Restriction map for
pFN21AB8460
(Linear) (Six-base) MAPSORT of: KIBB8460.seq Check: 7964 from: 1 to: 756
KIBB8460.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 609 756
Size: 609 147
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 598 756
Size: 598 158
AlwNI CAG_nnn'CTG
Cuts at: 0 468 681 756
Size: 468 213 75
Fragments arranged by size:
468 213 75
AvaI C'yCGr_G
Cuts at: 0 232 430 756
Size: 232 198 326
Fragments arranged by size:
326 232 198
BanII G_rGCy'C
Cuts at: 0 437 494 646 756
Size: 437 57 152 110
Fragments arranged by size:
437 152 110 57
BbsI GAAGACnn'nnnn_
Cuts at: 0 615 756
Size: 615 141
BciVI GTATCCnnnnn_n'
Cuts at: 0 193 756
Size: 193 563
BglII A'GATC_T
Cuts at: 0 138 756
Size: 138 618
BlpI GC'TnA_GC
Cuts at: 0 488 756
Size: 488 268
BmrI ACTGGGnnnn_n'
Cuts at: 0 239 316 756
Size: 239 77 440
Fragments arranged by size:
440 239 77
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 586 696 756
Size: 109 477 110 60
Fragments arranged by size:
477 110 109 60
Bpu10I CC'TnA_GC
Cuts at: 0 436 756
Size: 436 320
BsaI GGTCTCn'nnnn_
Cuts at: 0 247 756
Size: 247 509
BsaAI yAC'GTr
Cuts at: 0 181 756
Size: 181 575
BsaWI w'CCGG_w
Cuts at: 0 650 756
Size: 650 106
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 265 756
Size: 265 491
BseYI C'CCAG_C
Cuts at: 0 267 461 756
Size: 267 194 295
Fragments arranged by size:
295 267 194
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 756
Size: 304 452
BsiHKAI G_wGCw'C
Cuts at: 0 113 218 756
Size: 113 105 538
Fragments arranged by size:
538 113 105
Bsp1286I G_dGCh'C
Cuts at: 0 113 218 437 494 646 756
Size: 113 105 219 57 152 110
Fragments arranged by size:
219 152 113 110 105 57
BspHI T'CATG_A
Cuts at: 0 731 756
Size: 731 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 566 756
Size: 566 190
BstYI r'GATC_y
Cuts at: 0 138 756
Size: 138 618
Bsu36I CC'TnA_GG
Cuts at: 0 259 682 756
Size: 259 423 74
Fragments arranged by size:
423 259 74
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 580 756
Size: 580 176
BtsI GCAGTG_nn'
Cuts at: 0 216 756
Size: 216 540
DraIII CAC_nnn'GTG
Cuts at: 0 612 756
Size: 612 144
EaeI y'GGCC_r
Cuts at: 0 117 329 504 756
Size: 117 212 175 252
Fragments arranged by size:
252 212 175 117
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 586 609 696 756
Size: 109 477 23 87 60
Fragments arranged by size:
477 109 87 60 23
EcoNI CCTnn'n_nnAGG
Cuts at: 0 352 685 756
Size: 352 333 71
Fragments arranged by size:
352 333 71
EcoO109I rG'GnC_Cy
Cuts at: 0 236 512 678 756
Size: 236 276 166 78
Fragments arranged by size:
276 236 166 78
FspI TGC'GCA
Cuts at: 0 697 756
Size: 697 59
HaeII r_GCGC'y
Cuts at: 0 231 348 756
Size: 231 117 408
Fragments arranged by size:
408 231 117
MscI TGG'CCA
Cuts at: 0 119 756
Size: 119 637
MspA1I CmG'CkG
Cuts at: 0 89 465 747 756
Size: 89 376 282 9
Fragments arranged by size:
376 282 89 9
PflMI CCAn_nnn'nTGG
Cuts at: 0 192 366 468 756
Size: 192 174 102 288
Fragments arranged by size:
288 192 174 102
PfoI T'CCnGG_A
Cuts at: 0 164 712 756
Size: 164 548 44
Fragments arranged by size:
548 164 44
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 433 465 756
Size: 433 32 291
Fragments arranged by size:
433 291 32
PstI C_TGCA'G
Cuts at: 0 223 290 410 577 756
Size: 223 67 120 167 179
Fragments arranged by size:
223 179 167 120 67
PvuII CAG'CTG
Cuts at: 0 89 465 747 756
Size: 89 376 282 9
Fragments arranged by size:
376 282 89 9
SfcI C'TryA_G
Cuts at: 0 80 219 286 406 573 756
Size: 80 139 67 120 167 183
Fragments arranged by size:
183 167 139 120 80 67
SmaI CCC'GGG
Cuts at: 0 234 756
Size: 234 522
SnaBI TAC'GTA
Cuts at: 0 181 756
Size: 181 575
StuI AGG'CCT
Cuts at: 0 191 756
Size: 191 565
StyI C'CwwG_G
Cuts at: 0 114 186 353 756
Size: 114 72 167 403
Fragments arranged by size:
403 167 114 72
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 162 756
Size: 162 594
XmaI C'CCGG_G
Cuts at: 0 232 756
Size: 232 524
Enzymes that do cut:
AcuI AlfI AlwNI AvaI BanII BbsI BciVI BglII
BlpI BmrI BpmI Bpu10I BsaI BsaAI BsaWI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BstYI Bsu36I
BtgZI BtsI DraIII EaeI Eco57MI EcoNI EcoO109I FspI
HaeII MscI MspA1I PflMI PfoI PsrI PstI PvuII
SfcI SmaI SnaBI StuI StyI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI
BcgI BclI BfrBI BglI Bme1580I BmgBI BmtI BplI
BpuEI BsaBI BsaHI BsaXI BsiEI BsiWI BsmI BsmBI
BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I BtgI ClaI CspCI DraI
DrdI EagI EarI EciI EcoICRI EcoRI EcoRV FalI
FseI FspAI Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MslI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI
SpeI SphI SrfI SspI SwaI TaqII TatI TstI
Tth111I XbaI XhoI XmnI ZraI