcoO109I rG'GnC_Cy Cuts at: 2661 2661 Size: 5779 EcoRI G'AATT_C Cuts at: 3088 3466 3088 Size: 378 5401 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2278 2278 Size: 5779 FspI TGC'GCA Cuts at: 4285 4285 Size: 5779 HaeII r_GCGC'y Cuts at: 1678 4926 1678 Size: 3248 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2597 2629 2798 2830 2882 Size: 32 135 32 1025 32 169 32 52 Cuts at: 2882 2914 4420 4452 4494 4526 1373 Size: 32 1506 32 42 32 2626 Fragments arranged by size: 2626 1506 1025 169 135 52 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2225 2688 3123 3369 669 Size: 988 281 287 463 435 246 3079 Fragments arranged by size: 3079 988 463 435 287 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5779 HpaI GTT'AAC Cuts at: 2688 3369 2688 Size: 681 5098 KpnI G_GTAC'C Cuts at: 2837 3104 2837 Size: 267 5512 MfeI C'AATT_G Cuts at: 3378 3378 Size: 5779 MluI A'CGCG_T Cuts at: 5655 5655 Size: 5779 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4893 5077 1305 Size: 3588 184 2007 Fragments arranged by size: 3588 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3957 Fragments arranged by size: 3957 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2411 3737 4096 4255 518 Size: 648 1245 1326 359 159 2042 Fragments arranged by size: 2042 1326 1245 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2774 3195 3872 5020 5265 1606 Size: 1168 421 677 1148 245 2120 Fragments arranged by size: 2120 1168 1148 677 421 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4288 Fragments arranged by size: 4288 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5779 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5779 NotI GC'GGCC_GC Cuts at: 3226 3226 Size: 5779 NsiI A_TGCA'T Cuts at: 2317 2317 Size: 5779 NspI r_CATG'y Cuts at: 1132 1229 2044 2133 3137 4682 1132 Size: 97 815 89 1004 1545 2229 Fragments arranged by size: 2229 1545 1004 815 97 89 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5779 PciI A'CATG_T Cuts at: 2129 4678 2129 Size: 2549 3230 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5779 PmeI GTTT'AAAC Cuts at: 3083 3083 Size: 5779 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2619 2651 3848 3880 5361 5393 2619 Size: 32 1197 32 1481 32 3005 Fragments arranged by size: 3005 1481 1197 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5779 PsiI TTA'TAA Cuts at: 3349 3349 Size: 5779 PstI C_TGCA'G Cuts at: 830 2905 3131 830 Size: 2075 226 3478 Fragments arranged by size: 3478 2075 226 PvuI CG_AT'CG Cuts at: 2001 2974 2987 2001 Size: 973 13 4793 Fragments arranged by size: 4793 973 13 PvuII CAG'CTG Cuts at: 2774 2774 Size: 5779 SacI G_AGCT'C Cuts at: 2591 2591 Size: 5779 SalI G'TCGA_C Cuts at: 1936 2223 3121 1936 Size: 287 898 4594 Fragments arranged by size: 4594 898 287 SbfI CC_TGCA'GG Cuts at: 3131 3131 Size: 5779 ScaI AGT'ACT Cuts at: 4027 5739 4027 Size: 1712 4067 SfcI C'TryA_G Cuts at: 826 1045 2901 3127 4262 4943 5134 826 Size: 219 1856 226 1135 681 191 1471 Fragments arranged by size: 1856 1471 1135 681 226 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5779 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5779 SmaI CCC'GGG Cuts at: 1872 3106 1872 Size: 1234 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2282 3883 4784 5046 820 Size: 197 926 339 1601 901 262 1553 Fragments arranged by size: 1601 1553 926 901 339 262 197 SnaBI TAC'GTA Cuts at: 493 3570 493 Size: 3077 2702 SpeI A'CTAG_T Cuts at: 152 2363 152 Size: 2211 3568 SphI G_CATG'C Cuts at: 1132 3137 1132 Size: 2005 3774 SspI AAT'ATT Cuts at: 5 52 3703 5 Size: 47 3651 2081 Fragments arranged by size: 3651 2081 47 StuI AGG'CCT Cuts at: 1517 3049 1517 Size: 1532 4247 StyI C'CwwG_G Cuts at: 513 1065 2004 3218 513 Size: 552 939 1214 3074 Fragments arranged by size: 3074 1214 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2328 3806 3823 3976 4161 4606 325 Size: 1255 748 1478 17 153 185 445 1498 Fragments arranged by size: 1498 1478 1255 748 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4025 5737 96 Size: 274 80 33 51 1443 2048 1712 138 Fragments arranged by size: 2048 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5739 5771 5739 Size: 32 5747 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5779 XbaI T'CTAG_A Cuts at: 3041 3115 3041 Size: 74 5705 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5779 XmaI C'CCGG_G Cuts at: 1870 3104 1870 Size: 1234 4545 XmnI GAAnn'nnTTC Cuts at: 3908 3908 Size: 5779 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2486 3587 276 Size: 53 83 186 936 952 1101 2468 Fragments arranged by size: 2468 1101 952 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI