Restriction map for
pFN21AB8585
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccII AcyI AflIII
AgeI AlwNI Aor51HI ApaI AscI
Asp718I AspI AvaI AviII AvrII
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BcnI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsiEI
BsiWI BsmBI BsoBI BspDI BspLU11I
BsrBI BsrFI BssHII BssSI Bst1107I
BstEII BstPI BstUI BstXI BstZ17I
CelII Cfr10I ClaI CpoI DraIII
DrdI EagI EclXI Eco47III Eco52I
EcoO65I EcoRV EcoT22I FseI FspI
HaeII HgaI Hin1I HpaI KasI
KpnI KspI MamI MfeI MluI
MunI MvnI NaeI NarI NciI
NgoMIV NotI NruI NsiI PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PstI PvuI
RsrII SacI SacII SalI SapI
SbfI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
SwaI Tth111I Van91I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 66 enzymes) #
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AccI 235 GTCTAC GT^[AC][GT]AC
AccIII 158 TCCGGA T^CCGGA
AclI 707 AACGTT AA^CGTT
AflII 1740 CTTAAG C^TTAAG
AhdI 1285 GACTACGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2018 GTGCAC G^TGCAC
AlwI 1438 GATCC GGATC(4/5)
AocI 22 CCTCAGG CC^T[ACGT]AGG
ApaLI 2018 GTGCAC G^TGCAC
AseI 1063 ATTAAT AT^TAAT
AsnI 1063 ATTAAT AT^TAAT
Asp700I 1914 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1285 GACTACGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 203 TGGCCA TGG^CCA
BciVI 1390 GTATCC GTATCC(6/5)
BfrI 1740 CTTAAG C^TTAAG
BglII 276 AGATCT A^GATCT
BsaI 2377 GGTCTC GGTCTC(1/5)
BsaWI 158 TCCGGA [AT]^CCGG[AT]
BseAI 158 TCCGGA T^CCGGA
BseRI 2062 GAGGAG GAGGAG(10/8)
BsmI 587 GAATGC GAATGC(1/-1)
Bsp1407I 291 TGTACA T^GTACA
BspEI 158 TCCGGA T^CCGGA
BspMI 990 GCAGGT ACCTGC(4/8)
BsrDI 897 CATTGC GCAATG(2/0)
BsrGI 291 TGTACA T^GTACA
BstAPI 1762 GCACTAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 446 TTCGAA TT^CGAA
BstYI 276 AGATCT [GA]^GATC[CT]
Bsu36I 22 CCTCAGG CC^T[ACGT]AGG
DraI 2226 TTTAAA TTT^AAA
DraII 1502 AGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 203 TGGCCA [CT]^GGCC[GA]
Eam1105I 1285 GACTACGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 22 CCTCAGG CC^T[ACGT]AGG
EcoNI 484 CCTGATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1502 AGGACCC [GA]G^G[ACGT]CC[CT]
FauI 2340 GCGGG CCCGC(4/6)
HapII 159 CCGG C^CGG
HincII 1216 GTTGAC GT[CT]^[GA]AC
HindII 1216 GTTGAC GT[CT]^[GA]AC
HindIII 112 AAGCTT A^AGCTT
HpaII 159 CCGG C^CGG
MflI 276 AGATCT [GA]^GATC[CT]
MluNI 203 TGGCCA TGG^CCA
MroI 158 TCCGGA T^CCGGA
MscI 203 TGGCCA TGG^CCA
MslI 868 CACCAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 159 CCGG C^CGG
NcoI 536 CCATGG C^CATGG
NdeI 1808 CATATG CA^TATG
NheI 1872 GCTAGC G^CTAGC
NspV 446 TTCGAA TT^CGAA
PpuMI 1502 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 1063 ATTAAT AT^TAAT
Psp1406I 707 AACGTT AA^CGTT
PvuII 1612 CAGCTG CAG^CTG
SexAI 1364 ACCAGGT A^CC[AT]GGT
SfuI 446 TTCGAA TT^CGAA
SspBI 291 TGTACA T^GTACA
SspI 1231 AATATT AAT^ATT
Tsp45I 521 GTGAC ^GT[GC]AC
XcmI 1376 CCATAATGAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 276 AGATCT [GA]^GATC[CT]
XmnI 1914 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
BbsI 1429 GAAGAC GAAGAC(2/6)
2254 GAAGAC
BglI 55 GCCATACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1466 GCCTTGTGGGC
BpmI 826 CTCCAG CTGGAG(16/14)
2320 CTGGAG
BpuAI 1429 GAAGAC GAAGAC(2/6)
2254 GAAGAC
BspHI 1794 TCATGA T^CATGA
1935 TCATGA
DsaI 411 CCGTGG C^C[GA][CT]GG
536 CCATGG
EcoRI 1635 GAATTC G^AATTC
1813 GAATTC
EcoT14I 316 CCAAGG C^C[AT][AT]GG
536 CCATGG
MaeII 708 ACGT A^CGT
1396 ACGT
NspI 132 ACATGC [GA]CATG^[CT]
1022 ACATGC
PleI 1950 GACTC GAGTC(4/5)
1291 GAGTC
RcaI 1794 TCATGA T^CATGA
1935 TCATGA
ScaI 1277 AGTACT AGT^ACT
1517 AGTACT
StyI 316 CCAAGG C^C[AT][AT]GG
536 CCATGG
XbaI 1012 TCTAGA T^CTAGA
1116 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AspHI AvaII BbvI BclI BfaI
BmyI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII EarI EcoRII
FbaI Fnu4HI FokI HaeIII HhaI
HinP1I HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MspA1I MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I