(Circular) (Six-base) MAPSORT of: pFN21AB8436.seq Check: 4876 from: 1 to: 5782 pFN21AB8436 5782 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3592 278 Size: 53 83 186 936 2056 2468 Fragments arranged by size: 2468 2056 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2871 3125 1937 Size: 934 254 4594 Fragments arranged by size: 4594 934 254 Acc65I G'GTAC_C Cuts at: 3103 3103 Size: 5782 AclI AA'CG_TT Cuts at: 1563 3909 4282 1563 Size: 2346 373 3063 Fragments arranged by size: 3063 2346 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2954 3083 3845 5229 5497 1632 Size: 1322 129 762 1384 268 1917 Fragments arranged by size: 1917 1384 1322 762 268 129 AfeI AGC'GCT Cuts at: 2697 2697 Size: 5782 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5585 AflIII A'CryG_T Cuts at: 1203 4681 5658 1203 Size: 3478 977 1327 Fragments arranged by size: 3478 1327 977 AgeI A'CCGG_T Cuts at: 4613 4613 Size: 5782 AhdI GACnn_n'nnGTC Cuts at: 4511 4511 Size: 5782 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5782 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5782 AlwNI CAG_nnn'CTG Cuts at: 3525 5097 3525 Size: 1572 4210 ApaLI G'TGCA_C Cuts at: 3839 4995 3839 Size: 1156 4626 ApoI r'AATT_y Cuts at: 1493 2924 3091 3317 3469 5578 1493 Size: 1431 167 226 152 2109 1697 Fragments arranged by size: 2109 1697 1431 226 167 152 AseI AT'TA_AT Cuts at: 160 1032 4336 160 Size: 872 3304 1606 Fragments arranged by size: 3304 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5782 AvaI C'yCGr_G Cuts at: 1870 1943 2138 2919 3107 1870 Size: 73 195 781 188 4545 Fragments arranged by size: 4545 781 195 188 73 BamHI G'GATC_C Cuts at: 3112 3479 3112 Size: 367 5415 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3103 4558 618 Size: 325 217 627 1316 1455 1842 Fragments arranged by size: 1842 1455 1316 627 325 217 BanII G_rGCy'C Cuts at: 1391 2124 3101 1391 Size: 733 977 4072 Fragments arranged by size: 4072 977 733 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4811 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3973 4007 1903 Size: 34 2036 34 3678 Fragments arranged by size: 3678 2036 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2195 2646 3674 4884 2195 Size: 451 1028 1210 3093 Fragments arranged by size: 3093 1210 1028 451 BclI T'GATC_A Cuts at: 1548 2274 2710 1548 Size: 726 436 4620 Fragments arranged by size: 4620 726 436 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2267 4393 136 Size: 107 122 71 631 605 595 2126 1525 Fragments arranged by size: 2126 1525 631 605 595 122 107 71 BglII A'GATC_T Cuts at: 2833 5777 2833 Size: 2944 2838 BlpI GC'TnA_GC Cuts at: 3199 3199 Size: 5782 Bme1580I G_kGCm'C Cuts at: 1790 3843 4999 1790 Size: 2053 1156 2573 Fragments arranged by size: 2573 2053 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5782 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3513 4471 442 Size: 416 53 83 399 24 2096 958 1753 Fragments arranged by size: 2096 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5782 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2033 3035 4442 1239 Size: 794 1002 1407 2579 Fragments arranged by size: 2579 1407 1002 794 Bpu10I CC'TnA_GC Cuts at: 2036 2036 Size: 5782 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2108 2279 3907 4772 5070 2108 Size: 171 1628 865 298 2820 Fragments arranged by size: 2820 1628 865 298 171 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4445 882 Size: 617 2946 2219 Fragments arranged by size: 2946 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2991 3573 493 Size: 711 1787 582 2702 Fragments arranged by size: 2702 1787 711 582 BsaBI GATnn'nnATC Cuts at: 2183 5776 2183 Size: 3593 2189 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2109 3589 3971 4606 Size: 53 83 186 936 576 1480 382 635 Cuts at: 4606 275 Size: 1451 Fragments arranged by size: 1480 1451 936 635 576 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4215 4613 4887 5034 5445 4215 Size: 398 274 147 411 4552 Fragments arranged by size: 4552 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2351 2381 1178 Size: 30 165 30 948 30 4579 Fragments arranged by size: 4579 948 