Restriction map for
pFN21AB8416
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# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspHI AvaI AviII AvrII BanII
BbeI BbrPI BbvCI BcgI BciVI
BglI BlnI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsoBI BspEI
BspLU11I BsrBI BsrFI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Cfr10I CpoI DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I FauI FseI FspI
HaeII KasI KpnI KspI MluI
MroI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NspI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacI SacII SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SpeI SphI Sse8387I
XbaI XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AcyI 42 GGCGTC G[GA]^CG[CT]C
AocI 955 CCTGAGG CC^T[ACGT]AGG
AseI 748 ATTAAT AT^TAAT
AsnI 748 ATTAAT AT^TAAT
AspI 1821 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 176 TGGCCA TGG^CCA
BanI 960 GGCACC G^G[CT][GA]CC
BbsI 2072 GAAGAC GAAGAC(2/6)
BclI 2559 TGATCA T^GATCA
BcnI 3115 CCGGG CC^[GC]GG
BglII 438 AGATCT A^GATCT
BlpI 1742 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 1742 GCTTAGC GC^T[ACGT]AGC
BpuAI 2072 GAAGAC GAAGAC(2/6)
BsaAI 1726 TACGTA [CT]AC^GT[GA]
BsaBI 2554 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 42 GGCGTC G[GA]^CG[CT]C
BseRI 1316 GAGGAG GAGGAG(10/8)
BsgI 554 GTGCAG GTGCAG(16/14)
BspMI 2673 ACCTGC ACCTGC(4/8)
BssSI 1313 CACGAG CACGAG(-5/-1)
BstXI 827 CCACAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 955 CCTGAGG CC^T[ACGT]AGG
CelII 1742 GCTTAGC GC^T[ACGT]AGC
CfoI 2652 GCGC GCG^C
DraII 3117 GGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 1356 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 955 CCTGAGG CC^T[ACGT]AGG
EcoO109I 3117 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 2380 GAATTC G^AATTC
EcoRV 795 GATATC GAT^ATC
FbaI 2559 TGATCA T^GATCA
HapII 3115 CCGG C^CGG
HgaI 43 GCGTC GACGC(5/10)
HhaI 2652 GCGC GCG^C
Hin1I 42 GGCGTC G[GA]^CG[CT]C
HinP1I 2652 GCGC G^CGC
HindIII 2756 AAGCTT A^AGCTT
HpaI 3018 GTTAAC GTT^AAC
HpaII 3115 CCGG C^CGG
MamI 2554 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2792 CAATTG C^AATTG
MluNI 176 TGGCCA TGG^CCA
MscI 176 TGGCCA TGG^CCA
MspI 3115 CCGG C^CGG
MunI 2792 CAATTG C^AATTG
NciI 3115 CCGGG CC^[GC]GG
PflFI 1821 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 3117 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 748 ATTAAT AT^TAAT
SalI 134 GTCGAC G^TCGAC
SapI 2143 GCTCTTC GCTCTTC(1/4)
SnaBI 1726 TACGTA TAC^GTA
StuI 1756 AGGCCT AGG^CCT
SwaI 2243 ATTTAAAT ATTT^AAAT
Tth111I 1821 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 134 GTCGAC GT^[AC][GT]AC
1729 GTAGAC
AciI 23 CCGC CCGC(-3/-1)
1813 CCGC
AflII 195 CTTAAG C^TTAAG
1335 CTTAAG
Asp700I 1223 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1300 GAAGAATTTC
BamHI 7 GGATCC G^GATCC
1845 GGATCC
BfrI 195 CTTAAG C^TTAAG
1335 CTTAAG
BmyI 531 GTGCCC G[AGT]GC[ACT]^C
1782 GGGCAC
BsaI 430 GAGACC GGTCTC(1/5)
1648 GAGACC
BsmI 2277 GAATGC GAATGC(1/-1)
2466 GAATGC
Bsp1286I 531 GTGCCC G[AGT]GC[ACT]^C
1782 GGGCAC
Bsp1407I 561 TGTACA T^GTACA
2615 TGTACA
BspDI 115 ATCGAT AT^CGAT
2867 ATCGAT
BspHI 270 TCATGA T^CATGA
3144 TCATGA
BsrDI 494 CATTGC GCAATG(2/0)
163 GCAATG
BsrGI 561 TGTACA T^GTACA
2615 TGTACA
ClaI 115 ATCGAT AT^CGAT
2867 ATCGAT
EaeI 20 TGGCCG [CT]^GGCC[GA]
176 TGGCCA
EcoT22I 122 ATGCAT ATGCA^T
3025 ATGCAT
MslI 159 CACAGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
978 CACAGCTATG
NsiI 122 ATGCAT ATGCA^T
3025 ATGCAT
PflMI 1027 CCAAATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1910 CCATACAGTGG
PstI 1172 CTGCAG CTGCA^G
1994 CTGCAG
RcaI 270 TCATGA T^CATGA
3144 TCATGA
SmlI 195 CTTAAG C^T[CT][GA]AG
1335 CTTAAG
SspBI 561 TGTACA T^GTACA
2615 TGTACA
SspI 2126 AATATT AAT^ATT
2353 AATATT
Van91I 1027 CCAAATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1910 CCATACAGTGG
XmnI 1223 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1300 GAAGAATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI AvaII BbvI BfaI BpmI
BsaJI BsiYI BslI BsmAI BsmFI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HincII HindII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII