(Circular) (Six-base) MAPSORT of: pFN21AB8459.seq Check: 2882 from: 1 to: 6109 pFN21AB8459 6109 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3919 278 Size: 53 83 186 936 2383 2468 Fragments arranged by size: 2468 2383 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3452 1937 Size: 1515 4594 Acc65I G'GTAC_C Cuts at: 2200 3430 2200 Size: 1230 4879 AclI AA'CG_TT Cuts at: 1563 2708 3225 3273 4236 4609 1563 Size: 1145 517 48 963 373 3063 Fragments arranged by size: 3063 1145 963 517 373 48 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2088 2754 4172 5556 5824 1632 Size: 456 666 1418 1384 268 1917 Fragments arranged by size: 1917 1418 1384 666 456 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5912 AflIII A'CryG_T Cuts at: 1203 5008 5985 1203 Size: 3805 977 1327 Fragments arranged by size: 3805 1327 977 AgeI A'CCGG_T Cuts at: 4940 4940 Size: 6109 AhdI GACnn_n'nnGTC Cuts at: 4838 4838 Size: 6109 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6109 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3233 1184 Size: 2049 4060 AlwNI CAG_nnn'CTG Cuts at: 3852 5424 3852 Size: 1572 4537 ApaLI G'TGCA_C Cuts at: 4166 5322 4166 Size: 1156 4953 ApoI r'AATT_y Cuts at: 1493 2623 3384 3418 3644 3796 5905 1493 Size: 1130 761 34 226 152 2109 1697 Fragments arranged by size: 2109 1697 1130 761 226 152 34 AseI AT'TA_AT Cuts at: 160 1032 4663 160 Size: 872 3631 1606 Fragments arranged by size: 3631 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6109 AvaI C'yCGr_G Cuts at: 1870 1943 3434 1870 Size: 73 1491 4545 Fragments arranged by size: 4545 1491 73 BamHI G'GATC_C Cuts at: 3439 3806 3439 Size: 367 5742 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2200 3430 4885 618 Size: 325 217 627 413 1230 1455 1842 Fragments arranged by size: 1842 1455 1230 627 413 325 217 BanII G_rGCy'C Cuts at: 1391 2855 3428 1391 Size: 1464 573 4072 Fragments arranged by size: 4072 1464 573 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3179 928 Size: 971 1280 3858 Fragments arranged by size: 3858 1280 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3299 3333 4300 4334 1903 Size: 34 1362 34 967 34 3678 Fragments arranged by size: 3678 1362 967 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4001 5211 4001 Size: 1210 4899 BclI T'GATC_A Cuts at: 1548 1548 Size: 6109 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4720 136 Size: 107 122 71 631 605 3048 1525 Fragments arranged by size: 3048 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6104 6104 Size: 6109 BlpI GC'TnA_GC Cuts at: 3526 3526 Size: 6109 Bme1580I G_kGCm'C Cuts at: 1790 2296 4170 5326 1790 Size: 506 1874 1156 2573 Fragments arranged by size: 2573 1874 1156 506 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6109 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3840 4798 442 Size: 416 53 83 399 24 2423 958 1753 Fragments arranged by size: 2423 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6109 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2187 2229 2265 2887 3413 4769 1239 Size: 948 42 36 622 526 1356 2579 Fragments arranged by size: 2579 1356 948 622 526 42 36 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4234 5099 5397 4234 Size: 865 298 4946 Fragments arranged by size: 4946 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4772 882 Size: 617 3273 2219 Fragments arranged by size: 3273 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2527 3900 493 Size: 711 1323 1373 2702 Fragments arranged by size: 2702 1373 1323 711 BsaBI GATnn'nnATC Cuts at: 2155 6103 2155 Size: 3948 2161 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3916 4298 4933 275 Size: 53 83 186 936 2383 382 635 1451 Fragments arranged by size: 2383 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4542 4940 5214 5361 5772 4542 Size: 398 274 147 411 4879 Fragments arranged by size: 4879 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2650 2680 1178 Size: 30 165 30 1247 30 4607 Fragments arranged by size: 4607 1247 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2184 2226 2416 2419 1186 Size: 