Restriction map for
pFN21AB8436
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BalI BamHI BanI BbeI
BbsI BbvCI BcgI BfrI BlnI
BlpI Bpu1102I BpuAI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmAI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraII DraIII
EagI Eam1105I EarI EclXI Eco52I
Eco81I EcoO109I EcoO65I EcoRV EcoT22I
FseI FspI HindIII HpaI KasI
KpnI Ksp632I KspI MfeI MluI
MluNI MroI MscI MspA1I MunI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacII
SapI SbfI ScaI SexAI SfcI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 865 GTCGAC GT^[AC][GT]AC
AccII 496 CGCG CG^CG
AcsI 919 GAATTC [GA]^AATT[CT]
AcyI 103 GACGCC G[GA]^CG[CT]C
Aor51HI 690 AGCGCT AGC^GCT
ApoI 919 GAATTC [GA]^AATT[CT]
BanII 115 GAGCTC G[GA]GC[CT]^C
BbrPI 984 CACGTG CAC^GTG
BglI 256 GCCATGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 828 AGATCT A^GATCT
BsaAI 984 CACGTG [CT]AC^GT[GA]
BsaBI 174 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 103 GACGCC G[GA]^CG[CT]C
BsmI 427 GAATGC GAATGC(1/-1)
BspMI 515 ACCTGC ACCTGC(4/8)
BsrBI 76 GAGCGG CCGCTC(-3/-3)
BsrI 1047 CCAGT ACTGG(1/-1)
BssSI 715 CTCGTG CACGAG(-5/-1)
BstUI 496 CGCG CG^CG
DrdI 3 GACTCTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 254 CGGCCA [CT]^GGCC[GA]
Eco47III 690 AGCGCT AGC^GCT
EcoNI 30 CCTGAGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 919 GAATTC G^AATTC
HaeII 690 AGCGCT [GA]GCGC^[CT]
HgaI 103 GACGC GACGC(5/10)
Hin1I 103 GACGCC G[GA]^CG[CT]C
HincII 865 GTCGAC GT[CT]^[GA]AC
HindII 865 GTCGAC GT[CT]^[GA]AC
MamI 174 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 252 CACGGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 496 CGCG CG^CG
NdeI 380 CATATG CA^TATG
PmaCI 984 CACGTG CAC^GTG
PmlI 984 CACGTG CAC^GTG
SacI 115 GAGCTC GAGCT^C
SalI 865 GTCGAC G^TCGAC
SfiI 255 GGCCATGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
XbaI 834 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AfaI 158 GTAC GT^AC
1065 GTAC
AspHI 115 GAGCTC G[AT]GC[AT]^C
310 GAGCAC
AvaI 133 CTCGGG C^[CT]CG[GA]G
914 CCCGAG
BciVI 196 GGATAC GTATCC(6/5)
647 GGATAC
BclI 269 TGATCA T^GATCA
705 TGATCA
BcnI 53 CCCGG CC^[GC]GG
111 CCGGG
BmyI 115 GAGCTC G[AGT]GC[ACT]^C
310 GAGCAC
BpmI 1045 CTCCAG CTGGAG(16/14)
7 CTGGAG
BsiHKAI 115 GAGCTC G[AT]GC[AT]^C
310 GAGCAC
BsoBI 133 CTCGGG C^[CT]CG[GA]G
914 CCCGAG
Bsp1286I 115 GAGCTC G[AGT]GC[ACT]^C
310 GAGCAC
BstF5I 183 CATCC GGATG(2/0)
1041 CATCC
BstYI 293 AGATCC [GA]^GATC[CT]
828 AGATCT
CfoI 691 GCGC GCG^C
989 GCGC
DsaI 186 CCATGG C^C[GA][CT]GG
278 CCATGG
FauI 107 CCCGC CCCGC(4/6)
99 GCGGG
FbaI 269 TGATCA T^GATCA
705 TGATCA
FokI 183 CATCC GGATG(9/13)
1041 CATCC
HapII 54 CCGG C^CGG
111 CCGG
HhaI 691 GCGC GCG^C
989 GCGC
HinP1I 691 GCGC G^CGC
989 GCGC
HpaII 54 CCGG C^CGG
111 CCGG
HphI 224 TCACC GGTGA(8/7)
668 TCACC
MaeII 160 ACGT A^CGT
985 ACGT
MaeIII 667 GTCAC ^GT[ACGT]AC
959 GTGAC
MflI 293 AGATCC [GA]^GATC[CT]
828 AGATCT
MseI 482 TTAA T^TAA
817 TTAA
MspI 54 CCGG C^CGG
111 CCGG
NciI 53 CCCGG CC^[GC]GG
111 CCGGG
NcoI 186 CCATGG C^CATGG
278 CCATGG
RsaI 158 GTAC GT^AC
1065 GTAC
SfaNI 388 GATGC GCATC(5/9)
901 GATGC
SmlI 118 CTCAAG C^T[CT][GA]AG
289 CTCAAG
Tru9I 482 TTAA T^TAA
817 TTAA
Tsp45I 667 GTCAC ^GT[GC]AC
959 GTGAC
TspRI 652 CACTG CA[GC]TG[ACGT][ACGT]^
957 CAGTG
XhoII 293 AGATCC [GA]^GATC[CT]
828 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AluI AlwI Asp700I AvaII
BbvI BfaI BsaJI BseRI BsiYI
BslI BsmFI BssKI BstNI Cac8I
Cfr13I DdeI DpnII EcoRII EcoT14I
Fnu4HI HaeIII HinfI ItaI MaeI
MboI MboII MnlI MvaI MwoI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI StyI TaqI TfiI
TseI Tsp509I TthHB8I XmnI