Restriction map for
pFN21AB8403
(Linear) (Six-base) MAPSORT of: KIBB8403.seq Check: 401 from: 1 to: 2145
KIBB8403.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
AatII G_ACGT'C
Cuts at: 0 503 2145
Size: 503 1642
AccI GT'mk_AC
Cuts at: 0 470 2145
Size: 470 1675
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 959 1517 2145
Size: 959 558 628
Fragments arranged by size:
959 628 558
AflIII A'CryG_T
Cuts at: 0 93 1098 1766 2145
Size: 93 1005 668 379
Fragments arranged by size:
1005 668 379 93
AlwNI CAG_nnn'CTG
Cuts at: 0 426 2014 2145
Size: 426 1588 131
Fragments arranged by size:
1588 426 131
ApoI r'AATT_y
Cuts at: 0 343 381 800 821 1446 1474 1576 1866
Size: 343 38 419 21 625 28 102 290
Cuts at: 1866 2145
Size: 279
Fragments arranged by size:
625 419 343 290 279 102 38 28
21
AseI AT'TA_AT
Cuts at: 0 1030 1469 2145
Size: 1030 439 676
Fragments arranged by size:
1030 676 439
BanII G_rGCy'C
Cuts at: 0 578 2145
Size: 578 1567
BbsI GAAGACnn'nnnn_
Cuts at: 0 296 2145
Size: 296 1849
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 14 48 1048 1082 2145
Size: 14 34 1000 34 1063
Fragments arranged by size:
1063 1000 34 34 14
BciVI GTATCCnnnnn_n'
Cuts at: 0 850 1716 2145
Size: 850 866 429
Fragments arranged by size:
866 850 429
BclI T'GATC_A
Cuts at: 0 48 2145
Size: 48 2097
BlpI GC'TnA_GC
Cuts at: 0 833 2145
Size: 833 1312
Bme1580I G_kGCm'C
Cuts at: 0 1087 2145
Size: 1087 1058
BmgBI CAC'GTC
Cuts at: 0 15 2145
Size: 15 2130
BmrI ACTGGGnnnn_n'
Cuts at: 0 766 2145
Size: 766 1379
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 885 2145
Size: 885 1260
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 611 1914 2145
Size: 611 1303 231
Fragments arranged by size:
1303 611 231
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1832 2039 2145
Size: 1832 207 106
Fragments arranged by size:
1832 207 106
BsaHI Gr'CG_yC
Cuts at: 0 500 2145
Size: 500 1645
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1637 2145
Size: 1637 508
Bsp1286I G_dGCh'C
Cuts at: 0 578 1087 2145
Size: 578 509 1058
Fragments arranged by size:
1058 578 509
BspHI T'CATG_A
Cuts at: 0 45 1980 2145
Size: 45 1935 165
Fragments arranged by size:
1935 165 45
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 202 500 2145
Size: 202 298 1645
Fragments arranged by size:
1645 298 202
BsrGI T'GTAC_A
Cuts at: 0 931 2145
Size: 931 1214
BstBI TT'CG_AA
Cuts at: 0 1173 2145
Size: 1173 972
BstEII G'GTnAC_C
Cuts at: 0 189 2145
Size: 189 1956
BstYI r'GATC_y
Cuts at: 0 99 390 526 2145
Size: 99 291 136 1619
Fragments arranged by size:
1619 291 136 99
BstZ17I GTA'TAC
Cuts at: 0 471 2145
Size: 471 1674
DraI TTT'AAA
Cuts at: 0 639 924 986 1329 2145
Size: 639 285 62 343 816
Fragments arranged by size:
816 639 343 285 62
DrdI GACnn_nn'nnGTC
Cuts at: 0 1513 2145
Size: 1513 632
EarI CTCTTCn'nnn_
Cuts at: 0 406 2104 2145
Size: 406 1698 41
Fragments arranged by size:
1698 406 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 25 2145
Size: 25 2120
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 611 959 1517 1914 2145
Size: 611 348 558 397 231
Fragments arranged by size:
611 558 397 348 231
EcoO109I rG'GnC_Cy
Cuts at: 0 1834 2145
Size: 1834 311
EcoRI G'AATT_C
Cuts at: 0 1474 2145
Size: 1474 671
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 988 1570 2145
Size: 988 582 575
Fragments arranged by size:
988 582 575
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 123 155 1019 1051 1672 1704 2145
Size: 123 32 864 32 621 32 441
Fragments arranged by size:
864 621 441 123 32 32 32
HincII GTy'rAC
Cuts at: 0 679 1506 2145
Size: 679 827 639
Fragments arranged by size:
827 679 639
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 504 2145
Size: 504 1641
MslI CAynn'nnrTG
Cuts at: 0 1345 2145
Size: 1345 800
MspA1I CmG'CkG
Cuts at: 0 426 2145
Size: 426 1719
NdeI CA'TA_TG
Cuts at: 0 1346 2145
Size: 1346 799
NspI r_CATG'y
Cuts at: 0 97 1102 1615 1770 2145
Size: 97 1005 513 155 375
Fragments arranged by size:
1005 513 375 155 97
PciI A'CATG_T
Cuts at: 0 93 1098 1766 2145
Size: 93 1005 668 379
Fragments arranged by size:
1005 668 379 93
PpuMI rG'GwC_Cy
Cuts at: 0 1834 2145
Size: 1834 311
PsiI TTA'TAA
Cuts at: 0 320 2145
Size: 320 1825
PvuII CAG'CTG
Cuts at: 0 426 2145
Size: 426 1719
SexAI A'CCwGG_T
Cuts at: 0 717 2145
Size: 717 1428
SfcI C'TryA_G
Cuts at: 0 303 418 1943 2145
Size: 303 115 1525 202
Fragments arranged by size:
1525 303 202 115
SmlI C'TyrA_G
Cuts at: 0 1847 2054 2145
Size: 1847 207 91
Fragments arranged by size:
1847 207 91
SspI AAT'ATT
Cuts at: 0 181 2145
Size: 181 1964
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 810 2145
Size: 810 1335
TatI w'GTAC_w
Cuts at: 0 90 931 1411 2082 2145
Size: 90 841 480 671 63
Fragments arranged by size:
841 671 480 90 63
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1578 1610 2145
Size: 1578 32 535
Fragments arranged by size:
1578 535 32
Tth111I GACn'n_nGTC
Cuts at: 0 456 2145
Size: 456 1689
XbaI T'CTAG_A
Cuts at: 0 1618 2145
Size: 1618 527
XmnI GAAnn'nnTTC
Cuts at: 0 1355 1577 2145
Size: 1355 222 568
Fragments arranged by size:
1355 568 222
ZraI GAC'GTC
Cuts at: 0 501 2145
Size: 501 1644
Enzymes that do cut:
AatII AccI AcuI AflIII AlwNI ApoI AseI BanII
BbsI BcgI BciVI BclI BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaHI BseRI Bsp1286I BspHI BspMI
BsrGI BstBI BstEII BstYI BstZ17I DraI DrdI EarI
EciI Eco57MI EcoO109I EcoRI FalI Hin4I HincII MmeI
MslI MspA1I NdeI NspI PciI PpuMI PsiI PvuII
SexAI SfcI SmlI SspI TaqII TatI TstI Tth111I
XbaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BbvCI BfrBI BglI BglII
BmtI Bpu10I BsaI BsaAI BsaBI BsaWI BsaXI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstXI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraIII EaeI EagI EcoICRI
EcoNI EcoRV FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MfeI MluI MscI NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI StuI StyI SwaI XcmI XhoI
XmaI