(Circular) (Six-base) MAPSORT of: pFN21AB8466.seq Check: 385 from: 1 to: 5959 pFN21AB8466 5959 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2582 2582 Size: 5959 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3769 278 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2154 2247 3302 1937 Size: 217 93 1055 4594 Fragments arranged by size: 4594 1055 217 93 Acc65I G'GTAC_C Cuts at: 3280 3280 Size: 5959 AclI AA'CG_TT Cuts at: 1563 4086 4459 1563 Size: 2523 373 3063 Fragments arranged by size: 3063 2523 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2217 4022 5406 5674 1632 Size: 585 1805 1384 268 1917 Fragments arranged by size: 1917 1805 1384 585 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5762 AflIII A'CryG_T Cuts at: 1203 2094 4858 5835 1203 Size: 891 2764 977 1327 Fragments arranged by size: 2764 1327 977 891 AgeI A'CCGG_T Cuts at: 4790 4790 Size: 5959 AhdI GACnn_n'nnGTC Cuts at: 4688 4688 Size: 5959 AleI CACnn'nnGTG Cuts at: 1166 2734 1166 Size: 1568 4391 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2752 3002 1184 Size: 1568 250 4141 Fragments arranged by size: 4141 1568 250 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2306 2338 2306 Size: 32 5927 AlwNI CAG_nnn'CTG Cuts at: 2048 3702 5274 2048 Size: 1654 1572 2733 Fragments arranged by size: 2733 1654 1572 ApaI G_GGCC'C Cuts at: 2108 3036 2108 Size: 928 5031 ApaLI G'TGCA_C Cuts at: 4016 5172 4016 Size: 1156 4803 ApoI r'AATT_y Cuts at: 1493 3268 3494 3646 5755 1493 Size: 1775 226 152 2109 1697 Fragments arranged by size: 2109 1775 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4513 160 Size: 872 3481 1606 Fragments arranged by size: 3481 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 AvaI C'yCGr_G Cuts at: 1870 1943 2548 2591 2861 3284 1870 Size: 73 605 43 270 423 4545 Fragments arranged by size: 4545 605 423 270 73 43 BamHI G'GATC_C Cuts at: 3289 3656 3289 Size: 367 5592 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2715 2728 3146 3280 4735 Size: 325 217 627 928 13 418 134 1455 Cuts at: 4735 618 Size: 1842 Fragments arranged by size: 1842 1455 928 627 418 325 217 134 13 BanII G_rGCy'C Cuts at: 1391 2108 2280 2886 3024 3036 3278 1391 Size: 717 172 606 138 12 242 4072 Fragments arranged by size: 4072 717 606 242 172 138 12 BbeI G_GCGC'C Cuts at: 2719 2719 Size: 5959 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2536 3050 928 Size: 971 637 514 3837 Fragments arranged by size: 3837 971 637 514 BbvCI CC'TCA_GC Cuts at: 2515 2515 Size: 5959 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2852 2886 4150 4184 1903 Size: 34 915 34 1264 34 3678 Fragments arranged by size: 3678 1264 915 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3851 5061 3851 Size: 1210 4749 BclI T'GATC_A Cuts at: 1548 1548 Size: 5959 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2063 2792 4570 Size: 107 122 71 631 605 391 729 1778 Cuts at: 4570 136 Size: 1525 Fragments arranged by size: 1778 1525 729 631 605 391 122 107 71 BglII A'GATC_T Cuts at: 5954 5954 Size: 5959 BlpI GC'TnA_GC Cuts at: 2707 3376 2707 Size: 669 5290 Bme1580I G_kGCm'C Cuts at: 1790 2108 2559 2731 2855 3036 4020 5176 1790 Size: 318 451 172 124 181 984 1156 2573 Fragments arranged by size: 2573 1156 984 451 318 181 172 124 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5959 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2651 3231 3690 Size: 416 53 83 399 24 1234 580 459 Cuts at: 3690 4648 442 Size: 958 1753 Fragments arranged by size: 1753 1234 958 580 459 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5959 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2074 2074 Size: 5959 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4619 1239 Size: 3380 2579 Bpu10I CC'TnA_GC Cuts at: 2515 2515 Size: 5959 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4084 4949 5247 4084 Size: 865 298 4796 Fragments arranged by size: 4796 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4622 882 Size: 617 3123 2219 Fragments arranged by size: 3123 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2827 3750 493 Size: 711 1623 923 2702 Fragments arranged by size: 2702 1623 923 711 BsaBI GATnn'nnATC Cuts at: 3245 5953 3245 Size: 2708 3251 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2716 3766 4148 4783 Size: 53 83 186 936 1183 1050 382 635 Cuts at: 4783 275 Size: 1451 Fragments arranged by size: 1451 1183 1050 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2476 4392 4790 5064 5211 5622 2476 Size: 1916 398 274 147 411 2813 Fragments arranged by size: 2813 1916 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5734 Fragments arranged by size: 5734 