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2057 2794 2858 1186 Size: 871 737 64 4110 Fragments arranged by size: 4110 871 737 64 BseYI C'CCAG_C Cuts at: 4985 5481 4985 Size: 496 5286 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5209 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3232 3993 5021 1540 Size: 275 186 1231 761 1028 2301 Fragments arranged by size: 2301 1231 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2124 2319 3843 3928 4999 2124 Size: 195 1524 85 1071 2907 Fragments arranged by size: 2907 1524 1071 195 85 BsmI GAATG_Cn' Cuts at: 2438 3293 3386 2438 Size: 855 93 4834 Fragments arranged by size: 4834 855 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2124 2319 3101 3843 3928 4999 1391 Size: 399 334 195 782 742 85 1071 2174 Fragments arranged by size: 2174 1071 782 742 399 334 195 85 BspEI T'CCGG_A Cuts at: 5445 5445 Size: 5782 BspHI T'CATG_A Cuts at: 3671 5401 3671 Size: 1730 4052 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2529 3137 844 Size: 1685 608 3489 Fragments arranged by size: 3489 1685 608 BsrBI CCG'CTC Cuts at: 1394 1823 2083 3669 1394 Size: 429 260 1586 3507 Fragments arranged by size: 3507 1586 429 260 BsrDI GCAATG_nn' Cuts at: 65 1248 4277 4451 65 Size: 1183 3029 174 1396 Fragments arranged by size: 3029 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4426 4613 1700 Size: 110 2616 187 2869 Fragments arranged by size: 2869 2616 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5782 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5782 BssSI C'ACGA_G Cuts at: 2720 3842 4854 2720 Size: 1122 1012 3648 Fragments arranged by size: 3648 1122 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5782 BstBI TT'CG_AA Cuts at: 4586 4586 Size: 5782 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5782 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5782 BstYI r'GATC_y Cuts at: 1972 2298 2833 3112 3479 3864 3881 5333 5419 Size: 326 535 279 367 385 17 1452 86 Cuts at: 5419 5431 5777 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 535 385 367 346 326 279 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2191 2283 513 Size: 552 1126 92 4012 Fragments arranged by size: 4012 1126 552 92 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5043 Fragments arranged by size: 5043 683 56 BtsI GCAGTG_nn' Cuts at: 2656 3298 4092 4112 2656 Size: 642 794 20 4326 Fragments arranged by size: 4326 794 642 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5782 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5747 DraI TTT'AAA Cuts at: 3086 3086 Size: 5782 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2014 4789 809 Size: 507 698 2775 1802 Fragments arranged by size: 2775 1802 698 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2259 3229 4118 Size: 54 1425 50 275 18 429 970 889 Cuts at: 4118 5747 8 Size: 1629 43 Fragments arranged by size: 1629 1425 970 889 429 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3229 1537 Size: 275 1417 4090 Fragments arranged by size: 4090 1417 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3712 1353 Size: 2359 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2077 4369 4743 4889 387 Size: 1064 138 488 2292 374 146 1280 Fragments arranged by size: 2292 1280 1064 488 374 146 138 EcoICRI GAG'CTC Cuts at: 2122 2122 Size: 5782 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2033 2954 3035 3083 3845 4442 5229 Size: 393 401 921 81 48 762 597 787 Cuts at: 5229 5497 1239 Size: 268 1524 Fragments arranged by size: 1524 921 787 762 597 401 393 268 81 48 EcoNI CCTnn'n_nnAGG Cuts at: 2039 2039 Size: 5782 EcoRI G'AATT_C Cuts at: 2924 3091 3469 2924 Size: 167 378 5237 Fragments arranged by size: 5237 378 167 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2558 2782 2558 Size: 224 5558 FspI TGC'GCA Cuts at: 4288 4288 Size: 5782 HaeII r_GCGC'y Cuts at: 1678 2699 4929 1678 Size: 1021 2230 2531 Fragments arranged by size: 2531 2230 1021 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2351 2383 2538 2570 4423 Size: 32 135 32 779 32 155 32 1853 Cuts at: 4423 4455 4497 4529 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1853 779 155 