Restriction map for
pFN21AB8430
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# NO CUT ENZYMES ( 185 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiHKAI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstF5I BstPI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I Cfr13I ClaI CpoI DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FokI
FseI FspI HaeII HaeIII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeII MamI MfeI MluI MluNI
MroI MscI MunI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
Sau96I SbfI ScaI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccII 9 CGCG CG^CG
AcsI 297 GAATTT [GA]^AATT[CT]
AfaI 468 GTAC GT^AC
ApoI 297 GAATTT [GA]^AATT[CT]
BanII 421 GAGCCC G[GA]GC[CT]^C
BbsI 73 GAAGAC GAAGAC(2/6)
BmyI 421 GAGCCC G[AGT]GC[ACT]^C
BpuAI 73 GAAGAC GAAGAC(2/6)
BsiEI 14 CGACCG CG[GA][CT]^CG
BsiYI 425 CCCATGCACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 425 CCCATGCACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 370 GTCCC GGGAC(10/14)
Bsp1286I 421 GAGCCC G[AGT]GC[ACT]^C
BsrI 318 ACTGG ACTGG(1/-1)
BstUI 9 CGCG CG^CG
Cac8I 700 GCTGGC GC[ACGT]^[ACGT]GC
EarI 167 GAAGAG CTCTTC(1/4)
EcoT14I 147 CCTTGG C^C[AT][AT]GG
HapII 116 CCGG C^CGG
HpaII 116 CCGG C^CGG
HphI 379 GGTGA GGTGA(8/7)
Ksp632I 167 GAAGAG CTCTTC(1/4)
MaeIII 547 GTCAC ^GT[ACGT]AC
MslI 354 CATGGTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 698 CAGCTG C[AC]G^C[GT]G
MspI 116 CCGG C^CGG
MvnI 9 CGCG CG^CG
NlaIV 618 GGCTCC GG[ACGT]^[ACGT]CC
PleI 170 GAGTC GAGTC(4/5)
PvuII 698 CAGCTG CAG^CTG
RsaI 468 GTAC GT^AC
SfcI 492 CTACAG C^T[GA][CT]AG
SspI 282 AATATT AAT^ATT
StyI 147 CCTTGG C^C[AT][AT]GG
TfiI 96 GAATC G^A[AT]TC
Tsp45I 547 GTCAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AciI 17 CCGC CCGC(-3/-1)
10 GCGG
AluI 444 AGCT AG^CT
699 AGCT
Asp700I 152 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
625 GAAATAATTC
BfaI 58 CTAG C^TAG
481 CTAG
BglII 218 AGATCT A^GATCT
498 AGATCT
BssKI 373 CCTGG ^CC[ACGT]GG
543 CCAGG
BstNI 373 CCTGG CC^[AT]GG
543 CCAGG
BstYI 218 AGATCT [GA]^GATC[CT]
498 AGATCT
DpnII 219 GATC ^GATC
499 GATC
EcoRII 373 CCTGG ^CC[AT]GG
543 CCAGG
HinfI 96 GAATC G^A[ACGT]TC
170 GAGTC
MaeI 58 CTAG C^TAG
481 CTAG
MboI 219 GATC ^GATC
499 GATC
MflI 218 AGATCT [GA]^GATC[CT]
498 AGATCT
MvaI 373 CCTGG CC^[AT]GG
543 CCAGG
MwoI 10 GCGGCGACCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
252 GCAAATAGAGC
NdeII 219 GATC ^GATC
499 GATC
Sau3AI 219 GATC ^GATC
499 GATC
ScrFI 373 CCTGG CC^[ACGT]GG
543 CCAGG
TaqI 315 TCGA T^CGA
464 TCGA
TthHB8I 315 TCGA T^CGA
464 TCGA
XhoII 218 AGATCT [GA]^GATC[CT]
498 AGATCT
XmnI 152 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
625 GAAATAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
BbvI BpmI BsaJI DdeI Fnu4HI
ItaI MboII MnlI MseI NlaIII
Tru9I TseI Tsp509I TspRI