Restriction map for
pFN21AB8444
(Circular) (Six-base) MAPSORT of: pFN21AB8444.seq Check: 4267 from: 1 to: 5614
pFN21AB8444 5614 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2141 2141
Size: 5614
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3424 278
Size: 53 83 186 936 1888 2468
Fragments arranged by size:
2468 1888 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2957 1937
Size: 1020 4594
Acc65I G'GTAC_C
Cuts at: 2935 2935
Size: 5614
AclI AA'CG_TT
Cuts at: 1563 3741 4114 1563
Size: 2178 373 3063
Fragments arranged by size:
3063 2178 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2493 3677 5061 5329 1632
Size: 861 1184 1384 268 1917
Fragments arranged by size:
1917 1384 1184 861 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5417
AflIII A'CryG_T
Cuts at: 1203 4513 5490 1203
Size: 3310 977 1327
Fragments arranged by size:
3310 1327 977
AgeI A'CCGG_T
Cuts at: 4445 4445
Size: 5614
AhdI GACnn_n'nnGTC
Cuts at: 4343 4343
Size: 5614
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5614
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5614
AlwNI CAG_nnn'CTG
Cuts at: 2612 3357 4929 2612
Size: 745 1572 3297
Fragments arranged by size:
3297 1572 745
ApaLI G'TGCA_C
Cuts at: 3671 4827 3671
Size: 1156 4458
ApoI r'AATT_y
Cuts at: 1493 2234 2584 2923 3149 3301 5410 1493
Size: 741 350 339 226 152 2109 1697
Fragments arranged by size:
2109 1697 741 350 339 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4168 160
Size: 872 3136 1606
Fragments arranged by size:
3136 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5614
AvaI C'yCGr_G
Cuts at: 1870 1943 2939 1870
Size: 73 996 4545
Fragments arranged by size:
4545 996 73
BamHI G'GATC_C
Cuts at: 2601 2944 3311 2601
Size: 343 367 4904
Fragments arranged by size:
4904 367 343
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2935 4390 618
Size: 325 217 627 1148 1455 1842
Fragments arranged by size:
1842 1455 1148 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2933 1391
Size: 1542 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2430 928
Size: 971 531 4112
Fragments arranged by size:
4112 971 531
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3805 3839 1903
Size: 34 1868 34 3678
Fragments arranged by size:
3678 1868 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2328 3506 4716 2328
Size: 1178 1210 3226
Fragments arranged by size:
3226 1210 1178
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5614
BfrBI ATG'CAT
Cuts at: 2621 2621
Size: 5614
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4225 136
Size: 107 122 71 631 605 2553 1525
Fragments arranged by size:
2553 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5609 5609
Size: 5614
BlpI GC'TnA_GC
Cuts at: 3031 3031
Size: 5614
Bme1580I G_kGCm'C
Cuts at: 1790 2494 3675 4831 1790
Size: 704 1181 1156 2573
Fragments arranged by size:
2573 1181 1156 704
BmgBI CAC'GTC
Cuts at: 1318 2875 1318
Size: 1557 4057
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3345 4303 442
Size: 416 53 83 399 24 1928 958 1753
Fragments arranged by size:
1928 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5614
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2072 2279 4274 1239
Size: 833 207 1995 2579
Fragments arranged by size:
2579 1995 833 207
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3739 4604 4902 3739
Size: 865 298 4451
Fragments arranged by size:
4451 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2182 4277 882
Size: 617 683 2095 2219
Fragments arranged by size:
2219 2095 683 617
BsaAI yAC'GTr
Cuts at: 493 1204 3405 493
Size: 711 2201 2702
Fragments arranged by size:
2702 2201 711
BsaBI GATnn'nnATC
Cuts at: 5608 5608
Size: 5614
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3421 3803 4438 275
Size: 53 83 186 936 1888 382 635 1451
Fragments arranged by size:
1888 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2663 4047 4445 4719 4866 5277 2663
Size: 1384 398 274 147 411 3000
Fragments arranged by size:
3000 1384 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2629 2659 1178
Size: 30 165 30 1226 30 4133
