(Circular) (Six-base) MAPSORT of: pFN21AB8416.seq Check: 8131 from: 1 to: 7861 pFN21AB8416 7861 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5671 278 Size: 53 83 186 936 4135 2468 Fragments arranged by size: 4135 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2140 3735 5204 1937 Size: 203 1595 1469 4594 Fragments arranged by size: 4594 1595 1469 203 Acc65I G'GTAC_C Cuts at: 5182 5182 Size: 7861 AclI AA'CG_TT Cuts at: 1563 5988 6361 1563 Size: 4425 373 3063 Fragments arranged by size: 4425 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2382 3825 4136 4262 4514 4842 5128 5924 Size: 750 1443 311 126 252 328 286 796 Cuts at: 5924 7308 7576 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1443 1384 796 750 328 311 286 268 252 126 AflII C'TTAA_G Cuts at: 820 1017 2200 3340 820 Size: 197 1183 1140 5341 Fragments arranged by size: 5341 1183 1140 197 AflIII A'CryG_T Cuts at: 1203 6760 7737 1203 Size: 5557 977 1327 Fragments arranged by size: 5557 1327 977 AgeI A'CCGG_T Cuts at: 6692 6692 Size: 7861 AhdI GACnn_n'nnGTC Cuts at: 6590 6590 Size: 7861 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7861 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7861 AlwNI CAG_nnn'CTG Cuts at: 2111 3376 3497 4709 5604 7176 2111 Size: 1265 121 1212 895 1572 2796 Fragments arranged by size: 2796 1572 1265 1212 895 121 ApaLI G'TGCA_C Cuts at: 5918 7074 5918 Size: 1156 6705 ApoI r'AATT_y Cuts at: 1493 2086 2574 2609 2649 3230 3308 3382 3436 Size: 593 488 35 40 581 78 74 54 Cuts at: 3436 3601 3977 4241 4385 5170 5396 5548 7657 Size: 165 376 264 144 785 226 152 2109 Cuts at: 7657 1493 Size: 1697 Fragments arranged by size: 2109 1697 785 593 581 488 376 264 226 165 152 144 78 74 54 40 35 AseI AT'TA_AT Cuts at: 160 1032 2754 6415 160 Size: 872 1722 3661 1606 Fragments arranged by size: 3661 1722 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7861 AvaI C'yCGr_G Cuts at: 1870 1943 5186 1870 Size: 73 3243 4545 Fragments arranged by size: 4545 3243 73 BamHI G'GATC_C Cuts at: 2012 3850 5191 5558 2012 Size: 1838 1341 367 4315 Fragments arranged by size: 4315 1838 1341 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2965 5182 6637 618 Size: 325 217 627 1178 2217 1455 1842 Fragments arranged by size: 2217 1842 1455 1178 627 325 217 BanII G_rGCy'C Cuts at: 1391 5180 1391 Size: 3789 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4084 928 Size: 971 2185 4705 Fragments arranged by size: 4705 2185 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6052 6086 1903 Size: 34 4115 34 3678 Fragments arranged by size: 4115 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5753 6963 5753 Size: 1210 6651 BclI T'GATC_A Cuts at: 1548 4564 1548 Size: 3016 4845 BfrBI ATG'CAT Cuts at: 2129 5032 2129 Size: 2903 4958 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6472 136 Size: 107 122 71 631 605 4800 1525 Fragments arranged by size: 4800 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2443 7856 2443 Size: 5413 2448 BlpI GC'TnA_GC Cuts at: 3748 5278 3748 Size: 1530 6331 Bme1580I G_kGCm'C Cuts at: 1790 2540 3791 5922 7078 1790 Size: 750 1251 2131 1156 2573 Fragments arranged by size: 2573 2131 1251 1156 750 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7861 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4090 5592 6550 Size: 416 53 83 399 24 2673 1502 958 Cuts at: 6550 442 Size: 1753 Fragments arranged by size: 2673 1753 1502 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7861 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2741 2930 5076 5112 6521 1239 Size: 1502 