998 42 190 3 4876 Fragments arranged by size: 4876 998 190 42 3 BseYI C'CCAG_C Cuts at: 5312 5808 5312 Size: 496 5613 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5536 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3559 4320 5348 1540 Size: 275 186 1558 761 1028 2301 Fragments arranged by size: 2301 1558 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4170 4255 5326 4170 Size: 85 1071 4953 Fragments arranged by size: 4953 1071 85 BsmI GAATG_Cn' Cuts at: 3005 3322 3620 3713 3005 Size: 317 298 93 5401 Fragments arranged by size: 5401 317 298 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2296 2855 3428 4170 4255 5326 1391 Size: 399 506 559 573 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 573 559 506 399 85 BspEI T'CCGG_A Cuts at: 5772 5772 Size: 6109 BspHI T'CATG_A Cuts at: 3998 5728 3998 Size: 1730 4379 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3464 844 Size: 2620 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3996 1394 Size: 429 2173 3507 Fragments arranged by size: 3507 2173 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4604 4778 65 Size: 1183 3356 174 1396 Fragments arranged by size: 3356 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4753 4940 1700 Size: 110 2943 187 2869 Fragments arranged by size: 2943 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6109 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6109 BssSI C'ACGA_G Cuts at: 4169 5181 4169 Size: 1012 5097 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6109 BstBI TT'CG_AA Cuts at: 4913 4913 Size: 6109 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6109 BstYI r'GATC_y Cuts at: 1972 2326 3439 3806 4191 4208 5660 5746 5758 Size: 354 1113 367 385 17 1452 86 12 Cuts at: 5758 6104 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1113 385 367 354 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2088 513 Size: 552 939 84 4534 Fragments arranged by size: 4534 939 552 84 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5370 Fragments arranged by size: 5370 683 56 BtsI GCAGTG_nn' Cuts at: 3625 4419 4439 3625 Size: 794 20 5295 Fragments arranged by size: 5295 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6109 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2359 2394 529 Size: 35 1795 35 4244 Fragments arranged by size: 4244 1795 35 35 DraI TTT'AAA Cuts at: 2179 2188 2566 3124 3153 3413 2179 Size: 9 378 558 29 260 4875 Fragments arranged by size: 4875 558 378 260 29 9 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5116 809 Size: 507 3800 1802 Fragments arranged by size: 3800 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2091 2484 Size: 54 1425 50 275 18 177 84 393 Cuts at: 2484 3556 4445 6074 8 Size: 1072 889 1629 43 Fragments arranged by size: 1629 1425 1072 889 393 275 177 84 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3556 1537 Size: 275 1744 4090 Fragments arranged by size: 4090 1744 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2064 2950 3015 4039 1353 Size: 711 886 65 1024 3423 Fragments arranged by size: 3423 1024 886 711 65 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4696 5070 5216 387 Size: 1064 138 3107 374 146 1280 Fragments arranged by size: 3107 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2088 2187 2229 2265 2754 2887 3413 Size: 393 456 99 42 36 489 133 526 Cuts at: 3413 4172 4769 5556 5824 1239 Size: 759 597 787 268 1524 Fragments arranged by size: 1524 787 759 597 526 489 456 393 268 133 99 42 36 EcoNI CCTnn'n_nnAGG Cuts at: 2208 2259 2208 Size: 51 6058 EcoRI G'AATT_C Cuts at: 2623 3418 3796 2623 Size: 795 378 4936 Fragments arranged by size: 4936 795 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2356 2356 Size: 6109 FspI TGC'GCA Cuts at: 4615 4615 Size: 6109 HaeII r_GCGC'y Cuts at: 1678 2244 5256 1678 Size: 566 3012 2531 Fragments arranged by size: 3012 2531 566 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2750 2774 2782 2806 4750 Size: 32 135 32 1178 24 8 24 1944 Cuts at: 4750 4782 4824 4856 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1944 1178 135 