Fragments arranged by size:
4133 1226 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2017 2637 1186
Size: 831 620 4163
Fragments arranged by size:
4163 831 620
BseYI C'CCAG_C
Cuts at: 4817 5313 4817
Size: 496 5118
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5041
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3064 3825 4853 1540
Size: 275 186 1063 761 1028 2301
Fragments arranged by size:
2301 1063 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3675 3760 4831 3675
Size: 85 1071 4458
Fragments arranged by size:
4458 1071 85
BsmI GAATG_Cn'
Cuts at: 3125 3218 3125
Size: 93 5521
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2494 2933 3675 3760 4831 1391
Size: 399 704 439 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 704 439 399 85
BspEI T'CCGG_A
Cuts at: 5277 5277
Size: 5614
BspHI T'CATG_A
Cuts at: 3503 5233 3503
Size: 1730 3884
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2141 2364 2436 2620 2662 2969 844
Size: 1297 223 72 184 42 307 3489
Fragments arranged by size:
3489 1297 307 223 184 72 42
BsrBI CCG'CTC
Cuts at: 1394 1823 2037 3501 1394
Size: 429 214 1464 3507
Fragments arranged by size:
3507 1464 429 214
BsrDI GCAATG_nn'
Cuts at: 65 1248 4109 4283 65
Size: 1183 2861 174 1396
Fragments arranged by size:
2861 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2056 2248 4258 4445 1700
Size: 110 246 192 2010 187 2869
Fragments arranged by size:
2869 2010 246 192 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5614
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5614
BssSI C'ACGA_G
Cuts at: 3674 4686 3674
Size: 1012 4602
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5614
BstBI TT'CG_AA
Cuts at: 4418 4418
Size: 5614
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5614
BstYI r'GATC_y
Cuts at: 1972 2271 2336 2448 2552 2601 2944 3311 3696
Size: 299 65 112 104 49 343 367 385
Cuts at: 3696 3713 5165 5251 5263 5609 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 343 299 112
104 86 65 49 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2101 513
Size: 552 1036 4026
Fragments arranged by size:
4026 1036 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4875
Fragments arranged by size:
4875 683 56
BtsI GCAGTG_nn'
Cuts at: 3130 3924 3944 3130
Size: 794 20 4800
Fragments arranged by size:
4800 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5614
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5579
DraI TTT'AAA
Cuts at: 2437 2918 2437
Size: 481 5133
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4621 809
Size: 507 3305 1802
Fragments arranged by size:
3305 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2506 2821 3061
Size: 54 1425 50 275 18 676 315 240
Cuts at: 3061 3950 5579 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 676 315 275 240 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3061 1537
Size: 275 1249 4090
Fragments arranged by size:
4090 1249 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3544 1353
Size: 2191 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2002 2029 4201 4575 4721 387
Size: 1064 138 413 27 2172 374 146 1280
Fragments arranged by size:
2172 1280 1064 413 374 146 138 27
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2072 2279 2493 3677 4274 5061 5329
Size: 393 440 207 214 1184 597 787 268
Cuts at: 5329 1239
Size: 1524
Fragments arranged by size:
1524 1184 787 597 440 393 268 214
207
EcoNI CCTnn'n_nnAGG
Cuts at: 2014 2014
Size: 5614
EcoRI G'AATT_C
Cuts at: 2584 2923 3301 2584
Size: 339 378 4897
Fragments arranged by size:
4897 378 339
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2785 2785
Size: 5614
FspI TGC'GCA
Cuts at: 4120 4120
Size: 5614
HaeII r_GCGC'y
Cuts at: 1678 2101 4761 1678
Size: 423 2660 2531
Fragments arranged by size:
2660 2531 423
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2629 2661 4255 4287 4329
Size: 32 135 32 1057 32 1594 32 42
Cuts at: 4329 4361 1373
Size: 32 2626
Fragments arranged by size:
2626 1594 1057 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2958 3204 669
Size: 988 281 1020 246 3079
