Restriction map for pFN21AB8585
 (Circular) (Six-base) MAPSORT of: pFN21AB8585.seq  Check: 1131  from: 1  to: 7093



pFN21AB8585  7093 bp

 With 182 enzymes: SgfI * 

                        October 11, 2011 14:05  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2986    2986 
   Size:       7093
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    4903     278 
   Size:         53      83     186     936    3367    2468

  Fragments arranged by size: 

                3367    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2241    4436    1937 
   Size:        304    2195    4594

  Fragments arranged by size: 

                4594    2195     304
 
Acc65I G'GTAC_C

Cuts at:   4414    4414 
   Size:       7093
 
AclI AA'CG_TT

Cuts at:   1563    2713    5220    5593    1563 
   Size:       1150    2507     373    3063

  Fragments arranged by size: 

                3063    2507    1150     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2450    3018    3910    5156    6540    6808    1632 
   Size:        818     568     892    1246    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1246     892     818     568     268
 
AflII C'TTAA_G

Cuts at:    820    1017    3745     820 
   Size:        197    2728    4168

  Fragments arranged by size: 

                4168    2728     197
 
AflIII A'CryG_T

Cuts at:   1203    5992    6969    1203 
   Size:       4789     977    1327

  Fragments arranged by size: 

                4789    1327     977
 
AgeI A'CCGG_T

Cuts at:   5924    5924 
   Size:       7093
 
AhdI GACnn_n'nnGTC

Cuts at:   3295    5822    3295 
   Size:       2527    4566
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       7093
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    4147    1184 
   Size:       2963    4130
 
AlwNI CAG_nnn'CTG

Cuts at:   4836    6408    4836 
   Size:       1572    5521
 
ApaLI G'TGCA_C

Cuts at:   4023    5150    6306    4023 
   Size:       1127    1156    4810

  Fragments arranged by size: 

                4810    1156    1127
 
ApoI r'AATT_y

Cuts at:   1493    2655    2671    2822    3388    3640    3818    4170    4402 
   Size:       1162      16     151     566     252     178     352     232

Cuts at:   4402    4628    4780    6889    1493 
   Size:        226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1162     566     352     252     232     226
                 178     152     151      16
 
AseI AT'TA_AT

Cuts at:    160    1032    3069    5647     160 
   Size:        872    2037    2578    1606

  Fragments arranged by size: 

                2578    2037    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       7093
 
AvaI C'yCGr_G

Cuts at:   1870    1943    4418    1870 
   Size:         73    2475    4545

  Fragments arranged by size: 

                4545    2475      73
 
BamHI G'GATC_C

Cuts at:   4423    4790    4423 
   Size:        367    6726
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    4414    5869     618 
   Size:        325     217     627    2627    1455    1842

  Fragments arranged by size: 

                2627    1842    1455     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    4412    1391 
   Size:       3021    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    3441    4266     928 
   Size:        971    1542     825    3755

  Fragments arranged by size: 

                3755    1542     971     825
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    1987    2021    5284    5318    1903 
   Size:         34      50      34    3263      34    3678

  Fragments arranged by size: 

                3678    3263      50      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3406    4985    6195    3406 
   Size:       1579    1210    4304

  Fragments arranged by size: 

                4304    1579    1210
 
BclI T'GATC_A

Cuts at:   1548    2337    3717    3894    3997    1548 
   Size:        789    1380     177     103    4644

  Fragments arranged by size: 

                4644    1380     789     177     103
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2066    3477    5704 
   Size:        107     122      71     631     605     394    1411    2227

Cuts at:   5704     136 
   Size:       1525

  Fragments arranged by size: 

                2227    1525    1411     631     605     394     122     107
                  71
 
BglII A'GATC_T

Cuts at:   2281    7088    2281 
   Size:       4807    2286
 
BlpI GC'TnA_GC

Cuts at:   4510    4510 
   Size:       7093
 
Bme1580I G_kGCm'C

Cuts at:   1790    3967    4027    5154    6310    1790 
   Size:       2177      60    1127    1156    2573

  Fragments arranged by size: 

                2573    2177    1156    1127      60
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       7093
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    4824    5782     442 
   Size:        416      53      83     399      24    3407     958    1753