189 2146 36 1409 2579 Fragments arranged by size: 2579 2146 1502 1409 189 36 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5986 6851 7149 5986 Size: 865 298 6698 Fragments arranged by size: 6698 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2429 3647 6524 882 Size: 617 930 1218 2877 2219 Fragments arranged by size: 2877 2219 1218 930 617 BsaAI yAC'GTr Cuts at: 493 1204 3733 5652 493 Size: 711 2529 1919 2702 Fragments arranged by size: 2702 2529 1919 711 BsaBI GATnn'nnATC Cuts at: 4563 7855 4563 Size: 3292 4569 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2048 5668 6050 6685 Size: 53 83 186 936 515 3620 382 635 Cuts at: 6685 275 Size: 1451 Fragments arranged by size: 3620 1451 936 635 515 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6294 6692 6966 7113 7524 6294 Size: 398 274 147 411 6631 Fragments arranged by size: 6631 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2903 2933 1178 Size: 30 165 30 1500 30 6106 Fragments arranged by size: 6106 1500 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3336 1186 Size: 2150 5711 BseYI C'CCAG_C Cuts at: 7064 7560 7064 Size: 496 7365 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2580 1163 Size: 573 844 6444 Fragments arranged by size: 6444 844 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5311 6072 7100 1540 Size: 275 186 3310 761 1028 2301 Fragments arranged by size: 3310 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5922 6007 7078 5922 Size: 85 1071 6705 Fragments arranged by size: 6705 1071 85 BsmI GAATG_Cn' Cuts at: 4288 4477 5372 5465 4288 Size: 189 895 93 6684 Fragments arranged by size: 6684 895 189 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2540 3791 5180 5922 6007 7078 1391 Size: 399 750 1251 1389 742 85 1071 2174 Fragments arranged by size: 2174 1389 1251 1071 750 742 399 85 BspEI T'CCGG_A Cuts at: 7524 7524 Size: 7861 BspHI T'CATG_A Cuts at: 2275 5149 5750 7480 2275 Size: 2874 601 1730 2656 Fragments arranged by size: 2874 2656 1730 601 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4687 5216 844 Size: 3843 529 3489 Fragments arranged by size: 3843 3489 529 BsrBI CCG'CTC Cuts at: 1394 1823 5748 1394 Size: 429 3925 3507 Fragments arranged by size: 3925 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2175 2498 6356 6530 65 Size: 1183 927 323 3858 174 1396 Fragments arranged by size: 3858 1396 1183 927 323 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6505 6692 1700 Size: 110 4695 187 2869 Fragments arranged by size: 4695 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2566 4620 96 Size: 2470 2054 3337 Fragments arranged by size: 3337 2470 2054 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7861 BssSI C'ACGA_G Cuts at: 3318 5921 6933 3318 Size: 2603 1012 4246 Fragments arranged by size: 4246 2603 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7861 BstBI TT'CG_AA Cuts at: 6665 6665 Size: 7861 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7861 BstXI CCAn_nnnn'nTGG Cuts at: 2839 2839 Size: 7861 BstYI r'GATC_y Cuts at: 1972 2012 2302 2443 2777 3493 3850 5071 5191 Size: 40 290 141 334 716 357 1221 120 Cuts at: 5191 5558 5943 5960 7412 7498 7510 7856 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1221 716 385 367 357 346 334 290 141 120 86 40 17 12 Bsu36I CC'TnA_GG Cuts at: 2961 2961 Size: 7861 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 6370 Fragments arranged by size: 6370 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7122 Fragments arranged by size: 7122 683 56 BtsI GCAGTG_nn' Cuts at: 3573 4462 4545 4695 5377 6171 6191 3573 Size: 889 83 150 682 794 20 5243 Fragments arranged by size: 5243 889 794 682 150 83 20 ClaI AT'CG_AT Cuts at: 1554 2121 4873 1554 Size: 567 2752 4542 Fragments arranged by size: 4542 2752 567 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4941 4976 529 Size: 35 4377 35 3414 Fragments arranged by size: 4377 3414 35 35 DraI TTT'AAA Cuts at: 2772 3387 4251 5165 2772 Size: 615 864 914 5468 Fragments arranged by size: 5468 914 864 615 DraIII CAC_nnn'GTG Cuts at: 3366 3366 Size: 7861 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6868 809 Size: 507 5552 1802 Fragments arranged by size: 5552 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 2181 5308 Size: 54 1425 50 275 18 195 156 3127 Cuts at: 5308 6197 7826 8 Size: 889 1629 43 Fragments arranged by size: 3127 1629 1425 889 275 195 156 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5308 1537 Size: 275 3496 4090 Fragments arranged by size: 4090 3496 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2037 3096 3855 4155 4323 5791 1353 Size: 684 1059 759 300 168 1468 3423 Fragments arranged by size: 3423 1468 1059 759 684 300 168 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6448 6822 6968 387 Size: 1064 138 4859 374 146 1280 Fragments arranged by size: 4859 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2382 2741 2930 3825 4136 4262 4514 Size: 393 750 359 189 895 311 126 252 Cuts at: 4514 4842 5076 5112 5128 5924 6521 7308 7576 Size: 328 234 36 16 796 597 787 268 Cuts at: 7576 1239 Size: 1524 Fragments arranged by size: 1524 895 796 787 750 597 393 359 328 311 268 252 234 189 126 36 16 EcoO109I rG'GnC_Cy Cuts at: 5123 5123 Size: 7861 EcoRI G'AATT_C Cuts at: 4385 5170 5548 4385 Size: 785 378 6698 Fragments arranged by size: 6698 785 378 EcoRV GAT'ATC Cuts at: 2802 2802 Size: 7861 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2387 2387 Size: 7861 FspI TGC'GCA Cuts at: 6367 6367 Size: 7861 HaeII r_GCGC'y Cuts at: 1678 7008 1678 Size: 5330 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2200 2232 2428 2460 4322 Size: 32 135 32 628 32 196 32 1862 Cuts at: 4322 4354 6502 6534 6576 6608 1373 Size: 32 2148 32 42 32 2626 Fragments arranged by size: 2626 2148 1862 628 196 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2103 2141 5025 5205 5451 669 Size: 988 281 165 38 2884 180 246 3079 Fragments arranged by size: 3079 2884 988 281 246 180 165 38 HindIII A'AGCT_T Cuts at: 748 4761 748 Size: 4013 3848 HpaI GTT'AAC Cuts at: 5025 5451 5025 Size: 426 7435 KpnI G_GTAC'C Cuts at: 5186 5186 Size: 7861 MfeI C'AATT_G Cuts at: 4797 5460 4797 Size: 663 7198 MluI A'CGCG_T Cuts at: 7737 7737 Size: 7861 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3019 3540 3668 4507 6975 7159 1305 Size: 1714 521 128 839 2468 184 2007 Fragments arranged by size: 2468 2007 1714 839 521 184 128 MscI TGG'CCA Cuts at: 10 64 1489 1832 2183 10 Size: 54 1425 343 351 5688 Fragments arranged by size: 5688 1425 351 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2168 2987 5819 6178 6337 518 Size: 648 1002 819 2832 359 159 2042 Fragments arranged by size: 2832 2042 1002 819 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3899 4595 4637 5277 5954 7102 7347 1606 Size: 2293 696 42 640 677 1148 245 2120 Fragments arranged by size: 2293 2120 1148 696 677 640 245 42 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6370 Fragments arranged by size: 6370 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7861 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7861 NotI GC'GGCC_GC Cuts at: 5308 5308 Size: 7861 NsiI