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2050 2879 3065 3068 3077 3080 3083 3095 Size: 864 829 186 3 9 3 3 12 Cuts at: 3095 1186 Size: 4050 Fragments arranged by size: 4050 864 829 186 12 9 3 3 3 BseYI C'CCAG_C Cuts at: 2182 2287 2711 3170 5162 5658 2182 Size: 105 424 459 1992 496 2483 Fragments arranged by size: 2483 1992 496 459 424 105 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5386 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2637 3121 3167 3409 4170 5198 Size: 275 186 636 484 46 242 761 1028 Cuts at: 5198 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 636 484 275 242 186 46 BsiHKAI G_wGCw'C Cuts at: 2280 2573 2808 2832 4020 4105 5176 2280 Size: 293 235 24 1188 85 1071 3063 Fragments arranged by size: 3063 1188 1071 293 235 85 24 BsmI GAATG_Cn' Cuts at: 2059 3470 3563 2059 Size: 1411 93 4455 Fragments arranged by size: 4455 1411 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2108 2280 2559 2573 2731 2808 2832 Size: 399 318 172 279 14 158 77 24 Cuts at: 2832 2855 2886 3024 3036 3278 4020 4105 5176 Size: 23 31 138 12 242 742 85 1071 Cuts at: 5176 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 318 279 242 172 158 138 85 77 31 24 23 14 12 BspEI T'CCGG_A Cuts at: 5622 5622 Size: 5959 BspHI T'CATG_A Cuts at: 2412 3848 5578 2412 Size: 1436 1730 2793 Fragments arranged by size: 2793 1730 1436 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2582 2691 3314 844 Size: 1738 109 623 3489 Fragments arranged by size: 3489 1738 623 109 BsrBI CCG'CTC Cuts at: 1394 1823 2454 3163 3846 1394 Size: 429 631 709 683 3507 Fragments arranged by size: 3507 709 683 631 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4454 4628 65 Size: 1183 3206 174 1396 Fragments arranged by size: 3206 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2596 2615 4603 4790 1700 Size: 110 786 19 1988 187 2869 Fragments arranged by size: 2869 1988 786 187 110 19 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5959 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5959 BssSI C'ACGA_G Cuts at: 2734 4019 5031 2734 Size: 1285 1012 3662 Fragments arranged by size: 3662 1285 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2400 794 Size: 1606 4353 BstBI TT'CG_AA Cuts at: 4763 4763 Size: 5959 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5959 BstXI CCAn_nnnn'nTGG Cuts at: 3178 3178 Size: 5959 BstYI r'GATC_y Cuts at: 1972 3289 3656 4041 4058 5510 5596 5608 5954 Size: 1317 367 385 17 1452 86 12 346 Cuts at: 5954 1972 Size: 1977 Fragments arranged by size: 1977 1452 1317 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2436 2520 2535 2560 2787 513 Size: 552 939 432 84 15 25 227 3685 Fragments arranged by size: 3685 939 552 432 227 84 25 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5220 Fragments arranged by size: 5220 683 56 BtsI GCAGTG_nn' Cuts at: 2591 2855 3475 4269 4289 2591 Size: 264 620 794 20 4261 Fragments arranged by size: 4261 794 620 264 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5959 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5924 DraI TTT'AAA Cuts at: 3263 3263 Size: 5959 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4966 809 Size: 507 3650 1802 Fragments arranged by size: 3650 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2124 2223 2268 Size: 54 1425 50 275 18 294 99 45 Cuts at: 2268 2304 2433 2634 2784 3118 3164 3180 3406 Size: 36 129 201 150 334 46 16 226 Cuts at: 3406 4295 5924 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 334 294 275 226 201 150 129 99 54 50 46 45 43 36 18 16 EagI C'GGCC_G Cuts at: 1537 1812 2634 3118 3164 3406 1537 Size: 275 822 484 46 242 4090 Fragments arranged by size: 4090 822 484 275 242 46 EarI CTCTTCn'nnn_ Cuts at: 1353 2285 2804 3889 1353 Size: 932 519 1085 3423 Fragments arranged by size: 3423 1085 932 519 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4546 4920 5066 387 Size: 1064 138 2957 374 146 1280 Fragments arranged by size: 2957 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2278 2278 Size: 5959 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2217 4022 4619 5406 5674 1239 Size: 393 585 1805 597 787 268 1524 Fragments arranged by size: 1805 1524 787 597 585 393 268 EcoO109I rG'GnC_Cy Cuts at: 2983 3032 3033 2983 Size: 49 1 5909 Fragments arranged by size: 5909 49 1 EcoRI G'AATT_C Cuts at: 3268 3646 3268 Size: 378 5581 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2989 2989 Size: 5959 FspI TGC'GCA Cuts at: 3011 3245 4465 3011 Size: 234 1220 4505 Fragments arranged by size: 4505 1220 234 FspAI rTGC'GCAy Cuts at: 3245 3245 Size: 5959 HaeII r_GCGC'y Cuts at: 1678 2267 2719 2755 2798 2906 5106 1678 Size: 589 452 36 43 108 2200 2531 Fragments