Restriction map for
pFN21AB8461
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# NO CUT ENZYMES ( 163 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanII BbeI BbrPI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BsaAI BsaBI BsaI
BsaWI BseAI BsgI BsiWI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrFI BsrGI BssSI
Bst1107I BstBI BstEII BstPI BstYI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DpnII DraI DraIII DrdI Eam1105I
EarI Eco47III Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HgaI HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeI MaeIII MamI MboI
MfeI MflI MluI MroI MseI
MspA1I MunI NaeI NarI NcoI
NdeI NdeII NgoMIV NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI Sau3AI
SbfI ScaI SexAI SfaNI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TfiI Tru9I Tsp45I Tsp509I
Tth111I Van91I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 226 GACGTC GACGT^C
AcyI 226 GACGTC G[GA]^CG[CT]C
AfaI 93 GTAC GT^AC
AlwNI 421 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 468 GTGCTC G[AT]GC[AT]^C
AvaII 351 GGACC G^G[AT]CC
BalI 434 TGGCCA TGG^CCA
BbsI 229 GTCTTC GAAGAC(2/6)
BbvCI 535 CCTCAGC CCTCAGC(-5/-2)
BlpI 470 GCTCAGC GC^T[ACGT]AGC
BpmI 463 CTGGAG CTGGAG(16/14)
Bpu1102I 470 GCTCAGC GC^T[ACGT]AGC
BpuAI 229 GTCTTC GAAGAC(2/6)
BsaHI 226 GACGTC G[GA]^CG[CT]C
BseRI 571 CTCCTC GAGGAG(10/8)
BsiHKAI 468 GTGCTC G[AT]GC[AT]^C
BsiYI 426 CCTGGCACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 426 CCTGGCACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 197 GTCTC GTCTC(1/5)
BsmBI 196 CGTCTC CGTCTC(1/5)
BsrBI 205 CCGCTC CCGCTC(-3/-3)
BssHII 558 GCGCGC G^CGCGC
BstAPI 44 GCAAGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 470 GCTCAGC GC^T[ACGT]AGC
DraII 422 AGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 38 CCGTGG C^C[GA][CT]GG
EagI 202 CGGCCG C^GGCCG
EclXI 202 CGGCCG C^GGCCG
Eco52I 202 CGGCCG C^GGCCG
EcoO109I 422 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 437 CCAAGG C^C[AT][AT]GG
Hin1I 226 GACGTC G[GA]^CG[CT]C
HinfI 448 GACTC G^A[ACGT]TC
MluNI 434 TGGCCA TGG^CCA
MscI 434 TGGCCA TGG^CCA
MslI 330 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PleI 448 GACTC GAGTC(4/5)
PshAI 222 GACGGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 564 CTGCAG CTGCA^G
RsaI 93 GTAC GT^AC
SfcI 564 CTGCAG C^T[GA][CT]AG
SfiI 513 GGCCATCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 489 AGGCCT AGG^CCT
StyI 437 CCAAGG C^C[AT][AT]GG
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# TWO CUT ENZYMES ( 18 enzymes) #
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BanI 305 GGCACC G^G[CT][GA]CC
340 GGCACC
BbvI 549 GCAGC GCAGC(8/12)
566 GCAGC
BcnI 85 CCCGG CC^[GC]GG
519 CCGGG
BglI 514 GCCATCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
540 GCCCACGCGGC
BsiEI 192 CGACCG CG[GA][CT]^CG
202 CGGCCG
BsmFI 167 GGGAC GGGAC(10/14)
359 GGGAC
BspMI 49 ACCTGC ACCTGC(4/8)
353 ACCTGC
BsrI 432 ACTGG ACTGG(1/-1)
148 CCAGT
BstXI 125 CCAATGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
326 CCATCATCCTGG
DdeI 471 CTCAG C^T[ACGT]AG
536 CTCAG
EaeI 202 CGGCCG [CT]^GGCC[GA]
434 TGGCCA
FauI 417 CCCGC CCCGC(4/6)
357 GCGGG
MaeII 227 ACGT A^CGT
275 ACGT
MboII 393 GAAGA GAAGA(8/7)
230 TCTTC
NciI 85 CCCGG CC^[GC]GG
519 CCGGG
TaqI 377 TCGA T^CGA
506 TCGA
TseI 549 GCAGC G^C[AT]GC
566 GCAGC
TthHB8I 377 TCGA T^CGA
506 TCGA
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# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AccII AciI AluI BmyI BsaJI
Bsp1286I BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MnlI MspI
MvaI MvnI MwoI NlaIV Sau96I
ScrFI TspRI XcmI