42 32 32 32 32 24 24 8 HincII GTy'rAC Cuts at: 669 1657 1938 2706 3453 3699 669 Size: 988 281 768 747 246 3079 Fragments arranged by size: 3079 988 768 747 281 246 HindIII A'AGCT_T Cuts at: 748 2451 748 Size: 1703 4406 HpaI GTT'AAC Cuts at: 3699 3699 Size: 6109 KpnI G_GTAC'C Cuts at: 2204 3434 2204 Size: 1230 4879 MfeI C'AATT_G Cuts at: 3303 3708 3303 Size: 405 5704 MluI A'CGCG_T Cuts at: 5985 5985 Size: 6109 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2128 2440 5223 5407 1305 Size: 823 312 2783 184 2007 Fragments arranged by size: 2783 2007 823 312 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2093 10 Size: 54 1425 343 177 84 4026 Fragments arranged by size: 4026 1425 343 177 84 54 MslI CAynn'nnrTG Cuts at: 518 1166 4067 4426 4585 518 Size: 648 2901 359 159 2042 Fragments arranged by size: 2901 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2321 2654 3525 4202 5350 5595 1606 Size: 715 333 871 677 1148 245 2120 Fragments arranged by size: 2120 1148 871 715 677 333 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2088 513 Size: 552 939 84 4534 Fragments arranged by size: 4534 939 552 84 NdeI CA'TA_TG Cuts at: 387 387 Size: 6109 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6109 NotI GC'GGCC_GC Cuts at: 3556 3556 Size: 6109 NspI r_CATG'y Cuts at: 1132 1229 3467 5012 1132 Size: 97 2238 1545 2229 Fragments arranged by size: 2238 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6109 PciI A'CATG_T Cuts at: 5008 5008 Size: 6109 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6109 PmeI GTTT'AAAC Cuts at: 3413 3413 Size: 6109 PmlI CAC'GTG Cuts at: 2527 2527 Size: 6109 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4178 4210 5691 5723 4178 Size: 32 1481 32 4564 Fragments arranged by size: 4564 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6109 PsiI TTA'TAA Cuts at: 3679 3679 Size: 6109 PstI C_TGCA'G Cuts at: 830 3461 830 Size: 2631 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6109 PvuII CAG'CTG Cuts at: 2654 2654 Size: 6109 SalI G'TCGA_C Cuts at: 1936 3451 1936 Size: 1515 4594 SbfI CC_TGCA'GG Cuts at: 3461 3461 Size: 6109 ScaI AGT'ACT Cuts at: 4357 6069 4357 Size: 1712 4397 SfcI C'TryA_G Cuts at: 826 1045 3159 3457 4592 5273 5464 826 Size: 219 2114 298 1135 681 191 1471 Fragments arranged by size: 2114 1471 1135 681 298 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6109 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6109 SmaI CCC'GGG Cuts at: 1872 3436 1872 Size: 1564 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4213 5114 5376 820 Size: 197 926 2270 901 262 1553 Fragments arranged by size: 2270 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3900 493 Size: 3407 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6109 SphI G_CATG'C Cuts at: 1132 3467 1132 Size: 2335 3774 SspI AAT'ATT Cuts at: 5 52 2582 4033 5 Size: 47 2530 1451 2081 Fragments arranged by size: 2530 2081 1451 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6109 StyI C'CwwG_G Cuts at: 513 1065 2004 2088 2331 2373 3548 513 Size: 552 939 84 243 42 1175 3074 Fragments arranged by size: 3074 1175 939 552 243 84 42 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3052 3253 4136 4153 4306 4491 4936 Size: 1255 1472 201 883 17 153 185 445 Cuts at: 4936 325 Size: 1498 Fragments arranged by size: 1498 1472 1255 883 445 201 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4355 6067 96 Size: 274 80 33 51 1443 2378 1712 138 Fragments arranged by size: 2378 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6069 6101 6069 Size: 32 6077 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6109 XbaI T'CTAG_A Cuts at: 3445 3445 Size: 6109 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2965 2965 Size: 6109 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6109 XmaI C'CCGG_G Cuts at: 1870 3434 1870 Size: 1564 4545 XmnI GAAnn'nnTTC Cuts at: 4238 4238 Size: 6109 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3917 276 Size: 53 83 186 936 2383 2468 Fragments arranged by size: 2468 2383 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI