(Circular) (Six-base) MAPSORT of: pFN21AB8453.seq Check: 6001 from: 1 to: 5668 pFN21AB8453 5668 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2499 3478 278 Size: 53 83 186 936 963 979 2468 Fragments arranged by size: 2468 979 963 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3011 1937 Size: 1074 4594 Acc65I G'GTAC_C Cuts at: 2079 2989 2079 Size: 910 4758 AclI AA'CG_TT Cuts at: 1563 3795 4168 1563 Size: 2232 373 3063 Fragments arranged by size: 3063 2232 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3731 5115 5383 1632 Size: 2099 1384 268 1917 Fragments arranged by size: 2099 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5471 AflIII A'CryG_T Cuts at: 1203 2232 4567 5544 1203 Size: 1029 2335 977 1327 Fragments arranged by size: 2335 1327 1029 977 AgeI A'CCGG_T Cuts at: 4499 4499 Size: 5668 AhdI GACnn_n'nnGTC Cuts at: 4397 4397 Size: 5668 AleI CACnn'nnGTG Cuts at: 1166 2944 1166 Size: 1778 3890 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5668 AlwNI CAG_nnn'CTG Cuts at: 2470 2791 3411 4983 2470 Size: 321 620 1572 3155 Fragments arranged by size: 3155 1572 620 321 ApaI G_GGCC'C Cuts at: 2148 2876 2148 Size: 728 4940 ApaLI G'TGCA_C Cuts at: 3725 4881 3725 Size: 1156 4512 ApoI r'AATT_y Cuts at: 1493 2977 3203 3355 5464 1493 Size: 1484 226 152 2109 1697 Fragments arranged by size: 2109 1697 1484 226 152 AseI AT'TA_AT Cuts at: 160 1032 4222 160 Size: 872 3190 1606 Fragments arranged by size: 3190 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5668 AvaI C'yCGr_G Cuts at: 1870 1943 2120 2140 2193 2964 2993 1870 Size: 73 177 20 53 771 29 4545 Fragments arranged by size: 4545 771 177 73 53 29 20 BamHI G'GATC_C Cuts at: 2998 3365 2998 Size: 367 5301 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2079 2960 2989 4444 618 Size: 325 217 627 292 881 29 1455 1842 Fragments arranged by size: 1842 1455 881 627 325 292 217 29 BanII G_rGCy'C Cuts at: 1391 2041 2067 2148 2876 2987 1391 Size: 650 26 81 728 111 4072 Fragments arranged by size: 4072 728 650 111 81 26 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2164 2192 2260 928 Size: 971 265 28 68 4336 Fragments arranged by size: 4336 971 265 68 28 BbvCI CC'TCA_GC Cuts at: 2792 2825 2792 Size: 33 5635 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3859 3893 1903 Size: 34 50 34 1838 34 3678 Fragments arranged by size: 3678 1838 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3560 4770 3560 Size: 1210 4458 BclI T'GATC_A Cuts at: 1548 1548 Size: 5668 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4279 136 Size: 107 122 71 631 605 2607 1525 Fragments arranged by size: 2607 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5663 5663 Size: 5668 BlpI GC'TnA_GC Cuts at: 2126 3085 2126 Size: 959 4709 Bme1580I G_kGCm'C Cuts at: 1790 2148 2876 2965 3729 4885 1790 Size: 358 728 89 764 1156 2573 Fragments arranged by size: 2573 1156 764 728 358 89 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5668 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2315 2601 3399 Size: 416 53 83 399 24 898 286 798 Cuts at: 3399 4357 442 Size: 958 1753 Fragments arranged by size: 1753 958 898 798 416 399 286 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5668 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2060 2914 2060 Size: 854 4814 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2055 2237 2279 2795 2884 4328 1239 Size: 816 182 42 516 89 1444 2579 Fragments arranged by size: 2579 1444 816 516 182 89 42 Bpu10I CC'TnA_GC Cuts at: 2151 2408 2792 2825 2151 Size: 257 384 33 4994 Fragments arranged by size: 4994 384 257 33 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3793 4658 4956 3793 Size: 865 298 4505 Fragments arranged by size: 4505 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2771 4331 882 Size: 617 1272 1560 2219 Fragments arranged by size: 2219 1560 1272 617 BsaAI yAC'GTr Cuts at: 493 1204 2235 3459 493 Size: 711 1031 1224 2702 Fragments arranged by size: 2702 1224 1031 711 BsaBI GATnn'nnATC Cuts at: 5662 5662 Size: 5668 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2496 3475 3857 4492 Size: 53 83 186 936 963 979 382 635 Cuts at: 4492 275 Size: 1451 Fragments arranged by size: 1451 979 963 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4101 4499 4773 4920 5331 4101 Size: 398 274 147 411 4438 Fragments arranged by size: 4438 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5443 Fragments arranged by size: 5443 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2033 2903 1186 Size: 847 870 3951 Fragments arranged by size: 3951 870 847 BseYI C'CCAG_C Cuts at: 2353 2513 2731 2750 2952 2956 4871 5367 2353 Size: 160 218 19 202 4 1915 496 2654 Fragments arranged by size: 2654 1915 496 218 202 160 19 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2686 2784 1163 Size: 573 950 98 4047 Fragments arranged by size: 4047 950 573 98 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2740 3118 3879 4907 1540 Size: 275 186 739 378 761 1028 2301 Fragments arranged by size: 2301 1028 761 739 378 275 186 BsiHKAI G_wGCw'C Cuts at: 3729 3814 4885 3729 Size: 85 1071 4512 Fragments arranged by size: 4512 1071 85 BsmI GAATG_Cn' Cuts at: 3179 3272 3179 Size: 93 5575 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2041 2067 2148 2876 2965 2987 3729 Size: 399 251 26 81 728 89 22 742 Cuts at: 3729 3814 4885 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 728 399 251 89 85 81 26 22 BspEI T'CCGG_A Cuts at: 5331 5331 Size: 5668 BspHI T'CATG_A Cuts at: 3557 5287 3557 Size: 1730 3938 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3023 844 Size: 2179 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3555 1394 Size: 429 1732 3507 Fragments arranged by size: 3507 1732 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2175 4163 4337 65 Size: 1183 927 1988 174 1396 Fragments arranged by size: 1988 1396 1183 927 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2013 2523 4312 4499 1700 Size: 110 203 510 1789 187 2869 Fragments arranged by size: 2869 1789 510 203 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5668 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5668 BssSI C'ACGA_G Cuts at: 2563 3728 4740 2563 Size: 1165 1012 3491 Fragments arranged by size: 3491 1165 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5668 BstBI TT'CG_AA Cuts at: 4472 4472 Size: 5668 BstEII G'GTnAC_C Cuts at: 1184 2323 2818 1184 Size: 1139 495 4034 Fragments arranged by size: 4034 1139 495 BstYI r'GATC_y Cuts at: 1972 2998 3365 3750 3767 5219 5305 5317 5663 Size: 1026 367 385 17 1452 86 12 346 Cuts at: 5663 1972 Size: 1977 Fragments arranged by size: 1977 1452 1026 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2021 2337 2642 2706 513 Size: 552 956 316 305 64 3475 Fragments arranged by size: 3475 956 552 316 305 64 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4929 Fragments arranged by size: 4929 683 56 BtsI GCAGTG_nn' Cuts at: 2015 2187 3184 3978 3998 2015 Size: 172 997 794 20 3685 Fragments arranged by size: 3685 997 794 172 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5668 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5633 DraI TTT'AAA Cuts at: 2972 2972 Size: 5668 DraIII CAC_nnn'GTG Cuts at: 2320 2320 Size: 5668 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4675 809 Size: 507 3359 1802 Fragments arranged by size: 3359 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2397 2737 3115 Size: 54 1425 50 275 18 567 340 378 Cuts at: 3115 4004 5633 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 567 378 340 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2737 3115 1537 Size: 275 925 378 4090 Fragments arranged by size: 4090 925 378 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3598 1353 Size: 2245 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4255 4629 4775 387 Size: 1064 138 2666 374 146 1280 Fragments arranged by size: 2666 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2055 2237 2279 2795 2884 3731 4328 Size: 393 423 182 42 516 89 847 597 Cuts at: 4328 5115 5383 1239 Size: 787 268 1524 Fragments arranged by size: 1524 847 787 597 516 423 393 268 182 89 42 EcoO109I rG'GnC_Cy Cuts at: 2026 2115 2144 2145 2310 2872 2873 2893 2026 Size: 89 29 1 165 562 1 20 4801 Fragments arranged by size: 4801 562 165 89 29 20 1 1 EcoRI G'AATT_C Cuts at: 2977 3355 2977 Size: 378 5290 FspI TGC'GCA Cuts at: 4174 4174 Size: 5668 HaeII r_GCGC'y Cuts at: 1678 2459 4815 1678 Size: 781 2356 2531 Fragments arranged by size: 2531 2356 781 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4309 4341 4383 4415 1373 Size: 32 135 32 2737 32 42 32 2626 Fragments arranged by size: 2737 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3012 3258 669 Size: 988 281 1074 246 3079 Fragments arranged by size: 3079 1074 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5668 HpaI GTT'AAC Cuts at: 3258 3258 Size: 5668 KpnI G_GTAC'C Cuts at: 2083 2993 2083 Size: 910 4758 MfeI C'AATT_G Cuts at: 3267 3267 Size: 5668 MluI A'CGCG_T Cuts at: 5544 