arranged by size: 2531 2200 589 452 108 43 36 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4600 4632 4674 4706 1373 Size: 32 135 32 3028 32 42 32 2626 Fragments arranged by size: 3028 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2842 3303 3549 669 Size: 988 281 904 461 246 3079 Fragments arranged by size: 3079 988 904 461 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5959 HpaI GTT'AAC Cuts at: 3549 3549 Size: 5959 KasI G'GCGC_C Cuts at: 2715 2715 Size: 5959 KpnI G_GTAC'C Cuts at: 3284 3284 Size: 5959 MfeI C'AATT_G Cuts at: 3558 3558 Size: 5959 MluI A'CGCG_T Cuts at: 5835 5835 Size: 5959 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5073 5257 1305 Size: 3768 184 2007 Fragments arranged by size: 3768 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3182 10 Size: 54 1425 343 1350 2787 Fragments arranged by size: 2787 1425 1350 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2204 2366 2417 2693 2734 2774 Size: 648 843 195 162 51 276 41 40 Cuts at: 2774 2780 3917 4276 4435 518 Size: 6 1137 359 159 2042 Fragments arranged by size: 2042 1137 843 648 359 276 195 162 159 51 41 40 6 MspA1I CmG'CkG Cuts at: 1606 2463 2562 2659 2711 2743 2922 3153 3375 Size: 857 99 97 52 32 179 231 222 Cuts at: 3375 4052 5200 5445 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 857 677 245 231 222 179 99 97 52 32 NaeI GCC'GGC Cuts at: 2598 2617 2598 Size: 19 5940 NarI GG'CG_CC Cuts at: 2716 2716 Size: 5959 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4468 Fragments arranged by size: 4468 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5959 NgoMIV G'CCGG_C Cuts at: 2596 2615 2596 Size: 19 5940 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5959 NotI GC'GGCC_GC Cuts at: 3164 3406 3164 Size: 242 5717 NspI r_CATG'y Cuts at: 1132 1229 2014 2584 3317 4862 1132 Size: 97 785 570 733 1545 2229 Fragments arranged by size: 2229 1545 785 733 570 97 PasI CC'CwG_GG Cuts at: 1348 2086 1348 Size: 738 5221 PciI A'CATG_T Cuts at: 4858 4858 Size: 5959 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5959 PmeI GTTT'AAAC Cuts at: 3263 3263 Size: 5959 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4028 4060 5541 5573 4028 Size: 32 1481 32 4414 Fragments arranged by size: 4414 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2983 2983 Size: 5959 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5959 PsiI TTA'TAA Cuts at: 3529 3529 Size: 5959 PspOMI G'GGCC_C Cuts at: 2104 3032 2104 Size: 928 5031 PstI C_TGCA'G Cuts at: 830 3311 830 Size: 2481 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5959 PvuII CAG'CTG Cuts at: 2711 2922 2711 Size: 211 5748 SacI G_AGCT'C Cuts at: 2280 2280 Size: 5959 SacII CC_GC'GG Cuts at: 2563 2563 Size: 5959 SalI G'TCGA_C Cuts at: 1936 3301 1936 Size: 1365 4594 SapI GCTCTTCn'nnn_ Cuts at: 2285 2804 2285 Size: 519 5440 SbfI CC_TGCA'GG Cuts at: 3311 3311 Size: 5959 ScaI AGT'ACT Cuts at: 4207 5919 4207 Size: 1712 4247 SexAI A'CCwGG_T Cuts at: 2157 3255 2157 Size: 1098 4861 SfcI C'TryA_G Cuts at: 826 1045 3307 4442 5123 5314 826 Size: 219 2262 1135 681 191 1471 Fragments arranged by size: 2262 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2717 2717 Size: 5959 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5959 SmaI CCC'GGG Cuts at: 1872 3286 1872 Size: 1414 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4063 4964 5226 820 Size: 197 926 2120 901 262 1553 Fragments arranged by size: 2120 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3750 493 Size: 3257 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5959 SphI G_CATG'C Cuts at: 1132 3317 1132 Size: 2185 3774 SspI AAT'ATT Cuts at: 5 52 3883 5 Size: 47 3831 2081 Fragments arranged by size: 3831 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5959 StyI C'CwwG_G Cuts at: 513 1065 2004 3398 513 Size: 552 939 1394 3074 Fragments arranged by size: 3074 1394 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2497 3986 4003 4156 4341 4786 325 Size: 1255 917 1489 17 153 185 445 1498 Fragments arranged by size: 1498 1489 1255 917 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4205 5917 96 Size: 274 80 33 51 1443 2228 1712 138 Fragments arranged by size: 2228 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5919 5951 5919 Size: 32 5927 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5959 XbaI T'CTAG_A Cuts at: 3295 3295 Size: 5959 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5959 XmaI C'CCGG_G Cuts at: 1870 3284 1870 Size: 1414 4545 XmnI GAAnn'nnTTC Cuts at: 4088 4088 Size: 5959 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3767 276 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SanDI SfiI SrfI SwaI XcmI