A_TGCA'T Cuts at: 2131 5034 2131 Size: 2903 4958 NspI r_CATG'y Cuts at: 1132 1229 5219 6764 1132 Size: 97 3990 1545 2229 Fragments arranged by size: 3990 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7861 PciI A'CATG_T Cuts at: 6760 6760 Size: 7861 PflMI CCAn_nnn'nTGG Cuts at: 1108 3038 3921 1108 Size: 1930 883 5048 Fragments arranged by size: 5048 1930 883 PmeI GTTT'AAAC Cuts at: 5165 5165 Size: 7861 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5930 5962 7443 7475 5930 Size: 32 1481 32 6316 Fragments arranged by size: 6316 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 5123 5123 Size: 7861 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7861 PsiI TTA'TAA Cuts at: 4061 5431 4061 Size: 1370 6491 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2835 2867 3900 3932 2835 Size: 32 1033 32 6764 Fragments arranged by size: 6764 1033 32 32 PstI C_TGCA'G Cuts at: 830 3181 4003 5213 830 Size: 2351 822 1210 3478 Fragments arranged by size: 3478 2351 1210 822 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7861 PvuII CAG'CTG Cuts at: 3899 4595 4637 3899 Size: 696 42 7123 Fragments arranged by size: 7123 696 42 SalI G'TCGA_C Cuts at: 1936 2139 5203 1936 Size: 203 3064 4594 Fragments arranged by size: 4594 3064 203 SapI GCTCTTCn'nnn_ Cuts at: 4155 4155 Size: 7861 SbfI CC_TGCA'GG Cuts at: 5213 5213 Size: 7861 ScaI AGT'ACT Cuts at: 6109 7821 6109 Size: 1712 6149 SfcI C'TryA_G Cuts at: 826 1045 3177 3999 4302 4683 4701 5209 6344 Size: 219 2132 822 303 381 18 508 1135 Cuts at: 6344 7025 7216 826 Size: 681 191 1471 Fragments arranged by size: 2132 1471 1135 822 681 508 381 303 219 191 18 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7861 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7861 SmaI CCC'GGG Cuts at: 1872 5188 1872 Size: 3316 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2200 3340 5965 6866 7128 820 Size: 197 926 257 1140 2625 901 262 1553 Fragments arranged by size: 2625 1553 1140 926 901 262 257 197 SnaBI TAC'GTA Cuts at: 493 3733 5652 493 Size: 3240 1919 2702 Fragments arranged by size: 3240 2702 1919 SpeI A'CTAG_T Cuts at: 152 152 Size: 7861 SphI G_CATG'C Cuts at: 1132 5219 1132 Size: 4087 3774 SspI AAT'ATT Cuts at: 5 52 4133 4360 5785 5 Size: 47 4081 227 1425 2081 Fragments arranged by size: 4081 2081 1425 227 47 StuI AGG'CCT Cuts at: 1517 3763 1517 Size: 2246 5615 StyI C'CwwG_G Cuts at: 513 1065 2004 2997 3096 3154 4722 5300 513 Size: 552 939 993 99 58 1568 578 3074 Fragments arranged by size: 3074 1568 993 939 578 552 99 58 SwaI ATTT'AAAT Cuts at: 4251 4251 Size: 7861 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2201 5888 5905 6058 6243 6688 325 Size: 1255 621 3687 17 153 185 445 1498 Fragments arranged by size: 3687 1498 1255 621 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2368 2566 3878 Size: 274 80 33 51 1443 391 198 1312 Cuts at: 3878 4620 6107 7819 96 Size: 742 1487 1712 138 Fragments arranged by size: 1712 1487 1443 1312 742 391 274 198 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7821 7853 7821 Size: 32 7829 Tth111I GACn'n_nGTC Cuts at: 1316 3829 1316 Size: 2513 5348 XbaI T'CTAG_A Cuts at: 5197 5197 Size: 7861 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7861 XmaI C'CCGG_G Cuts at: 1870 5186 1870 Size: 3316 4545 XmnI GAAnn'nnTTC Cuts at: 3232 3309 5990 3232 Size: 77 2681 5103 Fragments arranged by size: 5103 2681 77 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5669 276 Size: 53 83 186 936 4135 2468 Fragments arranged by size: 4135 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI XcmI