5544 Size: 5668 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4782 4966 1305 Size: 3477 184 2007 Fragments arranged by size: 3477 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2399 10 Size: 54 1425 343 567 3279 Fragments arranged by size: 3279 1425 567 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2180 2944 3626 3985 4144 518 Size: 648 1014 764 682 359 159 2042 Fragments arranged by size: 2042 1014 764 682 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2023 2104 2339 2485 2788 2956 3084 3761 Size: 417 81 235 146 303 168 128 677 Cuts at: 3761 4909 5154 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 417 303 245 235 168 146 128 81 NaeI GCC'GGC Cuts at: 2015 2015 Size: 5668 NcoI C'CATG_G Cuts at: 513 1065 2706 513 Size: 552 1641 3475 Fragments arranged by size: 3475 1641 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5668 NgoMIV G'CCGG_C Cuts at: 2013 2013 Size: 5668 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5668 NotI GC'GGCC_GC Cuts at: 2737 3115 2737 Size: 378 5290 NspI r_CATG'y Cuts at: 1132 1229 3026 4571 1132 Size: 97 1797 1545 2229 Fragments arranged by size: 2229 1797 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5668 PciI A'CATG_T Cuts at: 4567 4567 Size: 5668 PflMI CCAn_nnn'nTGG Cuts at: 1108 2320 1108 Size: 1212 4456 PfoI T'CCnGG_A Cuts at: 2243 2295 2243 Size: 52 5616 PmeI GTTT'AAAC Cuts at: 2972 2972 Size: 5668 PmlI CAC'GTG Cuts at: 2235 2235 Size: 5668 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3737 3769 5250 5282 3737 Size: 32 1481 32 4123 Fragments arranged by size: 4123 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2115 2310 2893 2115 Size: 195 583 4890 Fragments arranged by size: 4890 583 195 PshAI GACnn'nnGTC Cuts at: 1613 2264 2308 1613 Size: 651 44 4973 Fragments arranged by size: 4973 651 44 PsiI TTA'TAA Cuts at: 3238 3238 Size: 5668 PspOMI G'GGCC_C Cuts at: 2144 2872 2144 Size: 728 4940 PstI C_TGCA'G Cuts at: 830 2466 2748 2787 3020 830 Size: 1636 282 39 233 3478 Fragments arranged by size: 3478 1636 282 233 39 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5668 PvuII CAG'CTG Cuts at: 2788 2956 2788 Size: 168 5500 SacII CC_GC'GG Cuts at: 2024 2340 2024 Size: 316 5352 SalI G'TCGA_C Cuts at: 1936 3010 1936 Size: 1074 4594 SanDI GG'GwC_CC Cuts at: 2115 2310 2115 Size: 195 5473 SbfI CC_TGCA'GG Cuts at: 3020 3020 Size: 5668 ScaI AGT'ACT Cuts at: 3916 5628 3916 Size: 1712 3956 SfcI C'TryA_G Cuts at: 826 1045 2156 2462 2744 2783 2867 3016 4151 Size: 219 1111 306 282 39 84 149 1135 Cuts at: 4151 4832 5023 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1111 681 306 282 219 191 149 84 39 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5668 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5668 SmaI CCC'GGG Cuts at: 1872 2142 2195 2995 1872 Size: 270 53 800 4545 Fragments arranged by size: 4545 800 270 53 SmlI C'TyrA_G Cuts at: 820 1017 1943 3772 4673 4935 820 Size: 197 926 1829 901 262 1553 Fragments arranged by size: 1829 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3459 493 Size: 2966 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5668 SphI G_CATG'C Cuts at: 1132 3026 1132 Size: 1894 3774 SspI AAT'ATT Cuts at: 5 52 3592 5 Size: 47 3540 2081 Fragments arranged by size: 3540 2081 47 StuI AGG'CCT Cuts at: 1517 2292 1517 Size: 775 4893 StyI C'CwwG_G Cuts at: 513 1065 2450 2706 3107 513 Size: 552 1385 256 401 3074 Fragments arranged by size: 3074 1385 552 401 256 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2367 2948 3695 3712 3865 4050 4495 Size: 1255 787 581 747 17 153 185 445 Cuts at: 4495 325 Size: 1498 Fragments arranged by size: 1498 1255 787 747 581 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3914 5626 96 Size: 274 80 33 51 1443 1937 1712 138 Fragments arranged by size: 1937 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5628 5660 5628 Size: 32 5636 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5668 XbaI T'CTAG_A Cuts at: 3004 3004 Size: 5668 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2940 2940 Size: 5668 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5668 XmaI C'CCGG_G Cuts at: 1870 2140 2193 2993 1870 Size: 270 53 800 4545 Fragments arranged by size: 4545 800 270 53 XmnI GAAnn'nnTTC Cuts at: 3797 3797 Size: 5668 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2497 3476 276 Size: 53 83 186 936 963 979 2468 Fragments arranged by size: 2468 979 963 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SacI SapI SexAI SfiI SfoI SrfI SwaI