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2872 3126 3372 669 Size: 988 281 934 254 246 3079 Fragments arranged by size: 3079 988 934 281 254 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5782 HpaI GTT'AAC Cuts at: 3372 3372 Size: 5782 KpnI G_GTAC'C Cuts at: 3107 3107 Size: 5782 MfeI C'AATT_G Cuts at: 3381 3381 Size: 5782 MluI A'CGCG_T Cuts at: 5658 5658 Size: 5782 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4896 5080 1305 Size: 3591 184 2007 Fragments arranged by size: 3591 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3960 Fragments arranged by size: 3960 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2261 3740 4099 4258 518 Size: 648 1095 1479 359 159 2042 Fragments arranged by size: 2042 1479 1095 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3198 3875 5023 5268 1606 Size: 1592 677 1148 245 2120 Fragments arranged by size: 2120 1592 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2191 2283 513 Size: 552 1126 92 4012 Fragments arranged by size: 4012 1126 552 92 NdeI CA'TA_TG Cuts at: 387 2386 387 Size: 1999 3783 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5782 NotI GC'GGCC_GC Cuts at: 3229 3229 Size: 5782 NspI r_CATG'y Cuts at: 1132 1229 3140 4685 1132 Size: 97 1911 1545 2229 Fragments arranged by size: 2229 1911 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5782 PciI A'CATG_T Cuts at: 4681 4681 Size: 5782 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5782 PfoI T'CCnGG_A Cuts at: 2419 2427 2419 Size: 8 5774 PmeI GTTT'AAAC Cuts at: 3086 3086 Size: 5782 PmlI CAC'GTG Cuts at: 2991 2991 Size: 5782 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3851 3883 5364 5396 3851 Size: 32 1481 32 4237 Fragments arranged by size: 4237 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5782 PsiI TTA'TAA Cuts at: 3352 3352 Size: 5782 PstI C_TGCA'G Cuts at: 830 3134 830 Size: 2304 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5782 SacI G_AGCT'C Cuts at: 2124 2124 Size: 5782 SalI G'TCGA_C Cuts at: 1936 2870 3124 1936 Size: 934 254 4594 Fragments arranged by size: 4594 934 254 SbfI CC_TGCA'GG Cuts at: 3134 3134 Size: 5782 ScaI AGT'ACT Cuts at: 4030 5742 4030 Size: 1712 4070 SfcI C'TryA_G Cuts at: 826 1045 3130 4265 4946 5137 826 Size: 219 2085 1135 681 191 1471 Fragments arranged by size: 2085 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2267 2267 Size: 5782 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5782 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5782 SmaI CCC'GGG Cuts at: 1872 3109 1872 Size: 1237 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2123 2294 3886 4787 5049 820 Size: 197 926 180 171 1592 901 262 1553 Fragments arranged by size: 1592 1553 926 901 262 197 180 171 SnaBI TAC'GTA Cuts at: 493 3573 493 Size: 3080 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5782 SphI G_CATG'C Cuts at: 1132 3140 1132 Size: 2008 3774 SspI AAT'ATT Cuts at: 5 52 3706 5 Size: 47 3654 2081 Fragments arranged by size: 3654 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5782 StyI C'CwwG_G Cuts at: 513 1065 2049 2191 2283 3221 513 Size: 552 984 142 92 938 3074 Fragments arranged by size: 3074 984 938 552 142 92 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3809 3826 3979 4164 4609 325 Size: 1255 2229 17 153 185 445 1498 Fragments arranged by size: 2229 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3069 4028 5740 Size: 274 80 33 51 1443 1092 959 1712 Cuts at: 5740 96 Size: 138 Fragments arranged by size: 1712 1443 1092 959 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2283 2315 5742 5774 2283 Size: 32 3427 32 2291 Fragments arranged by size: 3427 2291 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5782 XbaI T'CTAG_A Cuts at: 2839 3118 2839 Size: 279 5503 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5782 XmaI C'CCGG_G Cuts at: 1870 3107 1870 Size: 1237 4545 XmnI GAAnn'nnTTC Cuts at: 2252 2928 2939 3911 2252 Size: 676 11 972 4123 Fragments arranged by size: 4123 972 676 11 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3590 276 Size: 53 83 186 936 2056 2468 Fragments arranged by size: 2468 2056 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfoI SrfI SwaI XcmI