Fragments arranged by size:
3079 1020 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2543 748
Size: 1795 3819
HpaI GTT'AAC
Cuts at: 3204 3204
Size: 5614
KpnI G_GTAC'C
Cuts at: 2939 2939
Size: 5614
MfeI C'AATT_G
Cuts at: 3213 3213
Size: 5614
MluI A'CGCG_T
Cuts at: 5490 5490
Size: 5614
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2613 4728 4912 1305
Size: 1308 2115 184 2007
Fragments arranged by size:
2115 2007 1308 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2823 10
Size: 54 1425 343 991 2801
Fragments arranged by size:
2801 1425 991 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2807 3572 3931 4090 518
Size: 648 1641 765 359 159 2042
Fragments arranged by size:
2042 1641 765 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3030 3707 4855 5100 1606
Size: 1424 677 1148 245 2120
Fragments arranged by size:
2120 1424 1148 677 245
NaeI GCC'GGC
Cuts at: 2250 2250
Size: 5614
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5062
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5614
NgoMIV G'CCGG_C
Cuts at: 2248 2248
Size: 5614
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5614
NotI GC'GGCC_GC
Cuts at: 3061 3061
Size: 5614
NsiI A_TGCA'T
Cuts at: 2623 2623
Size: 5614
NspI r_CATG'y
Cuts at: 1132 1229 2972 4517 1132
Size: 97 1743 1545 2229
Fragments arranged by size:
2229 1743 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2138 1348
Size: 790 4824
PciI A'CATG_T
Cuts at: 4513 4513
Size: 5614
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2281 1108
Size: 1173 4441
PmeI GTTT'AAAC
Cuts at: 2918 2918
Size: 5614
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3683 3715 5196 5228 3683
Size: 32 1481 32 4069
Fragments arranged by size:
4069 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5614
PsiI TTA'TAA
Cuts at: 3184 3184
Size: 5614
PstI C_TGCA'G
Cuts at: 830 2659 2966 830
Size: 1829 307 3478
Fragments arranged by size:
3478 1829 307
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5614
RsrII CG'GwC_CG
Cuts at: 2032 2032
Size: 5614
SalI G'TCGA_C
Cuts at: 1936 2956 1936
Size: 1020 4594
SbfI CC_TGCA'GG
Cuts at: 2966 2966
Size: 5614
ScaI AGT'ACT
Cuts at: 3862 5574 3862
Size: 1712 3902
SfcI C'TryA_G
Cuts at: 826 1045 2361 2655 2962 4097 4778 4969 826
Size: 219 1316 294 307 1135 681 191 1471
Fragments arranged by size:
1471 1316 1135 681 307 294 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5614
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5614
SmaI CCC'GGG
Cuts at: 1872 2941 1872
Size: 1069 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3718 4619 4881 820
Size: 197 926 1775 901 262 1553
Fragments arranged by size:
1775 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3405 493
Size: 2912 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5614
SphI G_CATG'C
Cuts at: 1132 2972 1132
Size: 1840 3774
SspI AAT'ATT
Cuts at: 5 52 3538 5
Size: 47 3486 2081
Fragments arranged by size:
3486 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5614
StyI C'CwwG_G
Cuts at: 513 1065 2769 3053 513
Size: 552 1704 284 3074
Fragments arranged by size:
3074 1704 552 284
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2365 3641 3658 3811 3996 4441 325
Size: 1255 785 1276 17 153 185 445 1498
Fragments arranged by size:
1498 1276 1255 785 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3860 5572 96
Size: 274 80 33 51 1443 1883 1712 138
Fragments arranged by size:
1883 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5574 5606 5574
Size: 32 5582
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5614
XbaI T'CTAG_A
Cuts at: 2950 2950
Size: 5614
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5614
XmaI C'CCGG_G
Cuts at: 1870 2939 1870
Size: 1069 4545
XmnI GAAnn'nnTTC
Cuts at: 3743 3743
Size: 5614
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3422 276
Size: 53 83 186 936 1888 2468
Fragments arranged by size:
2468 1888 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI RsrII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoO109I EcoRV FseI FspAI KasI NarI NruI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI