Restriction map for
pFN21AB8466
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BamHI BbrPI BciVI BclI
BfaI BfrI BglII BlnI BpmI
BsaI BseAI BsgI BsiWI BsmAI
BsmBI BsmFI Bsp1407I BspDI BspEI
BspLU11I BsrDI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstYI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI Eam1105I Eco47III Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI HgaI HindIII HinfI
HpaI KpnI MaeI MfeI MflI
MluI MroI MseI MunI NcoI
NdeI NheI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RsrII
SalI SbfI ScaI SfcI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI TfiI
Tru9I Tsp509I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AcyI 710 GGCGCC G[GA]^CG[CT]C
AfaI 436 GTAC GT^AC
AflIII 89 ACGTGT A^C[GA][CT]GT
AlwI 138 GATCC GGATC(4/5)
AlwNI 38 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
BalI 1175 TGGCCA TGG^CCA
BbeI 710 GGCGCC GGCGC^C
BbvCI 509 CCTCAGC CCTCAGC(-5/-2)
BcgI 858 CGAGGAGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 701 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 701 GCTCAGC GC^T[ACGT]AGC
BsaAI 820 TACGTG [CT]AC^GT[GA]
BsaBI 1236 GATGCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 710 GGCGCC G[GA]^CG[CT]C
BsaWI 471 TCCGGT [AT]^CCGG[AT]
BsmI 48 GAATGC GAATGC(1/-1)
BspHI 407 TCATGA T^CATGA
BssSI 729 CTCGTG CACGAG(-5/-1)
BstAPI 389 GCATATTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1166 CCAGCAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 701 GCTCAGC GC^T[ACGT]AGC
Hin1I 710 GGCGCC G[GA]^CG[CT]C
HincII 835 GTCAAC GT[CT]^[GA]AC
HindII 835 GTCAAC GT[CT]^[GA]AC
KasI 710 GGCGCC G^GCGCC
KspI 555 CCGCGG CCGC^GG
MamI 1236 GATGCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1175 TGGCCA TGG^CCA
MscI 1175 TGGCCA TGG^CCA
NarI 710 GGCGCC GG^CGCC
NotI 1158 GCGGCCGC GC^GGCCGC
PpuMI 977 GGGTCCT [GA]G^G[AT]CC[CT]
RcaI 407 TCATGA T^CATGA
RsaI 436 GTAC GT^AC
SacI 271 GAGCTC GAGCT^C
SacII 555 CCGCGG CCGC^GG
SexAI 152 ACCTGGT A^CC[AT]GGT
SfoI 710 GGCGCC GGC^GCC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 148 GTCTAC GT^[AC][GT]AC
241 GTCTAC
ApaI 99 GGGCCC GGGCC^C
1027 GGGCCC
AviII 1004 TGCGCA TGC^GCA
1238 TGCGCA
BbsI 1038 GAAGAC GAAGAC(2/6)
538 GTCTTC
BglI 52 GCCTCCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
781 GCCGTGGTGGC
BpuAI 1038 GAAGAC GAAGAC(2/6)
538 GTCTTC
BspMI 568 ACCTGC ACCTGC(4/8)
677 ACCTGC
BsrBI 447 CCGCTC CCGCTC(-3/-3)
1156 GAGCGG
BsrFI 591 GCCGGC [GA]^CCGG[CT]
610 GCCGGC
Cfr10I 591 GCCGGC [GA]^CCGG[CT]
610 GCCGGC
DpnII 138 GATC ^GATC
1128 GATC
EarI 274 CTCTTC CTCTTC(1/4)
793 CTCTTC
FspI 1004 TGCGCA TGC^GCA
1238 TGCGCA
Ksp632I 274 CTCTTC CTCTTC(1/4)
793 CTCTTC
MboI 138 GATC ^GATC
1128 GATC
NaeI 591 GCCGGC GCC^GGC
610 GCCGGC
NdeII 138 GATC ^GATC
1128 GATC
NgoMIV 591 GCCGGC G^CCGGC
610 GCCGGC
NspI 5 ACATGC [GA]CATG^[CT]
575 ACATGC
PvuII 704 CAGCTG CAG^CTG
915 CAGCTG
SapI 273 GCTCTTC GCTCTTC(1/4)
792 GCTCTTC
Sau3AI 138 GATC ^GATC
1128 GATC
Tsp45I 232 GTCAC ^GT[GC]AC
763 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AccII AciI AluI AspHI AvaI
AvaII BanI BanII BbvI BcnI
BmyI BsaJI BseRI BsiEI BsiHKAI
BsiYI BslI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DraII DsaI
EaeI EagI EclXI Eco52I EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MaeII MaeIII MboII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NlaIII NlaIV
Sau96I ScrFI SfaNI TseI TspRI