  Fragments arranged by size: 

                3407    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    3881    1056 
   Size:       2825    4268
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4280    4280 
   Size:       7093
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2816    4346    5753    1239 
   Size:       1577    1530    1407    2579

  Fragments arranged by size: 

                2579    1577    1530    1407
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2455    2763    3348    5218    6083    6381    2455 
   Size:        308     585    1870     865     298    3167

  Fragments arranged by size: 

                3167    1870     865     585     308     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4388    5756     882 
   Size:        617    2889    1368    2219

  Fragments arranged by size: 

                2889    2219    1368     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    4884     493 
   Size:        711    3680    2702

  Fragments arranged by size: 

                3680    2702     711
 
BsaBI GATnn'nnATC

Cuts at:   7087    7087 
   Size:       7093
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    4900    5282    5917     275 
   Size:         53      83     186     936    3367     382     635    1451

  Fragments arranged by size: 

                3367    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   2163    5526    5924    6198    6345    6756    2163 
   Size:       3363     398     274     147     411    2500

  Fragments arranged by size: 

                3363    2500     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    6868

  Fragments arranged by size: 

                6868     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    4082    1186 
   Size:       2896    4197
 
BseYI C'CCAG_C

Cuts at:   2959    3776    6296    6792    2959 
   Size:        817    2520     496    3260

  Fragments arranged by size: 

                3260    2520     817     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2210    3201    4175    1163 
   Size:        573     474     991     974    4081

  Fragments arranged by size: 

                4081     991     974     573     474
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    4543    5304    6332    1540 
   Size:        275     186    2542     761    1028    2301

  Fragments arranged by size: 

                2542    2301    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3769    4019    4027    5154    5239    6310    3769 
   Size:        250       8    1127      85    1071    4552

  Fragments arranged by size: 

                4552    1127    1071     250      85       8
 
BsmI GAATG_Cn'

Cuts at:   2598    4604    4697    2598 
   Size:       2006      93    4994

  Fragments arranged by size: 

                4994    2006      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    3769    3967    4019    4027    4412    5154    5239 
   Size:        399    1979     198      52       8     385     742      85

Cuts at:   5239    6310    1391 
   Size:       1071    2174

  Fragments arranged by size: 

                2174    1979    1071     742     399     385     198      85
                  52       8
 
BspEI T'CCGG_A

Cuts at:   2163    6756    2163 
   Size:       4593    2500
 
BspHI T'CATG_A

Cuts at:   3799    3940    4982    6712    3799 
   Size:        141    1042    1730    4180

  Fragments arranged by size: 

                4180    1730    1042     141
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2986    4448     844 
   Size:       2142    1462    3489

  Fragments arranged by size: 

                3489    2142    1462
 
BsrBI CCG'CTC

Cuts at:   1394    1823    4980    1394 
   Size:        429    3157    3507

  Fragments arranged by size: 

                3507    3157     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2901    5588    5762      65 
   Size:       1183    1653    2687     174    1396

  Fragments arranged by size: 

                2687    1653    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    5737    5924    1700 
   Size:        110    3927     187    2869

  Fragments arranged by size: 

                3927    2869     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    2296      96 
   Size:       2200    4893
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       7093
 
BssSI C'ACGA_G

Cuts at:   5153    6165    5153 
   Size:       1012    6081
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    3773     794 
   Size:       2979    4114
 
BstBI TT'CG_AA

Cuts at:   2452    5897    2452 
   Size:       3445    3648
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       7093
 
BstYI r'GATC_y

Cuts at:   1972    2281    4423    4790    5175    5192    6644    6730    6742 
   Size:        309    2142     367     385      17    1452      86      12

Cuts at:   6742    7088    1972 
   Size:        346    1977

  Fragments arranged by size: 

                2142    1977    1452     385     367     346     309      86
                  17      12
 
Bsu36I CC'TnA_GG

Cuts at:   2028    2028 
   Size:       7093
 
BtgI C'CryG_G

Cuts at:    513    1065    2416    2541     513 
   Size:        552    1351     125    5065

  Fragments arranged by size: 

                5065    1351     552     125
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    6354

  Fragments arranged by size: 

                6354     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3587    4609    5403    5423    3587 
   Size:       1022     794      20    5257

  Fragments arranged by size: 

                5257    1022     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       7093
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    7058
 
DraI TTT'AAA

Cuts at:   4233    4397    4233 
   Size:        164    6929
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    6100     809 
   Size:        507    4784    1802

  Fragments arranged by size: 

                4784    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2208    4540    5429 
   Size:         54    1425      50     275      18     378    2332     889

Cuts at:   5429    7058       8 
   Size:       1629      43

  Fragments arranged by size: 

                2332    1629    1425     889     378     275      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    4540    1537 
   Size:        275    2728    4090

  Fragments arranged by size: 

                4090    2728     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2090    3927    4037    5023    1353 
   Size:        737    1837     110     986    3423

  Fragments arranged by size: 

                3423    1837     986     737     110
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    5680    6054    6200     387 
   Size:       1064     138    4091     374     146    1280

  Fragments arranged by size: 

                4091    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2450    2816    3018    3910    4346    5156    5753 
   Size:        393     818     366     202     892     436     810     597

Cuts at:   5753    6540    6808    1239 
   Size:        787     268    1524

  Fragments arranged by size: 

                1524     892     818     810     787     597     436     393
                 366     268     202
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2493    2493 
   Size:       7093
 
EcoO109I rG'GnC_Cy

Cuts at:   3508    3508 
   Size:       7093
 
EcoRI G'AATT_C

Cuts at:   3640    3818    4402    4780    3640 
   Size:        178     584     378    5953

  Fragments arranged by size: 

                5953     584     378     178
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   4175    4175 
   Size:       7093
 
FspI TGC'GCA

Cuts at:   5599    5599 
   Size:       7093
 
HaeII r_GCGC'y

Cuts at:   1678    6240    1678 
   Size:       4562    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2827    2859    2940    2972    3032 
   Size:         32     135      32    1255      32      81      32      60

Cuts at:   3032    3064    3281    3313    3397    3429    5734    5766    5808 
   Size:         32     217      32      84      32    2305      32      42

Cuts at:   5808    5840    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    2305    1255     217     135      84      81      60
                  42      32      32      32      32      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3223    4437    4683     669 
   Size:        988     281    1285    1214     246    3079

  Fragments arranged by size: 

                3079    1285    1214     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2117     748 
   Size:       1369    5724
 
HpaI GTT'AAC

Cuts at:   4683    4683 
   Size:       7093
 
KpnI G_GTAC'C

Cuts at:   4418    4418 
   Size:       7093
 
MfeI C'AATT_G

Cuts at:   4692    4692 
   Size:       7093
 
MluI A'CGCG_T

Cuts at:   6969    6969 
   Size:       7093
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2326    2673    6207    6391    1305 
   Size:       1021     347    3534     184    2007

  Fragments arranged by size: 

                3534    2007    1021     347     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2210      10 
   Size:         54    1425     343     378    4893

  Fragments arranged by size: 

                4893    1425     378     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2877    5051    5410    5569     518 
   Size:        648    1711    2174     359     159    2042

  Fragments arranged by size: 

                2174    2042    1711     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2963    3574    3619    4509    5186    6334    6579    1606 
   Size:       1357     611      45     890     677    1148     245    2120

  Fragments arranged by size: 

                2120    1357    1148     890     677     611     245      45
            
 
NcoI C'CATG_G

Cuts at:    513    1065    2541     513 
   Size:        552    1476    5065

  Fragments arranged by size: 

                5065    1476     552
 
NdeI CA'TA_TG

Cuts at:    387    3814     387 
   Size:       3427    3666
 
NheI G'CTAG_C

Cuts at:   1052    3877    1052 
   Size:       2825    4268
 
NotI GC'GGCC_GC

Cuts at:   4540    4540 
   Size:       7093
 
NspI r_CATG'y

Cuts at:   1132    1229    2141    3031    4451    5996    1132 
   Size:         97     912     890    1420    1545    2229

  Fragments arranged by size: 

                2229    1545    1420     912     890      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       7093
 
PciI A'CATG_T

Cuts at:   5992    5992 
   Size:       7093
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       7093
 
