(Circular) (Six-base) MAPSORT of: pFN21AB8403.seq Check: 1511 from: 1 to: 6850 pFN21AB8403 6850 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2508 4660 278 Size: 53 83 186 936 972 2152 2468 Fragments arranged by size: 2468 2152 972 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2475 4193 1937 Size: 538 1718 4594 Fragments arranged by size: 4594 1718 538 Acc65I G'GTAC_C Cuts at: 4171 4171 Size: 6850 AclI AA'CG_TT Cuts at: 1563 4977 5350 1563 Size: 3414 373 3063 Fragments arranged by size: 3414 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2964 3522 4913 6297 6565 1632 Size: 1332 558 1391 1384 268 1917 Fragments arranged by size: 1917 1391 1384 1332 558 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6653 AflIII A'CryG_T Cuts at: 1203 2098 3103 3771 5749 6726 1203 Size: 895 1005 668 1978 977 1327 Fragments arranged by size: 1978 1327 1005 977 895 668 AgeI A'CCGG_T Cuts at: 5681 5681 Size: 6850 AhdI GACnn_n'nnGTC Cuts at: 5579 5579 Size: 6850 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6850 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6850 AlwNI CAG_nnn'CTG Cuts at: 2431 4019 4593 6165 2431 Size: 1588 574 1572 3116 Fragments arranged by size: 3116 1588 1572 574 ApaLI G'TGCA_C Cuts at: 4907 6063 4907 Size: 1156 5694 ApoI r'AATT_y Cuts at: 1493 2348 2386 2805 2826 3451 3479 3581 3871 Size: 855 38 419 21 625 28 102 290 Cuts at: 3871 4159 4385 4537 6646 1493 Size: 288 226 152 2109 1697 Fragments arranged by size: 2109 1697 855 625 419 290 288 226 152 102 38 28 21 AseI AT'TA_AT Cuts at: 160 1032 3035 3474 5404 160 Size: 872 2003 439 1930 1606 Fragments arranged by size: 2003 1930 1606 872 439 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6850 AvaI C'yCGr_G Cuts at: 1870 1943 4175 1870 Size: 73 2232 4545 Fragments arranged by size: 4545 2232 73 BamHI G'GATC_C Cuts at: 4180 4547 4180 Size: 367 6483 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4171 5626 618 Size: 325 217 627 2384 1455 1842 Fragments arranged by size: 2384 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2583 4169 1391 Size: 1192 1586 4072 Fragments arranged by size: 4072 1586 1192 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2301 928 Size: 971 402 5477 Fragments arranged by size: 5477 971 402 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2019 2053 3053 3087 5041 5075 1903 Size: 34 82 34 1000 34 1954 34 3678 Fragments arranged by size: 3678 1954 1000 82 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2855 3721 4742 5952 2855 Size: 866 1021 1210 3753 Fragments arranged by size: 3753 1210 1021 866 BclI T'GATC_A Cuts at: 1548 2053 1548 Size: 505 6345 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5461 136 Size: 107 122 71 631 605 3789 1525 Fragments arranged by size: 3789 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6845 6845 Size: 6850 BlpI GC'TnA_GC Cuts at: 2838 4267 2838 Size: 1429 5421 Bme1580I G_kGCm'C Cuts at: 1790 3092 4911 6067 1790 Size: 1302 1819 1156 2573 Fragments arranged by size: 2573 1819 1302 1156 BmgBI CAC'GTC Cuts at: 1318 2020 1318 Size: 702 6148 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2771 4581 5539 Size: 416 53 83 399 24 1354 1810 958 Cuts at: 5539 442 Size: 1753 Fragments arranged by size: 1810 1753 1354 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6850 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2890 2890 Size: 6850 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2616 3919 5510 1239 Size: 1377 1303 1591 2579 Fragments arranged by size: 2579 1591 1377 1303 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3837 4044 4975 5840 6138 3837 Size: 207 931 865 298 4549 Fragments arranged by size: 4549 931 865 298 207 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5513 882 Size: 617 4014 2219 Fragments arranged by size: 4014 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4641 493 Size: 711 3437 2702 Fragments arranged by size: 3437 2702 711 BsaBI GATnn'nnATC Cuts at: 6844 6844 Size: 6850 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2505 4657 5039 5674 Size: 53 83 186 936 972 2152 382 635 Cuts at: 5674 275 Size: 1451 Fragments arranged by size: 2152 1451 972 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5283 5681 5955 6102 6513 5283 Size: 398 274 147 411 5620 Fragments arranged by size: 5620 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6625 Fragments arranged by size: 6625 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3642 1186 Size: 2456 4394 BseYI C'CCAG_C Cuts at: 6053 6549 6053 Size: 496 6354 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6277 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4300 5061 6089 1540 Size: 275 186 2299 761 1028 2301 Fragments arranged by size: 2301 2299 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4911 4996 6067 4911 Size: 85 1071 5694 Fragments arranged by size: 5694 1071 85 BsmI GAATG_Cn' Cuts at: 4361 4454 4361 Size: 93 6757 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2583 3092 4169 4911 4996 6067 1391 Size: 399 793 509 1077 742 85 1071 2174 Fragments arranged by size: 2174 1077 1071 793 742 509 399 85 BspEI T'CCGG_A Cuts at: 6513 6513 Size: 6850 BspHI T'CATG_A Cuts at: 2050 3985 4739 6469 2050 Size: 1935 754 1730 2431 Fragments arranged by size: 2431 1935 1730 754 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2207 2505 4205 844 Size: 1363 298 1700 3489 Fragments arranged by size: 3489 1700 1363 298 BsrBI CCG'CTC Cuts at: 1394 1823 4737 1394 Size: 429 2914 3507 Fragments arranged by size: 3507 2914 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5345 5519 65 Size: 1183 4097 174 1396 Fragments arranged by size: 4097 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5494 5681 1700 Size: 110 3684 187 2869 Fragments arranged by size: 3684 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2936 96 Size: 2840 4010 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6850 BssSI C'ACGA_G Cuts at: 4910 5922 4910 Size: 1012 5838 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6850 BstBI TT'CG_AA Cuts at: 3178 5654 3178 Size: 2476 4374 BstEII G'GTnAC_C Cuts at: 1184 2194 1184 Size: 1010 5840 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 6850 BstYI r'GATC_y Cuts at: 1972 2104 2395 2531 4180 4547 4932 4949 6401 Size: 132 291 136 1649 367 385 17 1452 Cuts at: 6401 6487 6499 6845 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1649 1452 385 367 346 291 136 132 86 17 12 BstZ17I GTA'TAC Cuts at: 2476 2476 Size: 6850 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5359 Fragments arranged by size: 5359 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6111 Fragments arranged by size: 6111 683 56 BtsI GCAGTG_nn' Cuts at: 4366 5160 5180 4366 Size: 794 20 6036 Fragments arranged by size: 6036 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6850 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6815 DraI TTT'AAA Cuts at: 2644 2929 2991 3334 4154 2644 Size: 285 62 343 820 5340 Fragments arranged by size: 5340 820 343 285 62 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3518 5857 809 Size: 507 2202 2339 1802 Fragments arranged by size: 2339 2202 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4297 5186 6815 Size: 54 1425 50 275 18 2467 889 1629 Cuts at: 6815 8 Size: 43 Fragments arranged by size: 2467 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4297 1537 Size: 275 2485 4090 Fragments arranged by size: 4090 2485 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2411 4109 4780 1353 Size: 1058 1698 671 3423 Fragments arranged by size: 3423 1698 1058 671 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2030 5437 5811 5957 387 Size: 1064 138 441 3407 374 146 1280 Fragments arranged by size: 3407 1280 1064 441 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2616 2964 3522 3919 4913 5510 6297 Size: 393 984 348 558 397 994 597 787 Cuts at: 6297 6565 1239 Size: 268 1524 Fragments arranged by size: 1524 994 984 787 597 558 397 393 348 268 EcoO109I rG'GnC_Cy Cuts at: 3839 3839 Size: 6850 EcoRI G'AATT_C Cuts at: 3479 4159 4537 3479 Size: 680 378 5792 Fragments arranged by size: 5792 680 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2993 3575 2993 Size: 582 6268 FspI TGC'GCA Cuts at: 5356 5356 Size: 6850 HaeII r_GCGC'y Cuts at: 1678 5997 1678 Size: 4319 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2128 2160 3024 3056 3677 Size: 32 135 32 556 32 864 32 621 Cuts at: 3677 3709 5491 5523 5565 5597 1373 Size: 32 1782 32 42 32 2626 Fragments arranged by size: 2626 1782 864 621 556 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2684 3511 4194 4440 669 Size: 988 281 746 827 683 246 3079 Fragments arranged by size: 3079 988 827 746 683 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6850 HpaI GTT'AAC Cuts at: 4440 4440 Size: 6850 KpnI G_GTAC'C Cuts at: 4175 4175 Size: 6850 MfeI C'AATT_G Cuts at: 4449 4449 Size: 6850 MluI A'CGCG_T Cuts at: 6726 6726 Size: 6850 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2509 5964 6148 1305 Size: 1204 3455 184 2007 Fragments arranged by size: 3455 2007 1204 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5028 Fragments arranged by size: 5028 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3350 4808 5167 5326 518 Size: 648 2184 1458 359 159 2042 Fragments arranged by size: 2184 2042 1458 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2431 4266 4943 6091 6336 1606 Size: 825 1835 677 1148 245 2120 Fragments arranged by size: 2120 1835 1148 825 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5359 Fragments arranged by size: 5359 939 552 NdeI CA'TA_TG Cuts at: 387 3351 387 Size: 2964 3886 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6850 NotI GC'GGCC_GC Cuts at: 4297 4297 Size: 6850 NspI r_CATG'y Cuts at: 1132 1229 2102 3107 3620 3775 4208 5753 1132 Size: 97 873 1005 513 155 433 1545 2229 Fragments arranged by size: 2229 1545 1005 873 513 433 155 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6850 PciI A'CATG_T Cuts at: 2098 3103 3771 5749 2098 Size: 1005 668 1978 3199 Fragments arranged by size: 3199 1978 1005 668 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6850 PmeI GTTT'AAAC Cuts at: 4154 4154 Size: 6850 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4919 4951 6432 6464 4919 Size: 32 1481 32 5305 Fragments arranged by size: 5305 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3839 3839 Size: 6850 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6850 PsiI TTA'TAA Cuts at: 2325 4420 2325 Size: 2095 4755 PstI C_TGCA'G Cuts at: 830 4202 830 Size: 3372 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6850 PvuII CAG'CTG Cuts at: 2431 2431 Size: 6850 SalI G'TCGA_C Cuts at: 1936 4192 1936 Size: 2256 4594 SbfI CC_TGCA'GG Cuts at: 4202 4202 Size: 6850 ScaI AGT'ACT Cuts at: 5098 6810 5098 Size: 1712 5138 SexAI A'CCwGG_T Cuts at: 2722 2722 Size: 6850 SfcI C'TryA_G Cuts at: 826 1045 2308 2423 3948 4198 5333 6014 6205 Size: 219 1263 115 1525 250 1135 681 191 Cuts at: 6205 826 Size: 1471 Fragments arranged by size: 1525 1471 1263 1135 681 250 219 191 115 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6850 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6850 SmaI CCC'GGG Cuts at: 1872 4177 1872 Size: 2305 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3852 4059 4954 5855 6117 820 Size: 197 926 1909 207 895 901 262 1553 Fragments arranged by size: 1909 1553 926 901 895 262 207 197 SnaBI TAC'GTA Cuts at: 493 4641 493 Size: 4148 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6850 SphI G_CATG'C Cuts at: 1132 4208 1132 Size: 3076 3774 SspI AAT'ATT Cuts at: 5 52 2186 4774 5 Size: 47 2134 2588 2081 Fragments arranged by size: 2588 2134 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6850 StyI C'CwwG_G Cuts at: 513 1065 2004 4289 513 Size: 552 939 2285 3074 Fragments arranged by size: 3074 2285 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2815 4877 4894 5047 5232 5677 325 Size: 1255 1235 2062 17 153 185 445 1498 Fragments arranged by size: 2062 1498 1255 1235 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2095 2936 3416 Size: 274 80 33 51 1443 118 841 480 Cuts at: 3416 4087 5096 6808 96 Size: 671 1009 1712 138 Fragments arranged by size: 1712 1443 1009 841 671 480 274 138 118 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3583 3615 6810 6842 3583 Size: 32 3195 32 3591 Fragments arranged by size: 3591 3195 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2461 1316 Size: 1145 5705 XbaI T'CTAG_A Cuts at: 3623 4186 3623 Size: 563 6287 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6850 XmaI C'CCGG_G Cuts at: 1870 4175 1870 Size: 2305 4545 XmnI GAAnn'nnTTC Cuts at: 3360 3582 4979 3360 Size: 222 1397 5231 Fragments arranged by size: 5231 1397 222 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2506 4658 276 Size: 53 83 186 936 972 2152 2468 Fragments arranged by size: 2468 2152 972 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI