Restriction map for
pFN21AB8460
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BclI BfaI BfrI BglI
BlnI BsaBI BsaHI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Cfr10I ClaI CpoI DraI DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI Hin1I HincII
HindII HindIII HpaI HphI KasI
KpnI Ksp632I KspI MaeI MaeIII
MamI MfeI MluI MroI MseI
MslI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tru9I Tsp45I Tsp509I Tth111I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccII 106 CGCG CG^CG
AviII 695 TGCGCA TGC^GCA
BalI 117 TGGCCA TGG^CCA
BbsI 622 GTCTTC GAAGAC(2/6)
BciVI 182 GTATCC GTATCC(6/5)
BglII 138 AGATCT A^GATCT
BlpI 487 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 487 GCTGAGC GC^T[ACGT]AGC
BpuAI 622 GTCTTC GAAGAC(2/6)
BsaAI 179 TACGTA [CT]AC^GT[GA]
BsaI 253 GAGACC GGTCTC(1/5)
BsaWI 650 ACCGGA [AT]^CCGG[AT]
BseRI 250 GAGGAG GAGGAG(10/8)
BsgI 319 CTGCAC GTGCAG(16/14)
BsmFI 741 GGGAC GGGAC(10/14)
BspHI 731 TCATGA T^CATGA
BspMI 575 GCAGGT ACCTGC(4/8)
BstUI 106 CGCG CG^CG
BstYI 138 AGATCT [GA]^GATC[CT]
CelII 487 GCTGAGC GC^T[ACGT]AGC
DpnII 139 GATC ^GATC
DraIII 607 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
FspI 695 TGCGCA TGC^GCA
MaeII 180 ACGT A^CGT
MboI 139 GATC ^GATC
MboII 623 TCTTC GAAGA(8/7)
MflI 138 AGATCT [GA]^GATC[CT]
MluNI 117 TGGCCA TGG^CCA
MscI 117 TGGCCA TGG^CCA
MvnI 106 CGCG CG^CG
NdeII 139 GATC ^GATC
RcaI 731 TCATGA T^CATGA
Sau3AI 139 GATC ^GATC
SfaNI 547 GATGC GCATC(5/9)
SmaI 232 CCCGGG CCC^GGG
SnaBI 179 TACGTA TAC^GTA
StuI 189 AGGCCT AGG^CCT
XcmI 155 CCAGCAGACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 138 AGATCT [GA]^GATC[CT]
XmaCI 232 CCCGGG C^CCGGG
XmaI 232 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 7 GTAC GT^AC
102 GTAC
AlwNI 463 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
676 CAGGCCCTG
AocI 258 CCTGAGG CC^T[ACGT]AGG
681 CCTGAGG
AspHI 109 GTGCTC G[AT]GC[AT]^C
214 GAGCAC
AvaI 232 CCCGGG C^[CT]CG[GA]G
430 CTCGGG
BsiHKAI 109 GTGCTC G[AT]GC[AT]^C
214 GAGCAC
BsoBI 232 CCCGGG C^[CT]CG[GA]G
430 CTCGGG
Bsu36I 258 CCTGAGG CC^T[ACGT]AGG
681 CCTGAGG
Eco81I 258 CCTGAGG CC^T[ACGT]AGG
681 CCTGAGG
EcoNI 348 CCTCCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
681 CCTGAGGAAGG
FauI 276 GCGGG CCCGC(4/6)
618 GCGGG
HaeII 227 AGCGCC [GA]GCGC^[CT]
344 AGCGCC
HgaI 24 GACGC GACGC(5/10)
314 GCGTC
HinfI 161 GACTC G^A[ACGT]TC
728 GACTC
PleI 161 GACTC GAGTC(4/5)
728 GACTC
RsaI 7 GTAC GT^AC
102 GTAC
TaqI 94 TCGA T^CGA
389 TCGA
TthHB8I 94 TCGA T^CGA
389 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AluI AvaII BanII BbvI
BcnI BmyI BpmI BsaJI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DraII EaeI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII ItaI MnlI
MspA1I MspI MvaI MwoI NciI
NlaIII NlaIV PflMI PstI PvuII
Sau96I ScrFI SfcI StyI TseI
TspRI Van91I