PfoI T'CCnGG_A

Cuts at:   2378    2378 
   Size:       7093
 
PmeI GTTT'AAAC

Cuts at:   4397    4397 
   Size:       7093
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5162    5194    6675    6707    5162 
   Size:         32    1481      32    5548

  Fragments arranged by size: 

                5548    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   3508    3508 
   Size:       7093
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       7093
 
PsiI TTA'TAA

Cuts at:   3345    4663    3345 
   Size:       1318    5775
 
PstI C_TGCA'G

Cuts at:    830    4445     830 
   Size:       3615    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       7093
 
PvuII CAG'CTG

Cuts at:   3619    3619 
   Size:       7093
 
SalI G'TCGA_C

Cuts at:   1936    4435    1936 
   Size:       2499    4594
 
SbfI CC_TGCA'GG

Cuts at:   4445    4445 
   Size:       7093
 
ScaI AGT'ACT

Cuts at:   3284    3524    5341    7053    3284 
   Size:        240    1817    1712    3324

  Fragments arranged by size: 

                3324    1817    1712     240
 
SexAI A'CCwGG_T

Cuts at:   3369    3369 
   Size:       7093
 
SfcI C'TryA_G

Cuts at:    826    1045    2761    3003    3429    4441    5576    6257    6448 
   Size:        219    1716     242     426    1012    1135     681     191

Cuts at:   6448     826 
   Size:       1471

  Fragments arranged by size: 

                1716    1471    1135    1012     681     426     242     219
                 191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       7093
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       7093
 
SmaI CCC'GGG

Cuts at:   1872    4420    1872 
   Size:       2548    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2434    2778    3363    3745    5197    6098 
   Size:        197     926     491     344     585     382    1452     901

Cuts at:   6098    6360     820 
   Size:        262    1553

  Fragments arranged by size: 

                1553    1452     926     901     585     491     382     344
                 262     197
 
SnaBI TAC'GTA

Cuts at:    493    4884     493 
   Size:       4391    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       7093
 
SphI G_CATG'C

Cuts at:   1132    4451    1132 
   Size:       3319    3774
 
SspI AAT'ATT

Cuts at:      5      52    3238    5017       5 
   Size:         47    3186    1779    2081

  Fragments arranged by size: 

                3186    2081    1779      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       7093
 
StyI C'CwwG_G

Cuts at:    513    1065    2321    2541    4532     513 
   Size:        552    1256     220    1991    3074

  Fragments arranged by size: 

                3074    1991    1256     552     220
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2575    3285    5120    5137    5290    5475    5920 
   Size:       1255     995     710    1835      17     153     185     445

Cuts at:   5920     325 
   Size:       1498

  Fragments arranged by size: 

                1835    1498    1255     995     710     445     185     153
                  17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2296    2551    3282 
   Size:        274      80      33      51    1443     319     255     731

Cuts at:   3282    3522    5339    7051      96 
   Size:        240    1817    1712     138

  Fragments arranged by size: 

                1817    1712    1443     731     319     274     255     240
                 138      80      51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2259    2291    3946    3978    7053    7085    2259 
   Size:         32    1655      32    3075      32    2267

  Fragments arranged by size: 

                3075    2267    1655      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       7093
 
XbaI T'CTAG_A

Cuts at:   3017    3121    4429    3017 
   Size:        104    1308    5681

  Fragments arranged by size: 

                5681    1308     104
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   3388    3388 
   Size:       7093
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       7093
 
XmaI C'CCGG_G

Cuts at:   1870    4418    1870 
   Size:       2548    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3923    5222    3923 
   Size:       1299    5794
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    4901     276 
   Size:         53      83     186     936    3367    2468

  Fragments arranged by size: 

                3367    2468     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NspI
     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI
     PsiI     PstI     PvuI    PvuII     SalI     SbfI     ScaI    SexAI
     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I   DraIII  EcoICRI
    EcoRV     FseI    FspAI     KasI     NaeI     NarI   NgoMIV     NruI
     NsiI     PacI     PmlI   PspOMI    PspXI     PsrI    RsrII     SacI
    SacII    SanDI     SapI     SfiI     SfoI     SrfI     SwaI
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